FastQCFastQC Report
Sat 31 Dec 2022
SRR5849724.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849724.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61718
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT6971.1293301792021777No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC5950.96406234809942No Hit
CTCTAAGGGAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCT1610.2608639294857254No Hit
GCTGCTGGGAGGACTCACTGCCCTCCAGAAAACAGAGGTGACAAATGTGAGG1610.2608639294857254No Hit
GCTGATGGGCAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT1520.24628147380018794No Hit
CAAGTGGGGAGTCTGAAACAGCAGCTTCTGAGGAAACCTTGTTCAACGCGTC1510.24466120094623933No Hit
GTGGTGGGACCTATAAATGGACTTGCTTCTTGTGTTTGAAACACTCGTGTGC1430.2316990181146505No Hit
GCAATAGGGAAGTTTCTCAGAGAAACAGTGTATGTGATGGCAGGACCCCAGT1410.2284584724067533No Hit
AGTCAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1370.22197738099095887No Hit
TCAGAAGGGAGTGTGGCCCGGGCGTCCGCTCCTCAGCGGATGTGGCAGCCCC1190.192812469619884No Hit
TGCTAGGGGTCGGTATGTTCAAACTCAGACACGAAAACCAGAACTGCATCAG1190.192812469619884No Hit
GCTCGGGATGGTGCAGACATGGCCAAGTCCAAGAACCACACCACACACAACC1140.18471110535014096No Hit
CATATTGGGGCTCGCGGCGCTCGAGGGACCATGGCCGATCCTCGCGTGAGAC1080.17498946822644934No Hit
CAATGAGGGACTCTTATAGCCTGCCTTCAATTTTGATCCTTTATTCTTTCTA1040.1685083768106549No Hit
AAGCTAGGGTTATAATAAGATTAAGAGACATAATAATAAAATGCAATGTATT1030.1668881039567063No Hit
CTTATGGGGATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAG980.15878673968696327No Hit
AATACGGGGAAAGAAGGGGACATCATTACATTAACCAATCAAATAGATGAAA950.15392592112511747No Hit
CTACGCGGGATTGTCCTGGGTGGACCAATCCAAAGGCTAAGTGATAGTGACT900.14582455685537443No Hit
GCAACTGGGATGCAGGATTCTCTTTGAAAATTCTTGTATGTCCAGAACAAAA860.13934346543958No Hit
ACGAGGGAAGGGATTCTCCTGCCTCAGCTTCCTAAGTAGCTGGGATTACAGG840.13610291973168281No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATG810.131242101169837No Hit
TGTTACGGGAGGCCGTGGAGCTTGCCGTCCCACCTCCGTCCAAATCGACCTT790.1280015554619398No Hit
GCCTGGGACCATACCATCTCCAGAATTGTTTGGCTTGTTTTCTTTCCTAAAT780.1263812826079912No Hit
GGACACGGGACTGGGGGTCTTGAAACATATCCTCTCAGAATTGATTATTATT770.12476100975404257No Hit
TTACATGGGGGCAAAACTTATTTTTTTTTCTGGACAGATCAGATTTCTAGAG770.12476100975404257No Hit
GAAACGGGGGGTTTCTAATTGTGAGAACTACCCTTCCATCAAACAAAAAGGG770.12476100975404257No Hit
GCATCGGGGATATCGTTATGCTTGCCTCTTATTTGCTTGATTCTCCAGGTAT750.12152046404614537No Hit
TTGTTCAGGGCTATGGAAACTCATCTGATTCCAGATATGATGATGGAAGAGG750.12152046404614537No Hit
CAGCCTGGGTGACAGAGTGGGATACCATCTCTTCAGAAAAAAACAAAGATTT750.12152046404614537No Hit
CAGCCTGGGGACGCTCTTCCGATCTCGTAGTGGGAGAGGATGATGTAATTAA730.11827991833824815No Hit
GCTTCAGGGCAAGACTAATAAGATGCCTAAAAACCCTTAAACCCTAATTCAT730.11827991833824815No Hit
GTTATGGGGGACCGAATCTGAAACTTTGGGAATTTGCTTTAGCCAGAATAAA720.11665964548429955No Hit
TCGCCTGGGTGCCTGTAGTTCTTCAAGGAGTGGTACAATTTGGGTAGGAAAA720.11665964548429955No Hit
CATAATAGGGTGGCTTCAAAGAAGAAACTAAAAGCCACCTCTTGCAGGAAAC720.11665964548429955No Hit
AGTAATGGGAATGACTGGAAAGTAAAGAGGACCAAGGTGAAAATAAAGGCTC710.11503937263035095No Hit
TACACAGGGAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTT710.11503937263035095No Hit
ATGTTGGGGCTGAATGTGGCGGGCCAAACTGCAGAACTGACGAAGGAGAGAG700.11341909977640235No Hit
ATTGTTGGGAATTAAAAAATATGCACATTCAAGAGCACAGGAAGTTTGAACT690.11179882692245376No Hit
TAGTTGGGAATCCAATCACATTGATACCTTGTTTTATTTCATCATAATTTGA690.11179882692245376No Hit
GGTAATGGGGCGAGGGACCAATACACATGTCCTATTTAATGAAATTAGATTG690.11179882692245376No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCCTGTCTCTTATAC690.11179882692245376No Hit
GGTTATGGGATTGGGAAAGAGCAAACATCTGTTTCTACCCCATCTCATTTCA690.11179882692245376No Hit
AAAAAAGGGAATGAACTGAATAATCCAGAGCCTTTTCCCACCATTCTTACTA690.11179882692245376No Hit
TGTGATGGGATGATGTCCTGAGCTTGTTTTCCCCTATCGTGTTACTGTCACT680.11017855406850513No Hit
CTTGGACTGGGAAGTTCGAGGTGGCTACCGATTTCAGCACATGCACTACTGA680.11017855406850513No Hit
AAACCTGGGATCTGGCATAGGGAAATGCAGCAACTTGTTTTAAAAAAATGCA670.10855828121455653No Hit
GACAGTGGGGAGGTGGGAGGTTAGTGTGGCCCGGGCGTCCGCTCCTCAGCGG660.10693800836060793No Hit
TTTGTCCGGGAGGCTTTCTTTCATGCACTTTACTCATAGCACATTTCTTGTG650.10531773550665934No Hit
TAGGTAGGGACCAAAAAAAAGTAAGTAAACTAAAACACAAAAACATATAAAT650.10531773550665934No Hit
CACAACGGGGGAGTTTCAGCTAATGAGGTGAAGGCCACAGTGCCTCTTAAAC640.10369746265271071No Hit
CCTCCAGGGACTTTGGAGCACTCTGTGTGAAGCTGTTTGGTGGAACCCATGC640.10369746265271071No Hit
AATGCTGGGCAGAGTAACACTTTAATTGTAAATAAATTATTATTTACAATTG640.10369746265271071No Hit
ATACGAGGGACTGCTGTTTAGAAAATTTAAAAGTTAATGAACTTACGAAAAA640.10369746265271071No Hit
CATGCTGCAGGGAGAGTGGGCAGTGATATGACCTCCCAACAAGAATTCACTT640.10369746265271071No Hit
GCTTTAAGTTTTTGGAATTTGAGTACAAGGAAAAAGAAGAACAAAAATAATA640.10369746265271071No Hit
AATTAAGGGGAATTGATGAAGTGGAAGAAAGGATATCAGAGATTGAAGTCAG630.10207718979876211No Hit
TACCGCTGGGGCATACCTCAGAGATGTCCTGGGTTTAGTTTCAAACCACCAA630.10207718979876211No Hit
CATGCAGGGAAAGCAGTAGGCAGAATGCTCAGATGTATGTTTAACAGCTGTG620.10045691694481351No Hit
AAACCGGGTGTCATAATCAAATGAGAATGTGGAATACCCAAACTCTCTGTTT620.10045691694481351No Hit
TGCTTAAGGGACTCTTAAGAAGGGCCAGTGTATAGCTGACTCTACATATAAT620.10045691694481351No Hit
GACGCTAGGGGGTATCAGTGTTTTTAAAAAGTGTTTGAAGTGATTGAAATGT620.10045691694481351No Hit
ACTGTTGGGATCACAGGAGTCGGCCAACCTTTTCATAAAGGATCAGTTAGTG620.10045691694481351No Hit
TTCCATGGGAGGTCACAGCATGGAAAATGGCTTGGACAGGGGGCAAAGAATG620.10045691694481351No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)