FastQCFastQC Report
Sat 31 Dec 2022
SRR5849737.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849737.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117691
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC22401.903289121513115No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT21761.8489094323270259No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA4760.40444893832153694No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA4070.34582083591778473No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC2790.2370614575456067No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT2100.17843335514185452No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC1700.1444460494005489No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1530.130001444460494No Hit
CATTGGACATTTTTCTTGCATTGCCTCAGTAACAGAATAGAGGAGATATATT1490.12660271388636343No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC1430.1215046180251676No Hit
GTTATCGAGGGGAGCCAGGGACCTTTTAACAACCAGCTGTCATGTGAACTCA1320.11215810894630855No Hit
AGCGAAGGGGGGAACTATCAGCATAGAAATGGAAATTAAAACACACTACTAA1290.10960906101571063No Hit
GTCACTGGGATGAAATGAGAGGATGCTTTTGAAAACACTTTAACATGATGTT1230.10451096515451479No Hit
TGTATAGGGAACGTTCTTGTGAATTTAGAATGTGTGAATAGCTCTCTAAGAT1230.10451096515451479No Hit
TGTGCATGGGACAAGCAACTGCTGAATGAATGGTAGTTATAGCCTCCTCCTC1180.10026255193685159No Hit

[FAIL]Adapter Content

Adapter graph