FastQCFastQC Report
Sat 31 Dec 2022
SRR5849786.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849786.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85460
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT8190.9583430844839692RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC5780.6763398080973555No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1290.15094781184179734No Hit
GAATTGGGGGATATAAAGAGTCAAGATTCAGGTGTGCTGTATTCAGGGTTCC1110.1298853264685233No Hit
TAGTAAGGGATCCCCAAATTCTTCGTAGACAATTACAAATGTCCTTCCTCCT1050.12286449801076527No Hit
TGAACAGGGAACTACATGGGTAAGAAAAAAAGAAACAAGACTCATTAACTAA1020.11935408378188625No Hit
GTTGCCGGGGGACCACCATGGTAAAGAAGCGGAAAGGCCGCGTCGTGATCGA1010.11818394570559326No Hit
ACTACTGGGATTACAAAGCCGATGCTGCTGAGAAAATCAGAAAAGAAAATCC980.11467353147671426No Hit
TTCTTGGGAGAAAAAAATCCATCAACTCAGCCCCGTTAGCAAAACTGTTTGG970.11350339340042125No Hit
AGACGTGGGATTTAAAATTAGGTGGTTTTTTTCAAGCACGAGTCACCAATGC970.11350339340042125No Hit
CACCAGCGGAGGCAAAAGAAAGAAATAAAGTTCATCCAAATTGAAAAGGAAG970.11350339340042125No Hit
CGTCTTGGGTCCATTTGTCTAAGAGGCATAAAGCTGGAATTTACTGAGAGCA960.11233325532412826No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT940.10999297917154223No Hit
CGACAAGGGATACAGGGTTCCCTTTTCTCCACATCCTTGCAAAACACTTCTT930.10882284109524924No Hit
GTTCCGGGAGTTTTCCCATGGAAGCACTTCAGGCAGTCCTGCCGAAGGTGCT920.10765270301895623No Hit
TGTATCGGGCACTATGTCTCTAAATTGATCTTTTTAATTAAATCAGTTTTTA910.10648256494266324No Hit
TTATCAGGGATTCATAGATGCGCTTATTCATGTATTCACTCAATCAATATTT910.10648256494266324No Hit
ACACATGGGAACTACAAATAGAGATGAAAACAAGTACCTTTTATAACAACGA900.10531242686637023No Hit
ATGTCAGGGATTCCCCACTGAGCTCCACCTCCTGTCAGATCAGTGGAGGCGT880.10297215071378422No Hit
TGGTTGGAGAGAGGTTTAGACATAAATTTATTTGGATAAATAATAAATGTAT880.10297215071378422No Hit
TAATCAGGGATTGTCTACTGCCTACTGCTGAGATATTTTTTGAAAGTATTTT880.10297215071378422No Hit
ATGTCTGGGGTAAAAGGCGAGCAAAGAAAAGTCAGCACTACTGGGTAAGCAG880.10297215071378422No Hit
TCTACTGGGATGTGTTTCTGTAAAAGCTTGTACAATATATTATTGATATGTC870.10180201263749122No Hit
CTTATGGGTAGCAATATGTGATGGAAAAGTGGCATGCTTTGGGCCACATTAG870.10180201263749122No Hit
ATATAGAGGGAGGGTGGGGCTTAGAATGGGGCTTCCATGGTGGTTTCAGAAA870.10180201263749122No Hit
GTCTCTGGGCCGATCTTGTCGCGGGAATTCAATTATTGTGGAAGACAGTGTG870.10180201263749122No Hit
TACTATGGGATTTTCTGAATACAGTGGCTTACTAGTTTCTTTTGAAGATTTT860.10063187456119822No Hit
GGCCACGGGAACAAAAGGTAGAAAACAAAAGAAATACCATCAGAGAGAGGGA860.10063187456119822No Hit
GGTTCCGGGGTATTTTAAGAGAAGAATTTTTAGCCTAAAGCTTGATTTTACA860.10063187456119822No Hit
TTCATTGGGGACTCTCTTCTGCTTTGACCTGCTTCCAGACACACCATCAGCA860.10063187456119822No Hit
CGTTACGGGATTCTTTCTATTTGTCAGGTGCACAAGATTACCTTCCTGTTTT860.10063187456119822No Hit
GACTCTGGGCTGGTCGCGGGATGTTTTAGTTTCCTTGATAACATTTGAGATA860.10063187456119822No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)