FastQCFastQC Report
Sat 31 Dec 2022
SRR5849761.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849761.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93713
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC25512.7221410049833No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT19652.09682754793892No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA5360.5719590665115832No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA3920.418298421777128No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC1930.2059479474565962No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT1880.20061250840331651No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC1760.18780745467544524No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1510.16113025940904677No Hit
GACTTTGGGAAGGTAAGCTTTGAAAAGTTCTAAAACTCGCCATTATAAGACA1430.15259355692379928No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC1370.14619103005986364No Hit
AACGTATGGGCAACTGAGAAGTCATTTCCTTGGAGATCAACAGACGTGCCAA1340.1429897666278958No Hit
GTAAGTGGGATGTCTCAAAACAGTCTTTACTGTGAGTGCTATAGCACAGAGA1300.13872141538527205No Hit
TTCTACGGGGGGGGCGATGTGGAGCGCGGGCCGCGGCGGGGCTGCCTGGCCG1290.13765432757461613No Hit
TGGTTAGGGATTTCCAACTGAGCCAAAAACAATTGAGAAAATGATAGAAGCT1290.13765432757461613No Hit
CAAGGGCTACAGACAATTTAAAATCGAGCCTGGAAGCAAATATCCAGATAAT1260.1344530641426483No Hit
GATAGCGGGATAGTAAGCGGACGTTAAAAGCTTACCAAAAGTTATCAGTGTT1230.1312518007106805No Hit
GTAAATGGGGAAGGACATCTTGGTTGCTTCCAAGTTTCGTCAATAATGAAAA1200.12805053727871266No Hit
TGACATCGGGATCTATTGTCCTGCAATAAATATATTGCTAAAAAATTTGTTC1190.12698344946805673No Hit
TTATCTGGGGATAATCCTGTGTGAGATAATTTACATAGTGACTGGAAGAGTT1190.12698344946805673No Hit
AGCATAGGGCGATCTTACGGGGGGTAATAGAAAGATCTATGTTTATAAACCA1190.12698344946805673No Hit
CTTTCCGGGCGTCTTGCAGCATGCTAGGTATTATTTTAAACCAAAGTAGAGA1150.12271509822543297No Hit
GTGACGTGGGAAACGCATTTCTCCATTCTGCAGTCAATACCTTTGCTGGTAC1150.12271509822543297No Hit
AAGTTCGGGATGGTTTCACAGTTTAATATGAATGTAAAAGCCATTTAGGGTT1110.11844674698280923No Hit
ATAGCTGGGATTATTTCTTACAACTGCATGTGAATCTACAGTTATCTACAAC1110.11844674698280923No Hit
AATAAGGGGGTTTCTTGACTGTTACTTTGAATTGTTGTGAGCTTTTGCAAAT1100.11737965917215329No Hit
ATCACAGGGGTACTTCTGAGATTGGAACCCAGGGCAACAAAGCTAATGTGGG1100.11737965917215329No Hit
GAACTTGGGGTTCTTGGGAAGTTGACACAAGAACCACAACAGTTTAGAGAAA1090.11631257136149734No Hit
CAAAAAGGGGAGAGAGAAGGAAAGGAGAGAAGGCAGGAAGAAGGCAAGGGAC1080.1152454835508414No Hit
GTAACGGGGAGAGGAGATATATTTTGATGTTACTTTTCTCTTGTCACTTTTG1070.11417839574018546No Hit
TTAAACGGGGTTGTTATATCACTATGTTGTAAAGTTTTTGTAGACCAGGACT1070.11417839574018546No Hit
TTTTCCCGGGATGATATTCTCACTTTGTCGCAAATCTGCCCTTATCGTAAGA1070.11417839574018546No Hit
CATTGGGGGAATGTCTAGTTCTCAGAGCATTTGGAAAGGTTATCTTAAATGG1070.11417839574018546No Hit
GCTGACCAGGGAGTCCAGGGAAAATCTAAATTCCAGGGTGGAACTCTTGCTC1070.11417839574018546No Hit
TTAAAAGGGGGGGCCTCACTTCATTCTCTTCCACGTCATTTGGTGGTAGTGG1060.11311130792952952No Hit
GACCAGGGGAAACCAACATTATAAAATATCAATTAAAATGTATTTGTAAGTT1060.11311130792952952No Hit
AAACCAGGGATGTCATGAACAGGGCAGAATGCTTCAGGAAAGAATTGTCGAA1050.11204422011887358No Hit
AGTGCTGGGATTATAGACGTGAGCCACCACACCCGGCCCTTTTTCTGTATTT1050.11204422011887358No Hit
AAATTGGGAGTTCTTTGGCATTAAGGAAGTGGCAAACTTTCAGTACAAGGTC1050.11204422011887358No Hit
TCTTCTGGGACCTTTAAGATTATTTTCTATGATTTCATAAATAAAAGCATTG1050.11204422011887358No Hit
ATACTCGAGGTTTTGTTTTCCTTTTAAAATTCTTTAGGGAGAGAGGGATGGT1050.11204422011887358No Hit
CGTGGTGGGGCTAAAATTGACCACAAAATTGGAAGTAAAACACTCCTCAGCA1040.11097713230821765No Hit
CAAATGGGGACTCATGTTTCAACTTGCTGTGTATGATCATAAATCTATGATC1040.11097713230821765No Hit
AAGCCAGGGGGGATATATATACACATAGACACACATATATAATTCTCATATG1020.10884295668690577No Hit
TCTTGAGGGGAGGCCACCCATTCTTAGAATCAAATGTTACCTTACTGAGAAA1020.10884295668690577No Hit
GTCAATGGGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGT1020.10884295668690577No Hit
TGTAGCGGGATATTTTTGTGAGATTGTTGTATAATGTCTGCCTTCGTAGTAG1010.10777586887624982No Hit
ATATTAGGGCCTTGGAGGAAAAAGATACTTTGCATTAAGAAGTCTTCCCTTC1010.10777586887624982No Hit
AGACAGGGACTCAGGGCGCCGCCATGACGAGCCGGTCCGGAAGAAGAGGCAG1010.10777586887624982No Hit
GTATGAGGGAGTCTTTAAAATGCCATAAATTGTGGCAATTGTGAAGGGCAAG1010.10777586887624982No Hit
AGCTGCGGGACAGCAAAAGATGGAAAGGAAACAAGAAAATTAAATGAAGACA1010.10777586887624982No Hit
TGCCTTGGGATACCTACTCGTAGCCACAAGAATGTTGTAAAAATAGCAGGGA1000.10670878106559388No Hit
GAGCCAGGGCTGTGCACGGGATATAGTGCTGACTTTTTTTGTTTGAAATTAA990.10564169325493794No Hit
TTAGGTGGGGGTCAATTACTGAGAGCAAGAGTGAAAAATAACTGAATATAAT990.10564169325493794No Hit
AGGTTTGGGAAGAGCCTGGAATATTAAGTAAGATAATAGGTAAACATAATAT990.10564169325493794No Hit
AGCTCGGGGGGTCAATGATGAGAACCTTATATTGTCCTGAAGAGAGGTGATG990.10564169325493794No Hit
TGTCAAGGGAAGAACAAAAATAATATGTTCCAGCAGCACTGAGTCTATTTTT990.10564169325493794No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT980.104574605444282No Hit
TATAGAGGGTCGAGACTAGCCTGGACAACATGGGCAACCCTGTCTCTACCAA980.104574605444282No Hit
ATTCCTGGGGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA980.104574605444282No Hit
CTAATTGGGGGCATTCTCTCCATCGTGAAAATAAGTGAATAAGAGTGAAGCA970.10350751763362608No Hit
TTGTTGGGACATTTTCTCAGAGAAAAGTGGAATTTCTTGAAGATTTTGGTTC970.10350751763362608No Hit
TATATTGGGGACTTGATTTTATATCTTCTTACTGGAATTTATACTAGATTGT960.10244042982297014No Hit
AATAAAGGGAATATAACATCTAGTATTTCTCACACCTTCCGTCTGCTGAGCA960.10244042982297014No Hit
ACTATCGGGGGTCACCTTGAGCTTTGAGCTACCACTAAGCCATGAAAGAAAA950.1013733420123142No Hit
CGCAATGGGGGTTGAGTGTTCGGGACGCGGGCCTGCAGGCGCCATGGTCTTC950.1013733420123142No Hit
AACCATGGGATAGCATGATTTTTACGTAAACCTGTAGTCAGCAAATGCTGTA950.1013733420123142No Hit
GCACACTGGGACCGTGTTTGCATTGAACATCAACATGAATATTCTGATACTT950.1013733420123142No Hit
TTAAGCGGGGTCTCTTTGCTAGCCTAGGTGGCTGTATACAAATTCGGGTGGG950.1013733420123142No Hit
CATTCTGGGACTTGTCTTTTCTTTTTTCCTGATCAAGTATTTGATTCAAGTG940.10030625420165826No Hit
GCCAAGGGGAGGAAGAGGCCGAGGAAGAGGAGGCAGCTGAAGAGGAAGAAGC940.10030625420165826No Hit
TGCCTTGGGAAAGAAAACAGTGCCTGGTGCTTATAAATGGCCAAAAATAGTG940.10030625420165826No Hit
TGGTACGGGTACCTATACCTAATGTTGGATTTGTTCTTGTGCTCTTTTGAAG940.10030625420165826No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)