FastQCFastQC Report
Sat 31 Dec 2022
SRR5849726.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849726.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52382
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT3340.6376236111641403No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC2540.48489939292123246No Hit
GCAACTGGGAATACTCCATTGCATGTAAATACCACATTTTGTTTATACATTC780.1489061127868352No Hit
TTAAGTGGGATCTGGAGTGCAATGGGCAGTGGAAGAGGATTGGGGATCCCAG730.13936084914665342No Hit
ATGGCGGGACCATAGAAAGAGAAATTATTCAGTCAAAACTGTGGGTAGCATA730.13936084914665342No Hit
CGTTCGAGGGATATTATTGTGTCTTTGTCAAACAACAAAAACTTGAACTTAG720.13745179641861707No Hit
GCGCATGGGGACTGCCAGGGCTGTGCCTACAAAAAAGGCATCTGTGCGATGT720.13745179641861707No Hit
TCCTTTGGGAGGGATCCCTGAGGATGATTGAGACCAAATTTCCCACTACCCC720.13745179641861707No Hit
TGACGAGGGGGATTTCAAAAAGGACTTGAAGATGTGAATCATCTATTTTGCT720.13745179641861707No Hit
CGAAGCGGGAGTGCCTAGAGGGAAATTAATAGTATTAAAAGCTTGTTAGAGG710.13554274369058072No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATG700.13363369096254438No Hit
CTCGTTGGGAAGAAAGGATATAGTTTTACGTAACATTTGGGGAAAAACAGTT690.13172463823450803No Hit
GCTCCCGGACACAAACATACTGTCTTTTTATGTTCACAGCTCTAGAAATTTA690.13172463823450803No Hit
TGGTCAGGGATTATTGTCTCTTTATTTGAGTGTCTCGACAACATGTCTCTCT670.12790653277843536No Hit
TTCGGCAGGGTGCACAGACACCTTGTGAGTGATTGGTATTGGAGGTGTTCAA670.12790653277843536No Hit
GAACGGGGATCTATCTACGGGGTCTTTGGGAGATTTAAATTGCGCTAACAGT670.12790653277843536No Hit
ATTTGCGGGCAGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCGG670.12790653277843536No Hit
TTAACGGGGATCATTTTTGCAGTGTATGTTGGAGCTGTAATGGTTGCAATTA660.125997480050399No Hit
TGAAGCGGGAGATAAAATTAAACCCAGTGAATCTAATGTGACTATTCTTGGG660.125997480050399No Hit
TATGCAGGGACCATTTGCCTTATAAAAATGGCCCACAACCTTCACAATATTT640.1221793745943263No Hit
TAGCGCGGGATCCTTGCCAATATTTGGATTTGTCAGTGTTTTGGAGATCGGC640.1221793745943263No Hit
TCTCTTGGGGAAAAGAGCCAATCTAAAAAAGCTACCTATCATACGATTCTGT640.1221793745943263No Hit
CGAATTGGGACACATTAATAATGGGAGACTTTAACACCCCACTGTCAACATT630.12027032186628994No Hit
AGTTAAGGGATAGCTATTGCTGTATTGTGCTCAGGATTTGCAGGAGTATATT620.1183612691382536No Hit
CAAGGTGGGGGGGGTGCTCAGGGGTGCGTGTGTGTACGTGCGTGTTCTGTGT620.1183612691382536No Hit
TCAAACGGGGTCTACCAACTGTGAACTACCAACTGACTTCAGTCTATTACAA620.1183612691382536No Hit
GATGCCGGGGCGACTTATGGTAGCCAAGGAAGCCCGCAATGTTACCATGGAA620.1183612691382536No Hit
GTCCGCGGGAATGACCGGTGCCTGTAATCCCAGCCACTCGGGAGTCTGAGGC620.1183612691382536No Hit
TGTCCTGGGGGCGCCATCAGAGCTGGAGCACCTTCAAATCTATGACACTGGG610.11645221641021725No Hit
TAGAATGGGAGTGAAAAAAATTACAAAAGAACAAAGTAATTATTCACAAGAT610.11645221641021725No Hit
AAGGCTGGGATAACTCGCTGCTCCTGAAGCACCAGCGCATCCACACGGGCGA610.11645221641021725No Hit
TGACATGGGGGATCACACACGAGCACACACCCAAACACGTGCATATCACACA610.11645221641021725No Hit
ATCAGCGGGGAACTCTGGAAACCAACCAAAGATAGTTAGGGAAGAAAGAAAC600.1145431636821809No Hit
TTTACGGGGAATTTTAAAATGGTTCTTAAGTTTTCTGTGGGTATTGAAACTG600.1145431636821809No Hit
CAGCGGGGAGGCGGGGATGCTGCGGCTCCGCAGCGGGCTGAGGCACCTTCGG600.1145431636821809No Hit
GCATACGGGATTGTGAAAAGCTTTTAAAAGTTGTTTTGAAAAGACAATCATT600.1145431636821809No Hit
AAATTAGGGATCTGACAAGCGGGACTCTTTAATACAATAGTTCACGTTCCTG590.11263411095414455No Hit
TGGCTTGGGAGGGCTGCGACAGCTTCAACGCCCAGGTCATCGTCCTCAACCA590.11263411095414455No Hit
TATCAAGGGGTTTACTAGGATACATATACAGGAGTATTTGTGACAGCACTGT590.11263411095414455No Hit
TGCTGTGGGAATAGCTGGCCTTGCTAGAATCCTTGGTTTCACTGCTGTTCTT590.11263411095414455No Hit
TCCGCGGGATTGTTTAAAGTGATTACATTTAACGTCCTCTCCCATGACTTCA590.11263411095414455No Hit
GCGCTGGGACTGTTGTTTCACTTTCTGGATAGTGTCCTCTGAAGCACAAAAG580.1107250582261082No Hit
AACCAGGGGGGATTTCTAGGTGGAGTAATATTTTCCTGTTCAAATTATTTCC580.1107250582261082No Hit
TCAGTTAGGGAAGCTTATTGTGTTTAAGAAAGTAGCTATGTGTTAAACAGAG580.1107250582261082No Hit
CTTTGTGGGTGGCCACATGGCCATCGCTGCCCCTTGTTGACACCTCCTTTCT570.10881600549807187No Hit
ATTGGTGGGTACAGTGAGAAGGTACTTAATGCCCCTGAACTGTATACTTAAA570.10881600549807187No Hit
AAAAAGGGGAAGTAAATTTGCTGCCCTCCACAGCCTTCTAATTTTATTTATA570.10881600549807187No Hit
TGTAGTGGGAAGTTAAATCAACTGAAGGACATAGTTTCTGGCTAGGGCAGGG570.10881600549807187No Hit
TATGCCGGGACAATTCAAACAAGACTTGGTCAGCGCAGAACTGCAAGGCTGT570.10881600549807187No Hit
TTGCCTGGGACTGTTTTGAAAATCTGAAAAACTAAAACACACTATGGGTGTG570.10881600549807187No Hit
TGTAGCGGGATTCCTCGGGGCTTCAGAGTTAGTGAGGTGGCTTCTACAGTGT570.10881600549807187No Hit
ACATCAGGGATTCCTGTTCCTACTACAGTTCCTGTTCCTGTGCCAGTTCCTG570.10881600549807187No Hit
AAGCTGGGGCATCTACTCTTTGAAGCTTCTGCCCAGCTTGCATTGTTTCTAG560.10690695277003552No Hit
AAGTAGGGGGGTTTTGCCATTAAAAGTAATGGCAAAGGAAATAACCTTTGCA560.10690695277003552No Hit
TACGCTGGGGATATGTGCTAGGCAAATCTCCAACACAGTTGATGTGTAGTCT560.10690695277003552No Hit
GCAGTTGGGGGTTGGACCTGAGTCTAGACTGACCCTAGAGTTGGAAATTTAG560.10690695277003552No Hit
GTTTTAGGGGTCAAATTAAACTGATTAAAATTTTTGGAACTTTTTAGCATTC560.10690695277003552No Hit
GTACGCGGGAACTTTATAAACTTTTTCAGTGTAATTTTATTCCATCTCAGAG560.10690695277003552No Hit
CCAAATGGGGATGTTTCTGCAGGGGAATGAGCACTGGAAAGTGCTATTTTGC550.10499790004199916No Hit
ATCCGTGGGAACTAGGTGTCCAAATTATCGAAGTGTGCCCGGCGCTGAAGCA550.10499790004199916No Hit
GGCATAGGGGTATTCTTCTCTTCATCAACCTTTTGTGGTGATGGGTTGCCTT550.10499790004199916No Hit
TTTAGAGGGATGAGAAATTGAGTTCTTTTAGTAAAATAAATAAGACTGAGAT550.10499790004199916No Hit
TGTGTGGGGGACTTCTGTTAATCATCAGAATATTTATTTAATGTGTTTAATG550.10499790004199916No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCCTGTCTCTTATAC550.10499790004199916No Hit
ACTCCGGGGGTTTATTTTTGGCTAGAGGCTTACCACCTTTTACATTTTATTA550.10499790004199916No Hit
GGTCCTGGGGGAAACATTCATTTGTTAAAATGCTAAAAAGTAAAATATTTAT550.10499790004199916No Hit
TGTAGCGGGGCTTCCTCTTTCCCTAAGCAGCCTGAGGTGATCTGTGAAAATG540.10308884731396281No Hit
TCTACAGGGACTTTATCATGGTGGATAAGCTTTTTGATGTGTTGCTGGATTT530.10117979458592646No Hit
ACACAAGGGATTTAAAAATTGGTAATACATGTAATTCAGAAATCAGGAATTT530.10117979458592646No Hit
GCAAATGGGAGGAAAGCAGGGCTTAGCAAGGGCTATCTAGGGCCTTGACCAA530.10117979458592646No Hit
CACACTGGGACTTTGGGGTTTGTAGATTGAACCTTTCAAGGCAATTTTCTCA530.10117979458592646No Hit
TCGATGGGGACTGATATTGCCAGTTTCTGCTATCTCCCTTTTTTCTTTAGGT530.10117979458592646No Hit
GAAGGCGGTCCTGAGCTCATGATTTAGTGTGTTGTTAAGCGTAATTCTGTTG530.10117979458592646No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)