FastQCFastQC Report
Sat 31 Dec 2022
SRR5849723.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849723.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61461
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT6391.039683701859716No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC6090.9908722604578514No Hit
GCTGATGGGCAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT1670.27171702380371293No Hit
CTCTAAGGGAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCT1510.2456842550560518No Hit
TGCTAGGGGTCGGTATGTTCAAACTCAGACACGAAAACCAGAACTGCATCAG1460.23754901482240773No Hit
GTGGTGGGACCTATAAATGGACTTGCTTCTTGTGTTTGAAACACTCGTGTGC1420.23104082263549242No Hit
CAAGTGGGGAGTCTGAAACAGCAGCTTCTGAGGAAACCTTGTTCAACGCGTC1380.22453263044857713No Hit
AGTCAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1220.19849986170091602No Hit
GCTGCTGGGAGGACTCACTGCCCTCCAGAAAACAGAGGTGACAAATGTGAGG1190.19361871756072957No Hit
AAGCTAGGGTTATAATAAGATTAAGAGACATAATAATAAAATGCAATGTATT1100.1789752851401702No Hit
CTACGCGGGATTGTCCTGGGTGGACCAATCCAAAGGCTAAGTGATAGTGACT1090.17734823709344136No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATG1090.17734823709344136No Hit
GCAATAGGGAAGTTTCTCAGAGAAACAGTGTATGTGATGGCAGGACCCCAGT1080.17572118904671255No Hit
CTTATGGGGATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAG1070.17409414099998372No Hit
CATATTGGGGCTCGCGGCGCTCGAGGGACCATGGCCGATCCTCGCGTGAGAC1030.16758594881306846No Hit
GCTCGGGATGGTGCAGACATGGCCAAGTCCAAGAACCACACCACACACAACC1010.16433185271961082No Hit
CAGCCTGGGTGACAGAGTGGGATACCATCTCTTCAGAAAAAAACAAAGATTT990.16107775662615317No Hit
CAATGAGGGACTCTTATAGCCTGCCTTCAATTTTGATCCTTTATTCTTTCTA940.15294251639250908No Hit
TCAGAAGGGAGTGTGGCCCGGGCGTCCGCTCCTCAGCGGATGTGGCAGCCCC920.14968842029905144No Hit
AATACGGGGAAAGAAGGGGACATCATTACATTAACCAATCAAATAGATGAAA880.14318022811213615No Hit
GGTTATGGGATTGGGAAAGAGCAAACATCTGTTTCTACCCCATCTCATTTCA870.14155318006540732No Hit
CAGCCTGGGGACGCTCTTCCGATCTCGTAGTGGGAGAGGATGATGTAATTAA860.1399261320186785No Hit
TACCACTGGTCTAACTTATTGCCATGTTTATTGACTCTTGGCATATATATTT840.13667203592522087No Hit
ACGAGGGAAGGGATTCTCCTGCCTCAGCTTCCTAAGTAGCTGGGATTACAGG820.13341793983176323No Hit
GCTTCAGGGCAAGACTAATAAGATGCCTAAAAACCCTTAAACCCTAATTCAT800.13016384373830558No Hit
ATACGAGGGACTGCTGTTTAGAAAATTTAAAAGTTAATGAACTTACGAAAAA790.12853679569157678No Hit
GCAACTGGGATGCAGGATTCTCTTTGAAAATTCTTGTATGTCCAGAACAAAA780.12690974764484794No Hit
TGCACGGGAGTTAACAACGGACTTGGGAAATCTCAGGCTAAGAACAACAAGA760.1236556515513903No Hit
AATGCTGGGAGGAGAAATTAGAAAGCCACTCACATGCCCAGGAAATATGCAT750.1220286035046615No Hit
AGTAATGGGAATGACTGGAAAGTAAAGAGGACCAAGGTGAAAATAAAGGCTC720.11714745936447504No Hit
TTACATGGGGGCAAAACTTATTTTTTTTTCTGGACAGATCAGATTTCTAGAG720.11714745936447504No Hit
GCACAAAGGGAAAGACATCGCTTCTAGTAGTCACTAAAAGCTGTAGTAGATG710.11552041131774621No Hit
AAACAGGGGAGTTCCTGATATTACCACTGAATTTCAAAAATTTCAGTGGTAA710.11552041131774621No Hit
TGACCAGGGGAAAACAACTGACTATGATGATAGACAGCTCCTGATTGGCAAA710.11552041131774621No Hit
GGACACGGGACTGGGGGTCTTGAAACATATCCTCTCAGAATTGATTATTATT700.11389336327101739No Hit
TTGCTTGGGTGTATAATAATCATCACAATCAGGAATTCTTTTATTCAATGTA700.11389336327101739No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGAACTCGTATGC700.11389336327101739No Hit
CCTCCAGGGACTTTGGAGCACTCTGTGTGAAGCTGTTTGGTGGAACCCATGC690.11226631522428857No Hit
TGCAGTTGGGATGTTGTCCTGGTGTCTTGATTTTTTGGTTCATCTGTTTTAA680.11063926717755974No Hit
AAGCCTGGGTGACAGAGCAAGACTCCATCTCAAAAATAAAAATAAAATAAAA680.11063926717755974No Hit
GTAACAGGGTAAGCAGGGAAACCAACACATTCATCCATGAGGATTTTGGTCC680.11063926717755974No Hit
GCTTATGGGAGGGTATTCATATTGTCTTATTGCTGGTACAGTAGGACGTTTG660.1073851710841021No Hit
CACAACGGGGGAGTTTCAGCTAATGAGGTGAAGGCCACAGTGCCTCTTAAAC660.1073851710841021No Hit
ACTGTTGGGATCACAGGAGTCGGCCAACCTTTTCATAAAGGATCAGTTAGTG650.10575812303737331No Hit
CGAATAGGGGATCTCTCTTGGGGTATGGCATTCCCCAACTTCTCTTAAGTGG650.10575812303737331No Hit
AAAGCAGGGAGGGATCTTTACAGAGAGCAAAGCCTGATTACACTGATACAGG640.10413107499064449No Hit
TGTTACGGGAGGCCGTGGAGCTTGCCGTCCCACCTCCGTCCAAATCGACCTT640.10413107499064449No Hit
TTCCATGGGAGGTCACAGCATGGAAAATGGCTTGGACAGGGGGCAAAGAATG640.10413107499064449No Hit
TACGAGGGGACTTGATGTCCTGCTTTTTATCTTTTTAAATACAGATTTTTTT640.10413107499064449No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGA640.10413107499064449No Hit
AATGCTGGGCAGAGTAACACTTTAATTGTAAATAAATTATTATTTACAATTG640.10413107499064449No Hit
CGCGTAGGGCTTGGCTTTTTAAATTTTTTCTGTCACAGCACACCTGAAGTTC630.10250402694391567No Hit
ATGTTGGGGCTGAATGTGGCGGGCCAAACTGCAGAACTGACGAAGGAGAGAG630.10250402694391567No Hit
GGTGTCGGGGAAGGGGAAAGGGAAATCAAATTAATTTTTCTCGTTAGACATA620.10087697889718683No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)