FastQCFastQC Report
Sat 31 Dec 2022
SRR5849681.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849681.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73557
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC6120.8320078306619356No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT6080.8265698709844067No Hit
AGATTCGGGAATGAACGCCAACTTTCATCTTTGAAGGGTTAGCTTCTATTAC1030.1400274616963715No Hit
GCGACTGGGCAAAGTGGAAACTAAATCCCCTACATGCTCATAGGACTTTTTT1020.13866797177698928No Hit
GCCCTCATGCCAGCAGGAGTCATGCCAGAGATCATATCTGAGAAAGCAAGAC1010.13730848185760702No Hit
GAGCTTGGGCGAGATGGGGCTAACCTTTATTAAACAGATCGCATATTATGAT990.13458950201884254No Hit
AGTGGTGGGACCCCCTTTAGCGGGACTCAGTGAGCTGGGGCCGTCTGTGTGG980.13323001209946028No Hit
TTTGCGGGGGCTGTGGTGTTGGGGTGGGCATGGAGGGGCAGCGGCAGGCTGC980.13323001209946028No Hit
CACGTTGGGTGAACCAAAAGACTTTACATTCTAGCATTAGGAACACACACAA980.13323001209946028No Hit
ACCAGGGGGAATGCCAGTCCTTCTCAAACTCTTCCAAAAATTTGAAGAGGAG960.13051103226069577No Hit
TCGCATGGGGGTTATTTAGAGTACCTATTTCTTCCTGAGTAAAATTTGGTTG950.12915154234131354No Hit
AATGCAGGGCCGTTCAATTGCATAGAGCATGAACAATTTCTGACCAGATTTT930.12643256250254906No Hit
TAAGCTGGGGCCCCCAGGTGAGATTTCCATCCATTTCATGATAAATTCCAAC930.12643256250254906No Hit
TTATAAGGGATATCATGGGAAATTCAAAGGAGGCAAAAGTTCAAAGCATTGC930.12643256250254906No Hit
GTTGCAGGGATTGACGAAGAAGGGGATGAGGATGAAGGTGAAGAAGATGAAG920.1250730725831668No Hit
GCCGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA920.1250730725831668No Hit
TCTAATGGGATAATAAAAATAGCAGATGTTGATTAAGAAATTGGGCAGGCCA920.1250730725831668No Hit
CGTCAGGGGGTGAACCTACTTTTGGAAAGCAAGCTGGGTATCTGAAATCCTA920.1250730725831668No Hit
CGTCAGGGGATGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCCAG910.12371358266378456No Hit
ACTCGTGGGTATTCAAGACCAAGGTAGCAGAAAAGTTACTTGTATCTGCTTA900.1223540927444023No Hit
CATAGCGGGCACGTTCTGTCATTTTCCACTTTAGGAATTTCCAGTATTCCAA890.12099460282502005No Hit
ATGCATCGGGGATCTTGTAGCGGGGCAGGAACAGGAGAATGACATCTTCCTG890.12099460282502005No Hit
GTTCACGGGACAGAAGGGAAATGAGCTTTTTCACTGTAATAGTTCAGTGGGT880.1196351129056378No Hit
ATATGCGGCATATTACCTGACTTCCAATTATTCAACAGAGCTACAGTAACCA880.1196351129056378No Hit
GCTGTGGGGGCTCTTCCGATCTATGTGTGGGGGAAGCCCAAGAGGAATAGTA870.11827562298625555No Hit
TATCCTGGGATTATTTGTTATTACCTCTTTAAATTTTCATTTTATACAATTT870.11827562298625555No Hit
TGCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA870.11827562298625555No Hit
ACAGCTGGTTTATTGCTATATCCACTTCCGAGGCACTCTTTCATTGCCCTGT860.1169161330668733No Hit
ATGTGCGGGCCCAAAGCAGTGCCAGCCGTCCGACACCGTGTGTGCCAGTGTC850.11555664314749108No Hit
GCGTAAGGGCTCCTACTCAGACCCATCACATTTCTTCACAGGTTTGGTTCAC850.11555664314749108No Hit
TTGGGGTGGGGAATCATAAAAGATTGGGATTTAAAAATAATTTCTTAGCAAT850.11555664314749108No Hit
GTTGCAGGGATTTGACTTTCTATGATGTGCGGGGCCTCTACTTGATTATGTA850.11555664314749108No Hit
GGATATGGGGGGGATGTCATCCTGACTGGGACCCCCCCACCTCCACCCAGGT840.11419715322810882No Hit
TTATGAGGGGTAGATGTAATTCAATTTGACTAGTGTTTATTAAGTTCCTAAT830.11283766330872658No Hit
ATACTAGGGTACTATGACAAGCGCTGGTCCTGTGAACTCTTCCTGCTGGTGT830.11283766330872658No Hit
TTTTAGGGGGGAGGAAGAGAGGTTTTGACTCACACTGAAGCTCACTCCGTTG830.11283766330872658No Hit
GGATTAGGGATTGGTCTTTCATTAATTTTGCTTGATATTTGATGTGCCCTTA830.11283766330872658No Hit
GCATGGGGAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT820.11147817338934432No Hit
TCGCTGGGGGATCTAAAAACGGGAGGGTCCACTTTGGTAATGTGTCAAAGAG820.11147817338934432No Hit
TAATATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA820.11147817338934432No Hit
CAGTCCTAGATTTTTACCTACGAGAAATAAGACGACACAAAAACCCATTCCC820.11147817338934432No Hit
TCAAGAGGGGATTTTTAAAGTGGTATTCACATTTTAACAAAAGAGCAATGAA810.11011868346996206No Hit
GGCCATGGGCAAACTGATGCAGCTATTGATTTTGGAAACTCTGGAGGTCCCC810.11011868346996206No Hit
TCGTTTGGGACTAGATAACGTATTTTTCTACCTGATACTCCTCAAATAAATT810.11011868346996206No Hit
TTTGGCGGGACCACCCAAATTCAGAACATGATTGGGGAAATGAAAATAGCCC810.11011868346996206No Hit
GTTGCTGGGACTGTGTCGTCTGAAGGCCTGGCTGGGCTGAAGCATCTGCTTC800.10875919355057982No Hit
CCATACGGGGCCTGGGTGGGGCTGGGCCCAGGAAGGGGTGGGCTGGATTTTG800.10875919355057982No Hit
AGCGGCGGGGTATGCTCAAAGTCACAAAACCTTAGTTTCCTTATGTGCATGA800.10875919355057982No Hit
GCCAAAGGGGAAGAATGCATTTCACAATAAAGTTATTTTTTACTAGTGTTTT800.10875919355057982No Hit
TGCGCACGGGACTTACTCAACATAAAAGAATTCATACTGGTAAGAAATCTTA800.10875919355057982No Hit
TTAAGGGGGACAGTTGTAATTATTTGTGAAGTCTGAAACTAATAACTATTTC790.10739970363119757No Hit
GGAGCTGGGGAAGGAGAAGGCTTCATCCTTGGGGGAGTTTTCGTGGTGGGAT790.10739970363119757No Hit
AAAGAGGGAGAAATGACCACCTCATGGACAATGTTATTTTATTTAGATGCCC790.10739970363119757No Hit
TGACAGGGGTAACTTGGCTGTCTTTAAGTAAATTACTTGTAGTCAAAAGTTT790.10739970363119757No Hit
TTCTCAGGGACTGCCACGGTCCTTTGAGTGTCAGGCTCTGAATCTCTCAAAA790.10739970363119757No Hit
TTGCTAGGGGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG790.10739970363119757No Hit
TGCTCGGGAGCCTTTTTTAGTGTGGAAAAAGTCTTTGATAAATTAAAAGTTT780.10604021371181532No Hit
TGATAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA780.10604021371181532No Hit
TTTTCTAGGGGGGGCTTTTAACGGCCATCGGCCCCTCCCAGCCTGGACAAAG780.10604021371181532No Hit
CCAACAGGGCGATCTGTCGTAGGGAAACATGACTATTACTAATGACTACCAA780.10604021371181532No Hit
AGACATGGGATGAAGCAGTGGACTATGTTATTCGCCACTTGATTCAAGAAAA780.10604021371181532No Hit
GATAAAGGGAACTCTGAGAGAAACATTAATTGATGAGGGTAATAATTTTGGT780.10604021371181532No Hit
AGCTAAGGGATTTCTTGGAAGCATCCCTGGCAAAAATGCAGCTGAGTACAAG770.10468072379243307No Hit
TTTTACGGGAGATGAATAATTTATAGATGTGCATCCATTTTATTTTTATAAG770.10468072379243307No Hit
TTAGCTGGGAAGTCACTAACCTTCCTCTTTGATCTCTTTGTAGGCTGACCAA770.10468072379243307No Hit
ACTAGCGGGTTACAGATAACCTAAATGTTTATAAAAATAGATTGTCAACAGG760.10332123387305084No Hit
ACACACGGGATGATGGTGCATAAATACCTCAACTGTCTGGGCTGAGAGAGGG760.10332123387305084No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATG760.10332123387305084No Hit
TTGCAGGGTCCTTTACTGAATTGTCAGTGATACTTATTTTCAGAGACATTAG760.10332123387305084No Hit
TTATCGGGACGAGACACAAAAATAATTACCTAATGATTTTATGAATTCAGCA760.10332123387305084No Hit
CAAGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC750.1019617439536686No Hit
TATCTGGGGGAGGCAGCCGGGAGGAGCGGCGCGCGCTCGGACCTCTCCCGCC750.1019617439536686No Hit
GACATAGGGAGCCATCCCTACAATTAGATGGGTCAAGCTAACACTCGTTCTT750.1019617439536686No Hit
GCTGCCGGATTTTAAACTTTTGACGGTTTGAAACTGTAAATTTTGAAACCAT750.1019617439536686No Hit
CAATAAGGGGGGCCAGATGGCCAGCTGTCGAGAGCAGCCCTGCCCAGATTGC750.1019617439536686No Hit
AAAGATGGGATAAAATGGCATGGTATTTTCATATAAATTATAAACATCCTCC750.1019617439536686No Hit
TGCTGTGGGAGACCTAAGTATTTGCCTTTATGAAGCCAATGCACTGTTGGGA740.10060225403428634No Hit
TTCCAGGGGGAGCCCCCCACTTTGGGTGGGAGAGGCGAGGGAAAGCCCAAAG740.10060225403428634No Hit
ATGTCAGGGGCCCTTTCTCAAGTCAGCCTGATAGATATGGCCAGGTTAGCAA740.10060225403428634No Hit
CTTGTAGGGGGCCCAGGACCCGGTGGCCGCGGCGCCCCGCCCCTTACCCGCC740.10060225403428634No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGAACTCGTATGC740.10060225403428634No Hit
ACATAAGGGACTTCTGGTCTTTTTGGCCGGGAACTCCTGATTGGTGTTAAGG740.10060225403428634No Hit
GTTACTGGGAAGATAGCCAGCAATGCTTAGAAGACTTTTTTTCTTAAAGCAG740.10060225403428634No Hit
GAACAGGGGGAGTCCTCAGCGGGGCGCGTGGCGAGCGGACTCGACTCGGCAC740.10060225403428634No Hit
TGTGATGGGATAGAAAGCATAATGAAAATGTCCTGTGACTGAAGTATGTGTA740.10060225403428634No Hit
CTATTGGGGGGTTATTTATTTTGCCTGTCCTTATCCCTGCTTGGACACCTGA740.10060225403428634No Hit
GTAAAAGGGCACTGCCGCTGTGCTTTCGTGGAAATGACAGTTCCTTGTTTTT740.10060225403428634No Hit
GCTTCCGGGGCAGATTTGGCAGAGAGTATAATGAAGAATCTTAGGCGGGTGC740.10060225403428634No Hit
CATATTGGGCAAGGTCCCAAAGACAGCAGGTTGGTCCATTTTCTAAGTTTAA740.10060225403428634No Hit
GTCTGGGGGCCGTGATTGCAGGAGCCCTGGCCGTGGAATTGATGGTATCTGT740.10060225403428634No Hit
TACAGGGACATAAAATATATTTATAGTACTCTAATTTATGTTGTAAAGTATG740.10060225403428634No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)