FastQCFastQC Report
Sat 31 Dec 2022
SRR5849773.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849773.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64955
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC17152.6402894311446388No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT12031.8520514202139942No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA4280.6589177122623355No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC1880.289431144638596No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT1800.2771149257178046No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA1630.2509429605111231No Hit
ACTACAGGGACACTAGTGGTGGTGGAGATGGAAAGGGACAGATTCAAGACAG1490.22938957739973828No Hit
GTTTCTGGGACTCGCGTCTGGCTACCAGCTCCCCGCTGCCCTGAGCTCGGCG1400.21553383111384805No Hit
CTTTCCGGGGTTGGTGACTTGTTGACACGTGTTCCCATTTGATTTGGCTTCA1380.21245477638365023No Hit
AACGAGGAGTGTTCCTTGCATTTTGGGACAGAATGGAATCTCAGACCTTGTG1280.1970595027326611No Hit
TTCTATGGGATTGACCAATGGCCTCTGAAAAAGCACATTTTAGATACTGAAA1230.1893618659071665No Hit
ACATTTGGGACAGGAATTTTCTTGAAGATGGAGAAAGTGATGGCTTTTTAAG1210.18628281117696868No Hit
ACATCGGGGATGTTCTTCAAAGTTTTGGTAGCTAAATCGGAAAGTTCTATAG1190.18320375644677084No Hit
GCCACGGGGGGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTT1180.18166422908167193No Hit
TTTGGCTGGGAAGAGGATTCATGGCGAATATATGTGATAACATCTTTATACT1180.18166422908167193No Hit
AACTGTGGGGATTCTTGCCCTTTCCAGCAGCTGCAGCTGCCGTTTCTCTCTG1170.18012470171657302No Hit
GCATCTGGGTAACATCAAGCTTTAACAAATATTTAAAGACAAAAAAATCACG1170.18012470171657302No Hit
GCACATGGGATATTCCAAGAGCTCACACCATTTGCAATAATAATAAAACAAT1170.18012470171657302No Hit
CGCATCGGGGAAAGCCCTGGATCTCTTTGCACCAAAAATGGTCCAGATAGAC1150.17704564698637518No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1140.17550611962127627No Hit
GGTTTGGGGAACTATACAAAATATTGTTGTATTTATACAAAACAACAATACC1140.17550611962127627No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC1120.17242706489107845No Hit
ATTGGGGGTTACAGACTAACAGCATCTCAAGGCAGAAGAATTTTTCTTAGTA1120.17242706489107845No Hit
ACTGCAGGGGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGA1120.17242706489107845No Hit
AGGACTGGGATGTGATAGAAGTAGCATGTTGCTGCCAGAATACAAGCATTGC1100.1693480101608806No Hit
TTAAAAGGGAAGAGAGTGATTATTTGCATTACAAAGGAATTCTTAATAATTC1090.1678084827957817No Hit
GCACTGGGATCTAGACATTATGCATATAATGTCCAAAACATATGCATATACA1050.16165037333538604No Hit
ATCTTCGGGAGCAAGCACAATTCTGTACTTTGGTTATACCATGATAATGGTT1040.16011084597028713No Hit
ACTGCAGGGAACTTTAAAAACTCCTGTATTTTGTATGGTTAATTTGCTGTTA1020.1570317912400893No Hit
CTAGCAGGGGGATACTTGAGAAATAATTTTTGATTGTCTAATCAAAATGATA1020.1570317912400893No Hit
GAACATTGGGACGCTCTTCCGATCTATAATAGGGTGCGAGAGAAGGATAAGC1000.15395273650989147No Hit
CTTAATGGGCATTCTGAGGGAACACTAGATATAAATGGCGTAACTCCCAAAG990.15241320914479256No Hit
TGATATGGGATTGAAACTGGGCTGGAAAACTGCAGTCCCAGGAACTCCAGAG990.15241320914479256No Hit
TGCTCCGGGTAAGATACATATACACAAATATATAAGCTTTCCCCATGAAATA980.15087368177969365No Hit
ATATAGGGGTCGCTCATTGCATACTCTTCAATCAGCCACATAGCCCTCGTAG970.14933415441459472No Hit
GAAGGTGGGGGAGGAATACATTATTTTATCGTATATTTTTGTCCAAGCGATG960.1477946270494958No Hit
CTCTATGGGAAGAAAAACAACTCCTCTTTCCCATAGTCAGAGTTGAATTTGT940.144715572319298No Hit
TAGGTAGGGACGGGCGCCTGCCACCACACCCAGCTAATTTTTGTATTTTTTA940.144715572319298No Hit
ACATGAGGGATAACAAAACATCTGGGCTTTATAAGTATGTAAAGAATAATGA940.144715572319298No Hit
CATTCAGGGAATATTTTAATGGTATAGATCTGCTAATGAATTCTCTTAAAAA930.14317604495419906No Hit
TCGTTGGGGCTACAAAAGAAAGGAGAGTAGTATCATTACCCAGCTACAGTCA920.14163651758910015No Hit
ATAAATGGGATTGCTTCACTACCCATCGGTGTCCATTTATAGAATAATGTGG910.14009699022400124No Hit
GCTCACGGGGCTCTTCCACAGGAGGCCTACACGCCGCCGCTTGTGCTGCAGC910.14009699022400124No Hit
TGTAGCCGGGGTTTGGGAATGGTGCCCTTGGAGGCAACCTCCCATTTTAGAT900.1385574628589023No Hit
GGCGTCTCGGGAAATGCATGTTTGTTAGAATGGAAACAGAAAAACATTAAAA900.1385574628589023No Hit
CTATAGGGAGTGTTTTTATTACTTTCATCTGAAACATTCCATGTTTTAATCT900.1385574628589023No Hit
TGTTAACGGGATGCCACAGAACAAACACTACTCTAGCAGAGCCTTTCTTGCA900.1385574628589023No Hit
TAGCTTGGGACCATTAAGTTGTTTTTGAAAAGTCAACTCTGAGTTGTCCTGT890.1370179354938034No Hit
GCTAATGGGATTTAAATTTTATTTATATCAATCTTCTCTAGTGGGAGTAAGA890.1370179354938034No Hit
TAAAATGGGGAGGCCAAGGCCAGTGCGGTTTCCCTTCCACTGCCTCAGTTTA870.13393888076360555No Hit
ACGGCTAGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT860.13239935339850664No Hit
CGTAATGGGAAATTCTCGTAATATGCCATATGTTCACTTACCTCTCTGGGAT860.13239935339850664No Hit
AACTAAGGGGAGGTTAGCTGAGGCTGGAAATGCCTTTTTCATGGTAAATGAT860.13239935339850664No Hit
TGGCATGGGACACAGATAACTTTATGCTATTACATTCAGAGTTATTTCATTT860.13239935339850664No Hit
GCAATAGGGGAAGTGGCTTCAGGAGTATAAATTCAGCTAATTATTTCTATAT860.13239935339850664No Hit
GCCATGGGATCAATGGTTATCTCAAAATCGTCTTTTAGGAGATCCCCAATTG860.13239935339850664No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC850.13085982603340773No Hit
CTTGCTGGGGCATTTTCGTTGGTGGTGTTCAGTTGTGGCGGTTGCTGGTCAG840.12932029866830883No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT840.12932029866830883No Hit
TAGCGCGGAACTCATATTTTAACAGTTTTGTGAAAAAAATAGAAAATATTTT840.12932029866830883No Hit
TGTAATGGGAAATCCACTCTGCAGGAAATAGGCCCAACCACCAGATGAGAAT840.12932029866830883No Hit
CTCTACCAGGGATGTGAGTGAAGACCACCTGGATCTCCTGCCTCCTCCCTAG830.12778077130320992No Hit
TTGTCCGGGATTCAACAATTGTCAAAAGCCCACCACAGAACATTTTCTAAAT820.12624124393811098No Hit
CAAAGCGGGGCTACCTAAATTGAAATCCTTTTCAGAAAAAATATAATTGCAA820.12624124393811098No Hit
TATCGCGGGATCTCACAGAATTTTGATTTGCCTACTCACCCACAGGAGACAT820.12624124393811098No Hit
TCAGCGGGAAGAATTTCATCCTATGTAAATTTGTCCTAAACCTTTTTCTTTA820.12624124393811098No Hit
TAAGCTAGGGACTGTAAGCAAATTGCTTAGCTTCTCTAGACATCAGTTTCCA820.12624124393811098No Hit
TTACAGGGATATGGAAGTTCAGAAGAACTGGGCTAGCCAAAACAACTTTGAA820.12624124393811098No Hit
TGTTGTAGGGACTGAGAGAAGAACCACAGTTGAAACCTGCTTTTATCATTTT810.12470171657301209No Hit
TACGCGGGGAAGGAATGATAGAGCACAATTCCACCTGAAAATCATGTTATAA810.12470171657301209No Hit
AATGCCGGGGAACTTTGTATGAGTATACCTGACTAAAAACACTAATGCGGTT810.12470171657301209No Hit
AACAGAGGGTGCAGTTCTGGTACATAAAGATGAGTAATATAAACTGAAATCT800.12316218920791318No Hit
GTTGTCGGGGCGCGCTGGTGCAGCCACCCTCGCTCCCTCTGCTCTTCCTCCC800.12316218920791318No Hit
TATCTTGGGCTGCCAACACTGGTTTCCCAGATGAGCAAACCGGCTCTTCTGA800.12316218920791318No Hit
AAAGCGCGGGGTCCCAGGAAGAGCTTCCCCACTCCCATCCCCCAAATTGGAA800.12316218920791318No Hit
TGTAATGGGAGAATGGAAAGTTAAATATTTTTGCAGTGTGTATTTAAAAGAG800.12316218920791318No Hit
GCAACAGGGCTGCAAGCCAGTCAAAGTGGCACAGCAAATCATATAAATCGAA790.12162266184281426No Hit
ATAAGGGGACCCAAACTCAAAAAATATGAGTACTTGCGTACCTCCATTTCTG780.12008313447771535No Hit
TACCACGGGGGGCCACTGTTCAGGGTACTGACCATCAGTGTCAGCATTAGGG780.12008313447771535No Hit
GATACAGGGACTCAGACTCTGAGGCCTGGTGTGAAGCTTACACTCTCTGCTC780.12008313447771535No Hit
GTTTGAGGGGTACCTTGCACACATCATCTGTAAGCTGTTTGGTTTTAAAATA770.11854360711261643No Hit
TTCTAAGGGAAGACAACATGCATATATGACAATCATATATGATGTATTTGAT770.11854360711261643No Hit
TGCCTTGGGGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCA770.11854360711261643No Hit
CAGTGTGGGATAGTTCACTTCAAATAATGTAAGATGCAAGATGATTAACCTA770.11854360711261643No Hit
TCTACGTACGGGCAATAATTAATAGTTAAACAAACGATTACATTCATTTCAT770.11854360711261643No Hit
CACGAGGGACCAGCAGATTCTGCGAAAGCACCAAATTTCTCAAGACCCTCTT760.1170040797475175No Hit
GTACACGGGAAGCAGCTTACACTAGGCTTTTTTACAGCTGTGCTTGAGAACA760.1170040797475175No Hit
GATTTGGGGGAAAATCTCAAGTGGCTGAACTGAATGATGATGATAAAGATGA760.1170040797475175No Hit
ACAAGTGGGACATTTTGTGCAGTGATTATTTTTTAAAGTCTTCTTTCATGTA750.1154645523824186No Hit
TGCTAAGGGATTGACTATGGGGACTTTTGGGGCCCCCTTAAATTTTGCACTT750.1154645523824186No Hit
ACTTGGGGGTTTAAACAATCTTTCATGTCTTGTCATACAAATTTATAAAGGT750.1154645523824186No Hit
CAAAAGGGGAAAGTTAAAAAATTGTGCATGTCTGAGGGGTTGAATATATTTT750.1154645523824186No Hit
AAGAGGGGGGGTCTGCAGTCTGGAAGCTTGTCTGCTCTATAGAAATGAAAAT750.1154645523824186No Hit
GACAACGGGAAACTGATTCTAGGCAGAAAAAGACAACTTTGTTTTCTTTTTA750.1154645523824186No Hit
CGCTTTGGGGATAATCAGGCTCTGTTTGCACTTTATATTTTAGCAGATACAG740.11392502501731969No Hit
AATCTTGGGAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCC740.11392502501731969No Hit
TATGGGGGGAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTCGTAG740.11392502501731969No Hit
GTTATGGGATATTACCTAACTTCTAGTGTAGTACTGGTTCTAACAAGTAACA730.11238549765222076No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT730.11238549765222076No Hit
GCAATAGGGAGAGTGGATTAAGTTAATTTTATTTTGTCAGTGTTACTGTTTT730.11238549765222076No Hit
CTAGCTGGGGAGGTGCTTAAACTTTATAGAATACAGCTTAGCTCCCTATAAG730.11238549765222076No Hit
TGATAAGGGATAGGGAGGGCTCTGCATAGCACCTGCTATAGCGGTGTCATGT730.11238549765222076No Hit
CACAGGGGGAGCTATGGGTCAGGTTATTCTAAGCTTTTATATACTCATGCCA720.11084597028712184No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA700.10776691555692403No Hit
TATTATGGGATTGATATGAGTGAATATACTAAAAGAATTTATGTTCATCAAA700.10776691555692403No Hit
TGGTAATGGGACTACGTTGTAGCCCACTTCCACTATGTCCTATCAATAGGAG690.10622738819182512No Hit
TGCTGGGACCTGCTGCTGATGCAATGCAATGCATCCCAATGGTTGTGGGGAT690.10622738819182512No Hit
CATTCTGGGGGGGTCTGGGAAACAGAACCTTAATGTTACAGGTACAAAAGCA690.10622738819182512No Hit
ATGCTCGGGGCCTTCCTTCCTGTAGCTGTCGCTTTCTCAGGCTTGGACTGTC680.10468786082672618No Hit
AACTGGGGACCTCACCGGCTGCCTCCGCGTCGGAAGCGACGGTGTCAGGACA670.10314833346162727No Hit
GATTAGGGGATATATAAGAAAGACATCTTTCTCTTTTCTAAAAGACTTCCCT670.10314833346162727No Hit
TGATTAGGGGAGAGAGATTTCTGATATTGCCAGAAGACAAGTTGAAATTTTG670.10314833346162727No Hit
GTCTCTGGGATGGTAAAATGGTTTCTTTTGGCAGTTTACCTCATTATGTTTT660.10160880609652836No Hit
ATCTAAAGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTAC660.10160880609652836No Hit
ACACAGGGGTGGCCTTTCTAAGTGGTAAGAATGACCTGTCACTATAATATAC660.10160880609652836No Hit
CACCGGGGGACACTGTGTGGTCACTGTTTAGATTTGCCCATGGGTCTGTTTA660.10160880609652836No Hit
GTAGCCAGGGAACATTATATGGGTGGGAGGCTCTTTGCCTCTGTGATCAGTA660.10160880609652836No Hit
TTCATTGGGGTTCCTTCCTTCTTAAATCATATTTATATATGCATCTCTTTCA650.10006927873142946No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)