FastQCFastQC Report
Sat 31 Dec 2022
SRR5849707.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849707.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62635
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC2760.4406481998882414No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT2620.4182964796040552No Hit
GGTTGCGGGGATCGGAGCGGAAGTGGAGCTACCGCCACCGCCGCCGCCGATT910.14528618184721No Hit
CCAGATGGGCAAAGAGAACTAACTTTGGCTTATTCATATTCTGTTCAAAGAC910.14528618184721No Hit
AATGCCGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA910.14528618184721No Hit
ATTTTTGGGGGGTATTTTCTCATTTCCAAAGACCCAGAGTCTCAGAGCCAGG890.14209307894946915No Hit
TGAACAGGGATTTGTTTTCTGATTATCTCTTTTTTGAGGGGTTTCTTCTGTT850.13570687315398738No Hit
GTAAACGGGGATATGAAAACTGTTGGAGATACAAGAAAACAGACACAGAAAT840.13411032170511694No Hit
GTACAAGGGGTGGCCTCATGTGGCCTTGCCTCTGTGTGTGGCACACCTGATG830.1325137702562465No Hit
GAGAGAGGGACTTGCTTTGGTGATTGCAATCAGCATGGGATTTGGCCATTTC820.13091721880737608No Hit
TCTGAAGGGACATAAGGGCTTGTGATTATTATTACTTGAAACAGGAGAGCTA820.13091721880737608No Hit
CAGTCCGGGAACCCGTCTCTGCTAAATATACAAAATTAGCCAGGTGTGGTTG820.13091721880737608No Hit
TAATGGGGGGGCCTTCCAATGTGTGAGATTTGAGACTAGAGATTCAAATTCA810.12932066735850564No Hit
TAGAATGGGATTTCTAAGTGGGTAAAAAATACTCTTACAGAATTAAGTGAAT810.12932066735850564No Hit
ATCCCAGGGGCGGTCATGCAAGTCTTCACTGAGTGGCTGCGGGACGGCCACG810.12932066735850564No Hit
TCATGCGGGAGAAAGTTGTTGATTTAATATGAAAGTTTAGAAAAGATAAGGT800.1277241159096352No Hit
AGCATTGGGTGCCTACTGTTTCACCAGTGCAGTGGAAGAGGAGAAAATGGGC790.12612756446076476No Hit
GAAATTGGGAGTTGAAATTCCCAAGAACAGGAAAAAGATGATGGCTCAGAAA790.12612756446076476No Hit
CGACAAGGGAAGAACTTGAATTTCTGGAAGATTCTTACTGTTAACCAAATTT790.12612756446076476No Hit
GTAGCTCGGGATCCGAGCCTTCCGGGAAGAAGCCATTAAGTTTTCAGAAGAG780.12453101301189431No Hit
TATGCCGGGCAAGAGAGAAATAATAAATGCTATTCTGAACTTTATTTCCCAA760.12133791011415343No Hit
ACGTTGGGGATCCAGTTGAAATCATATAAATTTTTCAGGTACTCAGTTTCAC760.12133791011415343No Hit
GGCTTGGGAATTCAAGTTTAGAAACAGGTAATCAGCTATTTGATCTTAAAAG750.11974135866528299No Hit
GAAACGGGGAATACCAAGGAATTTCTCAAGATCAGCTCATTTGCTCTCTACA750.11974135866528299No Hit
ATCTTGCGGGATGTCATAATGAGTGAACCAGAACTTACAATTTTGTGAATGA740.11814480721641255No Hit
AAACAAGGGATGCTACCATGTTGGATAAAGAAGCCAAACCATACAAAGTTGA740.11814480721641255No Hit
TGCCAAGGGAGGAATGAAGAGAGAAAGCAGCTCCCTAACTTCAAAACCATTT740.11814480721641255No Hit
TGTCCAGGGGCAGTGAAGTAAGCTTGACATTTGGTTTCCTTTCCTCTTAAGG740.11814480721641255No Hit
GTAATAGGGAGGGGCTTTCAAACAAGCATCCACCAAGTGTTAAATTAAAGGA740.11814480721641255No Hit
TATCCGGGGATTGCTTAATTCAGTAGACATTGTTTTTTCTTCTTCTCATCTT730.1165482557675421No Hit
GCACTTGGGTCAAAGAGAATGTGCATTCTCTGTTTATAAGGCTCAAACTTAT720.11495170431867166No Hit
TACAAGGGAATAATTTAAAACTACTATAGAAACTGCAGAGCAAAGGAAGTGG720.11495170431867166No Hit
TTTCCAGGGATGCACACTTCCCAGCTTGAACTTGAACATTTGTTTCTGTCTT720.11495170431867166No Hit
ACCGCAGGGGGCAGACTTCGGTTGAGGAAACGGGTATTTCATGTCTCAGGGA720.11495170431867166No Hit
GCGCTGGGGTGAGCACGGGATATTAACTCTTTTGGAAACATGAACTCTTACT710.11335515286980122No Hit
GTGTGTGGGACTGGCCTGTGCCAAGACCATGAACGATTTTGATTTTTACTTT710.11335515286980122No Hit
GTTCCAGGGTAACTCACTGTTAGACAAGTAGGTAATGTGTTCCCTCAGCGAT710.11335515286980122No Hit
TGAACGGGGACTGTGCCTGAGCTCTGGAGTGCCAAGGCCTACCCATCGCTCA710.11335515286980122No Hit
AAGTCCGGGCAGGGCCTTCATTTTTGCTCCTCTCATCTCCAACTCATGTTCA710.11335515286980122No Hit
ATTGCAGGGTTGATAGTGGAATTATATATTTTTACTCTATGTTTCTCTACAT700.11175860142093079No Hit
TCACACGGGCAATGGCAACCATGTACACTGCACTCTGAAGAAAGTACTGTGT700.11175860142093079No Hit
AACTGCGGGAGGGCTTCTGATTTCTTACTGATTGGTTTCTAATCTCTGTGTT700.11175860142093079No Hit
AGCTCCGGGATCATGGCAAATGGCCCATACTAATTAAGGCTTTGCTAATTTA700.11175860142093079No Hit
GCTACATGGGGGAAAATGGAAAATATTGAATTATTTCTTTTGCATTTGCATA700.11175860142093079No Hit
TAAAATGGGGTGTGAGAAGTTGTCTTGGATTGGACTTGCTCTCCAACTGGCC700.11175860142093079No Hit
TATTATGGGGAGCTGTCGGAAGGTGGCGAGCCTGAGGCGAACAATGGCGGAG700.11175860142093079No Hit
GTTTGTGGGTTTGCCTTTCATGTATCCCAGATACTGCTCTGGGAAGTCTGCA700.11175860142093079No Hit
TAGTTTGGGGGAGTAATTTTGATTAGTGGAAAAATAAAGCCCACCAATGGCA700.11175860142093079No Hit
CTTGCGGGGACTGTGATGTCATTAACTTCTTTGAAATGCTTCATATACAACT690.11016204997206035No Hit
CTTTGTGGGATTCTAATTACATTTAAAATGTATGGATAGGACAAAAAAAGAA690.11016204997206035No Hit
ATAGTAGGGACTTGACCCTTGTGCTTGGCTCTGACGTGCTTACTCTGAAACT690.11016204997206035No Hit
GTAACCGGGTCCAGTGGTTTGTGAGGATCACATGATGGAATGGAGGTGAAAT680.1085654985231899No Hit
AAATACGGGATTGCCTGAGAACTTAGTTCAGCCTTTGCTAATGCCAAAATGC680.1085654985231899No Hit
TTATTTGGGATGATTTTTTAGTGAGTCACTTTGGGAAGGTGATGATCAAGAA680.1085654985231899No Hit
AGTCAGGGGGATTAACATTTTTTACAAATTTGCCTGCATAAAGATTAAATTG680.1085654985231899No Hit
GCCATGGGGAAAAAAGGAATAAAGAATAAGGAATCAAACAGAAAAGAAATAA680.1085654985231899No Hit
CAGCTAGGGACTGTTGACAGTTTCCGCAGAAATCCTATTTCAGTGGACCAAC680.1085654985231899No Hit
GGCTCAGGGACTTAGAGTGACAAATAATTATTTTTCAAGGATAAATTGAGCA670.10696894707431946No Hit
GACACCGGGAACTTTAGACGGTCTTGTATCAGTGGTGGTCTCTTGAATTTTG670.10696894707431946No Hit
TCACAAGGGATTCCACAAGGATAAGTGCTTCCTGCTGGGACAAAGTCCTCAT670.10696894707431946No Hit
CTCCAAGGGATTGAAATCTTGTATTATAGCAGGACTCCTCTGACATCACTCT660.10537239562544902No Hit
GCTCTTGGGGAGCTCCAGTCTTTTTGAGAGCACCAGTGGTTTCTAGTTGTTT660.10537239562544902No Hit
CATACAGGCGGCTAAAGGGAGCTGGCCTAGGAGCCAAAGGCAGCGCATATGG660.10537239562544902No Hit
CCATGCAGGGAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAA660.10537239562544902No Hit
GTCATGGGGAAGATGAAATTTAAATAAAAAAGGACAGAGTCTATCCTCCAGG650.10377584417657858No Hit
CAGATAGGGGACAGTTAAATGCTAATTAATATACATCTGTAGTTATTCTACA650.10377584417657858No Hit
ACTATGGGGAACTTCACAGGACGTGGAACATGTAAATGCATTTTGAGTTGAA650.10377584417657858No Hit
CACAATGGACTATTGTTCTAGGTATACTGAGGATTAAAACTGACAGAACAGA650.10377584417657858No Hit
GTGCCAGGGATTTTAAGTAGAGGCGAGGTTTCTCCATGTTGGTCAGGCTGGT650.10377584417657858No Hit
TTCTGTGGGGATGGGGATGGTTCTTCTAAATGAAGCAGCAACTTCGAAAGGC650.10377584417657858No Hit
TACGCTGGGGAAGGTACTTCAGCTTAATCTGAAATATAATTTAACTTGTGAA650.10377584417657858No Hit
TATCTAGGGAACTCGTGTAGTTGTCTGGTTTCAGGGATAGCTGTTTGAATTG650.10377584417657858No Hit
TGCTATGGGACCTTCACAGCAACAGCTGACTGGGATTTGACGGAACAACTGA650.10377584417657858No Hit
CATCATAGGGGCTCTTCCGATCTCGGCATGGGAGTAAATCAAATCCAACAAT650.10377584417657858No Hit
AACTCAGGGATTACATATATGCAATACACAAGTTATAACTGACCTTATACAC650.10377584417657858No Hit
TTAGATGGGAGGAAAGGTTCAATTTAGCATCCTGTTGACAGTGCTAAATAAC640.10217929272770816No Hit
CAATTCGGGATATTAGTAGGTCAAAGGAGAAAAATGATATTATTTTCATTGG640.10217929272770816No Hit
GACTAGGGGGTTTTCTTCCATCCTGTCAGTTGCCTTTTTACTCTATTATAAT640.10217929272770816No Hit
TCATGAGGGATGTGGAGCTGTGAGTCAATTAAACCTCTTTCCTTTATAAATT640.10217929272770816No Hit
GTAAGCGGGCCAGGAATGAGCTTGGCCTCCAACATCCCTTGCCTGCTGCCAT640.10217929272770816No Hit
AACATGGGGATAGGTTTAAAAAATATATAGAAATGAAATAATGCATCTGTCA640.10217929272770816No Hit
TAAGCTGGGGTATACAATGTCTTAACAGACTTCTCCTCCCCTGTATCTGAAT640.10217929272770816No Hit
TCTCAAGGGATTTCAGCCAAGCAAGGGAAAGAGGAAGTGGAGTCATTTTGCC640.10217929272770816No Hit
CCCATGGGGATAGAGTGACTGGCATGGGGAATGGGTGGTAGAGGTCAAATGA640.10217929272770816No Hit
TAAGCCGGGGTATAGTAAGAGATGGATGAGGAAGAATTTTCAAACTTGAGGG640.10217929272770816No Hit
TGCGTGGGGGAATTTAACTACATTAGTATACAGTTAGGTTTTTTTTAATCTG640.10217929272770816No Hit
TAGGCTGGGAGTACAGGGAATATAAAATTGGTTTCCTGTGGTCATGATCAAG640.10217929272770816No Hit
TTGGCCGGGTGCCTTGTCTCCAGTGGTTTGTCCCTCTGGTGCCTCAGCAGAT630.10058274127883772No Hit
TAATGCGGGATACAAAAAGTTCGCTGACCCCTCTAATAGATGTTACACATAC630.10058274127883772No Hit
GCATTTAGCGAAAACATTTTGTTTTGAAAGTGTGTTCTTTTTGTCGCACTGT630.10058274127883772No Hit
TTGCATGGGGAGGTGCCTGGAGATCTTTAGCAGGCAGTTATGTACTCAGATG630.10058274127883772No Hit
CTTGAGGGAGATTTCACAGACTACATTAGGTTAGATTTGAAAGAAAAATAGG630.10058274127883772No Hit
TGCTCTGGGACATGTGGTAGGCGGCCCTGTGGTGGGGACTTGTGCCTCCAGG630.10058274127883772No Hit
TGTGGCGGGGAAAGGAAGTTTGGGCTAGACCATGTGGCGACCACAGACCAGG630.10058274127883772No Hit
GCTTCGGGGACTCCTGAAAGTTAAATCAGAAGACACTGATTTCATTTTGTGA630.10058274127883772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)