FastQCFastQC Report
Sat 31 Dec 2022
SRR5849735.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849735.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117123
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT22311.9048350878990463No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC21111.8023786959008903No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA5100.4354396659921621No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA4100.35005933932703226No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC2950.251871963662133No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC2150.18356770233002911No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT1800.15368458799723367No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCC1720.14685416186402328No Hit
AGCGAAGGGGGGAACTATCAGCATAGAAATGGAAATTAAAACACACTACTAA1410.12038626059783304No Hit
ACCAAAGGGATATGAGCTGAGTTATTATGCCTGTGTCAAAATTACGTGGGCT1400.11953245733118176No Hit
TGTATAGGGAACGTTCTTGTGAATTTAGAATGTGTGAATAGCTCTCTAAGAT1300.11099442466466877No Hit
CATTGGACATTTTTCTTGCATTGCCTCAGTAACAGAATAGAGGAGATATATT1250.10672540833141228No Hit
GTTATCGAGGGGAGCCAGGGACCTTTTAACAACCAGCTGTCATGTGAACTCA1240.10587160506476097No Hit
TGTTTCGGGATTAAGGGTATGTTAGGACTGTTTGGAAGAGGTAACGTAAATA1230.10501780179810968No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAT1230.10501780179810968No Hit
GTCACTGGGATGAAATGAGAGGATGCTTTTGAAAACACTTTAACATGATGTT1220.10416399853145838No Hit
TGTGCATGGGACAAGCAACTGCTGAATGAATGGTAGTTATAGCCTCCTCCTC1220.10416399853145838No Hit
GCTACCGGGACCACATCTGAAGCCTGGAATTTCCAAGTTCCTGTATGCAGTG1210.10331019526480709No Hit
CACATAGGGGAAGGAATTGTAAGTTTCAGTTGCTGAATATATCAGTATATCT1180.10074878546485319No Hit
TGCCATGGGATTGCCAAATTCAGTAGTTGTACATTTTGCATTATTTGCTTTA1180.10074878546485319No Hit
CAGACAGGGAGAAAATGGAAGAAACAAAAGATAGACTCACTGAAAAGTTACA1180.10074878546485319No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)