FastQCFastQC Report
Sat 31 Dec 2022
SRR5849738.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849738.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47549
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC13522.843382615827883No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT13182.771877431702034No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA3380.7108456539569707No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT2970.6246188142757997No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA2070.43534038570737554No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC1960.4122063555490126No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT1570.33018570316936213No Hit
GCAAATATTGTCCCACCCTGACAATATGCTTTTCTTCTCTGAAACCTTTCTT1130.23764958253591034No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC1120.23554648888515003No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT1040.21872173967906794No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC1020.21451555237754738No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1020.21451555237754738No Hit
TCGCGCTGGGAAGGCATCAGTTGCTGGCTTTTCTTTGCAATTTTATCCTCTG940.1976908031714652No Hit
ACACGACGGGGTATTATTTTAATTGACTATTTTTCTGTTTAAACAAAATAAA930.19558770952070498No Hit
AATTATGGGATTCTTACCGTAGTTCAGGCCCAAAACATGCCCATCTCATCTA900.18927842856842414No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCT830.17455677301310227No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCG810.17035058571158174No Hit
GTGAACGGGAGTTCTACAAGGTCACATTTATGTGGAAGTCCAAAGTCAAGTC800.16824749206082146No Hit
TCTTCAGGGGTTAGTAGAATTCTTGTGGAAAATAACATGGTTTAGTAAGTGA750.15773202380702012No Hit
CGATGAGGGATCTTGGACCGGGATTTGAATATAAGCTTTTTGAGGTCAGATA710.14931964920397905No Hit
CCGAGAGGGGGGTCAAAGCTGTGGGTTATCAGAGTGTCATCTGGGAAATTAG690.14511346190245852No Hit
TTGTGCGGGAGACTGCTTTCCACAGGGACTGAACTAATTTACAGTCCCATCA640.13459799364865718No Hit
CTGGCCAGATTATAGTAGCATCCCCTGCTCCCAATTGTGACAAACAAGCAAG630.1324948999978969No Hit
TCTGTCGGGTGCTAATGCAGGAGCATGCTGATGCTGTCACCTAGAGAAACAA630.1324948999978969No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATG630.1324948999978969No Hit
CATTGAGGGTATAAGCTAAAATGTCCAATTTTATAATTGAGAATCAAAAAGA630.1324948999978969No Hit
GTTATTGGGATGTTTCTCTGTCGGAGGATAAATAACAACGACAATTCCTGAA590.12408252539485583No Hit
GTCATCGGGAACCGGGGAGGATGAGGAACGGCATCTCTGTTTTGAGTAAACA560.11777324444257502No Hit
GTTAATGGGATCATGGTATATTATAGTGAGGAGCACATAGTCATTATTATTT560.11777324444257502No Hit
TTTATCGGGCAAATGACAAATGTGCTTTAAATACCTGCCATGTTCTCAGCAC550.11567015079181477No Hit
ATGCGCGGGTAGGCCTAGGCTCATATGTGTGTTTGTGTCTTTGTTTTTAACA550.11567015079181477No Hit
CGAACTGGGGGAAAGATAGTCTTTTCCAAAACAAATGCTGAAAGAATTTGCC550.11567015079181477No Hit
TCACACGGGATAACTAAAAAGAAATAGTCTCTCACAAGGCAGAAAAGTTGGA550.11567015079181477No Hit
TGCTACGGGCTTTCATGTACTGAAGGGTCAAAGTTTCAATGTAGATCTTTAA540.11356705714105449No Hit
TCTGCTGGGGGGCAGCAGTGGGGATGATCACTAGAAAGTTCTATTCAGCCAT540.11356705714105449No Hit
ATACGTGGGGAATGACACTGTATGGCAGACACACCTGAATGCATGTTCCAAG530.11146396349029422No Hit
GTAGAGGGATATTTTAAAGTCTAGTTTATATTATACGGAACAATGCAGAGAA530.11146396349029422No Hit
GGCACGGGGGGTTGAGGCTGGCTGTCAGCCAGAATGCCTAAAGTCTGGCCAA530.11146396349029422No Hit
TATGCCGGGATGTTTACTCAGGGTTAGAGCTGAGCCCTACCTAAGCTCAGAA520.10936086983953397No Hit
GGTGTCGGGGCAAGAGCCCTGATGCAACAATGAAAACAGAGAGCTTGACATA520.10936086983953397No Hit
AGAACGGGATTTCTCCAAGGATTTTCTTTTTCCCAAGATATGGCTTTTTGAG510.10725777618877369No Hit
GTAAATGGGAAGTAGTATATATGTTAGGATTACCCTCTGGTAATTGATACAG510.10725777618877369No Hit
ACAATAGGGGAGCTTTCCAAGTTCACAGATATGAAATTTGGTCACTTACCCA500.10515468253801342No Hit
TTAGTTGGGTCTACAGTTTGAAACTCTGATGCTATATATACATGGTATAATG500.10515468253801342No Hit
CACCTAGGGATAGAGGAGAAGCTTACCCCTCTTCCAGAAGATAATAGTATGA500.10515468253801342No Hit
GCTATTGGGATCAGCAAAATATGACATGGCAGAAGACCTGTTAAAACGGTGC490.10305158888725315No Hit
ACGTTAGGGGAGTTCTGTATGCTTTCTGTAAAGTCTCTGAATTAGTTCCGAA490.10305158888725315No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTC480.10094849523649288No Hit
CGACAAGGGGTGAATAGACCTAGTATTTGCCAGCACAATGAGGTGACCATAG480.10094849523649288No Hit
CCCTGATGAACCTAGATGCAAAAATCCTTAACAAAATACTAGCAAACCAAAC480.10094849523649288No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)