FastQCFastQC Report
Sat 31 Dec 2022
SRR5849728.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849728.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52515
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT3820.7274112158430924No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC3160.6017328382366942No Hit
CTCTCTATGGGGAGCTGGGAGCCAGGCTCTCCGGGCCTTTCTCTGGCTTCCT860.16376273445682185No Hit
TCCTTTGGGAGGGATCCCTGAGGATGATTGAGACCAAATTTCCCACTACCCC830.15805008092925832No Hit
TATGCCGGGACAATTCAAACAAGACTTGGTCAGCGCAGAACTGCAAGGCTGT760.14472055603161002No Hit
GATGCCGGGGCGACTTATGGTAGCCAAGGAAGCCCGCAATGTTACCATGGAA750.14281633818908884No Hit
AGTTAAGGGATAGCTATTGCTGTATTGTGCTCAGGATTTGCAGGAGTATATT730.13900790250404646No Hit
AAGGCTGGGATAACTCGCTGCTCCTGAAGCACCAGCGCATCCACACGGGCGA730.13900790250404646No Hit
TTAAGTGGGATCTGGAGTGCAATGGGCAGTGGAAGAGGATTGGGGATCCCAG710.13519946681900408No Hit
GTAATCGGGGACACAGAGTCTCGCCATGTTGCCCAGGCTGGTCTTGAACTCC710.13519946681900408No Hit
CAAGGTGGGGGGGGTGCTCAGGGGTGCGTGTGTGTACGTGCGTGTTCTGTGT700.1332952489764829No Hit
CGTTCGAGGGATATTATTGTGTCTTTGTCAAACAACAAAAACTTGAACTTAG690.13139103113396172No Hit
TCGATGGGGACTGATATTGCCAGTTTCTGCTATCTCCCTTTTTTCTTTAGGT670.12758259544891937No Hit
GTACGCGGGAACTTTATAAACTTTTTCAGTGTAATTTTATTCCATCTCAGAG670.12758259544891937No Hit
TGACATGGGGGATCACACACGAGCACACACCCAAACACGTGCATATCACACA660.1256783776063982No Hit
CGTAATGGGGTCTGGAATTGCAACTAGTCCTGCCTCCGGTCCTCCATGATGA650.123774159763877No Hit
TTTACGGGGAATTTTAAAATGGTTCTTAAGTTTTCTGTGGGTATTGAAACTG640.1218699419213558No Hit
TGACGAGGGGGATTTCAAAAAGGACTTGAAGATGTGAATCATCTATTTTGCT640.1218699419213558No Hit
CAGTTGGGGGGCTCCGGAGCAACCCGCGAGCTTACACCGGCTTCTCTCTGTC640.1218699419213558No Hit
CGAATTGGGACACATTAATAATGGGAGACTTTAACACCCCACTGTCAACATT640.1218699419213558No Hit
CAGCGGGGAGGCGGGGATGCTGCGGCTCCGCAGCGGGCTGAGGCACCTTCGG640.1218699419213558No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCCTGTCTCTTATAC640.1218699419213558No Hit
TAATGGGGGATTTTGCGAACGGCGAGCAGCGGCGGCGGCGCGGAGAGACGCA630.11996572407883462No Hit
GCAAATGGGAGGAAAGCAGGGCTTAGCAAGGGCTATCTAGGGCCTTGACCAA630.11996572407883462No Hit
TCCGCGGGATTGTTTAAAGTGATTACATTTAACGTCCTCTCCCATGACTTCA630.11996572407883462No Hit
AAGCTGGGGCATCTACTCTTTGAAGCTTCTGCCCAGCTTGCATTGTTTCTAG620.11806150623631342No Hit
GCGCATGGGGACTGCCAGGGCTGTGCCTACAAAAAAGGCATCTGTGCGATGT620.11806150623631342No Hit
TCAGTTAGGGAAGCTTATTGTGTTTAAGAAAGTAGCTATGTGTTAAACAGAG620.11806150623631342No Hit
TCTACAGGGACTTTATCATGGTGGATAAGCTTTTTGATGTGTTGCTGGATTT610.11615728839379225No Hit
ACTGCTGGGAAGTATTAAATACAGATTGTGTATGCATTCTTTGTGTGTATAT610.11615728839379225No Hit
GCTCCCGGACACAAACATACTGTCTTTTTATGTTCACAGCTCTAGAAATTTA610.11615728839379225No Hit
GCAACTGGGAATACTCCATTGCATGTAAATACCACATTTTGTTTATACATTC610.11615728839379225No Hit
TCATAGGGGAAATTAAAATCAGCAGATTGAGTTTTCAACATTGCAAAATCAG600.11425307055127107No Hit
TTCGGCAGGGTGCACAGACACCTTGTGAGTGATTGGTATTGGAGGTGTTCAA600.11425307055127107No Hit
TTTGAGGGTATTGCAATTGGACTGGAGGCTATAAGTGGTCTACGGCTCTGGC600.11425307055127107No Hit
CGAAGCGGGAGTGCCTAGAGGGAAATTAATAGTATTAAAAGCTTGTTAGAGG600.11425307055127107No Hit
GTAGAGGGGATTGTTTTTAAAGATGTACATATCTTTTAAAATCTTTATCTCT590.11234885270874988No Hit
AAGCAAGGGTAAGGTCTTTTCCTCATAATTTTCTAAATACGATAAAGTGATA590.11234885270874988No Hit
CTTTATGGGGAGAATAACCTTGAAGAAGACTCCCTTGCAATGAGCAACAACA590.11234885270874988No Hit
TCGACAGGGAGTCTCTTCTCAAATCAATCAGTACCTGCTGACACTGTTCAGT580.1104446348662287No Hit
GATACTGGGACTTGTACCAACAAAACTTAATTTTTGACACACCTCTAATAAC570.10854041702370752No Hit
TGTAGCGGGGCTTCCTCTTTCCCTAAGCAGCCTGAGGTGATCTGTGAAAATG570.10854041702370752No Hit
GAACGGGGATCTATCTACGGGGTCTTTGGGAGATTTAAATTGCGCTAACAGT570.10854041702370752No Hit
ACTTTCGGGAACACTGGAGCTCCCAATTTTACAAAGCAATTACTAATAGACC560.10663619918118633No Hit
CTTTGTGGGTGGCCACATGGCCATCGCTGCCCCTTGTTGACACCTCCTTTCT560.10663619918118633No Hit
CTAATGGGGATATTGTTAAAAAATTAAAACACTTCTAATAAACAAAATCCCT560.10663619918118633No Hit
ATCAGCGGGGAACTCTGGAAACCAACCAAAGATAGTTAGGGAAGAAAGAAAC560.10663619918118633No Hit
TGGCTTGGGAGGGCTGCGACAGCTTCAACGCCCAGGTCATCGTCCTCAACCA560.10663619918118633No Hit
ATGGCGGGACCATAGAAAGAGAAATTATTCAGTCAAAACTGTGGGTAGCATA560.10663619918118633No Hit
AACCGGGGAATTTATCTGAGGCAGCTACAGATTGCCCTGCAAGATGAGTTTT560.10663619918118633No Hit
ATTCATGGGACTAGCCTTGGGATAGGGAATATTAAAACAATTGCAGCTCCAC560.10663619918118633No Hit
TTAACGGGGATCATTTTTGCAGTGTATGTTGGAGCTGTAATGGTTGCAATTA550.10473198133866514No Hit
ACAGCCGGGGCTTTTCTGTGGGGGTGGGATTTGGAAGGGGGGTTGGGTTGGG550.10473198133866514No Hit
AGCCTTGGGCAATGTGGAATTACATAACAGGCATTGCACTAAAAGTGATGTG550.10473198133866514No Hit
TCGAACGGGGTGGCAAATAAATGTTTGACTAAAATTTCTGGCTGTCTTCATA550.10473198133866514No Hit
TGAAGCGGGAGATAAAATTAAACCCAGTGAATCTAATGTGACTATTCTTGGG550.10473198133866514No Hit
ACATCAGGGATTCCTGTTCCTACTACAGTTCCTGTTCCTGTGCCAGTTCCTG550.10473198133866514No Hit
CTGCTTGGGCAGTTGTACAGCCACTGTACAGCTAACATCTCAACACTGGAAA550.10473198133866514No Hit
TATGCAGGGACCATTTGCCTTATAAAAATGGCCCACAACCTTCACAATATTT540.10282776349614395No Hit
ATTGGTGGGTACAGTGAGAAGGTACTTAATGCCCCTGAACTGTATACTTAAA540.10282776349614395No Hit
TATCAAGGGGTTTACTAGGATACATATACAGGAGTATTTGTGACAGCACTGT540.10282776349614395No Hit
CCATGGGCGATCTTGCGTGGGCTTCCAACAGAGATGATGCTGTTCGTATGTT540.10282776349614395No Hit
TGCTGTGGGAATAGCTGGCCTTGCTAGAATCCTTGGTTTCACTGCTGTTCTT540.10282776349614395No Hit
ACTCCGGGGGTTTATTTTTGGCTAGAGGCTTACCACCTTTTACATTTTATTA540.10282776349614395No Hit
GGTCCTGGGGGAAACATTCATTTGTTAAAATGCTAAAAAGTAAAATATTTAT540.10282776349614395No Hit
AAAAATGGGATTCAAGCCAATAGCTGGATTTTTGAAATGACTAATTGATGAG530.10092354565362277No Hit
TCTTAAGGGATGACTGGCTCCAAGTTTGACACACAACATTTCCAGTGGTGGT530.10092354565362277No Hit
GGACCAGGGCGATCTTGCTCCGGGAAGAATTTAGTGCCAGGGACCACTTTTT530.10092354565362277No Hit
ATTTGCGGGCAGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCGG530.10092354565362277No Hit
GCTTTAGGGACTGGGCTTTGCGCCGGGAAAAATCTGAATACTGGAGCTACAG530.10092354565362277No Hit
GGTAGTGGGAAGACCCGAAAGCAGAAGTAATACTGGGAACCCTTTCTGGGTC530.10092354565362277No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)