FastQCFastQC Report
Sat 31 Dec 2022
SRR5849739.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849739.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47441
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC14212.9952994245483864No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT13522.849855610126262No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA3390.7145717839000021No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT2900.6112855968466095No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA1980.4173605109504437No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC1590.33515313758141696No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT1360.28667186610737544No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT1250.26348517105457303No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC1060.22343542505427794No Hit
ACACGACGGGGTATTATTTTAATTGACTATTTTTCTGTTTAAACAAAATAAA970.20446449273834869No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT940.1981408486330389No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCG920.19392508589616578No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC920.19392508589616578No Hit
GCAAATATTGTCCCACCCTGACAATATGCTTTTCTTCTCTGAAACCTTTCTT890.187601441790856No Hit
TGCTACGGGCTTTCATGTACTGAAGGGTCAAAGTTTCAATGTAGATCTTTAA760.1601989840011804No Hit
TCGCGCTGGGAAGGCATCAGTTGCTGGCTTTTCTTTGCAATTTTATCCTCTG760.1601989840011804No Hit
GTTATTGGGATGTTTCTCTGTCGGAGGATAAATAACAACGACAATTCCTGAA760.1601989840011804No Hit
CGATGAGGGATCTTGGACCGGGATTTGAATATAAGCTTTTTGAGGTCAGATA740.15598322126430725No Hit
CCGAGAGGGGGGTCAAAGCTGTGGGTTATCAGAGTGTCATCTGGGAAATTAG730.15387533989587066No Hit
ATGCGCGGGTAGGCCTAGGCTCATATGTGTGTTTGTGTCTTTGTTTTTAACA710.1496595771589975No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCT710.1496595771589975No Hit
AATTATGGGATTCTTACCGTAGTTCAGGCCCAAAACATGCCCATCTCATCTA670.14122805168525115No Hit
GTGAACGGGAGTTCTACAAGGTCACATTTATGTGGAAGTCCAAAGTCAAGTC650.137012288948378No Hit
TTGTGCGGGAGACTGCTTTCCACAGGGACTGAACTAATTTACAGTCCCATCA630.1327965262115048No Hit
GGCACGGGGGGTTGAGGCTGGCTGTCAGCCAGAATGCCTAAAGTCTGGCCAA620.13068864484306822No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATG610.12858076347463165No Hit
CCCTGATGAACCTAGATGCAAAAATCCTTAACAAAATACTAGCAAACCAAAC590.12436500073775848No Hit
TCTTCAGGGGTTAGTAGAATTCTTGTGGAAAATAACATGGTTTAGTAAGTGA590.12436500073775848No Hit
TCTACGGGAGCGCTTTTGAACCCAAAAGACAAGCTCCCTTCTTCCTGCGTTG570.12014923800088531No Hit
GGTGTCGGGGCAAGAGCCCTGATGCAACAATGAAAACAGAGAGCTTGACATA570.12014923800088531No Hit
TATGCCGGGATGTTTACTCAGGGTTAGAGCTGAGCCCTACCTAAGCTCAGAA560.11804135663244872No Hit
CAGTTGGACACCTCCAGTCCAGACCCCCACCTAACCTTCTAGCTAGTCCCTA560.11804135663244872No Hit
ACGTTAGGGGAGTTCTGTATGCTTTCTGTAAAGTCTCTGAATTAGTTCCGAA540.11382559389557555No Hit
GGTCAGGGATGTATGGATATTTCTGCTACGGATTTCTTAATTCCTTCCTAAC530.11171771252713897No Hit
ATCATAGGGACAACTGGGCTTATCACTGGGAAAACACAAAAAATTACACAAC530.11171771252713897No Hit
ATGGTTGGGGTTTCTATCTTGATTATTTGTATTTTATAGCTTTGTCAAACTG520.1096098311587024No Hit
TCACACGGGATAACTAAAAAGAAATAGTCTCTCACAAGGCAGAAAAGTTGGA520.1096098311587024No Hit
GTAGAGGGATATTTTAAAGTCTAGTTTATATTATACGGAACAATGCAGAGAA510.10750194979026581No Hit
TATAAAGGGAAGTCCCTTATGTAAATATACATATTTTCATATTACCTTTGCA510.10750194979026581No Hit
CGAACTGGGGGAAAGATAGTCTTTTCCAAAACAAATGCTGAAAGAATTTGCC510.10750194979026581No Hit
CTGGCCAGATTATAGTAGCATCCCCTGCTCCCAATTGTGACAAACAAGCAAG500.10539406842182922No Hit
TCTGTCGGGTGCTAATGCAGGAGCATGCTGATGCTGTCACCTAGAGAAACAA500.10539406842182922No Hit
TCGCCTTTGGCAAACGTGATAACATAGTCATGGGAGTGACATCCCGTCACCT500.10539406842182922No Hit
ATTCAAGGACAGAAACTGGCCATGGTATAATGGAAGAACCAGAACTTACTTT500.10539406842182922No Hit
GTCATCGGGAACCGGGGAGGATGAGGAACGGCATCTCTGTTTTGAGTAAACA490.10328618705339264No Hit
GATGCTGGGGTTCGTGGCCTAGTGCCTTGGATTTTACATATTTTTCTTTTTA490.10328618705339264No Hit
TGGCTGGGGTCAGTTTCTAAGCTGACTAAACAACTAATGCTCCTGGAGGCAA490.10328618705339264No Hit
ACAATAGGGGAGCTTTCCAAGTTCACAGATATGAAATTTGGTCACTTACCCA480.10117830568495605No Hit
GCTATTGGGATCAGCAAAATATGACATGGCAGAAGACCTGTTAAAACGGTGC480.10117830568495605No Hit
GGTCACGGGGTCGCTGAAACTGGCTTAATCAGAGAGGGAATTTATTACCACA480.10117830568495605No Hit
TCTACGGGGAGTTAGCCAAGGTGGAGGATGGGAAAAGAATGGAACTGTCCCA480.10117830568495605No Hit
GTTAATGGGATCATGGTATATTATAGTGAGGAGCACATAGTCATTATTATTT480.10117830568495605No Hit
CATTGAGGGTATAAGCTAAAATGTCCAATTTTATAATTGAGAATCAAAAAGA480.10117830568495605No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)