FastQCFastQC Report
Sat 31 Dec 2022
SRR5849768.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849768.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74342
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC19782.6606763337010033No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT16862.2678970164913506No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA3270.43985902988889186No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA2360.31745177692286997No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC2080.2797880067794786No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC2060.2770977374835221No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT1750.23539856339619597No Hit
TTTACCGGGAGCAAAATCCCAAAGAAAAAATATCCCCCCAAATCAATACTCT1330.17890290818110893No Hit
TACTCAGGGAAAAGAAAAAGAAAAAGAAAATGTTGGTAGCACTCATTAATCA1280.17217723494121762No Hit
CGACTGGGGGATAGTGGAAAATAATGTAATACAATTTATAATGTTCAAAATA1160.15603561916547848No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1130.15200021522154367No Hit
TGATGGGGATAGAAATGTAAAAATGTTTTAGTCTAGGGTTAAATAGTGTGAT1110.14930994592558716No Hit
ATTACAGGGGATCTGTGATTGGGACTCCAGATGTAGAGCAGACTGAGCTCAT1110.14930994592558716No Hit
TCATAGGGACTGGTTAAGGTTTTTACATAACCGATATAAATGCGGAGACTTC1090.14661967662963063No Hit
TAGCCTGGGTGACAGAGTGAGAAGACAAAAGAAAGAAAGAAAAGAAGGAAGG1040.1398940033897393No Hit
GTAGTTGGGATAGAGAATAAATTAAAATCAGCAGATTGAGTTTTCAACATTG1030.13854886874176106No Hit
GGTGATGGGAGAGCAACCGATTAAAAATCTAAGCAAATATTTGAACATTTTA980.13182319550186974No Hit
CATAGCGGGAGATAGAGATTTAACAGAGTTTTCAGAATTAGAATACTCAGAA970.13047806085389146No Hit
AAAAGCGGGGAACATGGTACAAAGGTAGACAGTCCAAGGGACCCTTTCTCTT970.13047806085389146No Hit
GCAGTTGGGATACTCAAGGTTCCCAGGACTCTGCACTCTTGCAGCTCATTGA960.1291329262059132No Hit
GCTACAGGGTATGCATCAAGGTATTCTGGGGCATAAAAAAGCAAGTGTTCTC960.1291329262059132No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC950.12778779155793496No Hit
TACCAAGGGGACCATGTGTTCTCATGTCTTTTTGCAGATTTAAAGGTTTTAT940.12644265690995668No Hit
CGGCGAGGGGTATTTATCTTCTCATTATAAATATTTCTAAAATAAAAATAGA940.12644265690995668No Hit
GGATTGGGGGAATTAAGTATTGAATTCCAGGAGCCTGTTCCTGCTTCGATAC940.12644265690995668No Hit
GTTTGCGGGGGATCAGAAATATTCCTTTTTCTCCTAGAGGAAATCCCTCATG940.12644265690995668No Hit
GCTTATGGGGGAAAACTGTATGGTGGCTTATCAAAAAAATTGAACATAGAAT940.12644265690995668No Hit
TTTGTGGGGATATTTGATAATGAAAAAAATCTTTGCTTGTTTATTTCTGAAA940.12644265690995668No Hit
GTCAATGGGATATGACCTTGAAAATCTTCCGGCATCCAAGGATTCCATTGTG940.12644265690995668No Hit
GATTAAGGGGAGAAATCATTTTTGGATAATATGGATAAATTAGCTTTAAGAA930.12509752226197843No Hit
GCTACTGGGACTTATTTAAATCCCCAAACACCATCTTTAAAACTTGAGCCTA930.12509752226197843No Hit
TTTACTGGGAAGAATAAGAGTTTGGTGCTGACTTCTCTTTGAATGTTTGAAC930.12509752226197843No Hit
TGAAATGGGCAAGGTTAGAATTTACAGCTAACTGACTATATTCACAATCTTC920.12375238761400016No Hit
ACTCTGGGGGGTATATGGTTAGTTTATTTGTTTCATTTAAAAAATCTCTTTC920.12375238761400016No Hit
CAATTGGGGGCTAGTGGTGTGGGACAAAATATTCCTAATGAAAGGAAGTACC910.1224072529660219No Hit
AAGTTCGGGAACTATCTTGTGGATAGTGGGCTGTGACAATCTGGAATAGAGA900.12106211831804364No Hit
TAATCTGGGATCGGACCCTTTGAGAACAATAAGCATTTCATCTTGTCTTGGA900.12106211831804364No Hit
TACGTCGGGATTCTTATGCAATTTTCTCATAAAATATTAAAAATTGTTTATC890.11971698367006538No Hit
ATTTTGGGGACTACAGTATACTTGGTTGATTTAGGTTGCACATTCACTGAAT890.11971698367006538No Hit
CATGCAGGGACATTCATGTCATTGGCCCTTGTTCTGTCAACGTTGAATATTT870.11702671437410885No Hit
CATCCAGGGTTGCATCACTTCTCATATTTTTCCCCCATCTCAGGGCCTATCT870.11702671437410885No Hit
GCTAGCGGGATCGTTATGATTATTATTACTACTACTACTTTTCTGACTTTTC870.11702671437410885No Hit
AGATCAGGGATGTTATTACTGTTATTGACTAATTAACTTGGTTTAAATTCTA850.11433644507815233No Hit
TTTGCTGGGAAGGCATGTACAGAAGTTCTAGTTTTTAAAGAAAAGCTAACAT850.11433644507815233No Hit
AGTCTGGGAGTTTTGTTGAAGATAGTTTATGTTTCTCTTCATATTTGATGAT850.11433644507815233No Hit
TAACAAGGGCTAAGGATTTTTACCTGAAAGCACAATGTATTGAGTCTCTTGA830.1116461757821958No Hit
GTTAGGGCAGATATGAAGACTATGATTACCGATACAGAAGACGATCACCTTC830.1116461757821958No Hit
TCAAGGGGCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTA820.11030104113421754No Hit
TAAAAGGGGGGAAAAATGTGACAGACAAGCAGTTTTTCATTGCACACATATT820.11030104113421754No Hit
CTTACCGGGACTTCTTTGGTTGAATCTATGAACTTGATTGTATAATTTTCTT820.11030104113421754No Hit
CTTGCCGGGGGACGGCTAGAAGCAATGTGTTTCGATGGTGTAAAGAGGTTAT810.10895590648623928No Hit
GAGACAGGGATAGTGAAAAATGTGAAGATGCTATCTGAATAGTGATAAGATA810.10895590648623928No Hit
ATTGTCGGGCCAGAGGCAGGGTGGATCTGCACCACTTCTCTGACCTTGCCAG800.10761077183826101No Hit
CTACTAGGGAGAGGTATATATACCCAAGAGAATGCAAACATATGTCCACACA800.10761077183826101No Hit
TGATGAGGGAGGCATGAGCCACCGCGCTCTGCGAGGTACTTTTTCTATTTTT800.10761077183826101No Hit
TAAAGGGAAGGTTCGCTGTATGTTTGCCTTGGACAAAAAGGCTACCTACCAC800.10761077183826101No Hit
TACGAAGGGAAGCGGTACCGCCAAAAGGGCAGGGAGGGCCACTCGCCACTCA790.10626563719028274No Hit
AAGATTGGGATCCAGGTTTCTATTTGTATGCAGTCAGCTTACAATGAAGGAA790.10626563719028274No Hit
TAAGTAGGGGAGGCTAACTTAGCTACTGTGAGTTTTTAAAAGACTATGAAAT780.10492050254230448No Hit
ATACTTGGGGGCCCAGATGTGGTGGGACCTGGTTTAACTACAAAGAAGTTAT780.10492050254230448No Hit
TTAGACGGGGAAAAAGGCTGGAAGTTGGACATAAGAAAGTGCCAGATGGAGC780.10492050254230448No Hit
TTAACCGGGGAGGTCTCATTAAACAGAGTGGAGATTTCATTGGTCAGCACTC780.10492050254230448No Hit
ATAGCTGGGACTTTTTTTCCTTTTTGAATTCTATCTTTATCTGTCTTTTGTT770.10357536789432623No Hit
TCACTTGGGCTCCCTGCAAGAGACAGCTTGGCTTGGCTTTGGCTGTTGGGGA770.10357536789432623No Hit
CCATTCCGGGATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCC760.10223023324634796No Hit
TTGTAAGGGGGGATGCATTTGTAATAAAAAGTTTCCTTAGAAGCATAGATCT760.10223023324634796No Hit
TCAGTTGGGACAGGAGGAGTTGCTACTGGAAGTGGGGCTCCTTTAAAGTCGC760.10223023324634796No Hit
TAGAACGGGATAAATATTCCATGAACAATTGGAGAAAATATATATTTTTTGT760.10223023324634796No Hit
ACAATGGGGAAGGAGGTCCTGAACAAAGCATGATAAATGTTAGTCCATGTGT750.1008850985983697No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)