FastQCFastQC Report
Sat 31 Dec 2022
SRR5849778.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849778.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94336
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT11871.2582683175033922RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC7460.7907903663500679No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1770.18762720488466755No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT1120.11872455902306649No Hit
AATGGCGGGACACTTAAGTTGATTTCATATCTTGATTATTGTGAATAGTGCC960.10176390773405698No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)