FastQCFastQC Report
Sat 31 Dec 2022
SRR5849762.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849762.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16427
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC6844.163876544712973No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT5383.2750958787362268No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA1470.8948682047848054No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA1150.7000669629268886No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC600.36525232848359407No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT560.3409021732513545No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC560.3409021732513545No Hit
GGTGTCGGGATATTGGTACATTTTGCCTGTTGGTATGCAAGGCAGCGGTGCT480.29220186278687527No Hit
GCTGCAGTGAGCCATAATCACATGATTGCACTCCAGCCTGGGTGACAAGGTT460.28002678517075547No Hit
ACCGAGGGGATCTCGAACTGGGCGCTCTTCCGATCTGGCTGTGGGGATCTCA460.28002678517075547No Hit
TGGTCTGGAGATTGCCCCAGACATCCGGGGGAGAATTCCCTTATTGCTCACT430.26176416874657576No Hit
TAACCAGGGACCTTTAAAAAGTGAAGTCTGTGCAGGAAGCCAGTGACATAAA430.26176416874657576No Hit
TGGCCAGGGTAATTACCAATACTCATCTCTATAAGAATCACAAAAACAGGAA430.26176416874657576No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT420.25567662993851586No Hit
GTGCAAGGGGCAAGGTTTCTTCACACCCTCTTTGATAAGAAGTGTTTAGCTG410.24958909113045596No Hit
TATGCGGAACAGAACACCATGACCTATATTAAATATTCTTAAATAAGCCTTT410.24958909113045596No Hit
ACTAGTGGGAACGAGTAAATAATCTTACTTGGGTAGAGATGGCCTTTGCCAA400.24350155232239606No Hit
GATTTAGGGACACAAAAAGCAGCAAATCATCAGTGACAATATCACTGGCTTC400.24350155232239606No Hit
AAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGAC400.24350155232239606No Hit
AGAGTTGGGATAAACAGAACTATAATAAATTGAAAGTATAATAATTGAAACT380.23132647470627626No Hit
TACCTAGGGTAGCTGGGACTACAGGCATGCGCCACCACGCCAGGCTAATTTT380.23132647470627626No Hit
ATTTGTGGGATCTATATTTGTAACAGAAGTAGTGTACAGATATTTTATTACA370.22523893589821634No Hit
TTGCTAGGGAGTCTGGCTGGCTGAAATTTATTCTTACTTTTAAGTATTCTGA370.22523893589821634No Hit
ACGAACGGGTCTTCAGCAAGAGGTGACCCTGGGAGCGTACTTTAAAATAATT360.21915139709015644No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT360.21915139709015644No Hit
TACGTAGGGATGACTGCTGCTATTTAGAGTCAGAGAGGTGGAAGTCACTGGG340.20697631947403664No Hit
TCAGCTGGGATATGCTAGAACTCTACCTGTGTTTCATTAGTATTATCTAATG340.20697631947403664No Hit
TAATTAAGGGTAAATTAAAAATAGAACTACCATATGACCCAGCAATCCCTCT340.20697631947403664No Hit
AGGCCTGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAG340.20697631947403664No Hit
ATTACAGGGGGGAGCGGGCGGCGGCGTTGGCGGCTTGTGCAGCAATGGCCAA330.20088878066597676No Hit
GCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTAC330.20088878066597676No Hit
TGTGTGGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA330.20088878066597676No Hit
AGATAAGGGTACAGGACATCAAATATATCACATACATTCATATATCTCAAGA330.20088878066597676No Hit
GCACAAGGGGAAGATCCCCGATGTGTCCAGTTAACGCTCACTGGCCAGATGA330.20088878066597676No Hit
GCGAAGGGGATGTTCCCCAGAGTTAGTAGTGAATCAGTTCTGTTCTGTACAT330.20088878066597676No Hit
GAAACAGGGACAGACAGGAACTTGAAAAGACTATAGCCTTTTTTTAAGAACC320.19480124185791683No Hit
TTTGTGGGGGAGGCTCGGGAGCCGGGAGGCTGCGGGCGGCGGCGCTGGACCC320.19480124185791683No Hit
CTATTTGGGTACATTAAAAATCCGATCAAGTAGGTATTACTACTATTCCTAT320.19480124185791683No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT320.19480124185791683No Hit
ATAAGGGGGTCAGTGTGGTGCTGCGTTCTGGCCAGTAAATTCCATGTTTTTG320.19480124185791683No Hit
TCAAATGGGATGTTTTGGTGAATGTGAAAACTAAAGTTTGTGTCATGAGAAT320.19480124185791683No Hit
GTGTACGGGCTTTTCCTTTATGCGCTTCAATGGGAATAAAAATTGTTAAATC310.18871370304985696No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA310.18871370304985696No Hit
CTGATAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC300.18262616424179703No Hit
CACTGGGGGAGGCTGGGCACTCACAGTTTCTCTTTGGTCCCCACAGAACATG300.18262616424179703No Hit
AAATGCGGGAGAATCATCTGTTCTTTAAGCCAAAATGGTTTTTATTTTTCCT300.18262616424179703No Hit
CTGAGACAGGGGAATGTTAAAGGTGTATTGAAAATTTAGAGTTGGTTTTGTT290.17653862543373713No Hit
TAGCAGTGGGACTTATGAACAGACAATTCCCTAAGTTGTTTTGGTGCTTAGA290.17653862543373713No Hit
GCTTTGGGACGGTATGGAGATGGTCCACGTCCTCCCAAGATGGCCCGATACG290.17653862543373713No Hit
ACACTTGGGCATATCTATCTCCTATGGTTTCTTCATCAGCTTTTTTTTTACC290.17653862543373713No Hit
AGGTCTGGGAATATGATCTGGCAGAAACTCGCATGTATCCAAGTAAAGTAGT280.17045108662567726No Hit
TATCAAGGGGACTAGGTAAGTCTTTCTAAAAACCATTTAAAGCTAACTGGGT280.17045108662567726No Hit
ACGTATGGGATTGCTCAGGCTCAGGACAAGGCCTCAGGTCGTGGGCCCAGCT280.17045108662567726No Hit
AAACTTGGGATTGAAGCCAGCTTGTGTCGGATAAAGGTGTCCAAGGCAGCAG280.17045108662567726No Hit
TTGTCTGGGCTGAAACATACGTCTGATCAATTCCAGTGTCTGGCACAATGTC280.17045108662567726No Hit
GCTCGGAGGGCTCGGCTCCAGAGCAGCTGTCAAACGCGTCGTTTCGCAGCAC280.17045108662567726No Hit
GACCAAGGGAATGTCATCTTCTTGAGAAGGCTTTTTAAAATTGCCCTTTATA270.16436354781761733No Hit
TGATATGGGCGATCTTGATACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCT270.16436354781761733No Hit
CAAGTGGGGACAAGTGGAAAATTATACACCTGACCTCATGTGATGGGTTGCA270.16436354781761733No Hit
CATAGTGGGAACTATTAGTCAGGTGGATGAAGAAACGTTTTCTGTATTTGCA260.15827600900955743No Hit
CACTACGGGTGGTGAGCAGAGATTGCGCCACTGCACTGCGGCCTGGGTGACA260.15827600900955743No Hit
ATGTCGGGGATCTTTGGAATAGCTTTCTCGAAGTGCAATTGCTGAGTCAAAG260.15827600900955743No Hit
GACCAAGGGATTCCACGAGACCATCTTGGGATTCAGGTTCATTCTTTCTTTT260.15827600900955743No Hit
TCAGCGGGACCACAAAGCCAAGACGTTCCATGGTATTTGTGCAAAAGAGATG260.15827600900955743No Hit
TTATCAGGGTGTGTCAGTGTTTATAAAATGGACTTAAAGCACACACACATGC250.15218847020149753No Hit
TCGGCACGGGTGACATGAGATTTTTTAAGGGCTTTATGTGAACTATGATATT250.15218847020149753No Hit
AACATGGGGCGATCTAAGGTAGGGTGGACTACCCTGAGAGCTAACCCCAGTT250.15218847020149753No Hit
CTTAGGGTCTTCTGTCAAAGTTTTCTCCTCTTAAATGTTATTTTGCTTTTAC250.15218847020149753No Hit
AATATAGGGCTACCTCATGGTGCACATTGGGGTTCTTGTCTTGTGCCACACA250.15218847020149753No Hit
ATCGATGGGAAAAGATAACATTCTAGGACCTTTTCTAAGAACGTGTAATGTT250.15218847020149753No Hit
ATGTCTGGGGAGAACCAAGCAGTTGGTTAATGATAATGCAAACATTTAAAAG250.15218847020149753No Hit
AGTACCGGGATAAGAGAAAACTAACTTGCAATGCTGCAGAATGTCAGAGCAA250.15218847020149753No Hit
GCAGCCGGGGAACGTTTTATAGGAGCACAATTAATTTTACTTAGGATAAGTG250.15218847020149753No Hit
TATGAAGGGCTGTGGTTGTGTGTAAAAAAAGAGTTAGGTTCTAGTCTCTGAT250.15218847020149753No Hit
TGTAATGGGGATCTTAACGAGGGACAAACACAACTATATGTGGTGCCATTTG240.14610093139343763No Hit
AACGCTGGGGATCTGTTATAGGGGTGTTTATTTTGCTTGAGTTTAAGTTGAG240.14610093139343763No Hit
TATAATGGGGGCTAGTGACAGGGCTAATTAATTTGCTTTATACATTTTCTTT240.14610093139343763No Hit
AACAATGGGAGGCCAGAGGTAATCAAAGAGTACTTTCGGAAGATGAAATATT240.14610093139343763No Hit
ATTACGGGGAATTACTATCAATGGTTAAGAAATTTGGAAATTGTAGTCTTTT230.14001339258537773No Hit
AAAAAAGGGAGGCTGAGGCAGAAGAATTGCTTGAGCCCGGGAGGCGGAGGTT230.14001339258537773No Hit
AGGAAAGGGGTTCATCTGTCTTTATGTATGTCATTTATCAGTTTATCTACAT230.14001339258537773No Hit
TAGATGAGGGATGGCCTTTGGGAAGGTTTATCCAGGACAAAATGTTTATAAA230.14001339258537773No Hit
TACACAGGGATCCTGACTCAAGTGACTAATCTCCTTTCTTACCCCTTTCCCC230.14001339258537773No Hit
AGACATGGATGAAATTGGAAATCATCATTCTCAGTAAACTATCGCAAGGACA230.14001339258537773No Hit
TAGTTGGGAATTTTAATGAAGTCCAGTCTATCAATTCTTTCTTTCGTAGATC230.14001339258537773No Hit
ATTCTTGGGAAGCTCCTCAGTGCTTCTTACCATTAGGCAAATTAGGGAAACA230.14001339258537773No Hit
ATATATGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT230.14001339258537773No Hit
TATATCAGGGGTTTAAAACAAAGTAAAAGCCAGTTTTTCTTTGTGGGGAATG220.13392585377731783No Hit
GAGCGGGTGATGAGAATTTCCTTGAAAAAAGTTAATTTTAGATCATAGAGAC220.13392585377731783No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC220.13392585377731783No Hit
AATTGGAGAGGAAGCCGGGCGCCAAGACACACGGGGCTCCTGCCTTGGGAGC220.13392585377731783No Hit
ATTAGTGGGAAGTATACAACTAAGTGAAATTGATCTGTGATTTTTCTTTTTT220.13392585377731783No Hit
TAGTAGGGGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAAT220.13392585377731783No Hit
GACTCAGGGATGCCACTTTTTCCTGTTGAGTACACATGTATTTCTTTTCTGT210.12783831496925793No Hit
CTGTCGGGGATGACAGAGATCGTGATTATGACAGGGAACGTGGCAGACGTGA210.12783831496925793No Hit
GTATACGGGGTCCCTGGAATCCTGGAATACACCAGCCTGTGGCCAGAGGAAT200.12175077616119803No Hit
TGTGCTCGGGCTTCCCCAACGAAAAGCACATCCAGGCAATGGCCTAAACTTC200.12175077616119803No Hit
AATGCAGGGGATCAAGGTTGCAGTGAGCCGTGATCGTGCCACTGCACTCCAG200.12175077616119803No Hit
GGCAAAGGGCGATCTTAATGCGGGACCCCCTAATCAGATAGTAGATGATTGG200.12175077616119803No Hit
GTTTGGGGCTGTCAGCCTCCCACAGGTGGTACAGCCGTGCACACCAGTGTCG200.12175077616119803No Hit
CTTTTTTCGGGATTTGGTTAAATGTGTGTTAATTTGGCTGTAATGGCATTTA200.12175077616119803No Hit
GGACCAGGGTGCCATAGGTGGGAGTATAATTGTCACAGCCTTTAGGAATGTA200.12175077616119803No Hit
TACGCGGGGAAGCCACAGAAGGTATACACCATAAAAGAAAGAATTGCTAAAT200.12175077616119803No Hit
TACTGCGGGCAGAAACATTGTTTTGAATCCTCTGTAAACCAAGGCATTAATC190.11566323735313813No Hit
CTGCAAGGGTCAAAAATCAAATTGACATGTCATTAAAGAGAACACGTAACAA190.11566323735313813No Hit
GGATTTGGGGATGGCTTTTTCTTCCCTTTTCCTCCCGCGGGCCTGTTTTCAG190.11566323735313813No Hit
AATCTTGGGGTATGTGCATATATAAGATGAGTGAATGAGCATTATCAATAGT190.11566323735313813No Hit
ACACGTGGGGGCATATAGTTTGGACTGAATCACATCTGTAGTACTTAGCCAA190.11566323735313813No Hit
AACCACGGGACCATTGAGATAATTACTGATAGGCAGTCTGGAAAGAAAAGAG190.11566323735313813No Hit
CAAAATGGGCAGTAACCCCCAGTTCCCTATCTCCTCTGCAACTGTAATTGCA190.11566323735313813No Hit
GAATGCGGGAAGGCTACAGTTTTTAATGACTTAACAAATTGTACCACATTGT180.10957569854507822No Hit
AGCTGCGGAATTGTGAATAATTTGCAGATTTCAGAAGAATAGTGGCTTTGGG180.10957569854507822No Hit
GCCCGGGAGATTTATTTCAGTTTATTGGTGATTACGTTACATTAACTGGACC180.10957569854507822No Hit
GTATCAGGGTGGGATGTGAAAAATACTACCCTATTCTATCATTTGCTGTTGC180.10957569854507822No Hit
CTTAGTGGGGAAATGGGGGTGATGGGGGAGAAAAAGAAGAACAAATTTGAAG180.10957569854507822No Hit
GCAGCCGGGGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGAC180.10957569854507822No Hit
GCAGTTGGGGACAAAACAGAAAATGGAACCTTGTTTGAAGACCAAAATGTTG170.10348815973701832No Hit
TTTATGGGGGGGATGGAATCAGTTCTGCCTGGGAAACTAATCCGAATCATTT170.10348815973701832No Hit
TAAAGCGGGATTTTCAGTCTTTGGCAGCACTTCTGTCTCTTCCAGCATTTGT170.10348815973701832No Hit
CTTATAGGGGCTCGGACCCGGGCAGTTAGTGGGGCTGCCCAGTGTGGACACG170.10348815973701832No Hit
TAGAGAGGGGACTAAATGAATTTCAGAGTAAACACTGTGATTCTGCAGAGCG170.10348815973701832No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTAT170.10348815973701832No Hit
GTTTGAGGGATAGGCATTGCTTTGAGTTAAAAATACCAAATTTCGGCTGGGC170.10348815973701832No Hit
GTCGGGAGAACTGATAATGTAGCAAGCCCCAAGTATGATAATAGGCAGACTA170.10348815973701832No Hit
TGTTTAGGGGTCTACCCTAGAGATCCACTGCAGTATCTCACTCAGGCTTAAG170.10348815973701832No Hit
ATTAAGGGGAGTGTGCCAAGGGCCGTGCAAGGGCAGGCTTGCTTTCCACCCA170.10348815973701832No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)