FastQCFastQC Report
Sat 31 Dec 2022
SRR5849732.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849732.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229002
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGC26531.1585051658937477No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCTCGTATGCCGTCT25371.1078505864577601No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCA5470.2388625426852167No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAA3940.172050899118785No Hit
CTTATACACATCTCCGAGCCCACGAGACTATGCTCAATCCTGTCTCTTATAC3010.13143990008820883No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTATGCTCAAACTCGTATGC2780.12139631968279753No Hit
TGCACCTGGGATTTCAGAATAAACTATAATTTATATAAGCTGAGTAATAATT2410.10523925555235326No Hit
TGTCTAGGGAGAGTGTTTCAATTTCGACCCATCAGTTGTGAACCACTAATTA2390.10436590073449141No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)