FastQCFastQC Report
Sat 31 Dec 2022
SRR5849771.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849771.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64440
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC16432.5496585971446306No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT11811.8327126008690255No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA3650.5664183736809435No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC1890.29329608938547486No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA1730.26846679081315955No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT1630.25294847920546243No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC1550.24053382991930478No Hit
ACTACAGGGACACTAGTGGTGGTGGAGATGGAAAGGGACAGATTCAAGACAG1540.23898199875853507No Hit
CTAGCAGGGGGATACTTGAGAAATAATTTTTGATTGTCTAATCAAAATGATA1480.22967101179391683No Hit
AACTGTGGGGATTCTTGCCCTTTCCAGCAGCTGCAGCTGCCGTTTCTCTCTG1470.22811918063314712No Hit
CTTTCCGGGGTTGGTGACTTGTTGACACGTGTTCCCATTTGATTTGGCTTCA1350.20949720670391062No Hit
AACGAGGAGTGTTCCTTGCATTTTGGGACAGAATGGAATCTCAGACCTTGTG1270.19708255741775296No Hit
AGGACTGGGATGTGATAGAAGTAGCATGTTGCTGCCAGAATACAAGCATTGC1250.19397889509621352No Hit
TTCTATGGGATTGACCAATGGCCTCTGAAAAAGCACATTTTAGATACTGAAA1240.19242706393544382No Hit
TGCTCCGGGTAAGATACATATACACAAATATATAAGCTTTCCCCATGAAATA1210.1877715704531347No Hit
GCATCTGGGTAACATCAAGCTTTAACAAATATTTAAAGACAAAAAAATCACG1200.186219739292365No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1190.18466790813159528No Hit
ACATCGGGGATGTTCTTCAAAGTTTTGGTAGCTAAATCGGAAAGTTCTATAG1160.18001241464928616No Hit
ACATTTGGGACAGGAATTTTCTTGAAGATGGAGAAAGTGATGGCTTTTTAAG1150.17846058348851646No Hit
ATCTTCGGGAGCAAGCACAATTCTGTACTTTGGTTATACCATGATAATGGTT1110.1722532588454376No Hit
GAAGGTGGGGGAGGAATACATTATTTTATCGTATATTTTTGTCCAAGCGATG1100.1707014276846679No Hit
ATAAGGGGACCCAAACTCAAAAAATATGAGTACTTGCGTACCTCCATTTCTG1090.1691495965238982No Hit
ACTGCAGGGAACTTTAAAAACTCCTGTATTTTGTATGGTTAATTTGCTGTTA1090.1691495965238982No Hit
GTTGTCGGGGCGCGCTGGTGCAGCCACCCTCGCTCCCTCTGCTCTTCCTCCC1090.1691495965238982No Hit
TCAGCGGGAAGAATTTCATCCTATGTAAATTTGTCCTAAACCTTTTTCTTTA1080.16759776536312848No Hit
GCAATAGGGGAAGTGGCTTCAGGAGTATAAATTCAGCTAATTATTTCTATAT1080.16759776536312848No Hit
TTAAAAGGGAAGAGAGTGATTATTTGCATTACAAAGGAATTCTTAATAATTC1070.16604593420235877No Hit
GCCACGGGGGGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTT1070.16604593420235877No Hit
GCACTGGGATCTAGACATTATGCATATAATGTCCAAAACATATGCATATACA1070.16604593420235877No Hit
ATTGGGGGTTACAGACTAACAGCATCTCAAGGCAGAAGAATTTTTCTTAGTA1070.16604593420235877No Hit
CACGAGGGACCAGCAGATTCTGCGAAAGCACCAAATTTCTCAAGACCCTCTT1050.16294227188081936No Hit
TACCACGGGGGGCCACTGTTCAGGGTACTGACCATCAGTGTCAGCATTAGGG1030.15983860955927995No Hit
TAGCTTGGGACCATTAAGTTGTTTTTGAAAAGTCAACTCTGAGTTGTCCTGT1020.15828677839851024No Hit
GGTTTGGGGAACTATACAAAATATTGTTGTATTTATACAAAACAACAATACC1020.15828677839851024No Hit
GTTTCTGGGACTCGCGTCTGGCTACCAGCTCCCCGCTGCCCTGAGCTCGGCG1010.15673494723774054No Hit
TGTTGTAGGGACTGAGAGAAGAACCACAGTTGAAACCTGCTTTTATCATTTT1000.15518311607697083No Hit
TTACAGGGATATGGAAGTTCAGAAGAACTGGGCTAGCCAAAACAACTTTGAA1000.15518311607697083No Hit
TGTAATGGGAAATCCACTCTGCAGGAAATAGGCCCAACCACCAGATGAGAAT1000.15518311607697083No Hit
GCCATGGGATCAATGGTTATCTCAAAATCGTCTTTTAGGAGATCCCCAATTG1000.15518311607697083No Hit
CGCATCGGGGAAAGCCCTGGATCTCTTTGCACCAAAAATGGTCCAGATAGAC980.1520794537554314No Hit
GAACATTGGGACGCTCTTCCGATCTATAATAGGGTGCGAGAGAAGGATAAGC980.1520794537554314No Hit
CAGTGTGGGATAGTTCACTTCAAATAATGTAAGATGCAAGATGATTAACCTA970.15052762259466168No Hit
ACTGCAGGGGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGA970.15052762259466168No Hit
TAGGTAGGGACGGGCGCCTGCCACCACACCCAGCTAATTTTTGTATTTTTTA960.14897579143389197No Hit
TGGCATGGGACACAGATAACTTTATGCTATTACATTCAGAGTTATTTCATTT950.14742396027312227No Hit
GCTCACGGGGCTCTTCCACAGGAGGCCTACACGCCGCCGCTTGTGCTGCAGC950.14742396027312227No Hit
GCACATGGGATATTCCAAGAGCTCACACCATTTGCAATAATAATAAAACAAT950.14742396027312227No Hit
CGTAATGGGAAATTCTCGTAATATGCCATATGTTCACTTACCTCTCTGGGAT930.14432029795158285No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC920.14276846679081315No Hit
GCTAATGGGATTTAAATTTTATTTATATCAATCTTCTCTAGTGGGAGTAAGA920.14276846679081315No Hit
CTCTATGGGAAGAAAAACAACTCCTCTTTCCCATAGTCAGAGTTGAATTTGT910.14121663563004344No Hit
TATCTTGGGCTGCCAACACTGGTTTCCCAGATGAGCAAACCGGCTCTTCTGA910.14121663563004344No Hit
CTTAATGGGCATTCTGAGGGAACACTAGATATAAATGGCGTAACTCCCAAAG900.13966480446927373No Hit
TGATATGGGATTGAAACTGGGCTGGAAAACTGCAGTCCCAGGAACTCCAGAG900.13966480446927373No Hit
GGCGTCTCGGGAAATGCATGTTTGTTAGAATGGAAACAGAAAAACATTAAAA890.13811297330850403No Hit
GATACAGGGACTCAGACTCTGAGGCCTGGTGTGAAGCTTACACTCTCTGCTC890.13811297330850403No Hit
TGTTAACGGGATGCCACAGAACAAACACTACTCTAGCAGAGCCTTTCTTGCA870.13500931098696461No Hit
ATATAGGGGTCGCTCATTGCATACTCTTCAATCAGCCACATAGCCCTCGTAG870.13500931098696461No Hit
TAAGCTAGGGACTGTAAGCAAATTGCTTAGCTTCTCTAGACATCAGTTTCCA840.1303538175046555No Hit
ATAAATGGGATTGCTTCACTACCCATCGGTGTCCATTTATAGAATAATGTGG840.1303538175046555No Hit
TCGTTGGGGCTACAAAAGAAAGGAGAGTAGTATCATTACCCAGCTACAGTCA840.1303538175046555No Hit
TTTGGCTGGGAAGAGGATTCATGGCGAATATATGTGATAACATCTTTATACT840.1303538175046555No Hit
TGCTATGGGCAAGTTGACAACAGCAGAAACAGTAACAAAAAAATCTGCTCAG830.1288019863438858No Hit
AACTAAGGGGAGGTTAGCTGAGGCTGGAAATGCCTTTTTCATGGTAAATGAT830.1288019863438858No Hit
GACAACGGGAAACTGATTCTAGGCAGAAAAAGACAACTTTGTTTTCTTTTTA830.1288019863438858No Hit
TAATCGGGAGGCAGGGGGAAATAAGCCAGCACCAAAAGACAAAAATTGCATG830.1288019863438858No Hit
CGCTTTGGGGATAATCAGGCTCTGTTTGCACTTTATATTTTAGCAGATACAG820.12725015518311608No Hit
TCTACGTACGGGCAATAATTAATAGTTAAACAAACGATTACATTCATTTCAT810.12569832402234637No Hit
ACGGCTAGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT790.12259466170080695No Hit
ATCTGCGGGATGTGTTTCTGTAACAAATTGGGATCTGTCTTGGCATTAAACC790.12259466170080695No Hit
CAAAGCGGGGCTACCTAAATTGAAATCCTTTTCAGAAAAAATATAATTGCAA790.12259466170080695No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT790.12259466170080695No Hit
TGCTGGGACCTGCTGCTGATGCAATGCAATGCATCCCAATGGTTGTGGGGAT790.12259466170080695No Hit
GTTATGGGATATTACCTAACTTCTAGTGTAGTACTGGTTCTAACAAGTAACA780.12104283054003724No Hit
TGTAGCCGGGGTTTGGGAATGGTGCCCTTGGAGGCAACCTCCCATTTTAGAT780.12104283054003724No Hit
TAGCGCGGAACTCATATTTTAACAGTTTTGTGAAAAAAATAGAAAATATTTT780.12104283054003724No Hit
ACTTGGGGGTTTAAACAATCTTTCATGTCTTGTCATACAAATTTATAAAGGT780.12104283054003724No Hit
ACATGAGGGATAACAAAACATCTGGGCTTTATAAGTATGTAAAGAATAATGA780.12104283054003724No Hit
ACGCGTGGGAGGGTCTCCAATTGAGAAACATGGCACTGTTTTTCCTGCACTC780.12104283054003724No Hit
AGTTATGGGAGTAAAAAGCTGAATGTTACTAATATTTTTCATATCTAAAAAA770.11949099937926753No Hit
CTCACACGGGGGTATGACTTTGTCCTGTTTATAGATCCTAGACAAGTGAGAA770.11949099937926753No Hit
CATTCAGGGAATATTTTAATGGTATAGATCTGCTAATGAATTCTCTTAAAAA760.11793916821849783No Hit
CTATAGGGAGTGTTTTTATTACTTTCATCTGAAACATTCCATGTTTTAATCT760.11793916821849783No Hit
AACAGAGGGTGCAGTTCTGGTACATAAAGATGAGTAATATAAACTGAAATCT760.11793916821849783No Hit
AATGCCGGGGAACTTTGTATGAGTATACCTGACTAAAAACACTAATGCGGTT760.11793916821849783No Hit
AGGCAGGGTCTAGGGACGGGGATAATGCACTGGAAGGCAAAACGGAGCCGTC750.11638733705772812No Hit
TGCTTTGGGATTTTTTGTGGGGTATGGCAGTACAAGGAGGTGTTGGTCACGT750.11638733705772812No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA740.11483550589695841No Hit
CTTGCTGGGGCATTTTCGTTGGTGGTGTTCAGTTGTGGCGGTTGCTGGTCAG730.11328367473618871No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT730.11328367473618871No Hit
AAAGCGCGGGGTCCCAGGAAGAGCTTCCCCACTCCCATCCCCCAAATTGGAA730.11328367473618871No Hit
TGATAAGGGATAGGGAGGGCTCTGCATAGCACCTGCTATAGCGGTGTCATGT730.11328367473618871No Hit
GATTTGGGGGAAAATCTCAAGTGGCTGAACTGAATGATGATGATAAAGATGA730.11328367473618871No Hit
ACAAGTGGGACATTTTGTGCAGTGATTATTTTTTAAAGTCTTCTTTCATGTA720.11173184357541899No Hit
CGTCTAGGGGACCATATTCGCCAGTGTGGAGTTGAAGGTTAAGGAGTGAATG720.11173184357541899No Hit
CAAAACCGGGGAAATGTAAAATATTTGAGAATAAGTTCATGTAATTGTCTTA720.11173184357541899No Hit
TGATTAGGGGAGAGAGATTTCTGATATTGCCAGAAGACAAGTTGAAATTTTG720.11173184357541899No Hit
AAGAGGGGGGGTCTGCAGTCTGGAAGCTTGTCTGCTCTATAGAAATGAAAAT720.11173184357541899No Hit
TTCCGTGGGGAATTGTATTTATTATTTTTCTATTCCAGAAGAACTGGGTCCA710.11018001241464928No Hit
TTCAAGGGGTCCGAGAAAAGTTGTGTAAGCGCCTGCGTTCTTCTGGGTTTGG710.11018001241464928No Hit
AACTGGGGACCTCACCGGCTGCCTCCGCGTCGGAAGCGACGGTGTCAGGACA710.11018001241464928No Hit
ACACAGGGATGTCAGAAAAACACACACACACACACACACACACACACACACA710.11018001241464928No Hit
ACTGTTGGGAGAGGTGAGGGTCTAAAATCATTGAGATATGCAAAAATCACAC710.11018001241464928No Hit
ACACAGGGGTGGCCTTTCTAAGTGGTAAGAATGACCTGTCACTATAATATAC700.10862818125387957No Hit
ACATTAGGGGAAGACCAGCTTTCTCATAAATTTCTCTTTTTGAAAAAAAGAA700.10862818125387957No Hit
ACATCAGGGAATAAAATACAGGATCTTATATTTGTTTGATGATTTGGAAAAT690.10707635009310987No Hit
TACGCGGGGAAGGAATGATAGAGCACAATTCCACCTGAAAATCATGTTATAA690.10707635009310987No Hit
TTCTAAGGGAAGACAACATGCATATATGACAATCATATATGATGTATTTGAT680.10552451893234016No Hit
GTTCACGGGACCTTATGGAAGCCACTCACGCATTCTCTGATTTACCCCCAAA680.10552451893234016No Hit
TCGTAAGGGTCTCTCAATATTATGAAAAATGTTAAAATTGTGTTTGCTGTTT680.10552451893234016No Hit
GTACACGGGAAGCAGCTTACACTAGGCTTTTTTACAGCTGTGCTTGAGAACA680.10552451893234016No Hit
TTGTCCGGGATTCAACAATTGTCAAAAGCCCACCACAGAACATTTTCTAAAT670.10397268777157045No Hit
GTTTGAGGGGTACCTTGCACACATCATCTGTAAGCTGTTTGGTTTTAAAATA670.10397268777157045No Hit
TGTAATGGGAGAATGGAAAGTTAAATATTTTTGCAGTGTGTATTTAAAAGAG670.10397268777157045No Hit
GGCCATGGGATTCATTTAAGTATATAGTCCATAGTACTCTGTGGTCCCTGCA670.10397268777157045No Hit
GTGACAGGGATCGTAAATACTAAACTGAATGGCTTTTATATTTTTAACTGCT660.10242085661080075No Hit
CTAGCTGGGGAGGTGCTTAAACTTTATAGAATACAGCTTAGCTCCCTATAAG660.10242085661080075No Hit
CGTATAGGGGCTCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATG660.10242085661080075No Hit
TGACTAGGGACTTTCGTCTATGACCTGCTTTCCTCTTTGAATCTAAGCTGGT660.10242085661080075No Hit
CACAGGGGGAGCTATGGGTCAGGTTATTCTAAGCTTTTATATACTCATGCCA660.10242085661080075No Hit
GTTATAGGGGCGATAATAATAAACATCTGCGTTTGTCTCTTCACTAAGAGTA660.10242085661080075No Hit
TTTTGCGGGATTCATTCCAGATTTTCGTGTACCCATTCTGTTTCAGGAGCTC650.10086902545003104No Hit
TGCTAAGGGATTGACTATGGGGACTTTTGGGGCCCCCTTAAATTTTGCACTT650.10086902545003104No Hit
TGCCTTGGGGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCA650.10086902545003104No Hit
TTGGTGTGGGCTTCCGATCTGAGAAGGGACCCCGCTAAATCCCCTAGAAGTC650.10086902545003104No Hit
CAAAAGGGGAAAGTTAAAAAATTGTGCATGTCTGAGGGGTTGAATATATTTT650.10086902545003104No Hit
TATTATGGGATTGATATGAGTGAATATACTAAAAGAATTTATGTTCATCAAA650.10086902545003104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)