FastQCFastQC Report
Sat 31 Dec 2022
SRR5849772.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849772.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64125
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC16242.532553606237817No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT11871.8510721247563353No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA3430.5348927875243664No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC1900.2962962962962963No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT1800.2807017543859649No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA1500.23391812865497078No Hit
AACGAGGAGTGTTCCTTGCATTTTGGGACAGAATGGAATCTCAGACCTTGTG1420.22144249512670564No Hit
CTTTCCGGGGTTGGTGACTTGTTGACACGTGTTCCCATTTGATTTGGCTTCA1410.21988304093567254No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1360.21208576998050682No Hit
TCAGCGGGAAGAATTTCATCCTATGTAAATTTGTCCTAAACCTTTTTCTTTA1330.2074074074074074No Hit
GTTTCTGGGACTCGCGTCTGGCTACCAGCTCCCCGCTGCCCTGAGCTCGGCG1290.20116959064327486No Hit
GCATCTGGGTAACATCAAGCTTTAACAAATATTTAAAGACAAAAAAATCACG1240.19337231968810917No Hit
AACTGTGGGGATTCTTGCCCTTTCCAGCAGCTGCAGCTGCCGTTTCTCTCTG1230.19181286549707602No Hit
ACTACAGGGACACTAGTGGTGGTGGAGATGGAAAGGGACAGATTCAAGACAG1230.19181286549707602No Hit
CTTAATGGGCATTCTGAGGGAACACTAGATATAAATGGCGTAACTCCCAAAG1210.18869395711500975No Hit
ACATTTGGGACAGGAATTTTCTTGAAGATGGAGAAAGTGATGGCTTTTTAAG1180.18401559454191033No Hit
TTAAAAGGGAAGAGAGTGATTATTTGCATTACAAAGGAATTCTTAATAATTC1180.18401559454191033No Hit
GCACATGGGATATTCCAAGAGCTCACACCATTTGCAATAATAATAAAACAAT1180.18401559454191033No Hit
CTAGCAGGGGGATACTTGAGAAATAATTTTTGATTGTCTAATCAAAATGATA1160.18089668615984406No Hit
ACTGCAGGGGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGA1160.18089668615984406No Hit
ATAAGGGGACCCAAACTCAAAAAATATGAGTACTTGCGTACCTCCATTTCTG1140.17777777777777778No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC1120.17465886939571149No Hit
ATCTTCGGGAGCAAGCACAATTCTGTACTTTGGTTATACCATGATAATGGTT1120.17465886939571149No Hit
GCCACGGGGGGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTT1120.17465886939571149No Hit
CTATAGGGAGTGTTTTTATTACTTTCATCTGAAACATTCCATGTTTTAATCT1100.17153996101364521No Hit
CTCTATGGGAAGAAAAACAACTCCTCTTTCCCATAGTCAGAGTTGAATTTGT1070.1668615984405458No Hit
ACTGCAGGGAACTTTAAAAACTCCTGTATTTTGTATGGTTAATTTGCTGTTA1070.1668615984405458No Hit
ACATCGGGGATGTTCTTCAAAGTTTTGGTAGCTAAATCGGAAAGTTCTATAG1070.1668615984405458No Hit
TTCTATGGGATTGACCAATGGCCTCTGAAAAAGCACATTTTAGATACTGAAA1060.16530214424951267No Hit
GAAGGTGGGGGAGGAATACATTATTTTATCGTATATTTTTGTCCAAGCGATG1040.1621832358674464No Hit
GACAACGGGAAACTGATTCTAGGCAGAAAAAGACAACTTTGTTTTCTTTTTA1040.1621832358674464No Hit
TGCTCCGGGTAAGATACATATACACAAATATATAAGCTTTCCCCATGAAATA1010.15750487329434698No Hit
TGTTAACGGGATGCCACAGAACAAACACTACTCTAGCAGAGCCTTTCTTGCA1010.15750487329434698No Hit
TTTGGCTGGGAAGAGGATTCATGGCGAATATATGTGATAACATCTTTATACT1000.15594541910331383No Hit
GAACATTGGGACGCTCTTCCGATCTATAATAGGGTGCGAGAGAAGGATAAGC990.1543859649122807No Hit
AGGACTGGGATGTGATAGAAGTAGCATGTTGCTGCCAGAATACAAGCATTGC990.1543859649122807No Hit
CGCATCGGGGAAAGCCCTGGATCTCTTTGCACCAAAAATGGTCCAGATAGAC980.15282651072124756No Hit
ACGGCTAGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT960.1497076023391813No Hit
ATTGGGGGTTACAGACTAACAGCATCTCAAGGCAGAAGAATTTTTCTTAGTA960.1497076023391813No Hit
GCACTGGGATCTAGACATTATGCATATAATGTCCAAAACATATGCATATACA950.14814814814814814No Hit
TAAGCTAGGGACTGTAAGCAAATTGCTTAGCTTCTCTAGACATCAGTTTCCA910.1419103313840156No Hit
TTACAGGGATATGGAAGTTCAGAAGAACTGGGCTAGCCAAAACAACTTTGAA910.1419103313840156No Hit
GTTGTCGGGGCGCGCTGGTGCAGCCACCCTCGCTCCCTCTGCTCTTCCTCCC900.14035087719298245No Hit
TAGGTAGGGACGGGCGCCTGCCACCACACCCAGCTAATTTTTGTATTTTTTA900.14035087719298245No Hit
TAGCTTGGGACCATTAAGTTGTTTTTGAAAAGTCAACTCTGAGTTGTCCTGT890.1387914230019493No Hit
ATAAATGGGATTGCTTCACTACCCATCGGTGTCCATTTATAGAATAATGTGG890.1387914230019493No Hit
GCCATGGGATCAATGGTTATCTCAAAATCGTCTTTTAGGAGATCCCCAATTG890.1387914230019493No Hit
TGTTGTAGGGACTGAGAGAAGAACCACAGTTGAAACCTGCTTTTATCATTTT880.13723196881091618No Hit
GCTCACGGGGCTCTTCCACAGGAGGCCTACACGCCGCCGCTTGTGCTGCAGC880.13723196881091618No Hit
ACAAGTGGGACATTTTGTGCAGTGATTATTTTTTAAAGTCTTCTTTCATGTA870.13567251461988306No Hit
TGTAATGGGAAATCCACTCTGCAGGAAATAGGCCCAACCACCAGATGAGAAT870.13567251461988306No Hit
GGTTTGGGGAACTATACAAAATATTGTTGTATTTATACAAAACAACAATACC870.13567251461988306No Hit
TCGTTGGGGCTACAAAAGAAAGGAGAGTAGTATCATTACCCAGCTACAGTCA860.1341130604288499No Hit
TGATTAGGGGAGAGAGATTTCTGATATTGCCAGAAGACAAGTTGAAATTTTG850.13255360623781678No Hit
TGCCTTGGGGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCA840.1309941520467836No Hit
TATCGCGGGATCTCACAGAATTTTGATTTGCCTACTCACCCACAGGAGACAT840.1309941520467836No Hit
GATACAGGGACTCAGACTCTGAGGCCTGGTGTGAAGCTTACACTCTCTGCTC840.1309941520467836No Hit
GGCGTCTCGGGAAATGCATGTTTGTTAGAATGGAAACAGAAAAACATTAAAA830.12943469785575049No Hit
GCAATAGGGAGAGTGGATTAAGTTAATTTTATTTTGTCAGTGTTACTGTTTT830.12943469785575049No Hit
CACAGGGGGAGCTATGGGTCAGGTTATTCTAAGCTTTTATATACTCATGCCA830.12943469785575049No Hit
GCTAATGGGATTTAAATTTTATTTATATCAATCTTCTCTAGTGGGAGTAAGA830.12943469785575049No Hit
TGTAATGGGAGAATGGAAAGTTAAATATTTTTGCAGTGTGTATTTAAAAGAG830.12943469785575049No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC820.12787524366471734No Hit
AACAGAGGGTGCAGTTCTGGTACATAAAGATGAGTAATATAAACTGAAATCT810.12631578947368421No Hit
ACTGTTGGGAGAGGTGAGGGTCTAAAATCATTGAGATATGCAAAAATCACAC810.12631578947368421No Hit
CGTATAGGGGCTCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATG810.12631578947368421No Hit
GCAACAGGGCTGCAAGCCAGTCAAAGTGGCACAGCAAATCATATAAATCGAA800.12475633528265108No Hit
CTTGCTGGGGCATTTTCGTTGGTGGTGTTCAGTTGTGGCGGTTGCTGGTCAG800.12475633528265108No Hit
CGTAATGGGAAATTCTCGTAATATGCCATATGTTCACTTACCTCTCTGGGAT790.12319688109161794No Hit
CAAAGCGGGGCTACCTAAATTGAAATCCTTTTCAGAAAAAATATAATTGCAA790.12319688109161794No Hit
ACATGAGGGATAACAAAACATCTGGGCTTTATAAGTATGTAAAGAATAATGA790.12319688109161794No Hit
AACTAAGGGGAGGTTAGCTGAGGCTGGAAATGCCTTTTTCATGGTAAATGAT780.12163742690058478No Hit
ACACAGGGATGTCAGAAAAACACACACACACACACACACACACACACACACA780.12163742690058478No Hit
AAAGCGCGGGGTCCCAGGAAGAGCTTCCCCACTCCCATCCCCCAAATTGGAA780.12163742690058478No Hit
GTTATGGGATATTACCTAACTTCTAGTGTAGTACTGGTTCTAACAAGTAACA770.12007797270955164No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT770.12007797270955164No Hit
AATGCCGGGGAACTTTGTATGAGTATACCTGACTAAAAACACTAATGCGGTT770.12007797270955164No Hit
AGGCAGGGTCTAGGGACGGGGATAATGCACTGGAAGGCAAAACGGAGCCGTC760.11851851851851852No Hit
TAGCGCGGAACTCATATTTTAACAGTTTTGTGAAAAAAATAGAAAATATTTT760.11851851851851852No Hit
GCAATAGGGGAAGTGGCTTCAGGAGTATAAATTCAGCTAATTATTTCTATAT760.11851851851851852No Hit
CATTCAGGGAATATTTTAATGGTATAGATCTGCTAATGAATTCTCTTAAAAA740.11539961013645225No Hit
TGGCATGGGACACAGATAACTTTATGCTATTACATTCAGAGTTATTTCATTT740.11539961013645225No Hit
TATTATGGGATTGATATGAGTGAATATACTAAAAGAATTTATGTTCATCAAA740.11539961013645225No Hit
TGATAAGGGATAGGGAGGGCTCTGCATAGCACCTGCTATAGCGGTGTCATGT740.11539961013645225No Hit
GTACACGGGAAGCAGCTTACACTAGGCTTTTTTACAGCTGTGCTTGAGAACA740.11539961013645225No Hit
TCTACGTACGGGCAATAATTAATAGTTAAACAAACGATTACATTCATTTCAT740.11539961013645225No Hit
TGTAGCCGGGGTTTGGGAATGGTGCCCTTGGAGGCAACCTCCCATTTTAGAT730.11384015594541912No Hit
CAAAAGGGGAAAGTTAAAAAATTGTGCATGTCTGAGGGGTTGAATATATTTT730.11384015594541912No Hit
ATATAGGGGTCGCTCATTGCATACTCTTCAATCAGCCACATAGCCCTCGTAG730.11384015594541912No Hit
ACGCGTGGGAGGGTCTCCAATTGAGAAACATGGCACTGTTTTTCCTGCACTC730.11384015594541912No Hit
TTGTCCGGGATTCAACAATTGTCAAAAGCCCACCACAGAACATTTTCTAAAT720.11228070175438595No Hit
ACATCAGGGAATAAAATACAGGATCTTATATTTGTTTGATGATTTGGAAAAT720.11228070175438595No Hit
ACATAGGGGCAAACTCAATCGGGGACTAAAATTTCCCACTGAAAATGTTAAA720.11228070175438595No Hit
TACGCGGGGAAGGAATGATAGAGCACAATTCCACCTGAAAATCATGTTATAA720.11228070175438595No Hit
CGTCTAGGGGACCATATTCGCCAGTGTGGAGTTGAAGGTTAAGGAGTGAATG710.11072124756335282No Hit
TGATATGGGATTGAAACTGGGCTGGAAAACTGCAGTCCCAGGAACTCCAGAG710.11072124756335282No Hit
TGCTGGGACCTGCTGCTGATGCAATGCAATGCATCCCAATGGTTGTGGGGAT710.11072124756335282No Hit
GGCCATGGGATTCATTTAAGTATATAGTCCATAGTACTCTGTGGTCCCTGCA710.11072124756335282No Hit
GTTTGAGGGGTACCTTGCACACATCATCTGTAAGCTGTTTGGTTTTAAAATA700.10916179337231968No Hit
TGCTATGGGCAAGTTGACAACAGCAGAAACAGTAACAAAAAAATCTGCTCAG700.10916179337231968No Hit
TATCTTGGGCTGCCAACACTGGTTTCCCAGATGAGCAAACCGGCTCTTCTGA700.10916179337231968No Hit
CTCTACCAGGGATGTGAGTGAAGACCACCTGGATCTCCTGCCTCCTCCCTAG690.10760233918128655No Hit
TACCACGGGGGGCCACTGTTCAGGGTACTGACCATCAGTGTCAGCATTAGGG690.10760233918128655No Hit
TCTCGGGGGTCCCAGGCTCTCTGGGAAATACCCCGACCTTGGTCCAGTGGGC680.10604288499025341No Hit
GATTAGGGGATATATAAGAAAGACATCTTTCTCTTTTCTAAAAGACTTCCCT680.10604288499025341No Hit
GAGGCTGGGAGAGCGAGTCACATGGAGCATCCTAACTTTGCATTTTAGTTTT680.10604288499025341No Hit
CGCTTTGGGGATAATCAGGCTCTGTTTGCACTTTATATTTTAGCAGATACAG680.10604288499025341No Hit
CACGAGGGACCAGCAGATTCTGCGAAAGCACCAAATTTCTCAAGACCCTCTT670.10448343079922029No Hit
ACATTAGGGGAAGACCAGCTTTCTCATAAATTTCTCTTTTTGAAAAAAAGAA670.10448343079922029No Hit
TGCTTTGGGATTTTTTGTGGGGTATGGCAGTACAAGGAGGTGTTGGTCACGT670.10448343079922029No Hit
AATCTTGGGAGCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCC670.10448343079922029No Hit
TTCCGTGGGGAATTGTATTTATTATTTTTCTATTCCAGAAGAACTGGGTCCA660.10292397660818713No Hit
TGCAACGGGATGGACATCTGGTTGCTTTGGCCTGCTGCCCTCTTTCAGGGGT660.10292397660818713No Hit
GCAAATGGGAAGTTATACTAATACCATACTTCTTGATTACTGTAATTTAATA660.10292397660818713No Hit
AAGAGGGGGGGTCTGCAGTCTGGAAGCTTGTCTGCTCTATAGAAATGAAAAT660.10292397660818713No Hit
TTGACCGGGATAGCTAGTGAATGGCCCTGAAATTGTTTCCCAGTCATGGCTG650.10136452241715399No Hit
TGCAGAACGGGGCGCACCAAGGTTTCCCCACATCCTTCCCAGCACCCTTAGG650.10136452241715399No Hit
GCTGTTGGGGTGTAGATGGTGACTGTCTTGCTTGCAGCAGCTGAGGACACCG650.10136452241715399No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)