FastQCFastQC Report
Sat 31 Dec 2022
SRR5849750.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849750.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85186
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC22542.645974690676872No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT21092.475758927523302No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA4750.5576033620547978No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA3760.44138708238442936No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC2890.33925762449228747No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC2060.2418237738595544No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT2020.23712816660014557No Hit
AACATTGGGACATAGGAATTTGAATGTGGGTCAGAATATCACTAATTTTGAT1400.1643462540793088No Hit
AAGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTC1360.1596506468199No Hit
TGATATGGGAACTACCATAGCTAATATTCCTTTCCTTTCTGCAGGTCAATTT1310.15378113774563895No Hit
TTGGGTGGGAATCAATGTTTACTTTACATGTTAAAAATAAGAAACTAAGACC1280.15025943230108232No Hit
TCGTCTGGGGCTGCCTTGTGTGTGCTGTACGTATCCTGCGTTAGGACCCTTG1250.14673772685652572No Hit
GCCTCAGCCTCCCGAGTGGCTGGGACTGCAGGCGCCCGCCACCAAGTCCGGT1250.14673772685652572No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1220.1432160214119691No Hit
GTTACTGGGGACAGCAAACTGCTGAGGTCACTTCCCCAGGCCCACTGAATGA1200.1408682177822647No Hit
AAGTGCGGGCAACAAGTGATTATTGATGATCTACAGTGTAACAGGCACGACG1200.1408682177822647No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC1200.1408682177822647No Hit
CTAACTGGGGAGGGAAGGATGCGTTCCAGGTATCTTTCCTGAGTTTATGAAA1200.1408682177822647No Hit
TATAGGGGGGCCAATAGGTCAGTTGCTGATAAAGAGGCCATTGCAAATTTCA1190.1396943159674125No Hit
GGCACTGGGGTCCTTAGGGACACACCACAAGCATGGAGGAATCAGAGTAGAG1180.13852041415256028No Hit
TGCGTTGGGCACTCTGCTCCACCAGCTTCTTGTGGAAGGCTGGGTATTATCC1170.1373465123377081No Hit
TTTACAGGGAATTACCAGATCTTCCCAATATTATAACATCCGTGCATTCCAG1160.13617261052285587No Hit
AGTTCAGGGAACCTCAAAAGTTTCAAAAGTTCTTTAACCAGATAAACTCCAT1160.13617261052285587No Hit
CCTGTTGGGACCTCAAGTGCAGGTGCAGGATGAAGAAATTACAGAGGATGAC1150.13499870870800368No Hit
TTTCGTGGGGTTCATCGATTGAGCAATCCAATCAAAAGACAAATATTGTCAG1110.13030310144859483No Hit
TATGCAGGGAAGACGCTATTGATAACGGAATGGTTGCAATTAAGGACATTTT1100.12912919963374264No Hit
TGGCACGGGGGTTGGGGATGTTGGGGAAGAGAAGTCCAGGATAATTCTAGCT1070.12560749418918601No Hit
GTTATAGGGATCTATATGTAAGTTACTAACATTATTATTTTGTCAGATGGAG1050.1232596905594816No Hit
CTTAAAGGGTCTATGTGCGGGATTCCCAGATTCTTTCCTGAATAGATTTGAA1050.1232596905594816No Hit
TCTTATGGGACTTCTTCCTTTTCAATGTGTCTTCTTTCTTTCTTCTTTTTCC1040.12208578874462941No Hit
GATGGGGGAGACGCCAGAGGTGCAGCTCCAGCAGCAATGGCAGTGACGGCGT1030.1209118869297772No Hit
TCTAGAGGGAGGGCTTGAACTCCTGGGCTCAAGCAATCTGTCTGCCTCGGCC1020.11973798511492499No Hit
ACCAGTGGGATGCAGACATATTTTGCTTTATTTAGTCATTTTCATATTGAAG1000.11739018148522057No Hit
TAACGTTGGGGGTGAACATGACGGCAGCCGTGCGGACCTACCGCTGGCAGTG1000.11739018148522057No Hit
GCCAAGGGGCGATCTTTTGGAGGGGGCTCACTGCTTCCTTGAACTCCTGAGC990.11621627967036838No Hit
GCAGTGGGTATAAAGCACCAGACAGAAGAGTCTGGGTCATGTTATGCTACCA980.11504237785551616No Hit
GTGAACGGGATCTTGAGAAATCTATGCTATTTATATACTCTTCGCTCGTTAA980.11504237785551616No Hit
AAATCAGGGGGAAGGTTTATGCTCTGAGCTCAGGATAGTTGGGGAACACATT970.11386847604066397No Hit
TCCGTAGGGATTTGAAATTTGAAGAAACTCGTATGTTCCATGTGTTTTGCCA970.11386847604066397No Hit
AAGACTGGGATATTCTAGATATGAACACTTGGCATTTGACATTTTCTAAAGC970.11386847604066397No Hit
TGCAACGGGGACTGATAGAGTTCTGTCTCCTTTCTCTTTTGCTACCCAACCT950.11152067241095955No Hit
GCTCATGGGCAGGAGGAGAATGACTACTAATCTGGAAGAAAGGTCTTACTAT940.11034677059610734No Hit
GATCACGGGATAGGAGAAAAGTAGAAAATATAGATAAATGAAAAGAAGAAAA940.11034677059610734No Hit
GTAGTGGGGACTCTGCTATTGGATATATCCACAGTCTGATGGGCAACTTTGA940.11034677059610734No Hit
AGCTTTGGGATATTTATGTAGAATCAAATTATATAAACAGTACCAATATCAT940.11034677059610734No Hit
CAAGTAGGGAAATATATTAATTTTGAATTATGCCTTTGATAGCATACTTATA940.11034677059610734No Hit
AAATTTGGGGAGAACAAAGAGACAACGTACCAGCATCTCTGGGACACAGCTA930.10917286878125514No Hit
TAAGTCGGGGCATTTTGTTCAACTAAATTAATATTTGTAGATTGCAAGTTTT920.10799896696640293No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT920.10799896696640293No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTA910.10682506515155073No Hit
AATTTTGGGGTCCCCCATTTCACCTTATCCTTCTGTATCATACTCAGTAAAG910.10682506515155073No Hit
GTAACGGGGGGCTTGGGATTTATTAGGACACTTTTAGGGTAAAATTTATCTG910.10682506515155073No Hit
TGCTTCGGGATACAGATATTCTACAAATTGTCTTTAAAATGATTACAACTTA900.10565116333669852No Hit
ATACTAGGGGATTGAGAGTAAGAAAATATGAAGCAATTGATGATCTGCACAA890.10447726152184632No Hit
GTTGACGGGACAGAACATGGTTGTTATTGTGCTGTTGACATGAATAGACCAT890.10447726152184632No Hit
ACGGCGGGACATATGGATTTGGGAGAATGGCTTTTTATGTCCCTGAATTCTT890.10447726152184632No Hit
GATCAAGGGGGCTCTGCTGCTGCGGAGCCCCCGCCTGCCCGCCCGGGAGCTG880.1033033597069941No Hit
GGAGGGTGGGGAGGCTGTGGTGAGAGACGGACCGAGACCGGAGATGTTTTCA880.1033033597069941No Hit
GGCAATGGGGATACTTTTTTATAGCTCTGCACGATATAGGATATTGGAAGTG880.1033033597069941No Hit
CAGAGGGGGATGACAGAAGAATTAAAAGAAATGAACAAAGTCATCCGGAAAT870.10212945789214191No Hit
TAGCTTGGGGTGTAGTACACCTGCATGATCAAATGCTATAATTTACTATAAT870.10212945789214191No Hit
GCTATGGGGGACACGTGGATTGCATCCAGTTTGGGGCTGTTATGAGGAATGT870.10212945789214191No Hit
TAGCCTGGGCTTTGTACAACAGTACAATTATTATCTGAATTTTACAGATTTT860.10095555607728969No Hit
GTTTTAGGGAATATTTATTTGCATAGTCTATTTATTGTATTCGTATCACACT860.10095555607728969No Hit
GCTAGAGGGATCTCAAAACAGTTATAAGATTGACAATATGATCCCTACTTTG860.10095555607728969No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)