FastQCFastQC Report
Sat 31 Dec 2022
SRR5849793.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849793.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68195
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT7221.0587286457951464RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC5220.7654520126108952No Hit
TGCTAGGGGAAGATGGAGAAATTTAGCAGAGAACTAGAAGCTAAAAAAAGAA1280.18769704523792066No Hit
TGCAACTGGGCTGTAAACTGGAGTTAGCTCTAAATGCTTGATTATTTGGGTC1250.18329789574015692No Hit
GCTCGAGGGATCTGCTCCTGCGTTTCACGCATCTGCATACCTTTGGTTACTC1230.1803651294083144No Hit
GCCTCTGGGAGATGGTGGTAATTTTTATTCCCTTCTCTGCAATTTCAGCATA1220.17889874624239313No Hit
GCAATCGGGAGAATGGGAGGTTAGAGAAAGATTAATGCGACGGACTTAAAAT1030.15103746608988927No Hit
TCTCGTGGGAGAGACATAATTATCAGAATTTAAAAATCTGAGTAACCAGATT1020.14957108292396804No Hit
GGTAAAGGGAAGGTAGATTGTTTGAAGCAGAAGTCAGTTCTCCTGGAGCTCA1010.1481046997580468No Hit
ATAGTGGGGATGCAGAAAAAATTGAATCTTTATTGCTGATGGGAATATAAAA1010.1481046997580468No Hit
AACGAAGGGGGCCTGCGTAGCCGGCGGGCTGGCAGTGAGACTGACTGCGTCG1000.1466383165921255No Hit
GAGATAGGGAACCTTTTACTTCAGTGACATTTTGTGACTCAGTTCATTATCT1000.1466383165921255No Hit
GTACAGGGGATTCTACTCCCCAAAGGAAGTCTCACCATAGGCCTGTGTAGAC980.143705550260283No Hit
TACTACGGGGGGTTCAGGAGATGGCTCTGAGGAGCAGTTCAGGTTGGGAGGG970.14223916709436177No Hit
CCAGCCTGGGGGACAGAGCGGGACTGTCTCAAAAACAAACAAACAAACAAAC960.1407727839284405No Hit
TGAAATGGGGGTGCGCGCCGGCCGCGGCTTCAGCCTGGAGGAGCTCAGGGTG960.1407727839284405No Hit
CAAAATGGGAGGGGAGATAGTCACCACCATTCCTACCATTGGTTTTAATGTG960.1407727839284405No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC950.13930640076251924No Hit
AGCTAAGGGAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATA950.13930640076251924No Hit
TCTTTGGGGGGATTTTGTCTTTTTAAAAGATTATCTATGAATGCCAGTTTTG940.137840017596598No Hit
AACCGAGGGGGAAAATAAAGAAATATCCCTTGGCCAGGTGACAAAAATATCT940.137840017596598No Hit
GTGAGGGGTAAATTTTACTTTAGTAAAGTAAAACAAATTTTCTCTTATTTTT940.137840017596598No Hit
CGTGCCGGGATGATGGGGCCTGCTGTTCCCTGAGCATTCCTTTCATTCCTCA940.137840017596598No Hit
ATTGCGGGGAGGAGATCTGCAGAAGAGGGGCCTGACTGTTAGAAGGAAGACT930.13637363443067674No Hit
TTGCAAGGGCAGTCACAGGAAAAGAGTGGAAAAGTAGTAGTATTTGAAATGG920.13490725126475547No Hit
CCTTTCGGGACCAAACTTAAAACCAAACACTCATTTAAAAGTAGGTTAAGTG920.13490725126475547No Hit
GAAAAAGGGCTTTATTTCCATCTATGGTGTTGCAAATGACTGGATTTTATTT920.13490725126475547No Hit
TAAGTGGGGAGAGAAAAGCTTAAGAAAAAAATCTCTGTATTCAAATATGGAA910.13344086809883424No Hit
AGTTGTGGGATATTTTTATCTTTGTATAATTTGGTTTGGCTTTTCCTTGCCA900.13197448493291297No Hit
GCCCCAGGGATTTCCAAAAGTTGTAAACTTTGATGGCTGATTTTTCGTAAGT900.13197448493291297No Hit
TACAAAGGGTGAGATGGTCAGCCGGGTCATGAAAGCACTGGTAAACCGCAAG900.13197448493291297No Hit
GATATGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT890.13050810176699174No Hit
GCACCAGGGACTGCCCGCAGCCCTGGTTCCCACCCACGCCTCTCCCTCCACA890.13050810176699174No Hit
ACGCTTGGGACTCCAATTACTTTTCCATTCTGCACAATGATTTCTTCAATGG890.13050810176699174No Hit
TGATGAGGGTATCATCCATCTTTTAGAATCCCAGGGAGCTAATTTCTGGTCC890.13050810176699174No Hit
GCATTGGGAACTTATTGAAATTAACAGAAAATTTCTATTAGTAAAATAGAAT880.12904171860107044No Hit
TGGTGGCGGACTCCATTTCTCTCAAAAGCCCTCAAGTCACAAAGTAAATGGT880.12904171860107044No Hit
GCATCTGGGGACCCTTGACTTCTGATCTTTGTCTACTTCATTTAGCTGAGCA880.12904171860107044No Hit
ATCTGTGGGGAATTCATATTTTTTCTTGGACAATATGCTAATATTTATTGAT880.12904171860107044No Hit
TCTATTGGGATCTTAGCGCGGGGCCTCAAACGATGAATTGTAGAATGGAGCA880.12904171860107044No Hit
GCGTGAGGGAGGGTCAGAGGCAGGGACAGATGTGAGTATGCTATGCTGCTGG870.1275753354351492No Hit
ACTCACGGGACTCTTAGGGGCCATGGTCAAGTTCAATAAAATAAAAGATTCC870.1275753354351492No Hit
TGGCAGGGATTTCTGCAGGTAGAATAAATATCCTATTTAAAGCTTTCAAATC870.1275753354351492No Hit
CAGAAAGGGAGAGATGATTGAATTTTATTATTTTTCCCCAAATCTTTTCCAG870.1275753354351492No Hit
AACACCGGGAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTA870.1275753354351492No Hit
TAAAATGGGACAGGCTGTTATTTTTCTCAATACGAGGCGCAAGGTGGACTGG870.1275753354351492No Hit
AACAATTGGGGATATGGCAGGGCAGATATTCAAAGGGAGTTACAAAAGGACA870.1275753354351492No Hit
AAGACTGGGACTTGGACAGAGGGATTTGTGTGGATTGCAAATTAAGTCTTCA870.1275753354351492No Hit
TAGGTCCGGGACTATGAAATTATCTAAAAAATGCTCATAGGGTTCCTAATGT860.12610895226922794No Hit
ATTTCTGGGAATGCTACTTAATTGCAGTTTTTTCCCCAACAAGTTAATAGTT860.12610895226922794No Hit
AAGAAAGGGAATTCATGTGATAGCTAATATATTTGGTTTTAAATGCATCTTA850.12464256910330669No Hit
ATTTTCGGGGTTTTGCATATGACTGCTGCTACAAATTAAAACCCAAATAGAC830.12170980277146418No Hit
GTAAACGGGGGAGCAGAAAGGATCACAGATGTGGCTTTGGATTTCTGGAGGA830.12170980277146418No Hit
TGATGATGGGACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCA830.12170980277146418No Hit
ATTGGTGGGAGTGCCAGCCTCTGTGGCAGTCCCAATGCCAATCCCGTGGAGC830.12170980277146418No Hit
GTAGGAGGGATTGAAGGTGTAACTTGATAAAACTGTACCTGCGTTGCAGTTT830.12170980277146418No Hit
CTCAAAACGGGATTAACCAAAATAACAATGATAATAGTTCATTTTAAAAGTT820.12024341960554294No Hit
CTAATGCGGGATGCTAGTTTAACCCTACTATTAAAGCCTTACCTTTTGGTGT820.12024341960554294No Hit
TAGCCTGGGACCTGGCATAACATTCTCTCTATTCCACATTAAATGCACAGTA820.12024341960554294No Hit
GACGTTGGGGTAACAGATCAATAGTCCAAAAAAGTGTTTAGTAAATTAGGTT820.12024341960554294No Hit
ACTCCAGGGATTTTTACTAAGTTTAGTCTTAGAGATTTGACCATCCACCAAA810.11877703643962166No Hit
TGTACAGGGATCTCCTTTCTAGGCTTTCTACTTTCAGGCAAAAGCTGTTGAT810.11877703643962166No Hit
GCTATTGGGAGCCTCAGTTTCTCAACGATGTCTTTTGTTTACAGTGCTACAC810.11877703643962166No Hit
AACACCGGGATGCCGATCCAACTTAATTGCCAGGCAACTCTTCTGCACTTTA810.11877703643962166No Hit
TATAAGGGGACTAGTCCAATGTTACAAAATAATTGGAAAAAAGATGACAGAC810.11877703643962166No Hit
GCAGCCGGGAAGGAATATATTGACATCTGGGAAGAGTTAGATCCACCCTTGT810.11877703643962166No Hit
GAGGGGGAGATCCGCTGCTGCTGAGGAGAGGAGCGTCAACAGCAGCACCATG810.11877703643962166No Hit
AAACTAGGGGACTGATTAAAAATGCTAAACTCCTATATAGTAAAAAATAACA810.11877703643962166No Hit
ATACTTGGGAGTGTTTAGAGGGAAATTTATAGCACTAAATGCCCATAGGAGA800.11731065327370042No Hit
TAACTCGGGAAGTTGCAAAAGTTGGGGCTGCAATTAATGCTAACATAAGAGC800.11731065327370042No Hit
AAAATGGGACTGTGGGTTTCAGAGTAGAAATCCATAAATCAGTCCCACCGTT800.11731065327370042No Hit
AGAAGTGGGATGCCACCACAATACATTTTAATCTCACGTTTCAAAATACAAA800.11731065327370042No Hit
GTAACTGGGTCTGAGCAAATTAATGGGTACCTTTTTTTTCTTATTTAAAGCA800.11731065327370042No Hit
AAGTGAGGGAGAGGCAGGGTAGCTTGGATAGGAGACTCAGTGAGATTTTGTG800.11731065327370042No Hit
AACGTTGGGAGGTGGTAAGTTTTCTTCATACAGTTCTTTTGTGTGACAAACT800.11731065327370042No Hit
TTAGACCGGGGAGGGTGGGCTGCTGGAAGGCGCGACTGCAACTTGACTTTGC790.11584427010777917No Hit
ACTATAGGGATGGGCGGGATGGCCTATTTCATAGAAATGAAAATTTATGTGT790.11584427010777917No Hit
AACACTGGGCTTTGCTCACTCTGAGCAACAATGCCAATTATTCATGCAATCA790.11584427010777917No Hit
GTTTGAGGGATACTGTGTGTACCATGACTGCAGACTAGAGTTATGCCAACCT790.11584427010777917No Hit
CTCCTGTGGGGCGTCTGCACAATGGCTAGCACATGTCAGCATTTGACAATTG790.11584427010777917No Hit
AGCTATGGGATCACTTGCTGAGATGGGAAAGAGTTTAGGGAAGGAGGAAATT780.11437788694185791No Hit
ATTGATGGGATCGGTCCTGTTAGGAGGGGAGAAAAAGTTCTTCCAAAGGCTG780.11437788694185791No Hit
CTGTACGGGCAACTTAAGGGGAAAAAAACTTTTAGAGCACTTACTGGCAACT780.11437788694185791No Hit
CAGCTTGGGATACATGCTCTTTACAGAGCGTTTTTAGCTTTATAAATGACTT780.11437788694185791No Hit
TACTAGGGAGGCAATTTAAAGGTTATGGAGCTCTAACTAGTACTGCAGTATA780.11437788694185791No Hit
AAAATGGGGATGGCTTAACTTTCTCTTATGTTTTCTTAGAATATGTGTAAAT780.11437788694185791No Hit
GGCTTTGGGAGCTCCCCCTGCTTTCTTGCCATCTCTTCTCTGAGTTATTTCT780.11437788694185791No Hit
GTTACAGGGGACTAAATAGTTTTTCATCATTCTTTGTATTTTATTTCTCTAG770.11291150377593666No Hit
TACTTTGGGATATTAAAAGATGAATTAAATAGACAGTCTTTGACAAGAATGA770.11291150377593666No Hit
TGTTACGGGATTTGATTTTTTTCTTTGTTAGATGGAAAATGGGGGAATTGCT770.11291150377593666No Hit
CCGTCAGGGGTGATACATTTACCAAAAAACCTTATTAAGGCAATAACTAAAA770.11291150377593666No Hit
ATGCTAGGGATAGCAACTAACAGGGAGAGCGTGGGTTGAGAGGGAAATCAAT770.11291150377593666No Hit
GACTGACGGGATGTGGATTATGAACAAATGAAAGTTTGCTGTGTGATTGCAG770.11291150377593666No Hit
GGCGCAGGGCTTGACATCTACGTTTAAGAAGGGAACGTGGAAGAGAAGGTGA750.10997873744409414No Hit
TTGACGGGGGTGTAAGGAGGCATTTTGTGTGTCTTTGCAATGTATACAATTG750.10997873744409414No Hit
GTCTACGGGAACTTTATAAAACTAAGAACAAGCTTATAATTCATTTTTAAGC750.10997873744409414No Hit
TTGGCTGGGATGGCGTGCCCCTCCTGTGTTCTGAATGCTTATAGAGTGCCCT750.10997873744409414No Hit
CTCTTTGGGAGATGGGGAAACTGAAGCTCAGAGAGGCGAAGTCCTTGACTTG750.10997873744409414No Hit
CTCATAGGGATCTCTGTCTATAAACTTTAGGAAAATATGTAAAATCTTGTTC740.10851235427817289No Hit
AGGGCTGGGATGTACAATTATTAAATGTCAACTAAAAATAAAAGGAAAAAAA740.10851235427817289No Hit
GTGCAAGGGGTATTTATCCCTGAACATGTTTTGTACCTTTTTTTTTTTTTTT740.10851235427817289No Hit
ATAGAGGGGCAAATTTTCCCTTGCATAATGCTCAGTTTTTAAAATGTATTGC740.10851235427817289No Hit
ACAACGGGGTGGCTTGAACTTGCCAGATGCAAATACCACAGACTCCAAGAAA740.10851235427817289No Hit
TACCTGGGGATGAAGAAAACCAAGAATGTTTTGTTGGGCTGTGTTGAACATT730.10704597111225163No Hit
TGCGAGGGATCCCCAAGCCAACATACAATGTGAAATGAAAGCCAGTGCGTGG730.10704597111225163No Hit
ACAGATGGGATTGTTACTTCTTTTATATATAATTACTTTTGCACATAAGCAT730.10704597111225163No Hit
CTGTTTGGGCGATCTAAGCAAGGGATCTTTTATATGTTTAGATGCACAAAAC730.10704597111225163No Hit
CGGCTTGGGAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACA730.10704597111225163No Hit
AGTTGCGGGTACCAAGACGTCTGCTTTTGTGACAGTCAGAAAACACACCTGG730.10704597111225163No Hit
TACTTAGGGGAGTACAGAGTAGTTATTTGAGAGCATGAATTCTGGAGCTGGA730.10704597111225163No Hit
GGCATTGGGATTTTCTCATCATTTAATGTGATGTAGCTGTGGACTTTTGGAA730.10704597111225163No Hit
ATGCCAGGGGTGTTCTAAATTGCTTAAGCCATACGTTCTCTTAAACAGGATG720.10557958794633038No Hit
TATTCAGGGAAGCAAAATTTTCCTAGGAGCTGTTTCATAGACAGAAGAGTAT720.10557958794633038No Hit
TGAGTAGGGGGATGGACAGCTTCGGCGGTGGGAAGGGAAGGGACATGCTGCA720.10557958794633038No Hit
ATTATTGGGATTGTTTTTTATTAATTCCATCTCAGAATTTTTGTTAAAAATG710.10411320478040911No Hit
GCTATAGGGGGTGGCTGTTTTTGTAAATGTACAAACTTAGCAAAAAAATCAC710.10411320478040911No Hit
TTGCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA710.10411320478040911No Hit
TAAAAGGGGATCCTCCAGGGGCCGAAGGCTTCTTCCATTTCCGAACGTTGTT710.10411320478040911No Hit
CGCTTGGGGGACAATGCAAGGTAAGTATATCAAAGGAAATCAACAGTTGTGC710.10411320478040911No Hit
ACAACCAGGGAACCGGAGATAGAAACTCACCATGAGCGTATCAACCTTTCCT700.10264682161448786No Hit
GTATAAGGGCTGGCAAACAGTGCATTTCATATGCAGTGGGCATAACAAGTTT700.10264682161448786No Hit
CAGTATGGGAGAGCAGGAGGCCGACGTTTTCTCCGCATTGGAAGAGCCCGCC700.10264682161448786No Hit
GCAACAGGGACCACATTTTTGTGAAAGTGGGCCAAAGTAAAGTTTTAATTAT700.10264682161448786No Hit
CTTACAGGGGAACTTAGGAGAAAAGCCTGGGTTGAAACAGAGATTCGGATAT700.10264682161448786No Hit
CTATTTGGGATCTCAAGGAACTGTTCAGAACAATTAGGCAAGAAAAAGAAAT700.10264682161448786No Hit
TATGCAGGGATAGAACTCAATCCTATGGACAAGTTTACTCTTTCTAATCTAA700.10264682161448786No Hit
TGAACGGGGACAGGACCTGTGGGAGGGACTATCAGAGATGCAAAAATTACTT700.10264682161448786No Hit
AAATTAGGGGCATTTGTTCTTTGTTTTCCTCTCCATTTTTTAACCTTTTCTG700.10264682161448786No Hit
TCATCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC700.10264682161448786No Hit
GCTACTGAAGGGCAAAATGCTCTGTGATATATACATGATATGTTAATCATTA700.10264682161448786No Hit
GTGCTGGGGATGTACAGTTGAGCCTTGAACAAAGTGTGTTTGAACTGCCTGA700.10264682161448786No Hit
GGCATGGGGACTGTCTCCTAAACTTGTAAGAGGTCTGAAGCTATTCTTTGAT700.10264682161448786No Hit
AGTGCTGGGATGGTCATATTAGCAATTAGTTGCCTGGTATCTAAATAGGCAC690.1011804384485666No Hit
CGCTGGGGGATGCCAACAAGGACACTGACCACTCCAACTCTATCTGACAGGA690.1011804384485666No Hit
TAGTATGGGGATAATCTTAATTGATACAAAAAAGACATTTGACAATACCCAA690.1011804384485666No Hit
GGTTTTGGGGACGCTCTTCCGATCTGAGCCGGGGCTGAGGTCTGTGCTTGAA690.1011804384485666No Hit
GCTACAGGGAGCAGCTGCCAGTGATAATTTGTTTTACACTAACTTTTAAATC690.1011804384485666No Hit
AAAGTAGGGGGCGAAGGAAGTGCTGGAGTCGTGTGTTTTGGCTGCGCGTGAT690.1011804384485666No Hit
GGTTGTGGGGAGAATCCACAATACAGTAGTGTGGATAACTTTCAAGAAGCCA690.1011804384485666No Hit
TGGTAGCTGGGAATAAGGGAGAGATGAAAGTAGTCTGGATTGTTTTAGTGGT690.1011804384485666No Hit
CATCCAGGGATCGTGAAAATGGCCATACTGCCCAAGGTAATTTATAGCTTCA690.1011804384485666No Hit
GCTTAGCGGGATAGAGAGACTTCATCTCTACCCAAAATCAACAAAAAGCCAA690.1011804384485666No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT690.1011804384485666No Hit
CCGTAAGGGGGGAAATGACAGATGAGAGAGAATACAAATGTGGACGTAAATA690.1011804384485666No Hit
AAAACTGGGAGTGCTAAGTATGCCACTAAGTTTCAGATATATGGAATATTTT690.1011804384485666No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)