FastQCFastQC Report
Sat 31 Dec 2022
SRR5849785.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849785.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39884
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT5041.2636646274195167RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC3840.9627920970815363No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC900.2256543977534851No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACA680.17049443385818874No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGT650.16297262059973924No Hit
GCTCCTGGGTGACAGAGCAAGACTCCGTCTCAAAAAACAAAAACAAAAAAAA570.14291445191054056No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT520.1303780964797914No Hit
TCACCTGGGAATCTTTGGTAGTACACCATAATAGATGGGAAGACTAATAAAT410.1027981145321432No Hit
CAACTTGGGAAGACCTGGAATCATTTTTCTGATAATGAGGCAGAGCGGGTTA410.1027981145321432No Hit
TGTCCGGGGACCCAAGGTCACCTAAATTTCCTCCTATGTTATCTTCTAGGAT400.10029084344599339No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAAACTCGTATGC400.10029084344599339No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)