FastQCFastQC Report
Sat 31 Dec 2022
SRR5849777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94199
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGCCGTCT11211.1900338644783914RNA PCR Primer, Index 36 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCTCGTATGC7570.8036178728011976No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCTTATAC1600.16985318315481057No Hit
AATGGCGGGACACTTAAGTTGATTTCATATCTTGATTATTGTGAATAGTGCC1090.11571248102421469No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCTTAACAATCCTGTCTCT1090.11571248102421469No Hit
CATCGCGGGGGAATCTGATTTTCACGCTAAAGTTCCTGTTGTGGTGTGCAGA1070.11358931623477955No Hit
GATATGGGCAGTGAATGGACCCAGGGTTCCACCAAGAAATGGCTGATTACAG1050.11146615144534443No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)