FastQCFastQC Report
Sat 31 Dec 2022
SRR5849783.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849783.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39952
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT5131.2840408490188226RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC3420.8560272326792151No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC960.24028834601521826No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT610.1526832198638366No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACA550.13766519823788548No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGT540.13516219463356027No Hit
TAGAGGGGGAATTCCTTCAAGATGGCATGCCCTTGGTGGCACAGCTGGATCA430.10762915498598317No Hit
CAAACAGGGAAACAATGAGAGATTGTGTGGAATGAAAAGCACTATTGCTACA420.105126151381658No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAAACTCGTATGC420.105126151381658No Hit
TCACCTGGGAATCTTTGGTAGTACACCATAATAGATGGGAAGACTAATAAAT410.1026231477773328No Hit
CGCGCAGGGAGCACTAACTGATTCACTAACAAAAAAAATTAAACTTCTAACT400.1001201441730076No Hit
ACATTGAGGGAGCTCAAAGTTCAAGGAAATTAGATTCCCCATGCTGCACCTA400.1001201441730076No Hit
GCAATTCGGGAGAATGATTATGCTTTTCATAAGCTTAAAAAAAAGCCTTTTT400.1001201441730076No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)