FastQCFastQC Report
Sat 31 Dec 2022
SRR5849733.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849733.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231217
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC27201.176384089405191No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT25621.1080500136235658No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA5710.24695415994498673No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA4080.17645761341077862No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC2900.12542330364981813No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCT2750.11893589139206893No Hit
GAGTAAGGGCTATTTCATCAAGTTTGATGATGATTTCCATATTTATGTCTAC2330.10077113707037114No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)