FastQCFastQC Report
Sat 31 Dec 2022
SRR5849769.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849769.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73346
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC21162.8849562348321656No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT16482.246884629018624No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA3260.4444686826820822No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA2090.2849507812286968No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC2070.282223979494451No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT1950.26586316908897556No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1320.1799689144602296No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC1160.15815450058626238No Hit
TACTCAGGGAAAAGAAAAAGAAAAAGAAAATGTTGGTAGCACTCATTAATCA1130.15406429798489352No Hit
TCATAGGGACTGGTTAAGGTTTTTACATAACCGATATAAATGCGGAGACTTC1120.15270089711777055No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC1070.14588389278215583No Hit
ATTACAGGGGATCTGTGATTGGGACTCCAGATGTAGAGCAGACTGAGCTCAT1070.14588389278215583No Hit
TAAGTAGGGGAGGCTAACTTAGCTACTGTGAGTTTTTAAAAGACTATGAAAT1050.1431570910479099No Hit
TAAAAGGGGGGAAAAATGTGACAGACAAGCAGTTTTTCATTGCACACATATT1050.1431570910479099No Hit
GCTACAGGGTATGCATCAAGGTATTCTGGGGCATAAAAAAGCAAGTGTTCTC1040.14179369018078697No Hit
GTTTGCGGGGGATCAGAAATATTCCTTTTTCTCCTAGAGGAAATCCCTCATG1020.13906688844654105No Hit
TGATGAGGGAGGCATGAGCCACCGCGCTCTGCGAGGTACTTTTTCTATTTTT1010.13770348757941808No Hit
TTTACTGGGAAGAATAAGAGTTTGGTGCTGACTTCTCTTTGAATGTTTGAAC1010.13770348757941808No Hit
CATCACAGGGAAGAAGGATGCAGAGGAGGACGACTCGCTGGCCAATTCCTCA1000.13634008671229514No Hit
TGAAATGGGCAAGGTTAGAATTTACAGCTAACTGACTATATTCACAATCTTC1000.13634008671229514No Hit
AAAAGCGGGGAACATGGTACAAAGGTAGACAGTCCAAGGGACCCTTTCTCTT980.13361328497804922No Hit
CATGCAGGGACATTCATGTCATTGGCCCTTGTTCTGTCAACGTTGAATATTT970.1322498841109263No Hit
GCTTATGGGGGAAAACTGTATGGTGGCTTATCAAAAAAATTGAACATAGAAT970.1322498841109263No Hit
CGACTGGGGGATAGTGGAAAATAATGTAATACAATTTATAATGTTCAAAATA970.1322498841109263No Hit
GTAGTTGGGATAGAGAATAAATTAAAATCAGCAGATTGAGTTTTCAACATTG940.12815968150955745No Hit
ATACATGGGACTCTTTGAAGACATCTCTACACTTAATAATGCCTTATTGCTC940.12815968150955745No Hit
TTTGCTGGGAAGGCATGTACAGAAGTTCTAGTTTTTAAAGAAAAGCTAACAT940.12815968150955745No Hit
ATTTTGGGGACTACAGTATACTTGGTTGATTTAGGTTGCACATTCACTGAAT940.12815968150955745No Hit
CATCCAGGGTTGCATCACTTCTCATATTTTTCCCCCATCTCAGGGCCTATCT930.12679628064243448No Hit
GGATTGGGGGAATTAAGTATTGAATTCCAGGAGCCTGTTCCTGCTTCGATAC920.12543287977531153No Hit
GGTGATGGGAGAGCAACCGATTAAAAATCTAAGCAAATATTTGAACATTTTA920.12543287977531153No Hit
TTTACCGGGAGCAAAATCCCAAAGAAAAAATATCCCCCCAAATCAATACTCT910.12406947890818859No Hit
CAGTAAGGGATTATTGGAGAAATAAAATTATTTTTATGAATTTACTGTAGCC900.12270607804106563No Hit
GATTAAGGGGAGAAATCATTTTTGGATAATATGGATAAATTAGCTTTAAGAA890.12134267717394268No Hit
TCACTTGGGCTCCCTGCAAGAGACAGCTTGGCTTGGCTTTGGCTGTTGGGGA890.12134267717394268No Hit
TAATCTGGGATCGGACCCTTTGAGAACAATAAGCATTTCATCTTGTCTTGGA870.11861587543969677No Hit
CAATTGGGGGCTAGTGGTGTGGGACAAAATATTCCTAATGAAAGGAAGTACC870.11861587543969677No Hit
CTTACCGGGACTTCTTTGGTTGAATCTATGAACTTGATTGTATAATTTTCTT870.11861587543969677No Hit
TACCAAGGGGACCATGTGTTCTCATGTCTTTTTGCAGATTTAAAGGTTTTAT860.11725247457257382No Hit
ACTCTGGGGGGTATATGGTTAGTTTATTTGTTTCATTTAAAAAATCTCTTTC850.11588907370545087No Hit
AAGATTGGGATCCAGGTTTCTATTTGTATGCAGTCAGCTTACAATGAAGGAA840.11452567283832793No Hit
GTATAGGGGGGCTCTGGACAAGCACTGGGGGACGTGCCCCCAGAGCCACCCA840.11452567283832793No Hit
TAGCCTGGGTGACAGAGTGAGAAGACAAAAGAAAGAAAGAAAAGAAGGAAGG840.11452567283832793No Hit
CCATTCCGGGATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCC830.11316227197120499No Hit
TTTGTGGGGATATTTGATAATGAAAAAAATCTTTGCTTGTTTATTTCTGAAA830.11316227197120499No Hit
GATACGGGGATCCTACTTGTTTTGCAAGGAGCAGTAGAACTACTCATTTAAA830.11316227197120499No Hit
AATCGAGGGATTCTTCTTTTGGGTAAAACAAATTTACTTGGGCTCATTTAGA830.11316227197120499No Hit
GACACAGGGCTCATTAAAATCTTTCATGTAGGTGCCAGAACCATATGTAAAC830.11316227197120499No Hit
CGGCGAGGGGTATTTATCTTCTCATTATAAATATTTCTAAAATAAAAATAGA810.11043547023695907No Hit
GCAGTTGGGATACTCAAGGTTCCCAGGACTCTGCACTCTTGCAGCTCATTGA810.11043547023695907No Hit
AAGCATGGCACATTATGTAATTTAAAAAATATTCCTTTTTAAACAGCAACAG810.11043547023695907No Hit
CTACTAGGGAGAGGTATATATACCCAAGAGAATGCAAACATATGTCCACACA810.11043547023695907No Hit
TCATTAGGGAAGGAACTGTAAGCTTTTATCTTTTAACCAACTGAACAATACA800.10907206936983613No Hit
TTGTAAGGGGGGATGCATTTGTAATAAAAAGTTTCCTTAGAAGCATAGATCT800.10907206936983613No Hit
TCAAGGGGCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTA800.10907206936983613No Hit
TGCTCTGGGGGCATAATCCTTTTTTTGAGCTCTACAGAGAACAGTCTTTTGG800.10907206936983613No Hit
TGATGGGGATAGAAATGTAAAAATGTTTTAGTCTAGGGTTAAATAGTGTGAT790.10770866850271317No Hit
TACGTCGGGATTCTTATGCAATTTTCTCATAAAATATTAAAAATTGTTTATC780.10634526763559021No Hit
CATAGCGGGAGATAGAGATTTAACAGAGTTTTCAGAATTAGAATACTCAGAA780.10634526763559021No Hit
TTGGCTAGGAGAGTCTAGTGAAAAAAATGAGTGGGAAGAATGAATATAAAAG780.10634526763559021No Hit
CTGCGCTGGGACCTCAGATACAGTGACCGATGCCCTTGCTTTATATGTCTGT780.10634526763559021No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT770.10498186676846727No Hit
TTCTGAGGGGATCTGCTGTGGTGAGAGTAGAGAATGTTGAGTAAAAGTAGAA770.10498186676846727No Hit
GCTACTGGGACTTATTTAAATCCCCAAACACCATCTTTAAAACTTGAGCCTA770.10498186676846727No Hit
GATTCAGGGATGGCTTTTGTGCATTATCTAGATACTGTATCATGACAAAGAC770.10498186676846727No Hit
GTTAGGGCAGATATGAAGACTATGATTACCGATACAGAAGACGATCACCTTC770.10498186676846727No Hit
TAAAGGGAAGGTTCGCTGTATGTTTGCCTTGGACAAAAAGGCTACCTACCAC770.10498186676846727No Hit
AGATCAGGGATGTTATTACTGTTATTGACTAATTAACTTGGTTTAAATTCTA750.10225506503422135No Hit
CGAATAGGGTATGTCATTGCCGGGAACCTAGTATCTTCCAACTTGAATTGGT740.10089166416709841No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)