FastQCFastQC Report
Sat 31 Dec 2022
SRR5849727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51829
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT3300.6367091782592758No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC3090.5961913214609581No Hit
AAGGCTGGGATAACTCGCTGCTCCTGAAGCACCAGCGCATCCACACGGGCGA900.1736479577070752No Hit
TAATCCGGGGGAGACATTGTCATTTCAAATGCATATGTGGATCTTGCACCAA880.16978911420247353No Hit
CGTTCGAGGGATATTATTGTGTCTTTGTCAAACAACAAAAACTTGAACTTAG790.15242431843176601No Hit
TGACATGGGGGATCACACACGAGCACACACCCAAACACGTGCATATCACACA770.14856547492716432No Hit
TCCGCGGGATTGTTTAAAGTGATTACATTTAACGTCCTCTCCCATGACTTCA760.1466360531748635No Hit
GCATACGGGATTGTGAAAAGCTTTTAAAAGTTGTTTTGAAAAGACAATCATT720.13891836616566014No Hit
GATGCCGGGGCGACTTATGGTAGCCAAGGAAGCCCGCAATGTTACCATGGAA720.13891836616566014No Hit
TATGCAGGGACCATTTGCCTTATAAAAATGGCCCACAACCTTCACAATATTT690.13313010090875765No Hit
TATCAAGGGGTTTACTAGGATACATATACAGGAGTATTTGTGACAGCACTGT690.13313010090875765No Hit
ATGGCGGGACCATAGAAAGAGAAATTATTCAGTCAAAACTGTGGGTAGCATA680.1312006791564568No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATG680.1312006791564568No Hit
TCGAACGGGGTGGCAAATAAATGTTTGACTAAAATTTCTGGCTGTCTTCATA670.129271257404156No Hit
TGACGAGGGGGATTTCAAAAAGGACTTGAAGATGTGAATCATCTATTTTGCT670.129271257404156No Hit
CCATGGGCGATCTTGCGTGGGCTTCCAACAGAGATGATGCTGTTCGTATGTT660.12734183565185514No Hit
ATTTGCGGGCAGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCGG660.12734183565185514No Hit
TCCTTTGGGAGGGATCCCTGAGGATGATTGAGACCAAATTTCCCACTACCCC650.12541241389955432No Hit
TGCCAGGGGATCCCCATCCTGTTTCAAATTTCTGAAAAATGTCAACAGCAAT650.12541241389955432No Hit
TCGATGGGGACTGATATTGCCAGTTTCTGCTATCTCCCTTTTTTCTTTAGGT650.12541241389955432No Hit
GCTCCCGGACACAAACATACTGTCTTTTTATGTTCACAGCTCTAGAAATTTA650.12541241389955432No Hit
ACTTTCGGGAACACTGGAGCTCCCAATTTTACAAAGCAATTACTAATAGACC640.12348299214725347No Hit
GCAGTTGGGGGTTGGACCTGAGTCTAGACTGACCCTAGAGTTGGAAATTTAG640.12348299214725347No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCCTGTCTCTTATAC640.12348299214725347No Hit
CGAAGCGGGAGTGCCTAGAGGGAAATTAATAGTATTAAAAGCTTGTTAGAGG640.12348299214725347No Hit
TAGCGCGGGATCCTTGCCAATATTTGGATTTGTCAGTGTTTTGGAGATCGGC630.12155357039495263No Hit
TCTCTTGGGGAAAAGAGCCAATCTAAAAAAGCTACCTATCATACGATTCTGT630.12155357039495263No Hit
CTGCTTGGGCAGTTGTACAGCCACTGTACAGCTAACATCTCAACACTGGAAA630.12155357039495263No Hit
CAAGGTGGGGGGGGTGCTCAGGGGTGCGTGTGTGTACGTGCGTGTTCTGTGT620.1196241486426518No Hit
GCAAATGGGAGGAAAGCAGGGCTTAGCAAGGGCTATCTAGGGCCTTGACCAA620.1196241486426518No Hit
TCAGTTAGGGAAGCTTATTGTGTTTAAGAAAGTAGCTATGTGTTAAACAGAG620.1196241486426518No Hit
GTTTTAGGGGTCAAATTAAACTGATTAAAATTTTTGGAACTTTTTAGCATTC620.1196241486426518No Hit
TCTACAGGGACTTTATCATGGTGGATAAGCTTTTTGATGTGTTGCTGGATTT610.11769472689035097No Hit
AAAAATGGGATTCAAGCCAATAGCTGGATTTTTGAAATGACTAATTGATGAG610.11769472689035097No Hit
GAGATGGGACGGCAGGCGTCCGCGTCGCTAGCTAGTCGTTCTGAAGCGGCGG610.11769472689035097No Hit
TTCTAGGGAGACTCCCAATTCTCTTCTTTTCCAGGATGTACACTAGAATCTG610.11769472689035097No Hit
AACCGGGGAATTTATCTGAGGCAGCTACAGATTGCCCTGCAAGATGAGTTTT610.11769472689035097No Hit
CGTAATGGGGTCTGGAATTGCAACTAGTCCTGCCTCCGGTCCTCCATGATGA600.11576530513805013No Hit
CTAATGGGGATATTGTTAAAAAATTAAAACACTTCTAATAAACAAAATCCCT600.11576530513805013No Hit
TTTACGGGGAATTTTAAAATGGTTCTTAAGTTTTCTGTGGGTATTGAAACTG600.11576530513805013No Hit
CAGCGGGGAGGCGGGGATGCTGCGGCTCCGCAGCGGGCTGAGGCACCTTCGG600.11576530513805013No Hit
CTCTCTATGGGGAGCTGGGAGCCAGGCTCTCCGGGCCTTTCTCTGGCTTCCT600.11576530513805013No Hit
TGGTCAGGGATTATTGTCTCTTTATTTGAGTGTCTCGACAACATGTCTCTCT590.11383588338574929No Hit
TAATGGGGGATTTTGCGAACGGCGAGCAGCGGCGGCGGCGCGGAGAGACGCA590.11383588338574929No Hit
CCAACTGGGAGGAATGCCTACTGCAGGGCTAACACTGGCAATATGGCAGCTT590.11383588338574929No Hit
TAGAATGGGAGTGAAAAAAATTACAAAAGAACAAAGTAATTATTCACAAGAT590.11383588338574929No Hit
ATTCATGGGACTAGCCTTGGGATAGGGAATATTAAAACAATTGCAGCTCCAC590.11383588338574929No Hit
CATATAGGGAACACACTTGTAACCCATTTGTGCACACAGGTGTGCCGTGACT580.11190646163344847No Hit
AAATTAGGGATCTGACAAGCGGGACTCTTTAATACAATAGTTCACGTTCCTG580.11190646163344847No Hit
TGTAGTGGGAAGTTAAATCAACTGAAGGACATAGTTTCTGGCTAGGGCAGGG580.11190646163344847No Hit
TGTTGCGGGGTCCCAGTTCTCTCCTACAGAAAAAATACTTTCAGTATGTTTT580.11190646163344847No Hit
CGAATTGGGACACATTAATAATGGGAGACTTTAACACCCCACTGTCAACATT580.11190646163344847No Hit
GCAACTGGGAATACTCCATTGCATGTAAATACCACATTTTGTTTATACATTC580.11190646163344847No Hit
ATTGGTGGGTACAGTGAGAAGGTACTTAATGCCCCTGAACTGTATACTTAAA570.10997703988114761No Hit
TAACCACGGGATTCTTTTATTGTCAGTATCTGTATCTCTCTCTCTTTTTGGA570.10997703988114761No Hit
ATCCGTGGGAACTAGGTGTCCAAATTATCGAAGTGTGCCCGGCGCTGAAGCA560.10804761812884679No Hit
CTTTGTGGGTGGCCACATGGCCATCGCTGCCCCTTGTTGACACCTCCTTTCT560.10804761812884679No Hit
TGAAGCGGGAGATAAAATTAAACCCAGTGAATCTAATGTGACTATTCTTGGG560.10804761812884679No Hit
TGTGTTGGGAGACATTCCAGGGATGGGAGGGGCCAGGGATCATGTCTTACTC560.10804761812884679No Hit
AATGCCGGGACTCCATGACCTGTTAAAACCAGCAATGTGAGTATTATTTGGA560.10804761812884679No Hit
TTAAAGGGGAGGAGGCAGGTAGATAACTCCAGCTCTTGACTAGTCTGTGGTT550.10611819637654596No Hit
TTCAGAGGATACTTAAAATCCTAGGAAGTTATCTGTAACCAGTCTCTTGTCT550.10611819637654596No Hit
GCAATCGAGGGAAATGACATAATGAATGAAATCATGAAACGGAATAATGAAA550.10611819637654596No Hit
CACACTGGGACTTTGGGGTTTGTAGATTGAACCTTTCAAGGCAATTTTCTCA550.10611819637654596No Hit
TTGCCTGGGACTGTTTTGAAAATCTGAAAAACTAAAACACACTATGGGTGTG550.10611819637654596No Hit
TAGATAGAGACACAAAAAAAGCCTTCAAAAAATCAATGAATCCAGGAGCTGG550.10611819637654596No Hit
TGGACAAGGGATCAATTAAAATGTATTTGTAAGTTTCATCGGCCTTGATTGT550.10611819637654596No Hit
TCACTAGGGAGGGACCAATCCTGGAGAGATAGAGATTTGCGGCCTTTCAGAC540.10418877462424511No Hit
CGCACGGGGAAAGTGAGGAAGTACCAAAGAGTTGTGTGGCTGGGTATTAGCC540.10418877462424511No Hit
GCGTTGGGGAGTAGAGGCCAGGGATCCTGCTAAATAACCTGCATTGGACAGC540.10418877462424511No Hit
GTACGCGGGAACTTTATAAACTTTTTCAGTGTAATTTTATTCCATCTCAGAG540.10418877462424511No Hit
CGCTCGGGGAACAGAACATTCCTTTAGGTACAGAAAGAAAAAAGGGGAAAGA530.10225935287194428No Hit
AGCAATGGGAGAAATCTATATATTAGACCTTAAAGAGAATGACTATGTTCTG530.10225935287194428No Hit
TGTAGCGGGGCTTCCTCTTTCCCTAAGCAGCCTGAGGTGATCTGTGAAAATG530.10225935287194428No Hit
CAAAGCGGGCTGTTGCCTAGAGACTGAGAACCAGCCCACATGTGACCTGTCA530.10225935287194428No Hit
AGATCGGGTTTGTATAAGAAATCATTGCTTAGTTTGAGGTTTTGAAAGTGTC530.10225935287194428No Hit
CCAAATGGGGATGTTTCTGCAGGGGAATGAGCACTGGAAAGTGCTATTTTGC520.10032993111964343No Hit
GCGCATGGGGACTGCCAGGGCTGTGCCTACAAAAAAGGCATCTGTGCGATGT520.10032993111964343No Hit
GTAGAGGGGATTGTTTTTAAAGATGTACATATCTTTTAAAATCTTTATCTCT520.10032993111964343No Hit
ATCAGCGGGGAACTCTGGAAACCAACCAAAGATAGTTAGGGAAGAAAGAAAC520.10032993111964343No Hit
AAAAAGGGGAAGTAAATTTGCTGCCCTCCACAGCCTTCTAATTTTATTTATA520.10032993111964343No Hit
TTCGGCAGGGTGCACAGACACCTTGTGAGTGATTGGTATTGGAGGTGTTCAA520.10032993111964343No Hit
ACTCCGGGGGTTTATTTTTGGCTAGAGGCTTACCACCTTTTACATTTTATTA520.10032993111964343No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)