FastQCFastQC Report
Sat 31 Dec 2022
SRR5849706.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849706.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62728
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC2600.4144879479658207No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT2290.3650682310929728No Hit
ACTATGGGGAACTTCACAGGACGTGGAACATGTAAATGCATTTTGAGTTGAA990.1578242571100625No Hit
GGTTGCGGGGATCGGAGCGGAAGTGGAGCTACCGCCACCGCCGCCGCCGATT980.15623007269480932No Hit
GTACAAGGGGTGGCCTCATGTGGCCTTGCCTCTGTGTGTGGCACACCTGATG970.15463588827955618No Hit
GCGCTGGGGTGAGCACGGGATATTAACTCTTTTGGAAACATGAACTCTTACT940.1498533350337967No Hit
ATCTTGCGGGATGTCATAATGAGTGAACCAGAACTTACAATTTTGTGAATGA940.1498533350337967No Hit
ACCGCAGGGGGCAGACTTCGGTTGAGGAAACGGGTATTTCATGTCTCAGGGA940.1498533350337967No Hit
CAGTCCGGGAACCCGTCTCTGCTAAATATACAAAATTAGCCAGGTGTGGTTG920.1466649662032904No Hit
GTTGGCGGGTCACTAGAGACAGAAACTAAAAACCATGGCTTCAGGCTGCTAA880.14028822854227777No Hit
TGTCCAGGGGCAGTGAAGTAAGCTTGACATTTGGTTTCCTTTCCTCTTAAGG870.13869404412702463No Hit
CCAGATGGGCAAAGAGAACTAACTTTGGCTTATTCATATTCTGTTCAAAGAC850.1355056752965183No Hit
TCTGAAGGGACATAAGGGCTTGTGATTATTATTACTTGAAACAGGAGAGCTA850.1355056752965183No Hit
TTCCACGGGATAATTACTTTTTTAAAGTTTCATGTACCTAAAGCATATGCTA850.1355056752965183No Hit
CACAATGGACTATTGTTCTAGGTATACTGAGGATTAAAACTGACAGAACAGA840.13391149088126514No Hit
TCATGCGGGAGAAAGTTGTTGATTTAATATGAAAGTTTAGAAAAGATAAGGT840.13391149088126514No Hit
AGCTCCGGGATCATGGCAAATGGCCCATACTAATTAAGGCTTTGCTAATTTA810.12912893763550568No Hit
TAGAATGGGATTTCTAAGTGGGTAAAAAATACTCTTACAGAATTAAGTGAAT810.12912893763550568No Hit
GTGCCAGGGATTTTAAGTAGAGGCGAGGTTTCTCCATGTTGGTCAGGCTGGT810.12912893763550568No Hit
TCACAAGGGATTCCACAAGGATAAGTGCTTCCTGCTGGGACAAAGTCCTCAT800.1275347532202525No Hit
TATCCGGGGATTGCTTAATTCAGTAGACATTGTTTTTTCTTCTTCTCATCTT770.12275219997449306No Hit
GTAATAGGGAGGGGCTTTCAAACAAGCATCCACCAAGTGTTAAATTAAAGGA770.12275219997449306No Hit
TAACCAGGGATCTGTGTTAGGGCAGCAGATTGTGAAGCACAAGTGGAAGCTG770.12275219997449306No Hit
TGAACAGGGATTTGTTTTCTGATTATCTCTTTTTTGAGGGGTTTCTTCTGTT760.12115801555923988No Hit
GTAAACGGGGATATGAAAACTGTTGGAGATACAAGAAAACAGACACAGAAAT750.11956383114398673No Hit
AACTGCGGGAGGGCTTCTGATTTCTTACTGATTGGTTTCTAATCTCTGTGTT750.11956383114398673No Hit
TCTATTGGGCTGTCATTTAAGTTATGTAAAAAATTTAATCATTATTTTGATG750.11956383114398673No Hit
CAGCTAGGGACTGTTGACAGTTTCCGCAGAAATCCTATTTCAGTGGACCAAC750.11956383114398673No Hit
TTTAAAGGGAGTGCTGCAGAACTTCGCCATGTGATGACAAACCTTGGAGAGA740.11796964672873358No Hit
GAGCAGGGAGCTGGGACATGTGTGCGCCACCACTCTCCCCTAGTTTTTGTGT740.11796964672873358No Hit
ATAGCAGGGACTCCCAAGAGCTGGTGTAGAATGAGTTCCTCTGGCCATGGGA740.11796964672873358No Hit
GAACATGGGACATTTTTCCTGGAACAAGGGAGGGAATAAATAGTTGTCTCCA740.11796964672873358No Hit
TTAGATGGGAGGAAAGGTTCAATTTAGCATCCTGTTGACAGTGCTAAATAAC730.11637546231348044No Hit
TCATGAGGGATGTGGAGCTGTGAGTCAATTAAACCTCTTTCCTTTATAAATT730.11637546231348044No Hit
ATCCCAGGGGCGGTCATGCAAGTCTTCACTGAGTGGCTGCGGGACGGCCACG730.11637546231348044No Hit
TGGAAAGGGATATATTTTGAAGGTAGAACAGACCGAATATGCTGATGAATTA730.11637546231348044No Hit
TACTCTGGGAGCCCAAGCATCTGTTTCACAGCTCTGAAACCTATAAAATGGG730.11637546231348044No Hit
TTCAAGGGACTACTTTTTAATTTAAATACCCAACCTCTCCATCACAGCTGCA730.11637546231348044No Hit
GCTTCGGGGACTCCTGAAAGTTAAATCAGAAGACACTGATTTCATTTTGTGA730.11637546231348044No Hit
GAGAGAGGGACTTGCTTTGGTGATTGCAATCAGCATGGGATTTGGCCATTTC720.11478127789822726No Hit
GTTCTGGGGTCTGCGCGAGGGGGGTTTTAACTTTGTTTTATTTTCAAAACCA710.11318709348297411No Hit
TATAAAGGGGAACCCTTCAAACTAAACTTTAGCCTTTTGGCTGGTGTTGAAT710.11318709348297411No Hit
ACGTTGGGGATCCAGTTGAAATCATATAAATTTTTCAGGTACTCAGTTTCAC710.11318709348297411No Hit
CACTAAGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA700.11159290906772096No Hit
AGCATTGGGTGCCTACTGTTTCACCAGTGCAGTGGAAGAGGAGAAAATGGGC700.11159290906772096No Hit
GTAACCGGGTCCAGTGGTTTGTGAGGATCACATGATGGAATGGAGGTGAAAT700.11159290906772096No Hit
GCTACCGGTTCACTGCTTGACCTTCGCTTCCTCAGCACCTTCAAGCCCCCAG700.11159290906772096No Hit
GTGCAAGGGACATCTACCAAGAAGTAAGTAAGAATAGACTGTTTTTGGGCTG700.11159290906772096No Hit
GAAATTGGGAGTTGAAATTCCCAAGAACAGGAAAAAGATGATGGCTCAGAAA690.10999872465246781No Hit
CTAATGCCGGGATAGTTAAACAGTGATGATCCCAGATATTTGAAGTTTCTTT690.10999872465246781No Hit
ACACAGACAGACATGCCCCAGGGAACAAGTCACATAAACAGAGATGTTCTCC690.10999872465246781No Hit
TGCCAAGGGAGGAATGAAGAGAGAAAGCAGCTCCCTAACTTCAAAACCATTT690.10999872465246781No Hit
GACACCGGGAACTTTAGACGGTCTTGTATCAGTGGTGGTCTCTTGAATTTTG690.10999872465246781No Hit
AAACAAGGGATGCTACCATGTTGGATAAAGAAGCCAAACCATACAAAGTTGA680.10840454023721466No Hit
TTGGCCGGGTGCCTTGTCTCCAGTGGTTTGTCCCTCTGGTGCCTCAGCAGAT680.10840454023721466No Hit
TATGCCGGGCAAGAGAGAAATAATAAATGCTATTCTGAACTTTATTTCCCAA680.10840454023721466No Hit
AAGTCCGGGCAGGGCCTTCATTTTTGCTCCTCTCATCTCCAACTCATGTTCA670.10681035582196148No Hit
TAAGCCGGGGTATAGTAAGAGATGGATGAGGAAGAATTTTCAAACTTGAGGG670.10681035582196148No Hit
GTGTGTGGGACTGGCCTGTGCCAAGACCATGAACGATTTTGATTTTTACTTT660.10521617140670833No Hit
AGATATGGGCTAACCAACAGACTTGGATTGTCTTTTGACATAATCAAAATGC660.10521617140670833No Hit
AGCGTAGGGAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAA660.10521617140670833No Hit
GTTACTGGGATTAGAATCACCTGAGAAGCTTCTAAAACTCCCAGTGCCCAAG660.10521617140670833No Hit
AACATGGGGATAGGTTTAAAAAATATATAGAAATGAAATAATGCATCTGTCA660.10521617140670833No Hit
TATTATGGGGAGCTGTCGGAAGGTGGCGAGCCTGAGGCGAACAATGGCGGAG660.10521617140670833No Hit
TCGTATGGGTGAGTATTCATCAAATCTCAGGTTGAGACAGGAATTCCTATAC650.10362198699145518No Hit
TGATATGGGATTAATAAACTTGAATCAATTACATGTTTATAAGGTACACATG650.10362198699145518No Hit
GTAGCTCGGGATCCGAGCCTTCCGGGAAGAAGCCATTAAGTTTTCAGAAGAG650.10362198699145518No Hit
GTTTGTGGGTTTGCCTTTCATGTATCCCAGATACTGCTCTGGGAAGTCTGCA650.10362198699145518No Hit
AGCTTTGGGACTTCTCAGATGTAATGGTTTCTCCAGGATTCAGAAAGCTGTA650.10362198699145518No Hit
CTTGAGCAGGGAGCTATTCGGTATGTTCAAACTCAGACACGAAAACCAGAAC650.10362198699145518No Hit
AAATGTGGGAACATCCAGAAGGAGTCGACCCTGCACCTGGTCCTGCGTCTGA640.10202780257620202No Hit
GTCTTGGGATCACCGTCTTAATACAAGACACCTTCATCTCTAACTTGTAACA640.10202780257620202No Hit
CTCAATGGGATCTGGACTTGGTCAGGCCAGAGCAGAATCACAGGAGGACTTG640.10202780257620202No Hit
ACTTGAGGGATATCAAGTCTAAATCAAGTTACCCAATCACTAGTAATTAGAG640.10202780257620202No Hit
GTAAGCGGGCCAGGAATGAGCTTGGCCTCCAACATCCCTTGCCTGCTGCCAT640.10202780257620202No Hit
TACGCTGGGGAAGGTACTTCAGCTTAATCTGAAATATAATTTAACTTGTGAA640.10202780257620202No Hit
CTTGAGGGAGATTTCACAGACTACATTAGGTTAGATTTGAAAGAAAAATAGG640.10202780257620202No Hit
CTACGGGGAGTTCTGATTATTAAATATTCTCTGTAAATCAGTAAACATGTAT640.10202780257620202No Hit
TGTGCTGGGGAGACATTCTGGTGATGATTTAGGGGCTTTTTATACACCACAT640.10202780257620202No Hit
GGGACTGGGGTGAGGCTGAGGTAGGAAGATTATTTGAACCCAGGAGTTTGAG640.10202780257620202No Hit
CATCATAGGGGCTCTTCCGATCTCGGCATGGGAGTAAATCAAATCCAACAAT640.10202780257620202No Hit
CGTAAAGGGATTTACAGACATGTAAGCACATCCATTCATTTCAGCAAAATGT630.10043361816094885No Hit
CGACTAGGGGCAAGATTGATGGATCAGCCAACCTAAAAAGTCAGGATGATCG630.10043361816094885No Hit
GTGCCTGGGTGACATGTATATGCCATACCATGAAGCATATGATGTTGTGCAC630.10043361816094885No Hit
TCACACGGGCAATGGCAACCATGTACACTGCACTCTGAAGAAAGTACTGTGT630.10043361816094885No Hit
TCGAATGGGAGAATGAGCAATAAAGGTCAGGGTTAAAAATGGTTTACTGGGT630.10043361816094885No Hit
GAAACGGGGAATACCAAGGAATTTCTCAAGATCAGCTCATTTGCTCTCTACA630.10043361816094885No Hit
CTGCCGGGGACCACTTTGCTAATCAACCCATGAACAAAAAAGTGTCTTGATG630.10043361816094885No Hit
AATGTGGGGATATCAAAAACAGATTCACATTTTGATTGGTGGGAAGGAAAAC630.10043361816094885No Hit
GTTCCAGGGTAACTCACTGTTAGACAAGTAGGTAATGTGTTCCCTCAGCGAT630.10043361816094885No Hit
GCGAATGGGGTGTAGATTTGAATCTTTTTTCTTTGAGTCTGCTTTGAACTTT630.10043361816094885No Hit
GTTTGCGGGATGAAGGTGGTTTATTACAAATAAGCACACTAACCCCTCGGAA630.10043361816094885No Hit
TGCAATGGGCTAGTTTTAGCTTACAGGCCTTAGAAACGAAGTAGTTCCTGCC630.10043361816094885No Hit
ATAGTAGGGACTTGACCCTTGTGCTTGGCTCTGACGTGCTTACTCTGAAACT630.10043361816094885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)