FastQCFastQC Report
Sat 31 Dec 2022
SRR5849705.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849705.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63062
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT2460.39009229012717644No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC2410.38216358504329073No Hit
CAGTCCGGGAACCCGTCTCTGCTAAATATACAAAATTAGCCAGGTGTGGTTG1040.16491706574482257No Hit
TGAACAGGGATTTGTTTTCTGATTATCTCTTTTTTGAGGGGTTTCTTCTGTT950.1506453965938283No Hit
AACTCAGGGATTACATATATGCAATACACAAGTTATAACTGACCTTATACAC910.14430243252671973No Hit
CCAGATGGGCAAAGAGAACTAACTTTGGCTTATTCATATTCTGTTCAAAGAC900.1427166915099426No Hit
GGTTGCGGGGATCGGAGCGGAAGTGGAGCTACCGCCACCGCCGCCGCCGATT890.14113095049316546No Hit
TCACAAGGGATTCCACAAGGATAAGTGCTTCCTGCTGGGACAAAGTCCTCAT890.14113095049316546No Hit
AACTGCGGGAGGGCTTCTGATTTCTTACTGATTGGTTTCTAATCTCTGTGTT850.13478798642605688No Hit
GAAACGGGGAATACCAAGGAATTTCTCAAGATCAGCTCATTTGCTCTCTACA850.13478798642605688No Hit
ATCTTGCGGGATGTCATAATGAGTGAACCAGAACTTACAATTTTGTGAATGA840.13320224540927977No Hit
CGACAAGGGAAGAACTTGAATTTCTGGAAGATTCTTACTGTTAACCAAATTT820.13003076337572547No Hit
CACAATGGACTATTGTTCTAGGTATACTGAGGATTAAAACTGACAGAACAGA810.12844502235894834No Hit
TTGCGCGGTTGAAATTTTTTTGGCGGTGGCATTCTTTTTATTTTAATATATA810.12844502235894834No Hit
AGTCAAGGGATGATGAGGGAATATGATACTTCAAAAATTGAAGCTGCAATAT810.12844502235894834No Hit
ACTAACGGGAGAGATAACCCTGTTAATATTTTTCTGTATTTCTTTTCAGTCT790.12527354032539406No Hit
GTGCCAGGGATTTTAAGTAGAGGCGAGGTTTCTCCATGTTGGTCAGGCTGGT790.12527354032539406No Hit
GGCTTGGGAATTCAAGTTTAGAAACAGGTAATCAGCTATTTGATCTTAAAAG780.12368779930861692No Hit
ATTTTTGGGGGGTATTTTCTCATTTCCAAAGACCCAGAGTCTCAGAGCCAGG780.12368779930861692No Hit
TTCAAGGGACTACTTTTTAATTTAAATACCCAACCTCTCCATCACAGCTGCA780.12368779930861692No Hit
TCTGAAGGGACATAAGGGCTTGTGATTATTATTACTTGAAACAGGAGAGCTA760.12051631727506262No Hit
AACATGGGGATAGGTTTAAAAAATATATAGAAATGAAATAATGCATCTGTCA760.12051631727506262No Hit
TTGCAAGGGAGAGTTGATGAAACCCAATGGGAAAAAGACCAAAACAACAACA760.12051631727506262No Hit
GTTCCAGGGTAACTCACTGTTAGACAAGTAGGTAATGTGTTCCCTCAGCGAT750.1189305762582855No Hit
GAGCAAGGGGGGCTAAGCAGATTGTGTGGAATGGTCCTGTGGGGGTATTTGA750.1189305762582855No Hit
TCTCAAGGGATTTCAGCCAAGCAAGGGAAAGAGGAAGTGGAGTCATTTTGCC750.1189305762582855No Hit
CTGATGGGGTAGACATGTAGCAGTAAAAATAGTTAAAAATGTGGATAGATAC740.11734483524150835No Hit
TTAGATGGGAGGAAAGGTTCAATTTAGCATCCTGTTGACAGTGCTAAATAAC740.11734483524150835No Hit
ACTATGGGGAACTTCACAGGACGTGGAACATGTAAATGCATTTTGAGTTGAA740.11734483524150835No Hit
GTAAACGGGGATATGAAAACTGTTGGAGATACAAGAAAACAGACACAGAAAT730.11575909422473121No Hit
TGAAATGGGTTCGGGTGTTTTTTTCTATGTATTCATAGCAACAATAACACAA730.11575909422473121No Hit
TTCTGTGGGGATGGGGATGGTTCTTCTAAATGAAGCAGCAACTTCGAAAGGC720.11417335320795408No Hit
ATTACCGGGAGTGTTGTGTCCCTTTCTGATTTTCAAATACATCACACCTGCC720.11417335320795408No Hit
GCGCTGGGGTGAGCACGGGATATTAACTCTTTTGGAAACATGAACTCTTACT710.11258761219117694No Hit
TACACGGGGGACATACACACATTGAATTTGTGTACCTTACAGTACACAATAT710.11258761219117694No Hit
ATCCCAGGGGCGGTCATGCAAGTCTTCACTGAGTGGCTGCGGGACGGCCACG710.11258761219117694No Hit
GAAATTGGGAGTTGAAATTCCCAAGAACAGGAAAAAGATGATGGCTCAGAAA700.11100187117439979No Hit
AAAATGGGGGTTCTTCCTCCTTCCTCCACTAACCTCCAGTTCCTGAGAAGGC700.11100187117439979No Hit
TATCTAGGGAACTCGTGTAGTTGTCTGGTTTCAGGGATAGCTGTTTGAATTG700.11100187117439979No Hit
AATGCCGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA700.11100187117439979No Hit
AAACAAGGGATGCTACCATGTTGGATAAAGAAGCCAAACCATACAAAGTTGA690.10941613015762267No Hit
TACAAGGGAATAATTTAAAACTACTATAGAAACTGCAGAGCAAAGGAAGTGG690.10941613015762267No Hit
CTTTGTGGGATTCTAATTACATTTAAAATGTATGGATAGGACAAAAAAAGAA690.10941613015762267No Hit
TTTCCAGGGATGCACACTTCCCAGCTTGAACTTGAACATTTGTTTCTGTCTT690.10941613015762267No Hit
ACCGCAGGGGGCAGACTTCGGTTGAGGAAACGGGTATTTCATGTCTCAGGGA690.10941613015762267No Hit
ATTGCAGGGTTGATAGTGGAATTATATATTTTTACTCTATGTTTCTCTACAT680.10783038914084551No Hit
CACTAAGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA680.10783038914084551No Hit
TCTCACGGGCAGAACTGCATCAGGATATGGTGGTACCAGGAGACGATAAAGT680.10783038914084551No Hit
TTCCACGGGATAATTACTTTTTTAAAGTTTCATGTACCTAAAGCATATGCTA680.10783038914084551No Hit
GTACAAGGGGTGGCCTCATGTGGCCTTGCCTCTGTGTGTGGCACACCTGATG680.10783038914084551No Hit
AGTCAGGGGGATTAACATTTTTTACAAATTTGCCTGCATAAAGATTAAATTG680.10783038914084551No Hit
CCATGCAGGGAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAA680.10783038914084551No Hit
CTTGAGCAGGGAGCTATTCGGTATGTTCAAACTCAGACACGAAAACCAGAAC680.10783038914084551No Hit
GTGCCAGGGGTTGAAGTGGAAAATCTAAGAGGAACTGAAGAGAAAGTCATGA670.10624464812406836No Hit
TTATTTGGGATGATTTTTTAGTGAGTCACTTTGGGAAGGTGATGATCAAGAA670.10624464812406836No Hit
GGGACTGGGGTGAGGCTGAGGTAGGAAGATTATTTGAACCCAGGAGTTTGAG670.10624464812406836No Hit
ACTATAGGGAGAAAAACCCAAACTGAGACTCTTAAGTTTTGTTTAGCAATGT660.10465890710729124No Hit
GTAAGCGGGCCAGGAATGAGCTTGGCCTCCAACATCCCTTGCCTGCTGCCAT660.10465890710729124No Hit
CTTCGAGGGATACCTTGATGTGTAACCAGATAATTTATCCTTTTAAAGGGGT660.10465890710729124No Hit
TGCTATGGGACCTTCACAGCAACAGCTGACTGGGATTTGACGGAACAACTGA660.10465890710729124No Hit
GCCAATGGGGGGATTACAGGCATGAGCCACCACGCCCGGCTGACTTTTTTTC650.10307316609051409No Hit
GCAATGGGGAATAGAAAGTCAGATGACTACCGTGTTGGTTGTGACTTCCCCT650.10307316609051409No Hit
AGCGTAGGGAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAA650.10307316609051409No Hit
ATTTAAGGGCATGTGGTAGAAAACTTAACCAAAATGGGCTTTAAAAATTAAA650.10307316609051409No Hit
TACGCTGGGGAAGGTACTTCAGCTTAATCTGAAATATAATTTAACTTGTGAA650.10307316609051409No Hit
TACCTAGGGGTTGGGAGAATTTAAATATTGGTAATACTTCTTTGAATGTTTA650.10307316609051409No Hit
CCCATGGGGATAGAGTGACTGGCATGGGGAATGGGTGGTAGAGGTCAAATGA650.10307316609051409No Hit
TGTGGCGGGGAAAGGAAGTTTGGGCTAGACCATGTGGCGACCACAGACCAGG650.10307316609051409No Hit
CGCTTTGGGATTTAAAAACTTATTATAGAAAACTGAGGGCATATAAAAAACT640.10148742507373695No Hit
GAGAGAGGGACTTGCTTTGGTGATTGCAATCAGCATGGGATTTGGCCATTTC640.10148742507373695No Hit
TAGAATGGGATTTCTAAGTGGGTAAAAAATACTCTTACAGAATTAAGTGAAT640.10148742507373695No Hit
AATACTGGGACTAATCTTTAACGTGAAGGTTTTTGAAGGTGGTTCATTCTAC640.10148742507373695No Hit
TGAACGGGGACTGTGCCTGAGCTCTGGAGTGCCAAGGCCTACCCATCGCTCA640.10148742507373695No Hit
TGTGCTGGGGAGACATTCTGGTGATGATTTAGGGGCTTTTTATACACCACAT640.10148742507373695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)