FastQCFastQC Report
Sat 31 Dec 2022
SRR5849736.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849736.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116902
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC23021.9691707584130296No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT22081.8887615267489006No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA5100.4362628526458059No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA3750.32078150929838667No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC2570.219842261039161No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT2220.1899026535046449No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC1600.136866777300645No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1510.1291680210774837No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC1500.12831260371935468No Hit
TATAGCGGGGTTTTATATAATAGTGAAGTTGTCATGAAAATCATTTAGAGTG1440.12318009957058049No Hit
AGCGAAGGGGGGAACTATCAGCATAGAAATGGAAATTAAAACACACTACTAA1330.11377050863116114No Hit
ACTGCTGGGATTACAGGTATGAATTCTTTCTAATAAGTTAACCAGGCTTCAA1300.11120425655677406No Hit
TGTGCATGGGACAAGCAACTGCTGAATGAATGGTAGTTATAGCCTCCTCCTC1280.109493421840516No Hit
GTCACTGGGATGAAATGAGAGGATGCTTTTGAAAACACTTTAACATGATGTT1260.10778258712425794No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGT1250.10692716976612889No Hit
ACCAAAGGGATATGAGCTGAGTTATTATGCCTGTGTCAAAATTACGTGGGCT1250.10692716976612889No Hit
TTCAGGGGGGAGGCGCCTGGAAGAATTGCTTCGACAAATGTGTCAAGCAGCA1200.10265008297548374No Hit
CACATAGGGGAAGGAATTGTAAGTTTCAGTTGCTGAATATATCAGTATATCT1190.10179466561735472No Hit
GTTATCGAGGGGAGCCAGGGACCTTTTAACAACCAGCTGTCATGTGAACTCA1190.10179466561735472No Hit
CATTGGACATTTTTCTTGCATTGCCTCAGTAACAGAATAGAGGAGATATATT1180.10093924825922568No Hit
GTGTCCGGGGAGATGATGCTGGTCTGGGCGAGAATGTCAGGAATGGAGGTGA1180.10093924825922568No Hit
AGAGTAGGAAACAAACTTTTGTAAATAGACCTTACCTTCTATTTTCGAGTTT1170.10008383090109665No Hit
CCAACTCCAGATTCCTGTCACGCGCCTTCCAGTGCAAGAAATGATCATGCTT1170.10008383090109665No Hit
GTAATAGGGACAAACAAGAGATGGTAAATCAGTGTACTGTGTTTAAGTGGTA1170.10008383090109665No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)