FastQCFastQC Report
Sat 31 Dec 2022
SRR5849725.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849725.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62337
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT6921.1100951280940694No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC5590.8967386945152959No Hit
GCTGATGGGCAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT1630.26148194491233134No Hit
CAAGTGGGGAGTCTGAAACAGCAGCTTCTGAGGAAACCTTGTTCAACGCGTC1420.2277940869788408No Hit
GCTCGGGATGGTGCAGACATGGCCAAGTCCAAGAACCACACCACACACAACC1350.21656480100101066No Hit
GCAATAGGGAAGTTTCTCAGAGAAACAGTGTATGTGATGGCAGGACCCCAGT1300.20854388244541766No Hit
CTCTAAGGGAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCT1280.20533551502318045No Hit
GTGGTGGGACCTATAAATGGACTTGCTTCTTGTGTTTGAAACACTCGTGTGC1220.19571041275646886No Hit
GCTGCTGGGAGGACTCACTGCCCTCCAGAAAACAGAGGTGACAAATGTGAGG1200.19250204533423168No Hit
AGTCAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1170.18768949420087588No Hit
TGCTAGGGGTCGGTATGTTCAAACTCAGACACGAAAACCAGAACTGCATCAG1150.18448112677863868No Hit
AAGCTAGGGTTATAATAAGATTAAGAGACATAATAATAAAATGCAATGTATT1110.1780643919341643No Hit
CTACGCGGGATTGTCCTGGGTGGACCAATCCAAAGGCTAAGTGATAGTGACT1090.1748560245119271No Hit
AATACGGGGAAAGAAGGGGACATCATTACATTAACCAATCAAATAGATGAAA1080.17325184080080852No Hit
CTTATGGGGATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAG1030.16523092224521552No Hit
GCTTCAGGGCAAGACTAATAAGATGCCTAAAAACCCTTAAACCCTAATTCAT1000.16041837111185972No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATG950.15239745255626674No Hit
TCAGAAGGGAGTGTGGCCCGGGCGTCCGCTCCTCAGCGGATGTGGCAGCCCC950.15239745255626674No Hit
CAGCCTGGGTGACAGAGTGGGATACCATCTCTTCAGAAAAAAACAAAGATTT950.15239745255626674No Hit
ACGAGGGAAGGGATTCTCCTGCCTCAGCTTCCTAAGTAGCTGGGATTACAGG920.14758490142291095No Hit
CAATGAGGGACTCTTATAGCCTGCCTTCAATTTTGATCCTTTATTCTTTCTA900.14437653400067377No Hit
TTGTTCAGGGCTATGGAAACTCATCTGATTCCAGATATGATGATGGAAGAGG890.14277235028955518No Hit
CATATTGGGGCTCGCGGCGCTCGAGGGACCATGGCCGATCCTCGCGTGAGAC860.13795979915619938No Hit
CCTCCAGGGACTTTGGAGCACTCTGTGTGAAGCTGTTTGGTGGAACCCATGC820.13154306431172497No Hit
TTAGTGGCGGGGTGGCTCTTTCACTGGCAATTCTCAAATGATATACATTTAA810.12993888060060638No Hit
CAGCCTGGGGACGCTCTTCCGATCTCGTAGTGGGAGAGGATGATGTAATTAA800.1283346968894878No Hit
GCTTATGGGAGGGTATTCATATTGTCTTATTGCTGGTACAGTAGGACGTTTG790.1267305131783692No Hit
GGGTCTGGGATAAATACATTAAGTTTAACTGAAATTAATAGTCAAATTTATA790.1267305131783692No Hit
ACTGTTGGGATCACAGGAGTCGGCCAACCTTTTCATAAAGGATCAGTTAGTG790.1267305131783692No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCCTGTCTCTTATAC750.1203137783338948No Hit
GCCTGGGACCATACCATCTCCAGAATTGTTTGGCTTGTTTTCTTTCCTAAAT730.11710541091165759No Hit
TTCCATGGGAGGTCACAGCATGGAAAATGGCTTGGACAGGGGGCAAAGAATG730.11710541091165759No Hit
TTACATGGGGGCAAAACTTATTTTTTTTTCTGGACAGATCAGATTTCTAGAG720.115501227200539No Hit
GCAACTGGGATGCAGGATTCTCTTTGAAAATTCTTGTATGTCCAGAACAAAA720.115501227200539No Hit
CGCCCGGGTTGTAATCCATGGAGCAATAGTTGGTTTAATTAAATTATGGGAC720.115501227200539No Hit
AACTAGGGGGCAAAGTGAGTATGTAAATGATGGAAGAAAATGTTGGCATCTG710.1138970434894204No Hit
TACGAGGGGACTTGATGTCCTGCTTTTTATCTTTTTAAATACAGATTTTTTT700.11229285977830182No Hit
GGATGTGGGATGCTGGATGTCATGCAGGACCACTTCAGCCGCGCGTCCAGCA700.11229285977830182No Hit
GGTTATGGGATTGGGAAAGAGCAAACATCTGTTTCTACCCCATCTCATTTCA700.11229285977830182No Hit
ATTGTTGGGAATTAAAAAATATGCACATTCAAGAGCACAGGAAGTTTGAACT690.11068867606718322No Hit
GCCTCGGGTGGGCCCAGGAAGGCAGTTGAGACTTCATGCACTTTTGGGCAGA690.11068867606718322No Hit
AAACAGGGGAGTTCCTGATATTACCACTGAATTTCAAAAATTTCAGTGGTAA680.10908449235606463No Hit
TACCGCTGGGGCATACCTCAGAGATGTCCTGGGTTTAGTTTCAAACCACCAA680.10908449235606463No Hit
AAAGCAGGGAGGGATCTTTACAGAGAGCAAAGCCTGATTACACTGATACAGG670.10748030864494602No Hit
AAGATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA670.10748030864494602No Hit
GGTGTCGGGGAAGGGGAAAGGGAAATCAAATTAATTTTTCTCGTTAGACATA670.10748030864494602No Hit
TGACCAGGGGAAAACAACTGACTATGATGATAGACAGCTCCTGATTGGCAAA660.10587612493382743No Hit
GCGCTTGGGGTTGAAAACTCCAGAAGAAATAAAGCTTTTCTTTGATTTCTCA650.10427194122270883No Hit
GTAAGTGGGCAGATTAAGAAAGTAGAGAAGATGTAAGTTTATGTTTCCCTTT650.10427194122270883No Hit
TGTGTTGGGAAGAAATAGATAACCTGAAGATCCCTATGTCTATTTTAAAAAT650.10427194122270883No Hit
GCATCGGGGATATCGTTATGCTTGCCTCTTATTTGCTTGATTCTCCAGGTAT650.10427194122270883No Hit
TACACAGGGAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTT650.10427194122270883No Hit
AATGCTGGGCAGAGTAACACTTTAATTGTAAATAAATTATTATTTACAATTG630.10106357380047164No Hit
ATACGAGGGACTGCTGTTTAGAAAATTTAAAAGTTAATGAACTTACGAAAAA630.10106357380047164No Hit
CGTGCGGGGATAGAACTGGTGTTGTTTGCTGTTTTTATTTCTCTAATTGTTG630.10106357380047164No Hit
ATATGCGGGAGTGCCCGGACGGGGGGCTCTGCGCTCCGCCCCTCAAGTCCCC630.10106357380047164No Hit
GGACTGGGGATGCTAAAAAATTGGGTAACTGAGATGAAATCAGAATTTGTGG630.10106357380047164No Hit
TCATGAGGCGATCTTCAAGCGGGATCTGGTTGTGACTCTAAGCTCAGTGCTC630.10106357380047164No Hit
TATGGCAGGGATTTGGCTGGAGGAGAAAAGAATATTAAAGTGTCATGGACAT630.10106357380047164No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)