FastQCFastQC Report
Sat 31 Dec 2022
SRR5849760.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849760.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92272
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC24672.6736171319576902No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT19952.1620860065892145No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA5010.5429599445118779No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA3380.3663083058782729No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT2030.22000173400381481No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC1930.20916421016126235No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT1750.18965666724466795No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC1720.1864054100919022No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC1440.15606034333275534No Hit
TGGTTAGGGATTTCCAACTGAGCCAAAAACAATTGAGAAAATGATAGAAGCT1410.1528090861799896No Hit
AATAAGGGGGTTTCTTGACTGTTACTTTGAATTGTTGTGAGCTTTTGCAAAT1360.14739032425871337No Hit
TCTTCTGGGACCTTTAAGATTATTTTCTATGATTTCATAAATAAAAGCATTG1260.1365528004161609No Hit
CAAAAAGGGGAGAGAGAAGGAAAGGAGAGAAGGCAGGAAGAAGGCAAGGGAC1250.13546904803190565No Hit
AAGTTCGGGATGGTTTCACAGTTTAATATGAATGTAAAAGCCATTTAGGGTT1250.13546904803190565No Hit
TTCTACGGGGGGGGCGATGTGGAGCGCGGGCCGCGGCGGGGCTGCCTGGCCG1220.13221779087913993No Hit
GATAGCGGGATAGTAAGCGGACGTTAAAAGCTTACCAAAAGTTATCAGTGTT1170.12679902895786369No Hit
GTATGAGGGAGTCTTTAAAATGCCATAAATTGTGGCAATTGTGAAGGGCAAG1170.12679902895786369No Hit
TACTAAGGGTCTTCTGCAACAGGCGTGGGTCACGCTCTCGCTCGGTCTTTCT1170.12679902895786369No Hit
ATACTCGAGGTTTTGTTTTCCTTTTAAAATTCTTTAGGGAGAGAGGGATGGT1170.12679902895786369No Hit
GTAACGGGGAGAGGAGATATATTTTGATGTTACTTTTCTCTTGTCACTTTTG1160.12571527657360845No Hit
TATAATGGGAGTAAAAGCTTTCCAGGGCAGATCACTAGGATAATTTTTTTCT1160.12571527657360845No Hit
GCACACTGGGACCGTGTTTGCATTGAACATCAACATGAATATTCTGATACTT1150.12463152418935321No Hit
ATATTAGGGCCTTGGAGGAAAAAGATACTTTGCATTAAGAAGTCTTCCCTTC1150.12463152418935321No Hit
TTATAGGGGACCCTTTAATAGAAATTTTATCTTTTGTTATTTTGGTTAATTT1140.12354777180509798No Hit
CAAGGGCTACAGACAATTTAAAATCGAGCCTGGAAGCAAATATCCAGATAAT1140.12354777180509798No Hit
TTGTTGGGACATTTTCTCAGAGAAAAGTGGAATTTCTTGAAGATTTTGGTTC1130.12246401942084273No Hit
AACGTATGGGCAACTGAGAAGTCATTTCCTTGGAGATCAACAGACGTGCCAA1130.12246401942084273No Hit
AGGTTTGGGAAGAGCCTGGAATATTAAGTAAGATAATAGGTAAACATAATAT1090.11812900988382176No Hit
AGTGCTGGGATTATAGACGTGAGCCACCACACCCGGCCCTTTTTCTGTATTT1090.11812900988382176No Hit
AATAAAGGGAATATAACATCTAGTATTTCTCACACCTTCCGTCTGCTGAGCA1080.1170452574995665No Hit
GAATACGGGAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTT1080.1170452574995665No Hit
AACCATGGGATAGCATGATTTTTACGTAAACCTGTAGTCAGCAAATGCTGTA1080.1170452574995665No Hit
TGACATCGGGATCTATTGTCCTGCAATAAATATATTGCTAAAAAATTTGTTC1060.11487775273105602No Hit
TATATTGGGGACTTGATTTTATATCTTCTTACTGGAATTTATACTAGATTGT1060.11487775273105602No Hit
GTAAGTGGGATGTCTCAAAACAGTCTTTACTGTGAGTGCTATAGCACAGAGA1060.11487775273105602No Hit
AACTGGGGATTGTGATCAGGTAGCAATGACATGTACCACTTAAATTATTTTA1050.11379400034680076No Hit
TAAACTGGGGATGTTTTACCTAGAAGACAAATTGTACATTGGGCTTTAAGGA1050.11379400034680076No Hit
GTGTTAGGGGGGAGTAGGCGCTTCCGGCCATTCATACTGCAGTCGGTCAGTG1050.11379400034680076No Hit
GAGCCAGGGCTGTGCACGGGATATAGTGCTGACTTTTTTTGTTTGAAATTAA1040.1127102479625455No Hit
TATTACGGGAAGGACCAGGGAGCCCTGACTCCCAGAGGCTGCCACCGGGGAG1040.1127102479625455No Hit
GCTCCGGGGACTAGTGGGAGGCTTGAACATCCTACTGACAGCGTTAGATCAT1040.1127102479625455No Hit
GCTGACCAGGGAGTCCAGGGAAAATCTAAATTCCAGGGTGGAACTCTTGCTC1040.1127102479625455No Hit
AGCCTCGGGTAAAAAAGGATTACCTTTCCTTGTAAAGAGGATGCTGCCTTAA1040.1127102479625455No Hit
TTTTATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1030.11162649557829027No Hit
GCTTAAGGGATGTAGCAGTAAAAATAGTTAAAAATGTGGATAGATACTGTGA1020.11054274319403502No Hit
AGACAGGGACTCAGGGCGCCGCCATGACGAGCCGGTCCGGAAGAAGAGGCAG1020.11054274319403502No Hit
TTATCTGGGGATAATCCTGTGTGAGATAATTTACATAGTGACTGGAAGAGTT1020.11054274319403502No Hit
ATAGCTGGGATTATTTCTTACAACTGCATGTGAATCTACAGTTATCTACAAC1020.11054274319403502No Hit
TTAAACGGGGTTGTTATATCACTATGTTGTAAAGTTTTTGTAGACCAGGACT1020.11054274319403502No Hit
CAAATGGGGACTCATGTTTCAACTTGCTGTGTATGATCATAAATCTATGATC1020.11054274319403502No Hit
GTGACGTGGGAAACGCATTTCTCCATTCTGCAGTCAATACCTTTGCTGGTAC1020.11054274319403502No Hit
TGTCAAGGGAAGAACAAAAATAATATGTTCCAGCAGCACTGAGTCTATTTTT1010.10945899080977979No Hit
CGCAATGGGATGGTCAATTCACTTTTGACAAAGTCACGAAGGCAATTCACTG1000.10837523842552453No Hit
TGTAGCGGGATATTTTTGTGAGATTGTTGTATAATGTCTGCCTTCGTAGTAG1000.10837523842552453No Hit
TTAAAGGGAACTCACACTAGTACCTCACCTCAGCAGATACCAGAGCAACCAA1000.10837523842552453No Hit
TGGCGATGGGTTTGTGAACCAAGAGGGGTAAATGATGCTACCCATACAGTGA990.10729148604126929No Hit
ATTCCTGGGGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA990.10729148604126929No Hit
GCTTTGGGATTTATTTTTACTTTCATGCAATTTTTTTACACATCTTTTGGTG980.10620773365701405No Hit
AAACCAGGGATGTCATGAACAGGGCAGAATGCTTCAGGAAAGAATTGTCGAA980.10620773365701405No Hit
AAGCCAGGGGGGATATATATACACATAGACACACATATATAATTCTCATATG980.10620773365701405No Hit
GACATAGGGACTCTTACTAGTTTATTCTGCCTGCAACTATGAAATAAAACAG970.10512398127275879No Hit
GCAAAGGGAACAATATTCAGCACATAATAGGGATTTGATAAGTGTTCATTAC970.10512398127275879No Hit
GACTTTGGGAAGGTAAGCTTTGAAAAGTTCTAAAACTCGCCATTATAAGACA970.10512398127275879No Hit
CATTCTGGGACTTGTCTTTTCTTTTTTCCTGATCAAGTATTTGATTCAAGTG970.10512398127275879No Hit
TGCGCAGGGATTTGTGAGGATGATGCTGGTAAATGGAGCATGCTTAGAGTAC970.10512398127275879No Hit
CAATTGGGGGGCCTGAAGATATCTGGTAAGTAACAATTTTGGTTCTGCCCTC960.10404022888850356No Hit
CTTTCCGGGCGTCTTGCAGCATGCTAGGTATTATTTTAAACCAAAGTAGAGA960.10404022888850356No Hit
CGTGGTGGGGCTAAAATTGACCACAAAATTGGAAGTAAAACACTCCTCAGCA950.10295647650424831No Hit
AAATTGGGAGTTCTTTGGCATTAAGGAAGTGGCAAACTTTCAGTACAAGGTC950.10295647650424831No Hit
TATATGGGGGAAAATTATAGTGAAACACAGTAGAATAATTTAGAAGCATATA940.10187272411999305No Hit
AGCTCGGGGGGTCAATGATGAGAACCTTATATTGTCCTGAAGAGAGGTGATG940.10187272411999305No Hit
GAACTTGGGGTTCTTGGGAAGTTGACACAAGAACCACAACAGTTTAGAGAAA940.10187272411999305No Hit
GGCTATGGGATAAAAGAAATAGCCAAATAAAGAGATAATCTGTCTCTCCCAT940.10187272411999305No Hit
ATCACAGGGGTACTTCTGAGATTGGAACCCAGGGCAACAAAGCTAATGTGGG940.10187272411999305No Hit
TTTTCCCGGGATGATATTCTCACTTTGTCGCAAATCTGCCCTTATCGTAAGA940.10187272411999305No Hit
TTAGGTGGGGGTCAATTACTGAGAGCAAGAGTGAAAAATAACTGAATATAAT930.10078897173573782No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)