FastQCFastQC Report
Sat 31 Dec 2022
SRR5849744.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849744.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89617
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC21792.4314583170603794No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT19172.139103071961793No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA4330.48316725621254897No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA2750.30686142138210387No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC2480.27673320910095184No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC2400.2678063313880179No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT1960.2187085039668813No Hit
GTTAGCGGGATATGTGTGTACATATATATGTACATGTATATGTGTGTACATA1700.189696151399846No Hit
CGCGCAGGGATAGGAGTTAATAAAATCCAGCGTCAATACTTCATGATTCACA1690.1885802916857293No Hit
ACAATTCGGGATGCTCAAGTTATATAAAAACTCATTGTTTTTATTCTAGCTG1580.17630583483044512No Hit
TCATCAGGGAGGCTAGGTACCTCAAAGAAGTGGAATCATGTAGTAGTGTTTC1530.1707265362598614No Hit
CTATGATGGGAGGAGCAAGCAGCTGATTGCCCCAGTGGATATCTAAGAAAGG1490.16626309740339446No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1480.1651472376892777No Hit
TATAAAGGGCGATCTACACTAGGGAGTGCAGCTGCCGCTACCGCCGCCCTCT1460.16291551826104422No Hit
TTCACAAGGGCGGATAGTTGTATACAAATGAGCAAGGTATTTGTTGCAACTT1430.15956793911869402No Hit
ATCACCGGGGTACATTGTTAGTGGGAATGAAAATTAGTACATTATTGTTGTA1360.15175692111987682No Hit
TTGTTTGGGAAAGTATTCATGAATGCATAAAATATATACAGATACTAGTCTA1360.15175692111987682No Hit
AGCTCCGGGAGGTGGCCTAGGAATGTATCTAGGAGTGTGGAGATTGTAAAAT1320.14729348226340983No Hit
GCGCTGGGGAGGGAACCAAAGTTGCACTATCTGGGCCCAGATTGTCTGGTTG1290.14394590312105962No Hit
CATTCCGGGCGTGGACCAAAGGAAATGTGAACGTCTTCTGCTTTACCTCTAT1260.1405983239787094No Hit
GCGATGGGCAGTATATACCAAAGCTTTGACTTGTTTGAGCTCTTGAGCTTAG1250.13948246426459265No Hit
GTTTCAGGGACAGCTTTTCTGTTGGTATGCATAATTAATAATTGGAGCTGCA1210.1350190254081257No Hit
GGTAATGGGAATAAGCAAAATGCAAATTAAACTATAATGAGATACCATTTCA1210.1350190254081257No Hit
AATTGCGGGGGCTCGGTGGGTACTGTCGCGGAGGCTTGTCATTCTGACCCGG1200.13390316569400895No Hit
TTAGTTGGGAGAGAAAGTGAGCAAACTGGTTTTGGTAATAATTGACATACCT1200.13390316569400895No Hit
TTGTGCCGGGATCCAAACAGTAATAGGAGTATTTTTTTCAAGTTATTCAGTG1190.13278730597989222No Hit
CACTCTGGGGTTCGCCAGGACCTTGCAGATGTCGTCCAGGTGTGCGAAGGAA1180.13167144626577545No Hit
TCTCAGGGGAGGATACTATATCACTATTAGTTTGGACTTCAACTAGTCTATA1170.13055558655165875No Hit
GACTTAGGGAAACATTGCTAATGTAGCACTTTCCAATTGTGTTTATATTTCC1170.13055558655165875No Hit
TTGTTAGGGAACACTGGGTGGAAGTTTTTATGGTAACAGTGGTCTGAGGCCC1160.12943972683754199No Hit
TAAGGCTGGGGTTAAGCTATTACTGGTAAATAATTTGTAATTTTAGTGCTTC1140.12720800740930852No Hit
GAAATTGGGCTTCCGATCTAGAAACGGGCAACAAAACTGGCAAATTCTTAGA1090.1216287088387248No Hit
TTGTTAGGGTGGAGGAATTATGCTGGGCCCTGCCTTGGTCTGGTCACCAGGG1070.11939698941049132No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC1070.11939698941049132No Hit
CTGTTTGGGATTAATGGATTCTTCCTTGACAACAAGTCCTTGATGGTGGAGC1060.11828112969637457No Hit
ACAGTTGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1060.11828112969637457No Hit
TTGCGGGTGGTCCTGCCGATGTGGAAGACACTACAGGAAGTGGAGCTACAGA1040.11604941026814108No Hit
GCATCTGGGCTAATTTCTGGTTGTAATGAATTTATTACATTAATATTACACT1030.11493355055402434No Hit
GCATTAGGGGGAATGCAGAGGGGAGAAAAGGTTGAAAAACATCTGAACAGAA1020.11381769083990761No Hit
TATTTAGGGAACATTTGCTTTATTATAGAACAATAAACATGCAGTGATTGAT1020.11381769083990761No Hit
GGTCATGGGCTGGAGGAGGAGTAATGAATGGTGGGAGGACAGTGAAAAAGGG1020.11381769083990761No Hit
GTAAAAGGGGAGTATCTAAATAGACAAGGATGAGGTGAATGAGAAGACATGG1020.11381769083990761No Hit
CCTGTAGGGAAATGCTCTTTTGCAAAAAAGTATGATTAAAGGATTTCAGCGT1010.11270183112579085No Hit
AAGCAGGGCCAGAGATTTTGTGTCTATGGTTGTGGGTCTGGAAACCTGGCAG1010.11270183112579085No Hit
TTTCAAGGGTATTCCATGTTGACTTAGATATTAAGAGAATACTATACTTTCC1010.11270183112579085No Hit
CGTAACGGGAGTTACCCGTAGTAGAGCTATTTCTTGTACTTCTAAGTTTTCT1000.11158597141167413No Hit
AGCGATGGGCACCTCTGGGTGACCTTGAGTTTCTGCATAGGTAGGAAGGAAA990.11047011169755738No Hit
GTAAACGGGACAAGTGCTGAGAGAACTGTCAACCTATTATAGAATTCAGACA990.11047011169755738No Hit
GTTACGGGGCAGATCAAGTGGAATTTGTGGAACATCTTATTTCACGAATGTG980.10935425198344065No Hit
CTCACTGGGATTACACAAGAAACTTGGTCTGCAGTCTGGAAGCTTGTCTGCT980.10935425198344065No Hit
CAACGCGGGGTTGTAGCAAGGCCCTCTGTAGCCTGAGATCTGCTTTTTTCTA980.10935425198344065No Hit
CGCTCCGGGATCCTTGGTGGCCCTTAATCCAGAATGCATGCCATGATTACAC980.10935425198344065No Hit
TTTGCAGGGGGGCAGCAGTGTGTGGAGTCTCGCTTCGTGAACTTTTGCTCTA970.1082383922693239No Hit
CCGTATGGGATTTCATCACAACAATTATCAATATAAAACCCCCTGCCATAAC960.10712253255520715No Hit
TATGCAGGGACGTGGTAAAAAAGACATGAATAAAAATACAACAAATTTAGAA950.10600667284109042No Hit
ACCAAGGGGATTGCTTAAAAAATGTATAAAGCAGCTGGAAATGTTTTAAATA950.10600667284109042No Hit
CGTCAAGGGCCCTCAAATCCTAGACATCATCTCGCTGCCTCCATAAATCCTT950.10600667284109042No Hit
TTAAGATGGGATGGTATATTTAGTAATTCTCTTATAAATATAACTATGAGAA940.10489081312697367No Hit
AATATAGGGATAAACGTCTTGTACCCGGAGGTGGAGCAACAGAAATTGAATT940.10489081312697367No Hit
AAATTGGGGATTCTTCACTGTGAAGACCTAGTCCAACATATCAATATCCACA930.10377495341285695No Hit
GGAGCAGGGAAAGCAGCTTTTTGAGACAAGAATTAAGATTGTTGCCCTGATG910.10154323398462346No Hit
ACAAAAGGGACTCTGTCAATATGACAATGCCATTCACACTGGTGCTCCGACC910.10154323398462346No Hit
CGTTTAGGGTCAAGCGGGGTGTGTCAACATTCCCTATAGGACAAAGTCTTAT900.10042737427050671No Hit
GTACAAGGGAGTAACCATCTTTCCACTAGTTCATAGACTGAGAAACAGTAAA900.10042737427050671No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)