FastQCFastQC Report
Sat 31 Dec 2022
SRR5849759.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849759.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91449
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC22992.5139695349320386No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT19152.0940633577185097No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA4830.5281632385263918No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA3590.3925685354678564No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC2050.2241686623145141No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1880.2055790659274568No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT1600.1749609071723037No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC1600.1749609071723037No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC1380.15090378243611194No Hit
GTGACGTGGGAAACGCATTTCTCCATTCTGCAGTCAATACCTTTGCTGGTAC1360.14871677109645814No Hit
TGGTTAGGGATTTCCAACTGAGCCAAAAACAATTGAGAAAATGATAGAAGCT1320.14434274841715053No Hit
AAGTTCGGGATGGTTTCACAGTTTAATATGAATGTAAAAGCCATTTAGGGTT1250.13668820872836226No Hit
GCTGACCAGGGAGTCCAGGGAAAATCTAAATTCCAGGGTGGAACTCTTGCTC1250.13668820872836226No Hit
TGACATCGGGATCTATTGTCCTGCAATAAATATATTGCTAAAAAATTTGTTC1210.13231418604905465No Hit
TTATCTGGGGATAATCCTGTGTGAGATAATTTACATAGTGACTGGAAGAGTT1200.13122068037922777No Hit
TTATAGGGGACCCTTTAATAGAAATTTTATCTTTTGTTATTTTGGTTAATTT1180.12903366903957397No Hit
AATAAGGGGGTTTCTTGACTGTTACTTTGAATTGTTGTGAGCTTTTGCAAAT1150.1257531520300933No Hit
TTTGCTGGGGTTCCCTGCGTTCTGTGGTGGCTAATGAAATTTTTTTAATGTT1140.12465964636026639No Hit
TCTTCTGGGACCTTTAAGATTATTTTCTATGATTTCATAAATAAAAGCATTG1110.12137912935078568No Hit
TTAAACGGGGTTGTTATATCACTATGTTGTAAAGTTTTTGTAGACCAGGACT1100.12028562368095877No Hit
GAATACGGGAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTT1100.12028562368095877No Hit
AAACCAGGGATGTCATGAACAGGGCAGAATGCTTCAGGAAAGAATTGTCGAA1080.11809861234130499No Hit
AGTGCTGGGATTATAGACGTGAGCCACCACACCCGGCCCTTTTTCTGTATTT1080.11809861234130499No Hit
AGCTGCGGGACAGCAAAAGATGGAAAGGAAACAAGAAAATTAAATGAAGACA1080.11809861234130499No Hit
TAAACTGGGGATGTTTTACCTAGAAGACAAATTGTACATTGGGCTTTAAGGA1070.11700510667147809No Hit
GCAAATGGGATGGATAAATGGGGTTATTACCACCTTTATTAAAATTACCATA1070.11700510667147809No Hit
AGCATAGGGCGATCTTACGGGGGGTAATAGAAAGATCTATGTTTATAAACCA1060.11591160100165118No Hit
GTAACGGGGAGAGGAGATATATTTTGATGTTACTTTTCTCTTGTCACTTTTG1050.11481809533182429No Hit
GATAGCGGGATAGTAAGCGGACGTTAAAAGCTTACCAAAAGTTATCAGTGTT1050.11481809533182429No Hit
AATAAAGGGAATATAACATCTAGTATTTCTCACACCTTCCGTCTGCTGAGCA1050.11481809533182429No Hit
GTATGAGGGAGTCTTTAAAATGCCATAAATTGTGGCAATTGTGAAGGGCAAG1050.11481809533182429No Hit
GTTCAAGGGATATCACATGTTCTTACCTACAAGTGGAAGCTAAACATTAGGT1040.1137245896619974No Hit
CTAATTGGGGGCATTCTCTCCATCGTGAAAATAAGTGAATAAGAGTGAAGCA1040.1137245896619974No Hit
AACCATGGGATAGCATGATTTTTACGTAAACCTGTAGTCAGCAAATGCTGTA1040.1137245896619974No Hit
AAATTGGGAGTTCTTTGGCATTAAGGAAGTGGCAAACTTTCAGTACAAGGTC1040.1137245896619974No Hit
AACGTATGGGCAACTGAGAAGTCATTTCCTTGGAGATCAACAGACGTGCCAA1040.1137245896619974No Hit
ATACTCGAGGTTTTGTTTTCCTTTTAAAATTCTTTAGGGAGAGAGGGATGGT1040.1137245896619974No Hit
TATATGGGGGAAAATTATAGTGAAACACAGTAGAATAATTTAGAAGCATATA1030.1126310839921705No Hit
GTAAATGGGGAAGGACATCTTGGTTGCTTCCAAGTTTCGTCAATAATGAAAA1030.1126310839921705No Hit
CAAGGGCTACAGACAATTTAAAATCGAGCCTGGAAGCAAATATCCAGATAAT1010.1104440726525167No Hit
CAATTGGGGGGCCTGAAGATATCTGGTAAGTAACAATTTTGGTTCTGCCCTC1010.1104440726525167No Hit
TGCCTTGGGATACCTACTCGTAGCCACAAGAATGTTGTAAAAATAGCAGGGA1010.1104440726525167No Hit
AGCCTCGGGTAAAAAAGGATTACCTTTCCTTGTAAAGAGGATGCTGCCTTAA1010.1104440726525167No Hit
TTCTACGGGGGGGGCGATGTGGAGCGCGGGCCGCGGCGGGGCTGCCTGGCCG1000.1093505669826898No Hit
GCACACTGGGACCGTGTTTGCATTGAACATCAACATGAATATTCTGATACTT1000.1093505669826898No Hit
TGCTAAGGGGGTAGATATTTACGGTCACTGGGCGTTCCTCACATTCCACATT1000.1093505669826898No Hit
TTTTCCCGGGATGATATTCTCACTTTGTCGCAAATCTGCCCTTATCGTAAGA990.10825706131286292No Hit
TTAGGTGGGGGTCAATTACTGAGAGCAAGAGTGAAAAATAACTGAATATAAT980.10716355564303602No Hit
ATATTAGGGCCTTGGAGGAAAAAGATACTTTGCATTAAGAAGTCTTCCCTTC980.10716355564303602No Hit
AATTAGGGGAAGAATGACAAAGTTATATGATTTAGTGGAAGAGATTGCAAGG970.10607004997320911No Hit
CGCAATGGGGGTTGAGTGTTCGGGACGCGGGCCTGCAGGCGCCATGGTCTTC970.10607004997320911No Hit
TGGCGATGGGTTTGTGAACCAAGAGGGGTAAATGATGCTACCCATACAGTGA970.10607004997320911No Hit
CAAATGGGGACTCATGTTTCAACTTGCTGTGTATGATCATAAATCTATGATC970.10607004997320911No Hit
CAAAAAGGGGAGAGAGAAGGAAAGGAGAGAAGGCAGGAAGAAGGCAAGGGAC960.10497654430338221No Hit
ATAGCTGGGATTATTTCTTACAACTGCATGTGAATCTACAGTTATCTACAAC960.10497654430338221No Hit
TCTAACGGGTAGCATATATTTGAATGAATATAGATTGAAGAAATGTAATGTC960.10497654430338221No Hit
AACTGGGGATTGTGATCAGGTAGCAATGACATGTACCACTTAAATTATTTTA950.1038830386335553No Hit
CTGTCAGGGTCTGCAGAAGGGGTATTAGCATCCTTAGAAGGGACAGAACTAA950.1038830386335553No Hit
CGTGGTGGGGCTAAAATTGACCACAAAATTGGAAGTAAAACACTCCTCAGCA950.1038830386335553No Hit
GCTCCGGGGACTAGTGGGAGGCTTGAACATCCTACTGACAGCGTTAGATCAT950.1038830386335553No Hit
ATTCCTGGGGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA950.1038830386335553No Hit
GCTTAAGGGATGTAGCAGTAAAAATAGTTAAAAATGTGGATAGATACTGTGA940.1027895329637284No Hit
AAACCGGGAAGGAAAAATAGAGACCTTCCCAGAAAAACAAAAGCTGAGGAAT940.1027895329637284No Hit
AGCTCGGGGGGTCAATGATGAGAACCTTATATTGTCCTGAAGAGAGGTGATG940.1027895329637284No Hit
CTGTGCGGGAACCCAGGAATTGTGGGTGTTAAAGAGTCTGAAAGATCATCTA940.1027895329637284No Hit
CTTTCCGGGCGTCTTGCAGCATGCTAGGTATTATTTTAAACCAAAGTAGAGA940.1027895329637284No Hit
GCCAAGGGGAGGAAGAGGCCGAGGAAGAGGAGGCAGCTGAAGAGGAAGAAGC930.10169602729390152No Hit
GACCAGGGGAAACCAACATTATAAAATATCAATTAAAATGTATTTGTAAGTT930.10169602729390152No Hit
GCTAAAGGGCGGCAATAAATTTATCTTTTTATTTGTTTGTCTGATTTACTTG920.10060252162407463No Hit
GCACCGGGGATCCAAAGGCGGGGAGTTCAAGCTCCAGAAACTCAAACGCAGC920.10060252162407463No Hit
GCAAAGGGAACAATATTCAGCACATAATAGGGATTTGATAAGTGTTCATTAC920.10060252162407463No Hit
GATGGGGCTTCCTGAGTCAGTGCCTGAATCTTTATTTTTTAAATTGAATGTT920.10060252162407463No Hit
AGTTGGGGGATGCAAAGCAAGGGTGTTTTTTTTGTTTGTTTGTTTGTTTGTT920.10060252162407463No Hit
CATTCTGGGACTTGTCTTTTCTTTTTTCCTGATCAAGTATTTGATTCAAGTG920.10060252162407463No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)