FastQCFastQC Report
Sat 31 Dec 2022
SRR5849745.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849745.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90144
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC23672.6257987220447285No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT20032.2220003549875753No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA4350.4825612353567625No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA2920.32392616258430956No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC2670.29619275825346114No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT2140.23739794107206247No Hit
ATCACCGGGGTACATTGTTAGTGGGAATGAAAATTAGTACATTATTGTTGTA1660.1841498047568335No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1640.18193113241036563No Hit
ACAATTCGGGATGCTCAAGTTATATAAAAACTCATTGTTTTTATTCTAGCTG1580.17527511537096202No Hit
GTTAGCGGGATATGTGTGTACATATATATGTACATGTATATGTGTGTACATA1580.17527511537096202No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC1570.17416577919772808No Hit
TTAGTTGGGAGAGAAAGTGAGCAAACTGGTTTTGGTAATAATTGACATACCT1550.1719471068512602No Hit
CGCGCAGGGATAGGAGTTAATAAAATCCAGCGTCAATACTTCATGATTCACA1510.16750976215832447No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC1380.1530883919062833No Hit
TATAAAGGGCGATCTACACTAGGGAGTGCAGCTGCCGCTACCGCCGCCCTCT1300.1442137025204118No Hit
GTAAAAGGGGAGTATCTAAATAGACAAGGATGAGGTGAATGAGAAGACATGG1290.14310436634717785No Hit
TCATCAGGGAGGCTAGGTACCTCAAAGAAGTGGAATCATGTAGTAGTGTTTC1290.14310436634717785No Hit
TAAGGCTGGGGTTAAGCTATTACTGGTAAATAATTTGTAATTTTAGTGCTTC1280.1419950301739439No Hit
CTATGATGGGAGGAGCAAGCAGCTGATTGCCCCAGTGGATATCTAAGAAAGG1250.1386670216542421No Hit
TTGTGCCGGGATCCAAACAGTAATAGGAGTATTTTTTTCAAGTTATTCAGTG1230.13644834930777425No Hit
GTTACGGGGCAGATCAAGTGGAATTTGTGGAACATCTTATTTCACGAATGTG1220.1353390131345403No Hit
CACTCTGGGGTTCGCCAGGACCTTGCAGATGTCGTCCAGGTGTGCGAAGGAA1210.13422967696130636No Hit
CGTAACGGGAGTTACCCGTAGTAGAGCTATTTCTTGTACTTCTAAGTTTTCT1200.13312034078807242No Hit
CTGTTTGGGATTAATGGATTCTTCCTTGACAACAAGTCCTTGATGGTGGAGC1170.1297923322683706No Hit
GCGATGGGCAGTATATACCAAAGCTTTGACTTGTTTGAGCTCTTGAGCTTAG1170.1297923322683706No Hit
TACATAGGGTCCCCCATGGGTGACTGATTAAACAAACTGATACATCCATCTT1170.1297923322683706No Hit
ACAGTTGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1170.1297923322683706No Hit
AGCTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1130.12535498757543487No Hit
TATTTAGGGAACATTTGCTTTATTATAGAACAATAAACATGCAGTGATTGAT1120.12424565140220092No Hit
GCATTAGGGGGAATGCAGAGGGGAGAAAAGGTTGAAAAACATCTGAACAGAA1110.123136315228967No Hit
GACTTAGGGAAACATTGCTAATGTAGCACTTTCCAATTGTGTTTATATTTCC1100.12202697905573305No Hit
GAAATTGGGCTTCCGATCTAGAAACGGGCAACAAAACTGGCAAATTCTTAGA1080.11980830670926518No Hit
TTGCGGGTGGTCCTGCCGATGTGGAAGACACTACAGGAAGTGGAGCTACAGA1060.11758963436279729No Hit
TCCATGGGGACCATTGTGAAATCCTTTCTCAGTGTAACTGCATTTCTAATAA1060.11758963436279729No Hit
TTGTTAGGGAACACTGGGTGGAAGTTTTTATGGTAACAGTGGTCTGAGGCCC1050.11648029818956337No Hit
CGTCAAGGGCCCTCAAATCCTAGACATCATCTCGCTGCCTCCATAAATCCTT1050.11648029818956337No Hit
CAACGCGGGGTTGTAGCAAGGCCCTCTGTAGCCTGAGATCTGCTTTTTTCTA1040.11537096201632943No Hit
CGCTCCGGGATCCTTGGTGGCCCTTAATCCAGAATGCATGCCATGATTACAC1040.11537096201632943No Hit
GTTTCAGGGACAGCTTTTCTGTTGGTATGCATAATTAATAATTGGAGCTGCA1030.1142616258430955No Hit
CCTGTAGGGAAATGCTCTTTTGCAAAAAAGTATGATTAAAGGATTTCAGCGT1030.1142616258430955No Hit
GGTAATGGGAATAAGCAAAATGCAAATTAAACTATAATGAGATACCATTTCA1030.1142616258430955No Hit
CTTATGGGTCTCTTTTTGTATTGGAATCTAGCTGAATTGCATGTGTCTAGAG1030.1142616258430955No Hit
AATATAGGGATAAACGTCTTGTACCCGGAGGTGGAGCAACAGAAATTGAATT1010.11204295349662761No Hit
CATTCCGGGCGTGGACCAAAGGAAATGTGAACGTCTTCTGCTTTACCTCTAT1000.11093361732339369No Hit
GCAAATGGGATAGTTCAAGATTTTCGGTATATAAAATCTGTCCTTTCCTACC990.10982428115015974No Hit
AATTGCGGGGGCTCGGTGGGTACTGTCGCGGAGGCTTGTCATTCTGACCCGG980.1087149449769258No Hit
GTGTCCGGGATCCTCTCCAACCTTTACATTCACAAAAACCATCCTTGTGTAC970.10760560880369185No Hit
AGCGATGGGCACCTCTGGGTGACCTTGAGTTTCTGCATAGGTAGGAAGGAAA950.10538693645722401No Hit
ACCAAGGGGATTGCTTAAAAAATGTATAAAGCAGCTGGAAATGTTTTAAATA950.10538693645722401No Hit
TTTTGTGGGATTTTTGATGATAGAGAATAAATAGGGTTTTTGAAACCTTTGT950.10538693645722401No Hit
GCGCTGGGGAGGGAACCAAAGTTGCACTATCTGGGCCCAGATTGTCTGGTTG940.10427760028399007No Hit
GCATCTGGGCTAATTTCTGGTTGTAATGAATTTATTACATTAATATTACACT940.10427760028399007No Hit
ACTATAGGGGTTCATTCAATTCCTACCTTGTTGTAAGCACTGGGCTAGTCGC940.10427760028399007No Hit
TTGTTTGGGAAAGTATTCATGAATGCATAAAATATATACAGATACTAGTCTA940.10427760028399007No Hit
TTCACAAGGGCGGATAGTTGTATACAAATGAGCAAGGTATTTGTTGCAACTT940.10427760028399007No Hit
GGCATAGGGAGGTTAAAAAGGGAAGGTTTGGGAGGCTCTCTTGGCCTATGAA920.10205892793752219No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)