FastQCFastQC Report
Sat 31 Dec 2022
SRR5849792.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849792.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68085
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT6650.9767202761254314RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC5060.7431886612322832No Hit
TCTTTGGGGGGATTTTGTCTTTTTAAAAGATTATCTATGAATGCCAGTTTTG1250.1835940368656826No Hit
TGCTAGGGGAAGATGGAGAAATTTAGCAGAGAACTAGAAGCTAAAAAAAGAA1230.18065653227583167No Hit
TCTCGTGGGAGAGACATAATTATCAGAATTTAAAAATCTGAGTAACCAGATT1160.17037526621135346No Hit
GCCTCTGGGAGATGGTGGTAATTTTTATTCCCTTCTCTGCAATTTCAGCATA1120.1645002570316516No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1060.15568774326209883No Hit
AGTTGTGGGATATTTTTATCTTTGTATAATTTGGTTTGGCTTTTCCTTGCCA1040.1527502386722479No Hit
TACCTGGGGATGAAGAAAACCAAGAATGTTTTGTTGGGCTGTGTTGAACATT1020.149812734082397No Hit
ATTGATGGGATCGGTCCTGTTAGGAGGGGAGAAAAAGTTCTTCCAAAGGCTG1020.149812734082397No Hit
AACGAAGGGGGCCTGCGTAGCCGGCGGGCTGGCAGTGAGACTGACTGCGTCG1010.14834398178747155No Hit
GAAAAAGGGCTTTATTTCCATCTATGGTGTTGCAAATGACTGGATTTTATTT1010.14834398178747155No Hit
CAGAAAGGGAGAGATGATTGAATTTTATTATTTTTCCCCAAATCTTTTCCAG1000.14687522949254608No Hit
TACTACGGGGGGTTCAGGAGATGGCTCTGAGGAGCAGTTCAGGTTGGGAGGG990.14540647719762062No Hit
TAACTCGGGAAGTTGCAAAAGTTGGGGCTGCAATTAATGCTAACATAAGAGC980.14393772490269516No Hit
CAAAATGGGAGGGGAGATAGTCACCACCATTCCTACCATTGGTTTTAATGTG980.14393772490269516No Hit
CATTTAGGGGCCTTTCTCTGGTATCCTCTTGGCATGTGTAACATACATGTAG970.1424689726077697No Hit
ATCTGTGGGGAATTCATATTTTTTCTTGGACAATATGCTAATATTTATTGAT970.1424689726077697No Hit
ACGCTTGGGACTCCAATTACTTTTCCATTCTGCACAATGATTTCTTCAATGG970.1424689726077697No Hit
TACAAAGGGTGAGATGGTCAGCCGGGTCATGAAAGCACTGGTAAACCGCAAG960.14100022031284426No Hit
ATTGCGGGGAGGAGATCTGCAGAAGAGGGGCCTGACTGTTAGAAGGAAGACT950.1395314680179188No Hit
AGCGCGGGAAGAGGAAAGTCAGAAGAATCAAGCAAGACACATACCCAACCAA930.13659396342806787No Hit
TACATAGGGGAGAACACCGAGCACTGAACTTTGCAAAGACCTTCGTCTTTGA930.13659396342806787No Hit
CGTGCCGGGATGATGGGGCCTGCTGTTCCCTGAGCATTCCTTTCATTCCTCA930.13659396342806787No Hit
GCTCGAGGGATCTGCTCCTGCGTTTCACGCATCTGCATACCTTTGGTTACTC930.13659396342806787No Hit
GTGCAAGGGGTATTTATCCCTGAACATGTTTTGTACCTTTTTTTTTTTTTTT920.1351252111331424No Hit
GCCCCAGGGATTTCCAAAAGTTGTAAACTTTGATGGCTGATTTTTCGTAAGT920.1351252111331424No Hit
TGCAACTGGGCTGTAAACTGGAGTTAGCTCTAAATGCTTGATTATTTGGGTC910.13365645883821692No Hit
TAGGTCCGGGACTATGAAATTATCTAAAAAATGCTCATAGGGTTCCTAATGT910.13365645883821692No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT910.13365645883821692No Hit
CCGTCAGGGGTGATACATTTACCAAAAAACCTTATTAAGGCAATAACTAAAA900.13218770654329146No Hit
GTGAGGGGTAAATTTTACTTTAGTAAAGTAAAACAAATTTTCTCTTATTTTT890.130718954248366No Hit
AAACTAGGGGACTGATTAAAAATGCTAAACTCCTATATAGTAAAAAATAACA890.130718954248366No Hit
CTCATAGGGATCTCTGTCTATAAACTTTAGGAAAATATGTAAAATCTTGTTC880.12925020195344056No Hit
AGAAGTGGGATGCCACCACAATACATTTTAATCTCACGTTTCAAAATACAAA880.12925020195344056No Hit
ATAGTGGGGATGCAGAAAAAATTGAATCTTTATTGCTGATGGGAATATAAAA880.12925020195344056No Hit
TTTATGGGGTAGTACAGAATATCTTATTCAGATTACTTTTGAATAACCAGAA870.1277814496585151No Hit
GACATAGGGATAATAGTAGTAGGTTATTTCAACATTTTTTAATAGGTGAAGC870.1277814496585151No Hit
TTAGACCGGGGAGGGTGGGCTGCTGGAAGGCGCGACTGCAACTTGACTTTGC860.12631269736358963No Hit
GGTAAAGGGAAGGTAGATTGTTTGAAGCAGAAGTCAGTTCTCCTGGAGCTCA860.12631269736358963No Hit
GCTATAGGGGGTGGCTGTTTTTGTAAATGTACAAACTTAGCAAAAAAATCAC860.12631269736358963No Hit
AACACCGGGAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTA860.12631269736358963No Hit
TGATGATGGGACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCA850.12484394506866417No Hit
GTCTACGGGAACTTTATAAAACTAAGAACAAGCTTATAATTCATTTTTAAGC850.12484394506866417No Hit
CCAGCCTGGGGGACAGAGCGGGACTGTCTCAAAAACAAACAAACAAACAAAC850.12484394506866417No Hit
ACTAAGGGGAGCCTCCGCTGCCAGCGACATGTTCAAGGTAATTCAGAGGTCC850.12484394506866417No Hit
TGAGTAGGGGGATGGACAGCTTCGGCGGTGGGAAGGGAAGGGACATGCTGCA840.1233751927737387No Hit
TTACCTGGGACCGCCAGAATTGGCAAATACTATGAGTTGGGGCTGCACTTAC840.1233751927737387No Hit
AAAATGGGACTGTGGGTTTCAGAGTAGAAATCCATAAATCAGTCCCACCGTT840.1233751927737387No Hit
AACACCGGGATGCCGATCCAACTTAATTGCCAGGCAACTCTTCTGCACTTTA840.1233751927737387No Hit
GATATGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT830.12190644047881324No Hit
TTGTTTGGGAACAGCTAATGGAACCTTACTGAAAACCCATTTGCATTAATGC830.12190644047881324No Hit
GTTACAGGGGACTAAATAGTTTTTCATCATTCTTTGTATTTTATTTCTCTAG830.12190644047881324No Hit
CTCTTGCGGGTAAGAATAATTTGTCCCTGTCCCAACATCTCAGATAGAATAG820.12043768818388778No Hit
GCACCAGGGACTGCCCGCAGCCCTGGTTCCCACCCACGCCTCTCCCTCCACA820.12043768818388778No Hit
TGATGAGGGTATCATCCATCTTTTAGAATCCCAGGGAGCTAATTTCTGGTCC820.12043768818388778No Hit
TTGCAAGGGCAGTCACAGGAAAAGAGTGGAAAAGTAGTAGTATTTGAAATGG810.11896893588896232No Hit
TTGACGGGGGTGTAAGGAGGCATTTTGTGTGTCTTTGCAATGTATACAATTG810.11896893588896232No Hit
AAAATGGGGATGGCTTAACTTTCTCTTATGTTTTCTTAGAATATGTGTAAAT810.11896893588896232No Hit
CTGTTTGGGCGATCTAAGCAAGGGATCTTTTATATGTTTAGATGCACAAAAC810.11896893588896232No Hit
AAGACTGGGACTTGGACAGAGGGATTTGTGTGGATTGCAAATTAAGTCTTCA810.11896893588896232No Hit
GCATTGGGAACTTATTGAAATTAACAGAAAATTTCTATTAGTAAAATAGAAT800.11750018359403687No Hit
TCGCACGGGTCTCTGAATCGAGGCACTACCTCAATTCTCGGCTTAGTTATTT800.11750018359403687No Hit
AAGCTCGGGGGGATTTTCAGATCTTTTCATGAGAACTGGAAATGTAGCTGGG800.11750018359403687No Hit
AGCTAAGGGAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATA800.11750018359403687No Hit
AACAATTGGGGATATGGCAGGGCAGATATTCAAAGGGAGTTACAAAAGGACA800.11750018359403687No Hit
TATGCAGGGAGTATCAGACTAGCCTAATACTACGTATTTGTCATTACTTCAT800.11750018359403687No Hit
AACGTTGGGAGGTGGTAAGTTTTCTTCATACAGTTCTTTTGTGTGACAAACT800.11750018359403687No Hit
GCAACTTGTAAAATTGTACTCCCCAAGTTCAGTAGGATAGTAGAGAAGTCGT790.1160314312991114No Hit
TATCGAGGGACTGAAGGCAAATCTCCTTTGTCCATTTACCTGGAAACCAGAA790.1160314312991114No Hit
ATTGGTGGGAGTGCCAGCCTCTGTGGCAGTCCCAATGCCAATCCCGTGGAGC790.1160314312991114No Hit
TATACTGGGGGGGGGACTGTTGCGTAGGCATGATTGGTTGGGAAATGTAAAA780.11456267900418594No Hit
ACTATAGGGATGGGCGGGATGGCCTATTTCATAGAAATGAAAATTTATGTGT780.11456267900418594No Hit
AACACTGGGCTTTGCTCACTCTGAGCAACAATGCCAATTATTCATGCAATCA780.11456267900418594No Hit
GTTTGAGGGATACTGTGTGTACCATGACTGCAGACTAGAGTTATGCCAACCT780.11456267900418594No Hit
TGAACGGGGACAGGACCTGTGGGAGGGACTATCAGAGATGCAAAAATTACTT780.11456267900418594No Hit
AACCGAGGGGGAAAATAAAGAAATATCCCTTGGCCAGGTGACAAAAATATCT770.11309392670926048No Hit
TGTCATGGGCGATCTAAAGACGGGGTGTATTATTGAATTTTAAGCAATATTT770.11309392670926048No Hit
TACTTAGGGGAGTACAGAGTAGTTATTTGAGAGCATGAATTCTGGAGCTGGA770.11309392670926048No Hit
GGCTTTGGGAGCTCCCCCTGCTTTCTTGCCATCTCTTCTCTGAGTTATTTCT770.11309392670926048No Hit
GCGTGAGGGAGGGTCAGAGGCAGGGACAGATGTGAGTATGCTATGCTGCTGG760.11162517441433502No Hit
GCATCTGGGGACCCTTGACTTCTGATCTTTGTCTACTTCATTTAGCTGAGCA760.11162517441433502No Hit
TAGCCTGGGACCTGGCATAACATTCTCTCTATTCCACATTAAATGCACAGTA760.11162517441433502No Hit
GCAGCCGGGAAGGAATATATTGACATCTGGGAAGAGTTAGATCCACCCTTGT760.11162517441433502No Hit
ACAACCAGGGAACCGGAGATAGAAACTCACCATGAGCGTATCAACCTTTCCT750.11015642211940956No Hit
TCGTAAGGGAGTCTATTTTCTGTCTTGTTAATATTGATTAATTTTTATTGAC750.11015642211940956No Hit
GCTATTGGGAGCCTCAGTTTCTCAACGATGTCTTTTGTTTACAGTGCTACAC750.11015642211940956No Hit
GACGTTGGGGTAACAGATCAATAGTCCAAAAAAGTGTTTAGTAAATTAGGTT750.11015642211940956No Hit
TCGAACGGGGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAG750.11015642211940956No Hit
ATCAATGGGGGAGTAAACCTTTTGTAATTTAGACTCTAAATGTTATAGAATG740.10868766982448409No Hit
GATGAGGGGGAGGCTGTACAAGTGGTACAGTTCATATAGTTTAGTAAGAGCA740.10868766982448409No Hit
CCTTTCGGGACCAAACTTAAAACCAAACACTCATTTAAAAGTAGGTTAAGTG740.10868766982448409No Hit
GCAATCGGGAGAATGGGAGGTTAGAGAAAGATTAATGCGACGGACTTAAAAT740.10868766982448409No Hit
TATAAGGGGACTAGTCCAATGTTACAAAATAATTGGAAAAAAGATGACAGAC740.10868766982448409No Hit
GAGGGGGAGATCCGCTGCTGCTGAGGAGAGGAGCGTCAACAGCAGCACCATG740.10868766982448409No Hit
TATTCAGGGAAGCAAAATTTTCCTAGGAGCTGTTTCATAGACAGAAGAGTAT730.10721891752955863No Hit
CTTACAGGGAATTTGAAGGCGATCTTTTAAAAAGAGATTAAACCGAAGGTGA730.10721891752955863No Hit
GCTTAGCGGGATAGAGAGACTTCATCTCTACCCAAAATCAACAAAAAGCCAA730.10721891752955863No Hit
TCTATTGGGATCTTAGCGCGGGGCCTCAAACGATGAATTGTAGAATGGAGCA730.10721891752955863No Hit
CTAATGCGGGATGCTAGTTTAACCCTACTATTAAAGCCTTACCTTTTGGTGT720.10575016523463318No Hit
GGCTCGGGGATGGCTTACAACATCACACCCCTGCGCAGGGCCACAGTGGAAT720.10575016523463318No Hit
GCAAATGGGTGGATTCTAAAATTTTACAATGAATCATCCACAGTGCCACTAT720.10575016523463318No Hit
AGTCAGGGGGTGGCATTCCCTAAGGGCATCTAGGTGGCCATCAGACACCGAA720.10575016523463318No Hit
TGGATAGGGCAGAAATTGAGTCTTACTCATTTTAAATAGAATAAGCCATTTC720.10575016523463318No Hit
GAGATAGGGAACCTTTTACTTCAGTGACATTTTGTGACTCAGTTCATTATCT720.10575016523463318No Hit
GTAGGAGGGATTGAAGGTGTAACTTGATAAAACTGTACCTGCGTTGCAGTTT720.10575016523463318No Hit
CGCTTGGGGGACAATGCAAGGTAAGTATATCAAAGGAAATCAACAGTTGTGC720.10575016523463318No Hit
GCACTGGGGAAGGTAAGTGATTATTTAGACCAATAACATTGAACAAATCAAT710.10428141293970772No Hit
TTTCAGGGGGTGATCTCATTGTTCAATTCCCACCTATGAGTGAGAATATGCG710.10428141293970772No Hit
TGTTACGGGATTTGATTTTTTTCTTTGTTAGATGGAAAATGGGGGAATTGCT710.10428141293970772No Hit
TGCGAGGGATCCCCAAGCCAACATACAATGTGAAATGAAAGCCAGTGCGTGG710.10428141293970772No Hit
AAGAAAGGGAATTCATGTGATAGCTAATATATTTGGTTTTAAATGCATCTTA710.10428141293970772No Hit
TCTCTGGGGCGATCTTTGCATGGGATTCTGGGCTTTGAAATTCTTTTGTATA710.10428141293970772No Hit
TGGTAGCTGGGAATAAGGGAGAGATGAAAGTAGTCTGGATTGTTTTAGTGGT710.10428141293970772No Hit
TCAAGAGGGGTTTTCTGATTCTCTCTCACACAAATTTTCTTGTCAGTGGTAG710.10428141293970772No Hit
GTAACTGGGATGGAAGGAGGAATTCAAAGGAATCATGTCTTCAGTGTGTTTC710.10428141293970772No Hit
TAATAAGGGCGATCTATAACTGGGGACTTCTTTTACTTAACATTACTGTATG710.10428141293970772No Hit
CTCCTGTGGGGCGTCTGCACAATGGCTAGCACATGTCAGCATTTGACAATTG710.10428141293970772No Hit
GCTACTGAAGGGCAAAATGCTCTGTGATATATACATGATATGTTAATCATTA710.10428141293970772No Hit
AAAACTGGGAGTGCTAAGTATGCCACTAAGTTTCAGATATATGGAATATTTT710.10428141293970772No Hit
ATTTTCGGGGTTTTGCATATGACTGCTGCTACAAATTAAAACCCAAATAGAC700.10281266064478226No Hit
GTAAACGGGGGAGCAGAAAGGATCACAGATGTGGCTTTGGATTTCTGGAGGA700.10281266064478226No Hit
TAGTATGGGGATAATCTTAATTGATACAAAAAAGACATTTGACAATACCCAA700.10281266064478226No Hit
GGTTTTGGGGACGCTCTTCCGATCTGAGCCGGGGCTGAGGTCTGTGCTTGAA700.10281266064478226No Hit
TCCAGCGGGAGATGAGAATTTTCCGTATCTTGATCTGAGTGGCAAATTGTAA700.10281266064478226No Hit
TCGCGTGAGACAGATCAAGATCAAGACCGGCGTGGTGAAGCGGTTGGTCAAA700.10281266064478226No Hit
TGTCTTGGGTCTTGCATAGGGGAACTTTCAAATAGAAACAAAACAGTATGTA700.10281266064478226No Hit
ACTCCAGGGATTTTTACTAAGTTTAGTCTTAGAGATTTGACCATCCACCAAA700.10281266064478226No Hit
ACTTCTGGGATATAACATTATTTATATATATAACATTATAACCCATTGAAGA700.10281266064478226No Hit
AAATTAGGGGCATTTGTTCTTTGTTTTCCTCTCCATTTTTTAACCTTTTCTG700.10281266064478226No Hit
CCAACTGGGAGGGCATATAAAAGACTTATTTTATCCTAAATGTATTGGGAAA700.10281266064478226No Hit
TCATCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC700.10281266064478226No Hit
AATTATGGGGTTTCTCTTCGGTTTGAATTCTCTCATGGTTACCAAAACTCTG690.10134390834985679No Hit
ATTATTGGGATTGTTTTTTATTAATTCCATCTCAGAATTTTTGTTAAAAATG690.10134390834985679No Hit
AGCGATGGGGTTTCACTCCTTTCCATTTCTTTCTAAAATTACAGTAAAATAT690.10134390834985679No Hit
CTTATATGGGCTAAGACCTCTGATCATTAAAACCTATAAAGTGTTGAGCTGT690.10134390834985679No Hit
ACTCACGGGACTCTTAGGGGCCATGGTCAAGTTCAATAAAATAAAAGATTCC690.10134390834985679No Hit
ATAGAGGGGCAAATTTTCCCTTGCATAATGCTCAGTTTTTAAAATGTATTGC690.10134390834985679No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)