FastQCFastQC Report
Sat 31 Dec 2022
SRR5849751.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849751.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84987
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC23402.7533622789367787No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT21142.4874392554155342No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA4540.5341993481355972No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA3450.4059444385611917No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC2870.3376987068610493No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT2440.28710273335921965No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC1560.1835574852624519No Hit
AACATTGGGACATAGGAATTTGAATGTGGGTCAGAATATCACTAATTTTGAT1560.1835574852624519No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1500.17649758198312684No Hit
TCGTCTGGGGCTGCCTTGTGTGTGCTGTACGTATCCTGCGTTAGGACCCTTG1460.1717909797969101No Hit
TGCGTTGGGCACTCTGCTCCACCAGCTTCTTGTGGAAGGCTGGGTATTATCC1400.16473107651758503No Hit
TTGGGTGGGAATCAATGTTTACTTTACATGTTAAAAATAAGAAACTAAGACC1340.15767117323825997No Hit
TCTAGAGGGAGGGCTTGAACTCCTGGGCTCAAGCAATCTGTCTGCCTCGGCC1330.1564945226917058No Hit
GTTACTGGGGACAGCAAACTGCTGAGGTCACTTCCCCAGGCCCACTGAATGA1320.1553178721451516No Hit
GGCACTGGGGTCCTTAGGGACACACCACAAGCATGGAGGAATCAGAGTAGAG1270.14943461941238073No Hit
GATCAAGGGGGCTCTGCTGCTGCGGAGCCCCCGCCTGCCCGCCCGGGAGCTG1260.14825796886582654No Hit
CCTGTTGGGACCTCAAGTGCAGGTGCAGGATGAAGAAATTACAGAGGATGAC1250.14708131831927237No Hit
TGATATGGGAACTACCATAGCTAATATTCCTTTCCTTTCTGCAGGTCAATTT1240.14590466777271818No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC1230.14472801722616402No Hit
AGTTCAGGGAACCTCAAAAGTTTCAAAAGTTCTTTAACCAGATAAACTCCAT1220.14355136667960983No Hit
AAGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTC1180.1388447644933931No Hit
TAGCCTGGGCTTTGTACAACAGTACAATTATTATCTGAATTTTACAGATTTT1160.13649146340028476No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT1140.1341381623071764No Hit
AAGTGCGGGCAACAAGTGATTATTGATGATCTACAGTGTAACAGGCACGACG1090.1282549095744055No Hit
CACCAGGGGCACAGGATGTGAGGAAAGAAAAGGAAATCAATTCTGACTGTGT1080.1270782590278513No Hit
CTAACTGGGGAGGGAAGGATGCGTTCCAGGTATCTTTCCTGAGTTTATGAAA1050.1235483073881888No Hit
TTTACAGGGAATTACCAGATCTTCCCAATATTATAACATCCGTGCATTCCAG1050.1235483073881888No Hit
TAACGTTGGGGGTGAACATGACGGCAGCCGTGCGGACCTACCGCTGGCAGTG1040.1223716568416346No Hit
GCCTCAGCCTCCCGAGTGGCTGGGACTGCAGGCGCCCGCCACCAAGTCCGGT1040.1223716568416346No Hit
AAATCAGGGGGAAGGTTTATGCTCTGAGCTCAGGATAGTTGGGGAACACATT1030.12119500629508044No Hit
CTTCAGGGGGAGAGTGAGGACTTTCCGCGCCCATTGGTGACCCTTCCAGGCA1030.12119500629508044No Hit
ATGCATGGGGTGTCTCTGGAGCTGGTTTCTGGGCCAGTGCTTTGTTCCTGGC1020.12001835574852625No Hit
GATGGGGGAGACGCCAGAGGTGCAGCTCCAGCAGCAATGGCAGTGACGGCGT1010.11884170520197207No Hit
TTCCCGGGAAGGGAAGGAAAAACAGGATAGATTAGTCTCTTTTTGAGCCCAA1010.11884170520197207No Hit
TAATTAGGGGTCCATTTAATTTCTTAAATGGAATCCTGAAATTTTGTGTCTA1010.11884170520197207No Hit
TAGTACGGGGAAGAATCTTAAAGGTATGCAGAGCAGAAGTGACAGTCTTGGG1000.11766505465541789No Hit
GATTATGGGACATTTTGATAGCCATTGATGTATGCATCTCTTGGCTGTACTA1000.11766505465541789No Hit
GTTGACGGGACAGAACATGGTTGTTATTGTGCTGTTGACATGAATAGACCAT1000.11766505465541789No Hit
TCCTAGGGATTTAAGTATTTGTTTATCTTTCTTTTAAAATATTTCTTTGAAT1000.11766505465541789No Hit
AAGACTGGGATATTCTAGATATGAACACTTGGCATTTGACATTTTCTAAAGC990.1164884041088637No Hit
AGCTTTGGGATATTTATGTAGAATCAAATTATATAAACAGTACCAATATCAT990.1164884041088637No Hit
TGCAACGGGGACTGATAGAGTTCTGTCTCCTTTCTCTTTTGCTACCCAACCT990.1164884041088637No Hit
ATCTGGGGGAAGAGATGCCAGACTTTGAAATAAGTACATTAATTAGGGCCAT980.11531175356230954No Hit
GCAGTGGGTATAAAGCACCAGACAGAAGAGTCTGGGTCATGTTATGCTACCA980.11531175356230954No Hit
TATAGGGGGGCCAATAGGTCAGTTGCTGATAAAGAGGCCATTGCAAATTTCA970.11413510301575534No Hit
GTGAACGGGATCTTGAGAAATCTATGCTATTTATATACTCTTCGCTCGTTAA970.11413510301575534No Hit
ACGCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCA940.11060515137609281No Hit
GGAGGGTGGGGAGGCTGTGGTGAGAGACGGACCGAGACCGGAGATGTTTTCA940.11060515137609281No Hit
GCTATGGGGGACACGTGGATTGCATCCAGTTTGGGGCTGTTATGAGGAATGT940.11060515137609281No Hit
AGTTAGGGGATCTAGGACTGCAGTTACTTGATCATGTGGTAAAACTATGTTT940.11060515137609281No Hit
ACCAGTGGGATGCAGACATATTTTGCTTTATTTAGTCATTTTCATATTGAAG930.10942850082953863No Hit
GGCAATGGGGATACTTTTTTATAGCTCTGCACGATATAGGATATTGGAAGTG930.10942850082953863No Hit
GTAACGGGGGGCTTGGGATTTATTAGGACACTTTTAGGGTAAAATTTATCTG920.10825185028298445No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA910.10707519973643027No Hit
CACCGTGGGATGGTATGTTTTTCTGTTTAGAAATTTTTATGTAGTCAGATTT910.10707519973643027No Hit
GCTAGAGGGATCTCAAAACAGTTATAAGATTGACAATATGATCCCTACTTTG910.10707519973643027No Hit
AAATTTGGGGAGAACAAAGAGACAACGTACCAGCATCTCTGGGACACAGCTA910.10707519973643027No Hit
TATGCAGGGAAGACGCTATTGATAACGGAATGGTTGCAATTAAGGACATTTT910.10707519973643027No Hit
TGGCACGGGGGTTGGGGATGTTGGGGAAGAGAAGTCCAGGATAATTCTAGCT900.1058985491898761No Hit
TTGTGTGGGAGAATTAACTACGGTTTGCATGACACTGAACAGCAGGAAAAGG900.1058985491898761No Hit
CAGAGGGGGATGACAGAAGAATTAAAAGAAATGAACAAAGTCATCCGGAAAT900.1058985491898761No Hit
TGCTTCGGGATACAGATATTCTACAAATTGTCTTTAAAATGATTACAACTTA900.1058985491898761No Hit
CAAGTAGGGAAATATATTAATTTTGAATTATGCCTTTGATAGCATACTTATA900.1058985491898761No Hit
GATCAGGGGGTGCCTCAATGTGGGTGTGCTTTACCTATTGCCAGATGCCTTG890.10472189864332192No Hit
ATGTTCGGGACATTTTGTGCAGTGATTATTTTTTAAAGTCTTCTTTCATGTA890.10472189864332192No Hit
TCATATGGGACTGTGGAAATCAGTCTCGTTTCTTGTCTCGCTTTGTGCTAAA890.10472189864332192No Hit
AATGGCGGGAACACAAAAAGACTAAGAGGTATACACACTCAGCTCGGTAATT890.10472189864332192No Hit
GATCACGGGATAGGAGAAAAGTAGAAAATATAGATAAATGAAAAGAAGAAAA880.10354524809676774No Hit
ACAACAGGGATCTGGTTCATTTACTGTTATATATCCTTGTGGAATAGGACAG880.10354524809676774No Hit
TCATAGGGGTTAATCAAAATGCAATGCTTTGCAAGTCTTTACTGCTTGGAGG880.10354524809676774No Hit
GAATGGGGAAAAGTTGAAAGCATTCCTCTTAAGAACTGGACTAAGACAAGGA870.10236859755021355No Hit
CTCAGTGGGGGATTGGTGTCTTTCTTTTTCTTCCTTTTTTTAAAAAAATCTA860.10119194700365938No Hit
AATGCAGGGGTATCATGGAAGAATAGAGGTTATTCTGTAATGGAAAAGTGTT850.10001529645710519No Hit
TCTTATGGGACTTCTTCCTTTTCAATGTGTCTTCTTTCTTTCTTCTTTTTCC850.10001529645710519No Hit
AATTTTGGGGTCCCCCATTTCACCTTATCCTTCTGTATCATACTCAGTAAAG850.10001529645710519No Hit
TTTCGTGGGGTTCATCGATTGAGCAATCCAATCAAAAGACAAATATTGTCAG850.10001529645710519No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)