FastQCFastQC Report
Sat 31 Dec 2022
SRR5849787.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849787.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84386
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT8471.003720996373806RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC5920.7015381698385988No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1300.15405398999834094No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT1220.14457374445998153No Hit
TTATCAGGGATTCATAGATGCGCTTATTCATGTATTCACTCAATCAATATTT1140.13509349892162206No Hit
TTGCCGGAACTGAAGAAGTTTCTGGATACTGCCTTCAATCTCCAGGCCTTTG1080.1279833147678525No Hit
TTGCGTGGGAACCCAAGGATCCTCTGTTACAAAGATAACTTTTGAGGGGCGC1030.12205816130637784No Hit
GTTGCCGGGGGACCACCATGGTAAAGAAGCGGAAAGGCCGCGTCGTGATCGA1010.11968809992178797No Hit
AGACGTGGGATTTAAAATTAGGTGGTTTTTTTCAAGCACGAGTCACCAATGC990.1173180385371981No Hit
CGTCTTGGGTCCATTTGTCTAAGAGGCATAAAGCTGGAATTTACTGAGAGCA980.11613300784490319No Hit
TACCTAGGGAACAACAAAGAGTTTTATTTAATGAATCAATCATTAAAATAAA970.11494797715260825No Hit
TAATCAGGGATTGTCTACTGCCTACTGCTGAGATATTTTTTGAAAGTATTTT970.11494797715260825No Hit
TGAACAGGGAACTACATGGGTAAGAAAAAAAGAAACAAGACTCATTAACTAA970.11494797715260825No Hit
GAATTGGGGGATATAAAGAGTCAAGATTCAGGTGTGCTGTATTCAGGGTTCC950.1125779157680184No Hit
CTTAATGGGAGCACAAATGGGTGTGCCACACCTTTAACAAGAAGCCTGTGAA950.1125779157680184No Hit
GTTCCGGGAGTTTTCCCATGGAAGCACTTCAGGCAGTCCTGCCGAAGGTGCT950.1125779157680184No Hit
CTACAGGGCTGGGAGGTACCTGAAACCACAAAGCTCCTCCAAAACTAGCTTC940.11139288507572345No Hit
TCGGTGCGGGAACACAAAAGCATTTTTTTCTTATTTCTCATACTTTTGAATC940.11139288507572345No Hit
ACTACTGGGATTACAAAGCCGATGCTGCTGAGAAAATCAGAAAAGAAAATCC930.11020785438342853No Hit
TTATGCGGGAATTTAGAAACATTTTTGTATATATATGACAAATGAACTGTTT930.11020785438342853No Hit
CGATTTGGGAAGGCTGAATTGTATGTACCACATTTTGTTTATCTATTCATCT930.11020785438342853No Hit
CACAAAGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT920.1090228236911336No Hit
TGGTTGGAGAGAGGTTTAGACATAAATTTATTTGGATAAATAATAAATGTAT910.10783779299883868No Hit
CCGGGGTGAAAATAGTGTGACCTAATAGTGGTTGGAATGAATCTCCAAGAGG910.10783779299883868No Hit
GGTTCCGGGGTATTTTAAGAGAAGAATTTTTAGCCTAAAGCTTGATTTTACA900.10665276230654375No Hit
CACATAGGGGACAAAGGGCCGGAAGGAAGCCGGGGAGACAGGTGGGGTACTC890.1054677316142488No Hit
TTAAAGGGGCATGGTTACTTTATTATCCCTGCAACAGAGCTTCAACACTGCT890.1054677316142488No Hit
GCGTATGGGGTGACATTTACAGCTCTTAAAAATAAGGTCAGTTAAAATTGGA890.1054677316142488No Hit
TGTGCTGGGTCTTTGTACACAAAAGGTTCTAATTTTGGTGAAGTCTAATGTG880.10428270092195388No Hit
TAACGTGGGAGAACTAGAAAAATCTATTTCAAAATTCATATGGAACCAAAAA880.10428270092195388No Hit
GACTCTGGGCTGGTCGCGGGATGTTTTAGTTTCCTTGATAACATTTGAGATA870.10309767022965895No Hit
CACCAGCGGAGGCAAAAGAAAGAAATAAAGTTCATCCAAATTGAAAAGGAAG860.10191263953736403No Hit
GCAGGCGGATACTTGTCCTATTCTGTAGTAGAAAATAAGCTGCATGAGGACA850.1007276088450691No Hit
ATCATAGGGGAACTCTTGCATGGGTTCCTTGAATCCTTATGTACATCAAAAA850.1007276088450691No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)