FastQCFastQC Report
Sat 31 Dec 2022
SRR5849782.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849782.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61766
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT8801.4247320532331704RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC6591.066930026228022No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1340.21694783537868728No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT1010.16352038338244343No Hit
GCCAAAGGGGGCGAGGATTGGCTGTTAGCGGCGTTGTAGTTAAGCTCGTGTA980.1586633422918758No Hit
AATGCAGGGAGAAGAAAACAAGTCACTTTTCAGTTCCAAAATATTCAATAAC960.15542531489816405No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACA840.13599715053589354No Hit
AGTCAAGGGGGCCGGCCGCGATGAGCGGGGAGCCGGGGCAGACGTCCGTAGC780.12628306835475828No Hit
ACTGCGGGGAGGTAAAGGTACATATCTCCTGACATGAGCTATTTGTTAACAT760.12304504096104653No Hit
GAGATAGGGCGCGGTGGGAGGGATCAGAAAAGAAAGTCATCAAAAGAGATCA720.11656898617362302No Hit
AATTATGGTCTTCAGGAACTGCGCAAGGAGGAGGAGACGCTGCTGCGGTTGA710.11494997247676715No Hit
AAGTTGGGGGGATTATATGGACTTGAAGCCACTTTGATCCCTGTTTTATCGT700.11333095877991128No Hit
TATTAGGGGAAGTCTGCATGTTTCAATTATAAAAGTGTAGTATATGCTTATA700.11333095877991128No Hit
TTCGAAGGGACATTAAACAAGTAGAAAAACTTCAAATAAACAACCAGTGATT700.11333095877991128No Hit
TTGCGTGGGGAAATGATCAAACACGCTGGTAAAGCAAACACACAAGGAAGAG670.10847391768934364No Hit
CTCAAAGGGATTTCAACAAATAAACTGTTGACACCTGGCCCTTTTACTATTT670.10847391768934364No Hit
CATTACGGGAGTTTACAAAAAAGTCAGCTCTTGACCAATTATTAGAAATAGA660.10685490399248776No Hit
AACTTGGGGTGATTTTAATTTGCCTAATTATCTTATGACAAGTATCAAATTA640.10361687659877603No Hit
ACGGGGGGGAGTCTTCAGGCACTATTTTAACAAGTGGTTATTTCTTGATAAT640.10361687659877603No Hit
ACATAGGGAAGCGGAGGGAGTCGAGCCAGCGTCGCCGCGATGGTGTTGTTGG620.10037884920506426No Hit
CGATATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA620.10037884920506426No Hit
CATACAGGGGGTTTACATTCTGTTCTTCTCGATGCACTCACAAGCGGGTAAC620.10037884920506426No Hit
GAGATGGGAGGGAAGAAAAGACTGAAGGAGAAAGGAAAGAGGGAGTAAGGAA620.10037884920506426No Hit
TTGTTGGGGACTTCAATAAGGTAGAAGTATATTTTTCACTCATCTTCTAGAC620.10037884920506426No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)