FastQCFastQC Report
Sat 31 Dec 2022
SRR5849683.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849683.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75038
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC7020.935526000159919No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT5860.7809376582531518No Hit
CGTCAGGGGATGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCCAG1170.15592100002665316No Hit
GAGCTTGGGCGAGATGGGGCTAACCTTTATTAAACAGATCGCATATTATGAT1130.15059036754710947No Hit
TAAGCTGGGGCCCCCAGGTGAGATTTCCATCCATTTCATGATAAATTCCAAC1120.14925770942722352No Hit
ACTCGTGGGTATTCAAGACCAAGGTAGCAGAAAAGTTACTTGTATCTGCTTA1010.13459847010847836No Hit
ATATGCGGCATATTACCTGACTTCCAATTATTCAACAGAGCTACAGTAACCA1010.13459847010847836No Hit
TTCGACGGGAGGCTAAATTTGATGCAAACCAGCAAGATGGGAAATGAGACAT1010.13459847010847836No Hit
ATGCATCGGGGATCTTGTAGCGGGGCAGGAACAGGAGAATGACATCTTCCTG990.13193315386870652No Hit
TATCAAGGGATCGTTGAGACTTCGGAACCTCAAGAACAAACTGGAGAATAAA950.1266025213891628No Hit
AGCTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA950.1266025213891628No Hit
GCCGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA940.12526986326927692No Hit
TCTAATGGGATAATAAAAATAGCAGATGTTGATTAAGAAATTGGGCAGGCCA940.12526986326927692No Hit
GGAGCTGGGGAAGGAGAAGGCTTCATCCTTGGGGGAGTTTTCGTGGTGGGAT930.12393720514939097No Hit
TTGCTAGGGGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG930.12393720514939097No Hit
GCCTATGGGCAGGATTCAAGTTGGAGTTCTACAATGAGATTAGTTTTCCTCA920.12260454702950506No Hit
AATGCAGGGCCGTTCAATTGCATAGAGCATGAACAATTTCTGACCAGATTTT910.12127188890961912No Hit
GCTGCCGGATTTTAAACTTTTGACGGTTTGAAACTGTAAATTTTGAAACCAT900.1199392307897332No Hit
GCGACTGGGCAAAGTGGAAACTAAATCCCCTACATGCTCATAGGACTTTTTT890.11860657266984727No Hit
GGATCAGGGGCAGCAAGACAATTTAAAAAGAGGATAATTAAAAACTTTTAAA880.11727391454996136No Hit
ACATAAGGGACTTCTGGTCTTTTTGGCCGGGAACTCCTGATTGGTGTTAAGG880.11727391454996136No Hit
AGATTCGGGAATGAACGCCAACTTTCATCTTTGAAGGGTTAGCTTCTATTAC880.11727391454996136No Hit
CCATACGGGGCCTGGGTGGGGCTGGGCCCAGGAAGGGGTGGGCTGGATTTTG880.11727391454996136No Hit
GGCCATGGGCAAACTGATGCAGCTATTGATTTTGGAAACTCTGGAGGTCCCC870.11594125643007543No Hit
TATCCTGGGATTATTTGTTATTACCTCTTTAAATTTTCATTTTATACAATTT870.11594125643007543No Hit
TAATATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA870.11594125643007543No Hit
TAGTTCGGGATGAGCAGGCTGAGAGTGACATCATGAAGAGACAGCCCAGAAA870.11594125643007543No Hit
ATTTCAGGGAGACAGACCTGTTTCTAAAGCCTGGTTTTTCTGGTTTCTAGCA870.11594125643007543No Hit
TGCTGTGGGAGACCTAAGTATTTGCCTTTATGAAGCCAATGCACTGTTGGGA860.1146085983101895No Hit
TTATGAGGGGTAGATGTAATTCAATTTGACTAGTGTTTATTAAGTTCCTAAT860.1146085983101895No Hit
TTTTACGGGAGATGAATAATTTATAGATGTGCATCCATTTTATTTTTATAAG860.1146085983101895No Hit
TGTCGAGGGGATCTCTTTCGGGGTCAAGCCAGAAGCCAGTCTCAAAAAAATT860.1146085983101895No Hit
TGCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA860.1146085983101895No Hit
GGATTAGGGATTGGTCTTTCATTAATTTTGCTTGATATTTGATGTGCCCTTA860.1146085983101895No Hit
CACGTTGGGTGAACCAAAAGACTTTACATTCTAGCATTAGGAACACACACAA860.1146085983101895No Hit
TGCTCCGGGGTGTGTTTTCTGTCTTAGAAAAGAATGTGTTCTAATGCAAGGA850.11327594019030357No Hit
ACCAGGGGGAATGCCAGTCCTTCTCAAACTCTTCCAAAAATTTGAAGAGGAG850.11327594019030357No Hit
GTTGCAGGGATTTGACTTTCTATGATGTGCGGGGCCTCTACTTGATTATGTA850.11327594019030357No Hit
TTCTCAGGGACTGCCACGGTCCTTTGAGTGTCAGGCTCTGAATCTCTCAAAA850.11327594019030357No Hit
AACAGGGTAAGGGATTAATAGCTAGAATACACAAGAAACTCAAATAACTCAA850.11327594019030357No Hit
CCGACCGGGTTCGTTGACTCACTTTTTACTTTCCTCCTTGAGTTTACCAAAC850.11327594019030357No Hit
TTTGCGGGGGCTGTGGTGTTGGGGTGGGCATGGAGGGGCAGCGGCAGGCTGC840.11194328207041765No Hit
ATGTGCGGGCCCAAAGCAGTGCCAGCCGTCCGACACCGTGTGTGCCAGTGTC830.11061062395053171No Hit
TATAATGGGGTCAGATGTTTCCTATCAATGGATCCAGATTCAAATGCAGAAA830.11061062395053171No Hit
GAACAGGGGGAGTCCTCAGCGGGGCGCGTGGCGAGCGGACTCGACTCGGCAC830.11061062395053171No Hit
TGTGATGGGATAGAAAGCATAATGAAAATGTCCTGTGACTGAAGTATGTGTA830.11061062395053171No Hit
CTCAAAGGGTGTTTTTTATGGACACCATGCAAAAATCGTCTGCCTGGCCTGG830.11061062395053171No Hit
GCCAAAGGGGAAGAATGCATTTCACAATAAAGTTATTTTTTACTAGTGTTTT830.11061062395053171No Hit
TGCAATGGGCCTCTCCTTTGATTCTCAATCTTCCCCCAAAGCATGGTTTGGT820.1092779658306458No Hit
CGCCTGGGGCCACATCTCAGTCAACTTACTGGCAGTAAAATGAAGCTACTGA820.1092779658306458No Hit
ATATTGGGGATCCGTAAGATGAAGGTGACAACAAAAGTCAACCGTCATGACA820.1092779658306458No Hit
GTCAGAGGGGTGCCTAATATTTTATGTTCTGTTTTTGTCATTTGGCGTGATT810.10794530771075989No Hit
TATCTGGGGGAGGCAGCCGGGAGGAGCGGCGCGCGCTCGGACCTCTCCCGCC810.10794530771075989No Hit
GTTACTGGGAAGATAGCCAGCAATGCTTAGAAGACTTTTTTTCTTAAAGCAG810.10794530771075989No Hit
TCATTAGGGAGCATAAAAAATATTAACAGAAACAAAAATAAATGAATGAATG810.10794530771075989No Hit
CAGTCCTAGATTTTTACCTACGAGAAATAAGACGACACAAAAACCCATTCCC810.10794530771075989No Hit
ATACATGGGCAATATGCCTTCCTCACTAAGCTCAATCATTTCTAGCTTTTGA800.10661264959087395No Hit
CTTTGGCGGGGGGCCGTGGCCCGGCTGGGTCGGCTGAAGAACTGCGGATGGA800.10661264959087395No Hit
CCAGCCTGGGTGACTGAGCGAGACTCCGTCTCAAAAAAAATAAAGAAGTAAA800.10661264959087395No Hit
ATCCTAGGGAGGCAGTCTTGTCTACAGTTTCTGCATACATATTTCACATATA800.10661264959087395No Hit
CTGTACGGGGCTCTTCCGTTCTTGCAGTGGGGAGTAGGGCCGAGCGCGGAAC800.10661264959087395No Hit
ATACTAGGGTACTATGACAAGCGCTGGTCCTGTGAACTCTTCCTGCTGGTGT790.10527999147098804No Hit
TGCAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA790.10527999147098804No Hit
GAACAGGGGCCCAAATTGAAAAACTTGTGCGCTACTGGTGTTATGGAAAATC790.10527999147098804No Hit
GTATAAGGGAACAAGTGATGTTGATAAAAGCCAGAGGAACAAACATGGGTGA790.10527999147098804No Hit
GTTGCTGGGACTGTGTCGTCTGAAGGCCTGGCTGGGCTGAAGCATCTGCTTC790.10527999147098804No Hit
GCCCTCATGCCAGCAGGAGTCATGCCAGAGATCATATCTGAGAAAGCAAGAC790.10527999147098804No Hit
AACTCAGGGAGGGTGAAATTTAGCCATCGGTGTGTGGCAGGGTCATGAAAAA780.10394733335110211No Hit
GGATATGGGGGGGATGTCATCCTGACTGGGACCCCCCCACCTCCACCCAGGT780.10394733335110211No Hit
TTTTCTAGGGGGGGCTTTTAACGGCCATCGGCCCCTCCCAGCCTGGACAAAG780.10394733335110211No Hit
TCGTTTGGGACTAGATAACGTATTTTTCTACCTGATACTCCTCAAATAAATT780.10394733335110211No Hit
GTAATGGGGTAGCAGCGGGGTGCTTCAGCCTATCTAGATTCAGGGCTTGAAA780.10394733335110211No Hit
TGACAGGGGTAACTTGGCTGTCTTTAAGTAAATTACTTGTAGTCAAAAGTTT780.10394733335110211No Hit
TTACAGGGAGAGACTTTGAAGGTCTGGGTCAAAAATGCCAAGTCCAGCCCCC780.10394733335110211No Hit
TACAGGGACATAAAATATATTTATAGTACTCTAATTTATGTTGTAAAGTATG780.10394733335110211No Hit
CACAGTGGGATGACAGTTTTATAAATAAATCACTGAAAGTTACACAGCCAGC770.10261467523121619No Hit
TAGTAGGGGCATTTGGAAGAACTGCAGATTTTCCTCCTTCAAAATTAAAGTC770.10261467523121619No Hit
ATGCAGGCGCGAATTTGGATTGTGTGATTTATTTTTTGAAGTTTTATTGGAG770.10261467523121619No Hit
AGAATAGGGAAATGTGTCAAATTTGTGATTTGTTTTGGGTGACCTCAATTAT770.10261467523121619No Hit
AAAGAGGGAGAAATGACCACCTCATGGACAATGTTATTTTATTTAGATGCCC770.10261467523121619No Hit
AGTGGTGGGACCCCCTTTAGCGGGACTCAGTGAGCTGGGGCCGTCTGTGTGG770.10261467523121619No Hit
TCCAGTGGGAACATGAAAACAGGCTAGTTTCAAGCAACAGCAATGTCGGTTG770.10261467523121619No Hit
GTAATAGGGAAATGGGCTGGCAGGAAGACAGTGAAAATGATGAAACATGTGC770.10261467523121619No Hit
TTTGGCGGGACCACCCAAATTCAGAACATGATTGGGGAAATGAAAATAGCCC770.10261467523121619No Hit
TACTCAGGGCGATCTTTTTCGGGAAGCTTTTACTGTGCTTTTTACTGAACAA770.10261467523121619No Hit
CGTAGTGGTCCACCACCAGATAAGATTTAAGGGTAGCAATTGTAATAAATCA760.10128201711133025No Hit
AGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGA760.10128201711133025No Hit
TAAGCAGGGGAAGCCGGCAAGATGGCGGCGGCTGGGGCTGGCCGTCTGAGGC760.10128201711133025No Hit
TGCAGGGACAGCGGGTGAGCTGGGCAGGTGTGGAGAATGTCTCTGGCTGGCG760.10128201711133025No Hit
GTAAAAGGGCACTGCCGCTGTGCTTTCGTGGAAATGACAGTTCCTTGTTTTT760.10128201711133025No Hit
ACAGCTGGTTTATTGCTATATCCACTTCCGAGGCACTCTTTCATTGCCCTGT760.10128201711133025No Hit
GCGTAAGGGCTCCTACTCAGACCCATCACATTTCTTCACAGGTTTGGTTCAC760.10128201711133025No Hit
TCGCATGGGGGTTATTTAGAGTACCTATTTCTTCCTGAGTAAAATTTGGTTG760.10128201711133025No Hit
CGTCAGGGGGTGAACCTACTTTTGGAAAGCAAGCTGGGTATCTGAAATCCTA760.10128201711133025No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)