FastQCFastQC Report
Sat 31 Dec 2022
SRR5849784.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849784.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39766
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT5281.327767439521199RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC3440.8650606045365387No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1150.2891917718654127No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT610.15339737464165368No Hit
ATAACTGGGGATTCCCTCCCTGACCCAACCAATCAGCGTTCACCATTCCTTA460.11567670874616508No Hit
GCAATTCGGGAGAATGATTATGCTTTTCATAAGCTTAAAAAAAAGCCTTTTT460.11567670874616508No Hit
TAGAGGGGGAATTCCTTCAAGATGGCATGCCCTTGGTGGCACAGCTGGATCA430.10813257556706733No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACA420.1056178645073681No Hit
GTTGGCGGGCTGTTTGAAAATCCTACCAAAATTGGGGAGTCCATTCCAAGAT410.10310315344766885No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGT410.10310315344766885No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)