FastQCFastQC Report
Sat 31 Dec 2022
SRR5849682.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849682.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75050
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC6570.8754163890739507No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT6330.843437708194537No Hit
ACTCGTGGGTATTCAAGACCAAGGTAGCAGAAAAGTTACTTGTATCTGCTTA1150.15323117921385743No Hit
CGTCAGGGGATGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCCAG1130.15056628914057293No Hit
AGCTAAGGGATTTCTTGGAAGCATCCCTGGCAAAAATGCAGCTGAGTACAAG1040.13857428381079281No Hit
AGTGGTGGGACCCCCTTTAGCGGGACTCAGTGAGCTGGGGCCGTCTGTGTGG1030.13724183877415055No Hit
CCAGCCTGGGTGACTGAGCGAGACTCCGTCTCAAAAAAAATAAAGAAGTAAA1020.13590939373750832No Hit
ATGCATCGGGGATCTTGTAGCGGGGCAGGAACAGGAGAATGACATCTTCCTG1010.1345769487008661No Hit
TGCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1010.1345769487008661No Hit
TTCGACGGGAGGCTAAATTTGATGCAAACCAGCAAGATGGGAAATGAGACAT1010.1345769487008661No Hit
GGAGCTGGGGAAGGAGAAGGCTTCATCCTTGGGGGAGTTTTCGTGGTGGGAT1000.13324450366422386No Hit
TATCTGGGGGAGGCAGCCGGGAGGAGCGGCGCGCGCTCGGACCTCTCCCGCC990.1319120586275816No Hit
CACGTGGGGCTCAGTAGGCAATATAGTGCAGAGATTGAGAGCACAGACCACT990.1319120586275816No Hit
GCTGCCGGATTTTAAACTTTTGACGGTTTGAAACTGTAAATTTTGAAACCAT980.13057961359093936No Hit
GAACAGGGGGAGTCCTCAGCGGGGCGCGTGGCGAGCGGACTCGACTCGGCAC970.12924716855429713No Hit
ACCAGGGGGAATGCCAGTCCTTCTCAAACTCTTCCAAAAATTTGAAGAGGAG970.12924716855429713No Hit
TCGTTTGGGACTAGATAACGTATTTTTCTACCTGATACTCCTCAAATAAATT970.12924716855429713No Hit
TCTAATGGGATAATAAAAATAGCAGATGTTGATTAAGAAATTGGGCAGGCCA960.1279147235176549No Hit
CACGTTGGGTGAACCAAAAGACTTTACATTCTAGCATTAGGAACACACACAA960.1279147235176549No Hit
AGAATAGGGAAATGTGTCAAATTTGTGATTTGTTTTGGGTGACCTCAATTAT950.12658227848101267No Hit
TTATAAGGGATATCATGGGAAATTCAAAGGAGGCAAAAGTTCAAAGCATTGC950.12658227848101267No Hit
GGTGCTGGGGTGGGCGGGAGGTGGTTATGGCTGAAGGAGTGGAGCCCCATCC950.12658227848101267No Hit
CGTCAGGGGGTGAACCTACTTTTGGAAAGCAAGCTGGGTATCTGAAATCCTA940.1252498334443704No Hit
TTATGAGGGGTAGATGTAATTCAATTTGACTAGTGTTTATTAAGTTCCTAAT930.12391738840772817No Hit
TGCAGGGACAGCGGGTGAGCTGGGCAGGTGTGGAGAATGTCTCTGGCTGGCG930.12391738840772817No Hit
ATATGCGGCATATTACCTGACTTCCAATTATTCAACAGAGCTACAGTAACCA930.12391738840772817No Hit
TACAGGGACATAAAATATATTTATAGTACTCTAATTTATGTTGTAAAGTATG910.12125249833444371No Hit
GTTCACGGGACAGAAGGGAAATGAGCTTTTTCACTGTAATAGTTCAGTGGGT900.11992005329780146No Hit
AGATTCGGGAATGAACGCCAACTTTCATCTTTGAAGGGTTAGCTTCTATTAC900.11992005329780146No Hit
TTGGGGTGGGGAATCATAAAAGATTGGGATTTAAAAATAATTTCTTAGCAAT900.11992005329780146No Hit
GATGGTGGGATGATATCAATTGTAATGTTTCTATTTTCATTTCTGATAGTGT890.11858760826115923No Hit
CTTGGGGGGAGTCCTGCTGCAGTTGTGCAGCAGAGGAGACTGCAGACTTCGG890.11858760826115923No Hit
TGCGCACGGGACTTACTCAACATAAAAGAATTCATACTGGTAAGAAATCTTA890.11858760826115923No Hit
CACAGTGGGATGACAGTTTTATAAATAAATCACTGAAAGTTACACAGCCAGC880.11725516322451698No Hit
TCCAGTGGGAACATGAAAACAGGCTAGTTTCAAGCAACAGCAATGTCGGTTG880.11725516322451698No Hit
CGTAGTGGTCCACCACCAGATAAGATTTAAGGGTAGCAATTGTAATAAATCA870.11592271818787477No Hit
GCCGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA870.11592271818787477No Hit
TTTGCGGGGGCTGTGGTGTTGGGGTGGGCATGGAGGGGCAGCGGCAGGCTGC870.11592271818787477No Hit
GGAACCGGGTATGACATTCTACATGTTAGGTAGAATATATATGGGCATACTT870.11592271818787477No Hit
TCGCATGGGGGTTATTTAGAGTACCTATTTCTTCCTGAGTAAAATTTGGTTG870.11592271818787477No Hit
ATACTAGGGTACTATGACAAGCGCTGGTCCTGTGAACTCTTCCTGCTGGTGT860.11459027315123252No Hit
TAAGCTGGGGCCCCCAGGTGAGATTTCCATCCATTTCATGATAAATTCCAAC860.11459027315123252No Hit
CCATACGGGGCCTGGGTGGGGCTGGGCCCAGGAAGGGGTGGGCTGGATTTTG860.11459027315123252No Hit
TTGCTAGGGGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG860.11459027315123252No Hit
AAAACTGGGATCTGTATTTCTACGTCTACCTGAGTATAGAAAAATACACTGT850.11325782811459027No Hit
TCATTAGGGAGCATAAAAAATATTAACAGAAACAAAAATAAATGAATGAATG850.11325782811459027No Hit
TTTTAGGGGGGAGGAAGAGAGGTTTTGACTCACACTGAAGCTCACTCCGTTG850.11325782811459027No Hit
ATATTGGGGATCCGTAAGATGAAGGTGACAACAAAAGTCAACCGTCATGACA850.11325782811459027No Hit
GTCTGGGGGCCGTGATTGCAGGAGCCCTGGCCGTGGAATTGATGGTATCTGT850.11325782811459027No Hit
GCTGTGGGGGCTCTTCCGATCTATGTGTGGGGGAAGCCCAAGAGGAATAGTA840.11192538307794803No Hit
GTTACTGGGAAGATAGCCAGCAATGCTTAGAAGACTTTTTTTCTTAAAGCAG840.11192538307794803No Hit
GCGACTGGGCAAAGTGGAAACTAAATCCCCTACATGCTCATAGGACTTTTTT840.11192538307794803No Hit
GAGCTTGGGCGAGATGGGGCTAACCTTTATTAAACAGATCGCATATTATGAT840.11192538307794803No Hit
AGTACTGGGGGGAAACAGCTTTACTACTTTTTTTATTGCGTCTGGCCTCACT830.1105929380413058No Hit
GGATATGGGGGGGATGTCATCCTGACTGGGACCCCCCCACCTCCACCCAGGT830.1105929380413058No Hit
AAAGAGGGAGAAATGACCACCTCATGGACAATGTTATTTTATTTAGATGCCC830.1105929380413058No Hit
CAATAAGGGGGGCCAGATGGCCAGCTGTCGAGAGCAGCCCTGCCCAGATTGC830.1105929380413058No Hit
GGATTAGGGATTGGTCTTTCATTAATTTTGCTTGATATTTGATGTGCCCTTA830.1105929380413058No Hit
CTGTACGGGGCTCTTCCGTTCTTGCAGTGGGGAGTAGGGCCGAGCGCGGAAC830.1105929380413058No Hit
AATGCAGGGCCGTTCAATTGCATAGAGCATGAACAATTTCTGACCAGATTTT820.10926049300466356No Hit
AGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGA820.10926049300466356No Hit
CAAGCAGGGGCAGATTTGGAGAAAATGAAAGTGGAACATGATGTCTGGAGGC820.10926049300466356No Hit
GTAGATGGGACTTGAATGCCAAATTAATTTTGTTTTAAGAAAGCTTATTTTT820.10926049300466356No Hit
GCTTCCGGGGCAGATTTGGCAGAGAGTATAATGAAGAATCTTAGGCGGGTGC820.10926049300466356No Hit
GTTGCAGGGATTTGACTTTCTATGATGTGCGGGGCCTCTACTTGATTATGTA820.10926049300466356No Hit
TAGTTCGGGATGAGCAGGCTGAGAGTGACATCATGAAGAGACAGCCCAGAAA820.10926049300466356No Hit
ATGCAGGCGCGAATTTGGATTGTGTGATTTATTTTTTGAAGTTTTATTGGAG810.10792804796802133No Hit
CTTTGGCGGGGGGCCGTGGCCCGGCTGGGTCGGCTGAAGAACTGCGGATGGA810.10792804796802133No Hit
GGCCATGGGCAAACTGATGCAGCTATTGATTTTGGAAACTCTGGAGGTCCCC810.10792804796802133No Hit
CTATTGGGGGGTTATTTATTTTGCCTGTCCTTATCCCTGCTTGGACACCTGA810.10792804796802133No Hit
GCCAAAGGGGAAGAATGCATTTCACAATAAAGTTATTTTTTACTAGTGTTTT810.10792804796802133No Hit
GTACTTGGGTCTCGGTGCGCGGCAGCCTCTTGCTGTAGTTCTTCTTTTCTGG800.10659560293137908No Hit
TTCCAGGGGGAGCCCCCCACTTTGGGTGGGAGAGGCGAGGGAAAGCCCAAAG800.10659560293137908No Hit
GTTGCAGGGATTGACGAAGAAGGGGATGAGGATGAAGGTGAAGAAGATGAAG800.10659560293137908No Hit
CACCTGGGAGTTAATATTGTGTATTAAAGGATAAGTCTTAATGCTCAAAGTA800.10659560293137908No Hit
GTATATGGGTGGAATTTACTGTATGTTGCAGTAACTCGAGCCAAGAAGCGTC800.10659560293137908No Hit
TGACAGGGGTAACTTGGCTGTCTTTAAGTAAATTACTTGTAGTCAAAAGTTT800.10659560293137908No Hit
ATTTACGGGGTATATTCAAATCCTACAATATATTCAATTTTTTCCAATTATC790.10526315789473684No Hit
ACGTTGGGGATATTCTTTCTCCTTCTCCTCTGCTCTCTGGTCCAGAATGAAG790.10526315789473684No Hit
TCGCTGGGGGATCTAAAAACGGGAGGGTCCACTTTGGTAATGTGTCAAAGAG790.10526315789473684No Hit
TTCTCAGGGACTGCCACGGTCCTTTGAGTGTCAGGCTCTGAATCTCTCAAAA790.10526315789473684No Hit
TCATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA790.10526315789473684No Hit
ACTAGCGGGTTACAGATAACCTAAATGTTTATAAAAATAGATTGTCAACAGG780.1039307128580946No Hit
CAAGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC780.1039307128580946No Hit
GTAAAAGGGCACTGCCGCTGTGCTTTCGTGGAAATGACAGTTCCTTGTTTTT780.1039307128580946No Hit
GCCTATGGGCAGGATTCAAGTTGGAGTTCTACAATGAGATTAGTTTTCCTCA780.1039307128580946No Hit
TATCCTGGGATTATTTGTTATTACCTCTTTAAATTTTCATTTTATACAATTT770.10259826782145237No Hit
AGACATGGGATGAAGCAGTGGACTATGTTATTCGCCACTTGATTCAAGAAAA770.10259826782145237No Hit
CAAATAGGGAAATAGTGTACTCTTCTGTCATCTCTATTTGACAAACATACTT770.10259826782145237No Hit
GTCAGAGGGGTGCCTAATATTTTATGTTCTGTTTTTGTCATTTGGCGTGATT760.10126582278481014No Hit
CATAGCGGGCACGTTCTGTCATTTTCCACTTTAGGAATTTCCAGTATTCCAA760.10126582278481014No Hit
ATAGCGGGTCCTGATCTTCCTCCTCAATTTTTTACAGTAGCACTAAATAAAC760.10126582278481014No Hit
CAACCAGGGAGGCGTAATTAAATGCCTTCTTGAGGACTTTTATACCTTCCTC760.10126582278481014No Hit
CGATGGGCGGGGGCTAAGCAGATTGTGTGGAATGGTCCTGTGGGGGTATTTG760.10126582278481014No Hit
CTACTAGGGCTTCTAATTTGTTAATAGAGAGAATTTATTAGAAATGCATTTC760.10126582278481014No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATG760.10126582278481014No Hit
TAAGCAGGGGAAGCCGGCAAGATGGCGGCGGCTGGGGCTGGCCGTCTGAGGC760.10126582278481014No Hit
TATTAAGGGGAGGTTGCGGGTCGGAGGGCAGAAGCACAGTTCCCAGTACAGA760.10126582278481014No Hit
ACTGGTGGGGAACCTGGGAGGTGGAGGTTGCAGTGAGCTGAGATTGCACCAC760.10126582278481014No Hit
TTGCAGGGTCCTTTACTGAATTGTCAGTGATACTTATTTTCAGAGACATTAG760.10126582278481014No Hit
GCCCTCATGCCAGCAGGAGTCATGCCAGAGATCATATCTGAGAAAGCAAGAC760.10126582278481014No Hit
ATTTCAGGGAGACAGACCTGTTTCTAAAGCCTGGTTTTTCTGGTTTCTAGCA760.10126582278481014No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)