FastQCFastQC Report
Sat 31 Dec 2022
SRR5849781.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849781.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61588
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT8881.441839319347925RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC6150.9985711502240696No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1420.23056439566149248No Hit
GCCAAAGGGGGCGAGGATTGGCTGTTAGCGGCGTTGTAGTTAAGCTCGTGTA1240.20133792297200753No Hit
AATGCAGGGAGAAGAAAACAAGTCACTTTTCAGTTCCAAAATATTCAATAAC880.1428849775930376No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT850.1380138988114568No Hit
TATTAGGGGAAGTCTGCATGTTTCAATTATAAAAGTGTAGTATATGCTTATA790.12827174124829513No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACA760.1234006624667143No Hit
TTGCGTGGGGAAATGATCAAACACGCTGGTAAAGCAAACACACAAGGAAGAG710.11528219783074624No Hit
GCTTCGGGGGGGCAGCGGGTGCCAGGCACGGTGTCAGCAGGCAACATGGCCG700.11365850490355264No Hit
CCCAGCGGGTTAACATACTGGAAGTATTTTTATAAATACTTTTTTTTTCTCT690.11203481197635903No Hit
AAGCTTGGGGATTTTTCTGTACCATATTGTTCTCTTACACATACGTATGTTA670.10878742612197181No Hit
AGTCAAGGGGGCCGGCCGCGATGAGCGGGGAGCCGGGGCAGACGTCCGTAGC670.10878742612197181No Hit
AGGATTGGGATAATGGAAGCATATAAGGATTGCATTTTCCCAGTCTGTAGGA670.10878742612197181No Hit
TCTGTAGGGTGGCATGGGGCAAAACAAAAAGAGAATGTCTTTTCCATGTGAG660.1071637331947782No Hit
AATTACGGGATACATATTATAGTCAAACTGCTAAAATTCAGTTATAGATAGA660.1071637331947782No Hit
CATTACGGGAGTTTACAAAAAAGTCAGCTCTTGACCAATTATTAGAAATAGA650.1055400402675846No Hit
ACACAAGGGCGATCTTATAACGGGAGACGTTTCAGGCCTACTTGGATGATTG650.1055400402675846No Hit
CTGATGGGACAACAGAATTATATCCTGTGGTTTGGGAGTTTAAAAGAGGAGT650.1055400402675846No Hit
GAGATGGGAGGGAAGAAAAGACTGAAGGAGAAAGGAAAGAGGGAGTAAGGAA640.10391634734039099No Hit
TCGACTGGGACTGAAACAAAGAGGAACATTTCAAATCATTTTGGAAGATTCC630.10229265441319738No Hit
CATACAGGGGGTTTACATTCTGTTCTTCTCGATGCACTCACAAGCGGGTAAC620.10066896148600377No Hit
CAGCGCGGGGAACTGAAAGCCGGAAGGGGCAAGACGGGTTCAGTTCGTCATG620.10066896148600377No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)