FastQCFastQC Report
Sat 31 Dec 2022
SRR5849746.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849746.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92073
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC23412.542547761015716No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT20522.228666384282037No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA4260.462676354631651No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA2900.314967471462861No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC2540.27586806121229895No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT2170.23568255623255457No Hit
GTTAGCGGGATATGTGTGTACATATATATGTACATGTATATGTGTGTACATA1890.2052719038154508No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC1720.18680829341935204No Hit
ATCACCGGGGTACATTGTTAGTGGGAATGAAAATTAGTACATTATTGTTGTA1570.17051687248161784No Hit
ACAATTCGGGATGCTCAAGTTATATAAAAACTCATTGTTTTTATTCTAGCTG1520.16508639883570644No Hit
TCATCAGGGAGGCTAGGTACCTCAAAGAAGTGGAATCATGTAGTAGTGTTTC1510.16400030410652416No Hit
CTATGATGGGAGGAGCAAGCAGCTGATTGCCCCAGTGGATATCTAAGAAAGG1480.16074201991897735No Hit
CGCGCAGGGATAGGAGTTAATAAAATCCAGCGTCAATACTTCATGATTCACA1460.1585698304606128No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1450.1574837357314305No Hit
TATAAAGGGCGATCTACACTAGGGAGTGCAGCTGCCGCTACCGCCGCCCTCT1360.14770888316879No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC1350.14662278843960772No Hit
CGTAACGGGAGTTACCCGTAGTAGAGCTATTTCTTGTACTTCTAAGTTTTCT1290.14010622006451404No Hit
TAAGGCTGGGGTTAAGCTATTACTGGTAAATAATTTGTAATTTTAGTGCTTC1290.14010622006451404No Hit
TTGTTTGGGAAAGTATTCATGAATGCATAAAATATATACAGATACTAGTCTA1290.14010622006451404No Hit
ACAGTTGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1260.1368479358769672No Hit
GTTACGGGGCAGATCAAGTGGAATTTGTGGAACATCTTATTTCACGAATGTG1240.13467574641860264No Hit
GTAAAAGGGGAGTATCTAAATAGACAAGGATGAGGTGAATGAGAAGACATGG1230.13358965168942036No Hit
GCGATGGGCAGTATATACCAAAGCTTTGACTTGTTTGAGCTCTTGAGCTTAG1230.13358965168942036No Hit
GTGTCCGGGATCCTCTCCAACCTTTACATTCACAAAAACCATCCTTGTGTAC1160.1259869885851444No Hit
AATTGCGGGGGCTCGGTGGGTACTGTCGCGGAGGCTTGTCATTCTGACCCGG1140.12381479912677983No Hit
AGCTCCGGGAGGTGGCCTAGGAATGTATCTAGGAGTGTGGAGATTGTAAAAT1130.12272870439759755No Hit
TATTTAGGGAACATTTGCTTTATTATAGAACAATAAACATGCAGTGATTGAT1120.12164260966841527No Hit
TCCATGGGGACCATTGTGAAATCCTTTCTCAGTGTAACTGCATTTCTAATAA1120.12164260966841527No Hit
TTAGTTGGGAGAGAAAGTGAGCAAACTGGTTTTGGTAATAATTGACATACCT1120.12164260966841527No Hit
CACTCTGGGGTTCGCCAGGACCTTGCAGATGTCGTCCAGGTGTGCGAAGGAA1120.12164260966841527No Hit
AGCTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1100.1194704202100507No Hit
ACAAAAGGGACTCTGTCAATATGACAATGCCATTCACACTGGTGCTCCGACC1050.11403994656413934No Hit
TTGTTAGGGTGGAGGAATTATGCTGGGCCCTGCCTTGGTCTGGTCACCAGGG1040.11295385183495706No Hit
ACCAAGGGGATTGCTTAAAAAATGTATAAAGCAGCTGGAAATGTTTTAAATA1040.11295385183495706No Hit
GCGCTGGGGAGGGAACCAAAGTTGCACTATCTGGGCCCAGATTGTCTGGTTG1040.11295385183495706No Hit
TTGTGCCGGGATCCAAACAGTAATAGGAGTATTTTTTTCAAGTTATTCAGTG1040.11295385183495706No Hit
CGGGCCGGGATAGACAGACACAAAAATTCTGTCAGAGGTAAGCCAGATCCAG1040.11295385183495706No Hit
GTAAACGGGACAAGTGCTGAGAGAACTGTCAACCTATTATAGAATTCAGACA1010.10969556764741022No Hit
CCGTATGGGATTTCATCACAACAATTATCAATATAAAACCCCCTGCCATAAC1000.10860947291822794No Hit
GACTTAGGGAAACATTGCTAATGTAGCACTTTCCAATTGTGTTTATATTTCC1000.10860947291822794No Hit
GGTAATGGGAATAAGCAAAATGCAAATTAAACTATAATGAGATACCATTTCA990.10752337818904566No Hit
TACATAGGGTCCCCCATGGGTGACTGATTAAACAAACTGATACATCCATCTT970.1053511887306811No Hit
AGCGATGGGCACCTCTGGGTGACCTTGAGTTTCTGCATAGGTAGGAAGGAAA960.10426509400149882No Hit
TTGCGGGTGGTCCTGCCGATGTGGAAGACACTACAGGAAGTGGAGCTACAGA960.10426509400149882No Hit
TTTCAAGGGTATTCCATGTTGACTTAGATATTAAGAGAATACTATACTTTCC950.10317899927231654No Hit
ACTATAGGGGTTCATTCAATTCCTACCTTGTTGTAAGCACTGGGCTAGTCGC940.10209290454313426No Hit
CATTCCGGGCGTGGACCAAAGGAAATGTGAACGTCTTCTGCTTTACCTCTAT940.10209290454313426No Hit
TTGTTAGGGAACACTGGGTGGAAGTTTTTATGGTAACAGTGGTCTGAGGCCC930.10100680981395198No Hit
TTCATAGGGATTCTATACAAAAGTAAACCTCTCTAGTGTTTGATTTTAATAG930.10100680981395198No Hit
GACTATGGGAGGACCACAGGCTCATGCCTCCACATCCTGCTACATTTTTTAT930.10100680981395198No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)