FastQCFastQC Report
Sat 31 Dec 2022
SRR5849764.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849764.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16551
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC6724.060177632771434No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT5713.449942601655489No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA1290.7794090991480878No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA1080.6525285481239804No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT610.36855779107002595No Hit
GCTGCAGTGAGCCATAATCACATGATTGCACTCCAGCCTGGGTGACAAGGTT560.33834813606428615No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC560.33834813606428615No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT520.31418041205969427No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC500.3020965500573983No Hit
AGAGTTGGGATAAACAGAACTATAATAAATTGAAAGTATAATAATTGAAACT490.29605461905625036No Hit
AAACTTGGGATTGAAGCCAGCTTGTGTCGGATAAAGGTGTCCAAGGCAGCAG480.29001268805510244No Hit
TGTGTGGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA460.2779288260528065No Hit
TATGCGGAACAGAACACCATGACCTATATTAAATATTCTTAAATAAGCCTTT450.27188689505165853No Hit
TTTGTGGGGGAGGCTCGGGAGCCGGGAGGCTGCGGGCGGCGGCGCTGGACCC430.2598030330493626No Hit
TAATTAAGGGTAAATTAAAAATAGAACTACCATATGACCCAGCAATCCCTCT410.24771917104706664No Hit
GATTTAGGGACACAAAAAGCAGCAAATCATCAGTGACAATATCACTGGCTTC400.24167724004591867No Hit
ACGAACGGGTCTTCAGCAAGAGGTGACCCTGGGAGCGTACTTTAAAATAATT380.22959337804362273No Hit
GCACAAGGGGAAGATCCCCGATGTGTCCAGTTAACGCTCACTGGCCAGATGA380.22959337804362273No Hit
TGGCCAGGGTAATTACCAATACTCATCTCTATAAGAATCACAAAAACAGGAA380.22959337804362273No Hit
GAAACAGGGACAGACAGGAACTTGAAAAGACTATAGCCTTTTTTTAAGAACC370.2235514470424748No Hit
AATTGGAGAGGAAGCCGGGCGCCAAGACACACGGGGCTCCTGCCTTGGGAGC370.2235514470424748No Hit
GGTGTCGGGATATTGGTACATTTTGCCTGTTGGTATGCAAGGCAGCGGTGCT370.2235514470424748No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTC340.20542565403903087No Hit
TGGTCTGGAGATTGCCCCAGACATCCGGGGGAGAATTCCCTTATTGCTCACT340.20542565403903087No Hit
TAACCAGGGACCTTTAAAAAGTGAAGTCTGTGCAGGAAGCCAGTGACATAAA340.20542565403903087No Hit
AAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGAC340.20542565403903087No Hit
AGGCCTGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAG340.20542565403903087No Hit
CAAGTGGGGACAAGTGGAAAATTATACACCTGACCTCATGTGATGGGTTGCA340.20542565403903087No Hit
TAGTAGGGGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAAT330.19938372303788288No Hit
ACACTTGGGCATATCTATCTCCTATGGTTTCTTCATCAGCTTTTTTTTTACC330.19938372303788288No Hit
GACCAAGGGATTCCACGAGACCATCTTGGGATTCAGGTTCATTCTTTCTTTT320.19334179203673493No Hit
ATCACCGGGGTCATATGCTGCTCCCTGAGACAGTTATTTTGTGTGCAATTGT320.19334179203673493No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA320.19334179203673493No Hit
ATTAGTGGGAAGTATACAACTAAGTGAAATTGATCTGTGATTTTTCTTTTTT320.19334179203673493No Hit
ATTTGTGGGATCTATATTTGTAACAGAAGTAGTGTACAGATATTTTATTACA320.19334179203673493No Hit
AACCACGGGACCATTGAGATAATTACTGATAGGCAGTCTGGAAAGAAAAGAG320.19334179203673493No Hit
TTGTCTGGGCTGAAACATACGTCTGATCAATTCCAGTGTCTGGCACAATGTC320.19334179203673493No Hit
CATAGTGGGAACTATTAGTCAGGTGGATGAAGAAACGTTTTCTGTATTTGCA310.18729986103558696No Hit
TCAGCTGGGATATGCTAGAACTCTACCTGTGTTTCATTAGTATTATCTAATG310.18729986103558696No Hit
AGGAAAGGGGTTCATCTGTCTTTATGTATGTCATTTATCAGTTTATCTACAT310.18729986103558696No Hit
TATAATGGGGGCTAGTGACAGGGCTAATTAATTTGCTTTATACATTTTCTTT310.18729986103558696No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT310.18729986103558696No Hit
GCGAAGGGGATGTTCCCCAGAGTTAGTAGTGAATCAGTTCTGTTCTGTACAT310.18729986103558696No Hit
ACTAGTGGGAACGAGTAAATAATCTTACTTGGGTAGAGATGGCCTTTGCCAA300.18125793003443902No Hit
AGATAAGGGTACAGGACATCAAATATATCACATACATTCATATATCTCAAGA300.18125793003443902No Hit
AGTACCGGGATAAGAGAAAACTAACTTGCAATGCTGCAGAATGTCAGAGCAA300.18125793003443902No Hit
TACGTAGGGATGACTGCTGCTATTTAGAGTCAGAGAGGTGGAAGTCACTGGG290.17521599903329105No Hit
AGGTCTGGGAATATGATCTGGCAGAAACTCGCATGTATCCAAGTAAAGTAGT290.17521599903329105No Hit
GACCAAGGGAATGTCATCTTCTTGAGAAGGCTTTTTAAAATTGCCCTTTATA290.17521599903329105No Hit
GAGCGGGTGATGAGAATTTCCTTGAAAAAAGTTAATTTTAGATCATAGAGAC290.17521599903329105No Hit
CACACAGGGATCATGGAAGAACTGGGAATCTCACTGAAGAATCAGAAAAAAA290.17521599903329105No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT290.17521599903329105No Hit
GTATCAGGGTGGGATGTGAAAAATACTACCCTATTCTATCATTTGCTGTTGC280.16917406803214308No Hit
GTGTACGGGCTTTTCCTTTATGCGCTTCAATGGGAATAAAAATTGTTAAATC280.16917406803214308No Hit
GCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTAC280.16917406803214308No Hit
TCAAATGGGATGTTTTGGTGAATGTGAAAACTAAAGTTTGTGTCATGAGAAT280.16917406803214308No Hit
TTGCTAGGGAGTCTGGCTGGCTGAAATTTATTCTTACTTTTAAGTATTCTGA270.1631321370309951No Hit
TATGAAGGGCTGTGGTTGTGTGTAAAAAAAGAGTTAGGTTCTAGTCTCTGAT270.1631321370309951No Hit
CTTAGTGGGGAAATGGGGGTGATGGGGGAGAAAAAGAAGAACAAATTTGAAG260.15709020602984713No Hit
AATATAGGGCTACCTCATGGTGCACATTGGGGTTCTTGTCTTGTGCCACACA260.15709020602984713No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC260.15709020602984713No Hit
ATCGATGGGAAAAGATAACATTCTAGGACCTTTTCTAAGAACGTGTAATGTT260.15709020602984713No Hit
AACATGGGGCGATCTAAGGTAGGGTGGACTACCCTGAGAGCTAACCCCAGTT250.15104827502869916No Hit
GTGCAAGGGGCAAGGTTTCTTCACACCCTCTTTGATAAGAAGTGTTTAGCTG250.15104827502869916No Hit
TACCTAGGGTAGCTGGGACTACAGGCATGCGCCACCACGCCAGGCTAATTTT250.15104827502869916No Hit
ATTAAGGGGAGTGTGCCAAGGGCCGTGCAAGGGCAGGCTTGCTTTCCACCCA250.15104827502869916No Hit
ATATATGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT250.15104827502869916No Hit
CTGAGACAGGGGAATGTTAAAGGTGTATTGAAAATTTAGAGTTGGTTTTGTT240.14500634402755122No Hit
CTGCAAGGGTCAAAAATCAAATTGACATGTCATTAAAGAGAACACGTAACAA240.14500634402755122No Hit
ATTACAGGGGGGAGCGGGCGGCGGCGTTGGCGGCTTGTGCAGCAATGGCCAA240.14500634402755122No Hit
AAAAAAGGGAGGCTGAGGCAGAAGAATTGCTTGAGCCCGGGAGGCGGAGGTT240.14500634402755122No Hit
TAGTTGGGAATTTTAATGAAGTCCAGTCTATCAATTCTTTCTTTCGTAGATC240.14500634402755122No Hit
CAAAATGGGCAGTAACCCCCAGTTCCCTATCTCCTCTGCAACTGTAATTGCA240.14500634402755122No Hit
GGCAAAGGGCGATCTTAATGCGGGACCCCCTAATCAGATAGTAGATGATTGG230.13896441302640325No Hit
GTTACAGGGAATTTTGGCCAGTTTTCGTGCTTTATATATTTCATGCTTTCCA230.13896441302640325No Hit
TAGCAGTGGGACTTATGAACAGACAATTCCCTAAGTTGTTTTGGTGCTTAGA230.13896441302640325No Hit
TCAGCGGGACCACAAAGCCAAGACGTTCCATGGTATTTGTGCAAAAGAGATG230.13896441302640325No Hit
TGATATGGGCGATCTTGATACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCT230.13896441302640325No Hit
TGGTTGGGGAAGACCTTGCATATGTCTGTCCATTTTGATTTGAGATACAACT230.13896441302640325No Hit
TACACAGGGATCCTGACTCAAGTGACTAATCTCCTTTCTTACCCCTTTCCCC230.13896441302640325No Hit
GAATGCGGGAAGGCTACAGTTTTTAATGACTTAACAAATTGTACCACATTGT220.13292248202525528No Hit
TGTAATGGGGATCTTAACGAGGGACAAACACAACTATATGTGGTGCCATTTG220.13292248202525528No Hit
CTTATAGGGGCTCGGACCCGGGCAGTTAGTGGGGCTGCCCAGTGTGGACACG220.13292248202525528No Hit
GCTTTGGGACGGTATGGAGATGGTCCACGTCCTCCCAAGATGGCCCGATACG220.13292248202525528No Hit
CTATTTGGGTACATTAAAAATCCGATCAAGTAGGTATTACTACTATTCCTAT220.13292248202525528No Hit
CTATGGGGGGGGAATCATTATTTGTAAAAGAAGTGAGGTTGCCATTTAGTAA220.13292248202525528No Hit
TTATCAGGGTGTGTCAGTGTTTATAAAATGGACTTAAAGCACACACACATGC210.1268805510241073No Hit
TAAAGCGGGATTTTCAGTCTTTGGCAGCACTTCTGTCTCTTCCAGCATTTGT210.1268805510241073No Hit
GTAATTGGGAACCTAGTGAGTGACTGTACTCCTTTCTAGGAATGCTGATTCA210.1268805510241073No Hit
ATGTCGGGGATCTTTGGAATAGCTTTCTCGAAGTGCAATTGCTGAGTCAAAG210.1268805510241073No Hit
AACAATGGGAGGCCAGAGGTAATCAAAGAGTACTTTCGGAAGATGAAATATT210.1268805510241073No Hit
GCAGCCGGGGAACGTTTTATAGGAGCACAATTAATTTTACTTAGGATAAGTG210.1268805510241073No Hit
AAATGCGGGAGAATCATCTGTTCTTTAAGCCAAAATGGTTTTTATTTTTCCT210.1268805510241073No Hit
CTGATAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC200.12083862002295934No Hit
GCAGTTGGGGACAAAACAGAAAATGGAACCTTGTTTGAAGACCAAAATGTTG200.12083862002295934No Hit
ACGTATGGGATTGCTCAGGCTCAGGACAAGGCCTCAGGTCGTGGGCCCAGCT200.12083862002295934No Hit
GTCGGGAGAACTGATAATGTAGCAAGCCCCAAGTATGATAATAGGCAGACTA200.12083862002295934No Hit
GGACCAGGGTGCCATAGGTGGGAGTATAATTGTCACAGCCTTTAGGAATGTA200.12083862002295934No Hit
ATTCTTGGGAAGCTCCTCAGTGCTTCTTACCATTAGGCAAATTAGGGAAACA200.12083862002295934No Hit
TATCAAGGGGACTAGGTAAGTCTTTCTAAAAACCATTTAAAGCTAACTGGGT190.11479668902181137No Hit
TATGTAGGGCTCATAAGCCCTGCTCCTCACATGAGACCTTTCTAGCTTGATA190.11479668902181137No Hit
ATGTCTGGGGAGAACCAAGCAGTTGGTTAATGATAATGCAAACATTTAAAAG190.11479668902181137No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTC180.1087547580206634No Hit
TCGGCACGGGTGACATGAGATTTTTTAAGGGCTTTATGTGAACTATGATATT180.1087547580206634No Hit
GTAGAGGGGGAACACAGTGAAACAAATGGTATTTGAAGAAATGTAATATCTT180.1087547580206634No Hit
GTAAATGGGTATGATGCTGATAAAAGCAGCAGTAGAACAACAGATTTTAATT180.1087547580206634No Hit
GTTTGAGGGATAGGCATTGCTTTGAGTTAAAAATACCAAATTTCGGCTGGGC180.1087547580206634No Hit
GGTTAGGGGAAATGGACCTAATATATATCTACAGAACATTTCATCCAATGGC180.1087547580206634No Hit
CTTTTTTCGGGATTTGGTTAAATGTGTGTTAATTTGGCTGTAATGGCATTTA180.1087547580206634No Hit
TTCCTTGGGGAGAGCAGACTCACAGGTGCTGGTGCTTGGATTTAGCCAGGCT180.1087547580206634No Hit
TGTGCTCGGGCTTCCCCAACGAAAAGCACATCCAGGCAATGGCCTAAACTTC170.10271282701951544No Hit
CCACGACGGGTGGTAGATCAAGTAAACCAACATTATACTTTTTCTACAAGTG170.10271282701951544No Hit
CATGTCGGGATTTTTCTGATATATGCAGTATTACAGATATTCAGCAAAAGTA170.10271282701951544No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCG170.10271282701951544No Hit
TAGATGAGGGATGGCCTTTGGGAAGGTTTATCCAGGACAAAATGTTTATAAA170.10271282701951544No Hit
CTACATAGGGGAGCTCTGATTCTGGGATTCTAATCCACAGGACTTGGGTGTT170.10271282701951544No Hit
CACTGGGGGAGGCTGGGCACTCACAGTTTCTCTTTGGTCCCCACAGAACATG170.10271282701951544No Hit
ATTACTGGGATTACAGTTGGGGGATGAGCTTTGCTATTTAATTACTAATATT170.10271282701951544No Hit
AGACATGGATGAAATTGGAAATCATCATTCTCAGTAAACTATCGCAAGGACA170.10271282701951544No Hit
ACCGAGGGGATCTCGAACTGGGCGCTCTTCCGATCTGGCTGTGGGGATCTCA170.10271282701951544No Hit
TACGCGGGGAAGCCACAGAAGGTATACACCATAAAAGAAAGAATTGCTAAAT170.10271282701951544No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)