FastQCFastQC Report
Sat 31 Dec 2022
SRR5849780.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849780.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62996
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT8711.3826274684106927RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC6361.009587910343514No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1160.1841386754714585No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT910.1444536161026097No Hit
AATGCAGGGAGAAGAAAACAAGTCACTTTTCAGTTCCAAAATATTCAATAAC860.13651660422883993No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAAACTCGTATGC830.13175439710457806No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACA810.12857959235507016No Hit
ACTGCGGGGAGGTAAAGGTACATATCTCCTGACATGAGCTATTTGTTAACAT810.12857959235507016No Hit
GAGATAGGGCGCGGTGGGAGGGATCAGAAAAGAAAGTCATCAAAAGAGATCA780.1238173852308083No Hit
AAATATGGGGGAATTCTTTGACTACCTTAGTTTTTCTAGAGTTTCACTTAAA730.11588037335703853No Hit
TTCGAAGGGACATTAAACAAGTAGAAAAACTTCAAATAAACAACCAGTGATT730.11588037335703853No Hit
AGTCAAGGGGGCCGGCCGCGATGAGCGGGGAGCCGGGGCAGACGTCCGTAGC720.1142929709822846No Hit
GCCAAAGGGGGCGAGGATTGGCTGTTAGCGGCGTTGTAGTTAAGCTCGTGTA690.10953076385802274No Hit
CTCGCAGGGTCCTCTTTTCTAGAATGGTGTCATCTCCATTTTCTCTGCCTGA680.10794336148326879No Hit
TATTAGGGGAAGTCTGCATGTTTCAATTATAAAAGTGTAGTATATGCTTATA670.10635595910851482No Hit
CTGATGGGACAACAGAATTATATCCTGTGGTTTGGGAGTTTAAAAGAGGAGT660.10476855673376087No Hit
CCAAACGGGTAACAGGAGCAAATTGAGACCTCAACTGAATTCTGCCTTGAAG660.10476855673376087No Hit
TTGCGTGGGGAAATGATCAAACACGCTGGTAAAGCAAACACACAAGGAAGAG650.1031811543590069No Hit
AATTACGGGATACATATTATAGTCAAACTGCTAAAATTCAGTTATAGATAGA650.1031811543590069No Hit
CTCAAAGGGATTTCAACAAATAAACTGTTGACACCTGGCCCTTTTACTATTT640.10159375198425297No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)