FastQCFastQC Report
Sat 31 Dec 2022
SRR5849788.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849788.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85146
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT7830.9195969276301881RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC5750.6753106428957321No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1680.19730815305475302No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT1150.1350621285791464No Hit
GTTGCCGGGGGACCACCATGGTAAAGAAGCGGAAAGGCCGCGTCGTGATCGA1130.13271322199516125No Hit
TGGTTGGAGAGAGGTTTAGACATAAATTTATTTGGATAAATAATAAATGTAT1100.1291898621191835No Hit
CACCAGCGGAGGCAAAAGAAAGAAATAAAGTTCATCCAAATTGAAAAGGAAG1100.1291898621191835No Hit
CGTCTTGGGTCCATTTGTCTAAGAGGCATAAAGCTGGAATTTACTGAGAGCA990.11627087590726516No Hit
TACCTAGGGAACAACAAAGAGTTTTATTTAATGAATCAATCATTAAAATAAA940.11039860944730229No Hit
AGACGTGGGATTTAAAATTAGGTGGTTTTTTTCAAGCACGAGTCACCAATGC930.10922415615530971No Hit
CTACAGGGCTGGGAGGTACCTGAAACCACAAAGCTCCTCCAAAACTAGCTTC910.10687524957132455No Hit
TCGGTGCGGGAACACAAAAGCATTTTTTTCTTATTTCTCATACTTTTGAATC910.10687524957132455No Hit
ATGTCTGGGGTAAAAGGCGAGCAAAGAAAAGTCAGCACTACTGGGTAAGCAG900.10570079627933197No Hit
TACGGAGGGAGTCCTTTGTTGGATGCATAGTTTGTAAACATTTTCTCCCTCT890.10452634298733939No Hit
CTCTGAGGGACTGCACAAACCAACTCGAAGTGATAATTAAAAGTGTGAGCTT880.10335188969534681No Hit
ATAGCGGGATGACACCAAAAGTAACAGATAAAAAAAACAGAATTAGAATTGG880.10335188969534681No Hit
ATCATAGGGGAACTCTTGCATGGGTTCCTTGAATCCTTATGTACATCAAAAA880.10335188969534681No Hit
GCGTAAGGGATATCAGCAAAATAATTCAATTTTGCAGGTTGCAAACAAATAT870.10217743640335425No Hit
ATTACAGGGACAGTGACAAGTACAAATAGTGTAAACACGTTTGAGAACAAAC870.10217743640335425No Hit
CGATTTGGGAAGGCTGAATTGTATGTACCACATTTTGTTTATCTATTCATCT870.10217743640335425No Hit
GGCCACGGGAACAAAAGGTAGAAAACAAAAGAAATACCATCAGAGAGAGGGA870.10217743640335425No Hit
TAAAGCGGGGGCTCAAATTCTCTACCTCTTCAGGGCTAATACTTTTAACTGA870.10217743640335425No Hit
TAATCAGGGATTGTCTACTGCCTACTGCTGAGATATTTTTTGAAAGTATTTT870.10217743640335425No Hit
GAATTGGGGGATATAAAGAGTCAAGATTCAGGTGTGCTGTATTCAGGGTTCC860.10100298311136167No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)