FastQCFastQC Report
Sat 31 Dec 2022
SRR5849668.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849668.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65341
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC5110.7820510858419675No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT5030.7698076246154788No Hit
TCAGCAGGGGACCCCCTAGCGCCGCTGGGCCTGCAGGTCTCTGTCGAGCAGC1200.18365191839733092No Hit
CTCGATGCGGGGAGTATCTGCTTGTTAAAATGGAAAAATGTGTCTTTAGAGC1130.17293888982415329No Hit
GACAATGGGTCATTTGTAGAGCTTCGAATTCCTTGCCCCTGAAAATAAGCTC1070.16375629390428675No Hit
TAGCTTGGGGACCTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTG1070.16375629390428675No Hit
GCATCTGGGGTGGGGTTTTTTCCTGTATGGGAAACCATTTATGCCAAGCTTT1040.15916499594435346No Hit
AGCACTGGGAGGTTCCAACATAGGAATTTTGGGGGGACACCAACATTTGGAT1000.1530432653311091No Hit
CTTATAGGGGATAACACATAATCTCCAACAGAAGTTACTGAATACATTCATA1000.1530432653311091No Hit
TCTCAGGGGCCTGTGAACACTGAAAGAGAAAGCCTAATGTAAAGTAGTGATG990.151512832677798No Hit
TCACAAGGGATACATGGATGATGATGTAATTGTGCAAGGTGATATTCTTGCC990.151512832677798No Hit
ATAAGCGGGATGTGGTTTTCAAAAAATTGTTGAAGACGTGACTTCATAGCAA980.14998240002448693No Hit
TCACCCGGGAAAATGACCTCACTTTTTTGAGGTGAGTGTGAAGAAAAGGACA980.14998240002448693No Hit
GGCTTAGGGGAAGCATTATTTTCCCAACACAAAAGAGCTTTTTTCTCGGTAT960.14692153471786473No Hit
CTTGCTGGGAATTCTTGCATGTTTGTATCTAATTTCTGAACGAATGAGCACA960.14692153471786473No Hit
GGTTTAGGGAGACAAGTAAATAAATATAAGACAAAAATCATTCATAATCTTA940.14386066941124256No Hit
TTCATAGGGAAGTAGATCAAACTAGCTTTTAAATGGATTAGCTTGTTCTCAA930.14233023675793147No Hit
AAGAGTGGGGATGCTTGACTTAAAGCTCTACAGCTAGCAGTAATAATCATAT920.14079980410462037No Hit
TTCGCAGGGAGTCGCGAGCGAACGACCAAGAGGGTGTTCGACTGCTAGAGCC920.14079980410462037No Hit
TGGCCAGGGACTATAATTTACTTTTGACTTAATGGGGCATAAATAAAACTTT920.14079980410462037No Hit
CTAACAGGATTGAATAAAATTTTATATAGGCAGTCCCCAGCCCTTTCCTCCT910.13926937145130927No Hit
TCCTAAGGGATAGCCACATACATCTAGATTAGACAAGAAGAAATATCTCAAG900.1377389387979982No Hit
CTGCTAGGGATCAACAAAGAGATAATATATTTTAAAAAGAAGTAAACAAATT890.1362085061446871No Hit
ACAGCCGGGATCTTTTTTGTTTTTGAGACAGAGTTTCGCTCTTGTTGCCTAA890.1362085061446871No Hit
TTCAGTGGGTGTCCTTGTCAATTTTTTTTACTGATAAAGCAGGATAAGTCTG880.134678073491376No Hit
AATGCAGGGTGAGGAATTCTCCTGGTGAAAAGATCAACTTACTGAAAAGAAG870.13314764083806493No Hit
TAACGAGGGAACAGTTAAGAAGCAAAGGCAATTATTAAAGATTTGGTGACTC860.13161720818475384No Hit
GCTGCTGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCC850.13008677553144274No Hit
CGTGACGGGCATCCTTTGAATTCACTCGGAGGCAAGGTGGTCATTTCTTATC850.13008677553144274No Hit
AACGGTCGGGGAGAAAAACTGACCAGCTACTATGGGAAATGTAAGACATGCT850.13008677553144274No Hit
GGTCTAGGGATTCAGGTTTATGAAGGCGAGCGTGCCATGACAAAGGATAACA850.13008677553144274No Hit
CGCTGTGGGCGATCTGATATAGGGACTTGTTTCTGAAATAAAATTCTGAAGG840.12855634287813164No Hit
AAAACAGGGATATGTGTTCTTGTTCTGATGTTGTTTCCTGAAATGATTTTTC840.12855634287813164No Hit
GCTTAGGGGTCGCCAGAGGTGCTTCACCCTGAGTTTTGTTTTGTATTGTTTT840.12855634287813164No Hit
TGCAAATGGGATCTAGTTAACATTTTAATTTTAAAATTGCCAACTTTTGGGA830.12702591022482054No Hit
TTCTTCGGGTACACAATTCTGACTCTGCTCAGTGGGTGCCAAAACTGTTGCT830.12702591022482054No Hit
GAACACGGGATTTGAAGAATGTCAGCAGAATTTAATGAAACTCCAGAAAGGA830.12702591022482054No Hit
TATAGAGGGAAGATCACCTTTGTGGATTTCCTTGCCTATGATGTCCTTGACA820.12549547757150945No Hit
TGCCTGGGGAAATCAACATTTCAAATTGGTAATAGAATGACCTTATTTATTA820.12549547757150945No Hit
TCGTACGGGGAAACATTTTCTAGGAAAAATAAAGGCAAACATACAATGTCAA810.12396504491819839No Hit
GTTTCAGGGAACATGGTGTGAGGACGGACTGGGAGCCGGTACAGACTCCAGT810.12396504491819839No Hit
TAGAGAGGGAGTACATAAATGCCTTTTTACAGCTTAATCATTTTGGTCTTCT800.12243461226488729No Hit
TCCGTGGGGAGTGCAGAGAATGTGGAAAATCCTTTATGTACAACTACCGACT790.12090417961157619No Hit
CTACGCGGGATTGACTTCTAATTGTGAATTAGTTTCCCTTTTCTACTTGTTT790.12090417961157619No Hit
GAGTCCGGGGTGGTCCTTTTACTCAAAATCGTTCTATATCTTGCCTGTCTGC790.12090417961157619No Hit
CTTCTTGGGGAGGAAGTAATGTATGGCCTTCCACGTGGATTAATTTCACTCT780.11937374695826511No Hit
ACACGTGGGACTCATAGTTTATAAAATGGCAGAAAATGTATACAAACTAATT780.11937374695826511No Hit
TATGTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA780.11937374695826511No Hit
ACGTGAGGGATGTGAACACATTGACTATTAAGCAAACTTAAGAGTGGTTGGA780.11937374695826511No Hit
AGGCAAGGGGTAGATGGAATTGCTTTTTTAATATGGGAAGATACATTTATTT770.11784331430495401No Hit
ACAGCAGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT770.11784331430495401No Hit
CACATGGGGAATGTTGATGACTGGATTCCTATTTATATAGTATTTGGGGGCT770.11784331430495401No Hit
GAATTAGGGGACAAAGGAATCTTTATTAGTCAGGATTAAAGTAGTCCATGAT770.11784331430495401No Hit
TAACCAGGGGTGTTAGAGCTTTCTTAATATTAAAAAAATTAATTGAAAAAGA770.11784331430495401No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCCTGTCTCTTATAC760.11631288165164291No Hit
CCATGGGTCTGAAGATGGGAAAGTGATACTCAGAACAAAAGAAAGTCATAAA760.11631288165164291No Hit
GAATGATGGGAGTTCAGTGCTGACCATTAAAAACTATCATGCTTGATACGGT760.11631288165164291No Hit
CGTTATGGGAGTGGGAGAGGGAAAATGACTCGGGACGCCATTGTAACGGTTC760.11631288165164291No Hit
CACCTGGGGGACACATCTGCTTTGTTATTTATAATAGAAAGCTAAATTTTAA750.11478244899833182No Hit
TCATGCGGGATTCGATATCTCAGCTCTAGAATAGCCCTGTGCTAAGAATGAC750.11478244899833182No Hit
GTAACAGGGATCCAGTTATTTAGTTTCAAGGTAGATTTCTTTGTTTTTATAT750.11478244899833182No Hit
GTCACAGGGCTATCGGGGGGGAAAATGAAATGGAAATGATGAGATGAGAAGA750.11478244899833182No Hit
AAAGTAGGGAATTGAGGTTAATATGCTTTCCTCAAAATCCATTGATTTAAAT740.11325201634502073No Hit
ACCAAAGGGATTAAACTATTGGATAGAGGAAAATGGTCCAAGATAAGAACAT740.11325201634502073No Hit
GAATCAGGGTATTTATAAGAAAAATGTGAATTTGGTGGTTTTATTCTATCGG730.11172158369170966No Hit
ATCTGTGGGGAGAGTCCTTGCCAGTTTTGTAACAAAGAGACCAACAATCATT730.11172158369170966No Hit
TAAAGCAGGGTCTTTTAGTTTAAGAAATATATTTATCTGAACTTTTGCCAAT730.11172158369170966No Hit
ACTAAAGGGGAGGTTCAGTGGATGATTTTTGTTCTGTCACTGATTTTTTCAC730.11172158369170966No Hit
TGATGCGGGAGTTCAAGATTTTCGGTATATAAAATCTGTCCTTTCCTACCTG730.11172158369170966No Hit
AGGTAAGGGATCCTTGATAATTCAAGGAAATATCCCTCCTGCCTAAGTTCCA730.11172158369170966No Hit
GAATCAGGGAGTTTATTATCAGCAAAATTATTTCTAATATTATTGGCTTACA730.11172158369170966No Hit
CGTTATGGGCTGGTGAACATTTTTCCAATTACATGGATATGAAACATTTTGT720.11019115103839856No Hit
TATCAAGGGGATCTGAATTGGGGATCATCCACACGGGGCAGTCGGCCCTCGG720.11019115103839856No Hit
ACTTATGGGTGCTATTTCTTTCTAGAATTTATTTGCCATTTTACCTGTTTGG720.11019115103839856No Hit
CTATCAGGGATATCCAGAGTTCATGCTTCTGGATGAAGACCGTGGCTTTTGG720.11019115103839856No Hit
CTGCATGGGTAGTGAAGCTACACTGTTTTATTTATGTGAACTTGCTCCTGCA710.10866071838508747No Hit
TTTAGGGGAATAGAGAATATCAATAAAGGGGGAAATTGTTAAAAAAGGAACA710.10866071838508747No Hit
AATGCAGGGGCAGGGCCTAACCTAAAAGGTTATTTATAAAATCAATAGCACT710.10866071838508747No Hit
CGGCCAGGGAGAATAACAAGAAAACTTCAGAATGTATCACTCTCCTGAAAAG700.10713028573177638No Hit
CACGCAGGGGTCTTCATTTAATTTGGTGGTGGTGAACTTTACTTGCTGATTT700.10713028573177638No Hit
TTATTTGGGACAGTAGGAAACGTCCCAATAAGGTAACTTTTTCAGAATTGAA700.10713028573177638No Hit
TACCATGGGGAAGAAAAAAAACCTCATATAATTTCAGCTTTTTGGAAGAAAG700.10713028573177638No Hit
TTTAGAGGGTGTCTTACAATTGAATTCATTTTGCTGTTGAATGGGAAAGCAG700.10713028573177638No Hit
GGATATGGGGAATCCCCAGGGACATTCCATCATTGCAATAGCTCAGATTTTT700.10713028573177638No Hit
CCACTGGGGATTGTAGCTTATATAACATTTTCTAAATTTAGATATCCCATCA700.10713028573177638No Hit
TTCAGAGGGGTTCCTACAGTTTTCCTTTAAAGTGTTTTGAGATTAGAAACAA700.10713028573177638No Hit
TTTTTTGGGACAGCCTCAGCTCCAGGCTATCTGGCAATAACAAAGAAAGTGG700.10713028573177638No Hit
AGACGGGAAGGAAAAAGGCATGCTTTCTGACTGACATGATCTTTTGCAATAC700.10713028573177638No Hit
AATCTGGGACCTGACAAGTCCTAACCATGATTATTAAATCTAAGAGAGACTG700.10713028573177638No Hit
TGCTTGGGGAGACTGAAAGTGAAGGAATGGACAAAGATATCCCATGCAAATT690.10559985307846528No Hit
ATAACAGGGAGCCTTATAAAAGGGAAGGTAAATGGCATAAATATGGTCGCAC690.10559985307846528No Hit
CCCCCGGGGGGGCAGCCAGCAGTACCGGGCGCTCACGGTGCCCGAGCTCACC690.10559985307846528No Hit
TGTGGCGGGTATTGCTACGGAAACTGAGAACCAAGGGGCAGAAAGATAACCT690.10559985307846528No Hit
AACGATGGGACCGAGCACATGTTGTTGTGTTAGTTTAAAACGATAATAAAAA690.10559985307846528No Hit
GTTAAAGGGATTAAGACCACGGAGCAGCTCATCGAGGCCGTCAACAACGGTG690.10559985307846528No Hit
TGAGTGGGGAGACGTTCAAATGTTTAATGGTATAGTCTGACCTAGTGAAACA680.10406942042515419No Hit
AACAAAGGGATAGCACTCAATACGAAATATTCTAGGGATATTTTACTGTAGT680.10406942042515419No Hit
TTCATGGGGAAAGAGACTGAGAGAAATTAATAGTATGATACGCACAGGAGAA680.10406942042515419No Hit
GCCCCGCCTCAAGCGGCGCGACTTCACCCCCGCCGAGCTGCGGCGCTTCGAC680.10406942042515419No Hit
TCATTTGGGGATTGTATTGAGGATTAGTCTCAGTTGGAAAGCCAACTACAGA680.10406942042515419No Hit
TCAGCTGGGAAGAATACAAGGTGGCTTACTATTTTTTCTTTCTTTGAGAAGT680.10406942042515419No Hit
TATGGTGGGGGAATTACGCTCGCAGAGCTGCGGGCTCTCATGGAGCTCAGGT680.10406942042515419No Hit
TTTGGCGGGGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTAT670.1025389877718431No Hit
TACAGAGGATTCTTCACATTTAATGTTTGATAGTTCAATTTCTTGAGCTGAA670.1025389877718431No Hit
TACCATGGGGTACACTTGGTGTAACTGTATGGAAATATAGTTTCTAGCTTTT670.1025389877718431No Hit
AGGAATGGGATAGCACATTTCTTGTGTTAACCATCCCTTTTTGAGCGTGACT660.101008555118532No Hit
CTACTAGGGGGGCCAACCTTGACTACCCCGGCCCAGGGCTCACCTACAAGAC660.101008555118532No Hit
TCTGTGGGGCTTCAAAACTAAGGAGTTGCAGAAAAAAATGGATTTCACAGAG660.101008555118532No Hit
CATCACGGGACCCAAGTTTCATCCATGTTGAATGGTACAAAATATTTCTGTG660.101008555118532No Hit
GCGCGGGGGCAGTTCTTTTAGGATTATAGGTTGCAAATTATCCAAATATATA660.101008555118532No Hit
GTTTGTGGGTTGGCAGAAGGACCGTGCCCGGCGGCCTCATTTTGACCAGCTG660.101008555118532No Hit
GCTTCTGGGATAGTGTCTCACTACATATTAATATTTATTGTTCAATAAATGA660.101008555118532No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)