FastQCFastQC Report
Sat 31 Dec 2022
SRR5849763.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849763.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16462
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC6674.051755558255376No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT5493.334953225610497No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA1400.8504434455108736No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA1120.6803547564086988No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT640.38877414651925646No Hit
GCTGCAGTGAGCCATAATCACATGATTGCACTCCAGCCTGGGTGACAAGGTT570.3462519742437128No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC550.33410278216498607No Hit
TATGCGGAACAGAACACCATGACCTATATTAAATATTCTTAAATAAGCCTTT540.3280281861256226No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT520.3158789940468959No Hit
ACACTTGGGCATATCTATCTCCTATGGTTTCTTCATCAGCTTTTTTTTTACC520.3158789940468959No Hit
TTGCTAGGGAGTCTGGCTGGCTGAAATTTATTCTTACTTTTAAGTATTCTGA460.27943141781071557No Hit
AGAGTTGGGATAAACAGAACTATAATAAATTGAAAGTATAATAATTGAAACT460.27943141781071557No Hit
TCAAATGGGATGTTTTGGTGAATGTGAAAACTAAAGTTTGTGTCATGAGAAT450.27335682177135223No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC430.26120762969262545No Hit
AGGCCTGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAG430.26120762969262545No Hit
AAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGAC420.25513303365326206No Hit
GGTGTCGGGATATTGGTACATTTTGCCTGTTGGTATGCAAGGCAGCGGTGCT420.25513303365326206No Hit
GACCAAGGGATTCCACGAGACCATCTTGGGATTCAGGTTCATTCTTTCTTTT410.24905843761389868No Hit
TTTGTGGGGGAGGCTCGGGAGCCGGGAGGCTGCGGGCGGCGGCGCTGGACCC410.24905843761389868No Hit
TAATTAAGGGTAAATTAAAAATAGAACTACCATATGACCCAGCAATCCCTCT380.2308346494958085No Hit
TTGTCTGGGCTGAAACATACGTCTGATCAATTCCAGTGTCTGGCACAATGTC380.2308346494958085No Hit
TGGTCTGGAGATTGCCCCAGACATCCGGGGGAGAATTCCCTTATTGCTCACT360.21868545741708176No Hit
AGGAAAGGGGTTCATCTGTCTTTATGTATGTCATTTATCAGTTTATCTACAT360.21868545741708176No Hit
TAACCAGGGACCTTTAAAAAGTGAAGTCTGTGCAGGAAGCCAGTGACATAAA360.21868545741708176No Hit
AAACTTGGGATTGAAGCCAGCTTGTGTCGGATAAAGGTGTCCAAGGCAGCAG360.21868545741708176No Hit
TGGCCAGGGTAATTACCAATACTCATCTCTATAAGAATCACAAAAACAGGAA360.21868545741708176No Hit
GCTTTGGGACGGTATGGAGATGGTCCACGTCCTCCCAAGATGGCCCGATACG340.20653626533835498No Hit
ACGTATGGGATTGCTCAGGCTCAGGACAAGGCCTCAGGTCGTGGGCCCAGCT340.20653626533835498No Hit
GAAACAGGGACAGACAGGAACTTGAAAAGACTATAGCCTTTTTTTAAGAACC330.20046166929899162No Hit
TAGCAGTGGGACTTATGAACAGACAATTCCCTAAGTTGTTTTGGTGCTTAGA330.20046166929899162No Hit
ACGAACGGGTCTTCAGCAAGAGGTGACCCTGGGAGCGTACTTTAAAATAATT330.20046166929899162No Hit
AATTGGAGAGGAAGCCGGGCGCCAAGACACACGGGGCTCCTGCCTTGGGAGC330.20046166929899162No Hit
TAGTAGGGGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAAT330.20046166929899162No Hit
ATATATGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT330.20046166929899162No Hit
GATTTAGGGACACAAAAAGCAGCAAATCATCAGTGACAATATCACTGGCTTC320.19438707325962823No Hit
AGCTAAGGGTAATTCTGGAGAGAAGATTGTATTTTTTACAGTTTTTTGGGTT320.19438707325962823No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA320.19438707325962823No Hit
ATTCTTGGGAAGCTCCTCAGTGCTTCTTACCATTAGGCAAATTAGGGAAACA320.19438707325962823No Hit
CAAGTGGGGACAAGTGGAAAATTATACACCTGACCTCATGTGATGGGTTGCA320.19438707325962823No Hit
GCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTAC310.18831247722026484No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC310.18831247722026484No Hit
AACCACGGGACCATTGAGATAATTACTGATAGGCAGTCTGGAAAGAAAAGAG310.18831247722026484No Hit
CTGAGACAGGGGAATGTTAAAGGTGTATTGAAAATTTAGAGTTGGTTTTGTT300.18223788118090148No Hit
ATTACAGGGGGGAGCGGGCGGCGGCGTTGGCGGCTTGTGCAGCAATGGCCAA300.18223788118090148No Hit
AATATAGGGCTACCTCATGGTGCACATTGGGGTTCTTGTCTTGTGCCACACA300.18223788118090148No Hit
ACTAGTGGGAACGAGTAAATAATCTTACTTGGGTAGAGATGGCCTTTGCCAA290.1761632851415381No Hit
TCAGCTGGGATATGCTAGAACTCTACCTGTGTTTCATTAGTATTATCTAATG290.1761632851415381No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT290.1761632851415381No Hit
CATAGTGGGAACTATTAGTCAGGTGGATGAAGAAACGTTTTCTGTATTTGCA280.1700886891021747No Hit
ATTACGGGGAATTACTATCAATGGTTAAGAAATTTGGAAATTGTAGTCTTTT280.1700886891021747No Hit
GCACAAGGGGAAGATCCCCGATGTGTCCAGTTAACGCTCACTGGCCAGATGA280.1700886891021747No Hit
GCAGTTGGGGACAAAACAGAAAATGGAACCTTGTTTGAAGACCAAAATGTTG270.1640140930628113No Hit
GTGTACGGGCTTTTCCTTTATGCGCTTCAATGGGAATAAAAATTGTTAAATC270.1640140930628113No Hit
GAGCGGGTGATGAGAATTTCCTTGAAAAAAGTTAATTTTAGATCATAGAGAC270.1640140930628113No Hit
TACGTAGGGATGACTGCTGCTATTTAGAGTCAGAGAGGTGGAAGTCACTGGG260.15793949702344795No Hit
TATGTAGGGCTCATAAGCCCTGCTCCTCACATGAGACCTTTCTAGCTTGATA260.15793949702344795No Hit
TGATATGGGCGATCTTGATACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCT260.15793949702344795No Hit
ATTTGTGGGATCTATATTTGTAACAGAAGTAGTGTACAGATATTTTATTACA260.15793949702344795No Hit
CTATGGGGGGGGAATCATTATTTGTAAAAGAAGTGAGGTTGCCATTTAGTAA260.15793949702344795No Hit
GCAGCCGGGGAACGTTTTATAGGAGCACAATTAATTTTACTTAGGATAAGTG260.15793949702344795No Hit
AAATGCGGGAGAATCATCTGTTCTTTAAGCCAAAATGGTTTTTATTTTTCCT260.15793949702344795No Hit
CAAAATGGGCAGTAACCCCCAGTTCCCTATCTCCTCTGCAACTGTAATTGCA260.15793949702344795No Hit
ATGTCGGGGATCTTTGGAATAGCTTTCTCGAAGTGCAATTGCTGAGTCAAAG250.15186490098408456No Hit
CTTAGTGGGGAAATGGGGGTGATGGGGGAGAAAAAGAAGAACAAATTTGAAG250.15186490098408456No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTAT250.15186490098408456No Hit
CTATTTGGGTACATTAAAAATCCGATCAAGTAGGTATTACTACTATTCCTAT250.15186490098408456No Hit
TAGTTGGGAATTTTAATGAAGTCCAGTCTATCAATTCTTTCTTTCGTAGATC250.15186490098408456No Hit
ACCGAGGGGATCTCGAACTGGGCGCTCTTCCGATCTGGCTGTGGGGATCTCA250.15186490098408456No Hit
AACATGGGGCGATCTAAGGTAGGGTGGACTACCCTGAGAGCTAACCCCAGTT240.14579030494472117No Hit
AATCTTGGGGTATGTGCATATATAAGATGAGTGAATGAGCATTATCAATAGT240.14579030494472117No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT240.14579030494472117No Hit
ATTAGTGGGAAGTATACAACTAAGTGAAATTGATCTGTGATTTTTCTTTTTT240.14579030494472117No Hit
TATCGCGGGATTTTTGAGAGTGGGGTAAGCACCCCTACCTGCCTTACAGAGC240.14579030494472117No Hit
AGTACCGGGATAAGAGAAAACTAACTTGCAATGCTGCAGAATGTCAGAGCAA240.14579030494472117No Hit
GCGAAGGGGATGTTCCCCAGAGTTAGTAGTGAATCAGTTCTGTTCTGTACAT240.14579030494472117No Hit
CTGATAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC230.13971570890535778No Hit
AGGTCTGGGAATATGATCTGGCAGAAACTCGCATGTATCCAAGTAAAGTAGT230.13971570890535778No Hit
GTTTAAGGGGAAGAGATCAGTATCTATCATTGTAAAATATAACTTTATTAAA230.13971570890535778No Hit
AAAAAAGGGAGGCTGAGGCAGAAGAATTGCTTGAGCCCGGGAGGCGGAGGTT230.13971570890535778No Hit
AGATAAGGGTACAGGACATCAAATATATCACATACATTCATATATCTCAAGA230.13971570890535778No Hit
ATTAAGGGGAGTGTGCCAAGGGCCGTGCAAGGGCAGGCTTGCTTTCCACCCA230.13971570890535778No Hit
TGTGTGGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA220.1336411128659944No Hit
TACGCGGGGAAGCCACAGAAGGTATACACCATAAAAGAAAGAATTGCTAAAT220.1336411128659944No Hit
GCTCGGAGGGCTCGGCTCCAGAGCAGCTGTCAAACGCGTCGTTTCGCAGCAC220.1336411128659944No Hit
GAATGCGGGAAGGCTACAGTTTTTAATGACTTAACAAATTGTACCACATTGT210.12756651682663103No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATG210.12756651682663103No Hit
GACCAAGGGAATGTCATCTTCTTGAGAAGGCTTTTTAAAATTGCCCTTTATA210.12756651682663103No Hit
ATAAGGGGGTCAGTGTGGTGCTGCGTTCTGGCCAGTAAATTCCATGTTTTTG210.12756651682663103No Hit
GTAGATGGGCGATCTCAAGGTGGGCAAGTCCATAACCTGAGCCATATACCTC210.12756651682663103No Hit
AGCTGCGGAATTGTGAATAATTTGCAGATTTCAGAAGAATAGTGGCTTTGGG200.12149192078726764No Hit
AATGCAGGGGATCAAGGTTGCAGTGAGCCGTGATCGTGCCACTGCACTCCAG200.12149192078726764No Hit
ATCACCGGGGTCATATGCTGCTCCCTGAGACAGTTATTTTGTGTGCAATTGT200.12149192078726764No Hit
ATCGATGGGAAAAGATAACATTCTAGGACCTTTTCTAAGAACGTGTAATGTT200.12149192078726764No Hit
AGACATGGATGAAATTGGAAATCATCATTCTCAGTAAACTATCGCAAGGACA200.12149192078726764No Hit
ACACGTGGGGGCATATAGTTTGGACTGAATCACATCTGTAGTACTTAGCCAA190.11541732474790425No Hit
AACAATGGGAGGCCAGAGGTAATCAAAGAGTACTTTCGGAAGATGAAATATT190.11541732474790425No Hit
GTCGGGAGAACTGATAATGTAGCAAGCCCCAAGTATGATAATAGGCAGACTA190.11541732474790425No Hit
TGTTTAGGGGTCTACCCTAGAGATCCACTGCAGTATCTCACTCAGGCTTAAG190.11541732474790425No Hit
TACACAGGGATCCTGACTCAAGTGACTAATCTCCTTTCTTACCCCTTTCCCC190.11541732474790425No Hit
TTATCAGGGTGTGTCAGTGTTTATAAAATGGACTTAAAGCACACACACATGC180.10934272870854088No Hit
TGCTACGGGATCTTGAAAAAATGTGCTGGGGAGTATGGTATAATGAATTACG180.10934272870854088No Hit
AAAGCTGGTCTATGGCAGGGGGTACATTATCTGAGAGTAGTGTATTAGGTGG180.10934272870854088No Hit
TAAGCTGGGACCAAGAAGATCTCTCAAAACCATATAATTACATGGAAATTAA180.10934272870854088No Hit
GGCAAAGGGCGATCTTAATGCGGGACCCCCTAATCAGATAGTAGATGATTGG180.10934272870854088No Hit
GTATCAGGGTGGGATGTGAAAAATACTACCCTATTCTATCATTTGCTGTTGC180.10934272870854088No Hit
CTGCAAGGGTCAAAAATCAAATTGACATGTCATTAAAGAGAACACGTAACAA180.10934272870854088No Hit
TTACCTGGGATATACAAGGTGAGCCAAGGGCATCTTATAGTGCCAGAAAGTA180.10934272870854088No Hit
CTACATAGGGGAGCTCTGATTCTGGGATTCTAATCCACAGGACTTGGGTGTT180.10934272870854088No Hit
TACCTAGGGTAGCTGGGACTACAGGCATGCGCCACCACGCCAGGCTAATTTT180.10934272870854088No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTC170.10326813266917749No Hit
TCTCTAGGGATCTTCTCTCCAAATGAAATTATTTTAAAATTTAAATTTATTT170.10326813266917749No Hit
AACGCTGGGGATCTGTTATAGGGGTGTTTATTTTGCTTGAGTTTAAGTTGAG170.10326813266917749No Hit
TACTGCGGGCAGAAACATTGTTTTGAATCCTCTGTAAACCAAGGCATTAATC170.10326813266917749No Hit
AACATAGGGATATTACTGCATATAGAATAACAACAAGGTGTTCCGTGTGTGT170.10326813266917749No Hit
GCCCGGGAGATTTATTTCAGTTTATTGGTGATTACGTTACATTAACTGGACC170.10326813266917749No Hit
GTTACAGGGAATTTTGGCCAGTTTTCGTGCTTTATATATTTCATGCTTTCCA170.10326813266917749No Hit
TATCAAGGGGACTAGGTAAGTCTTTCTAAAAACCATTTAAAGCTAACTGGGT170.10326813266917749No Hit
TATATCAGGGGTTTAAAACAAAGTAAAAGCCAGTTTTTCTTTGTGGGGAATG170.10326813266917749No Hit
CTTATAGGGGCTCGGACCCGGGCAGTTAGTGGGGCTGCCCAGTGTGGACACG170.10326813266917749No Hit
TGCAAGGGGGAATAGTCACATGACCCAATCCACTGATTCTAGACAACAAAGT170.10326813266917749No Hit
TGACGGGTACTGCTCCTTTGTAAAAACACATTTTCCCAAAGAACACTGAATT170.10326813266917749No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGC170.10326813266917749No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)