FastQCFastQC Report
Sat 31 Dec 2022
SRR5849667.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849667.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64450
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC4940.7664856477889838No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT4420.6858029480217223No Hit
TCACAAGGGATACATGGATGATGATGTAATTGTGCAAGGTGATATTCTTGCC1140.1768813033359193No Hit
GACAATGGGTCATTTGTAGAGCTTCGAATTCCTTGCCCCTGAAAATAAGCTC1080.16757176105508145No Hit
CGTGACGGGCATCCTTTGAATTCACTCGGAGGCAAGGTGGTCATTTCTTATC1080.16757176105508145No Hit
GCTGCTGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCC1070.16602017067494182No Hit
TCATTTGGGGATTGTATTGAGGATTAGTCTCAGTTGGAAAGCCAACTACAGA1020.1582622187742436No Hit
AGCACTGGGAGGTTCCAACATAGGAATTTTGGGGGGACACCAACATTTGGAT1020.1582622187742436No Hit
TAGAGAGGGAGTACATAAATGCCTTTTTACAGCTTAATCATTTTGGTCTTCT990.15360744763382467No Hit
TCCTAAGGGATAGCCACATACATCTAGATTAGACAAGAAGAAATATCTCAAG970.15050426687354537No Hit
GCATCTGGGGTGGGGTTTTTTCCTGTATGGGAAACCATTTATGCCAAGCTTT970.15050426687354537No Hit
TCAGCAGGGGACCCCCTAGCGCCGCTGGGCCTGCAGGTCTCTGTCGAGCAGC960.14895267649340574No Hit
GCCCCGCCTCAAGCGGCGCGACTTCACCCCCGCCGAGCTGCGGCGCTTCGAC930.1442979053529868No Hit
GAACACGGGATTTGAAGAATGTCAGCAGAATTTAATGAAACTCCAGAAAGGA930.1442979053529868No Hit
CGTTATGGGCTGGTGAACATTTTTCCAATTACATGGATATGAAACATTTTGT910.14119472459270754No Hit
ACAGCAGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT910.14119472459270754No Hit
CTTCTTGGGGAGGAAGTAATGTATGGCCTTCCACGTGGATTAATTTCACTCT900.13964313421256788No Hit
ACTAAAGGGGAGGTTCAGTGGATGATTTTTGTTCTGTCACTGATTTTTTCAC900.13964313421256788No Hit
GTAACAGGGATCCAGTTATTTAGTTTCAAGGTAGATTTCTTTGTTTTTATAT900.13964313421256788No Hit
CTAACAGGATTGAATAAAATTTTATATAGGCAGTCCCCAGCCCTTTCCTCCT890.13809154383242825No Hit
CTCGATGCGGGGAGTATCTGCTTGTTAAAATGGAAAAATGTGTCTTTAGAGC890.13809154383242825No Hit
GGTTTAGGGAGACAAGTAAATAAATATAAGACAAAAATCATTCATAATCTTA890.13809154383242825No Hit
TCGTACGGGGAAACATTTTCTAGGAAAAATAAAGGCAAACATACAATGTCAA880.13653995345228861No Hit
TCCGTGGGGAGTGCAGAGAATGTGGAAAATCCTTTATGTACAACTACCGACT880.13653995345228861No Hit
TATCAAGGGGATCTGAATTGGGGATCATCCACACGGGGCAGTCGGCCCTCGG880.13653995345228861No Hit
ATAAGCGGGATGTGGTTTTCAAAAAATTGTTGAAGACGTGACTTCATAGCAA860.13343677269200932No Hit
CTTGCTGGGAATTCTTGCATGTTTGTATCTAATTTCTGAACGAATGAGCACA860.13343677269200932No Hit
TATGGTGGGGGAATTACGCTCGCAGAGCTGCGGGCTCTCATGGAGCTCAGGT850.13188518231186966No Hit
GTTATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC840.13033359193173003No Hit
TTCAGTGGGTGTCCTTGTCAATTTTTTTTACTGATAAAGCAGGATAAGTCTG830.12878200155159036No Hit
TGCAAATGGGATCTAGTTAACATTTTAATTTTAAAATTGCCAACTTTTGGGA820.12723041117145073No Hit
TTCTTCGGGTACACAATTCTGACTCTGCTCAGTGGGTGCCAAAACTGTTGCT820.12723041117145073No Hit
TTCTAGGGGGAAGGTGTAGTTCAAGTAATTACTACAAATAATTAAAGCCCTA820.12723041117145073No Hit
AACGGTCGGGGAGAAAAACTGACCAGCTACTATGGGAAATGTAAGACATGCT810.1256788207913111No Hit
GGTCTAGGGATTCAGGTTTATGAAGGCGAGCGTGCCATGACAAAGGATAACA810.1256788207913111No Hit
GCTTAGGGGTCGCCAGAGGTGCTTCACCCTGAGTTTTGTTTTGTATTGTTTT810.1256788207913111No Hit
TTCATAGGGAAGTAGATCAAACTAGCTTTTAAATGGATTAGCTTGTTCTCAA810.1256788207913111No Hit
GGATCCGGGGGGATTGTGGTAAGATGTGTTTCCGTGCATGTCAGTGCTGCTA800.12412723041117144No Hit
CTACGCGGGATTGACTTCTAATTGTGAATTAGTTTCCCTTTTCTACTTGTTT800.12412723041117144No Hit
TTGGCTGGGGCAAATGGAACCAAAAAAAGAGCCCATATATGCCAGACAATCC790.1225756400310318No Hit
CACATGGGGAATGTTGATGACTGGATTCCTATTTATATAGTATTTGGGGGCT790.1225756400310318No Hit
TACAGAGGATTCTTCACATTTAATGTTTGATAGTTCAATTTCTTGAGCTGAA790.1225756400310318No Hit
GTTTCAGGGAACATGGTGTGAGGACGGACTGGGAGCCGGTACAGACTCCAGT790.1225756400310318No Hit
TGCTTGGGGTTCTTCCTTCTCGCCTAACGCTGCCAACATGGTGTTCAGGCGC780.12102404965089217No Hit
TTATTTGGGACAGTAGGAAACGTCCCAATAAGGTAACTTTTTCAGAATTGAA780.12102404965089217No Hit
CATTAAGGGCAGGGCATGCCGGTCATCCTGAGACCTTCCTTTAACCTCCTTT780.12102404965089217No Hit
GAGTCCGGGGTGGTCCTTTTACTCAAAATCGTTCTATATCTTGCCTGTCTGC780.12102404965089217No Hit
AGACGGGAAGGAAAAAGGCATGCTTTCTGACTGACATGATCTTTTGCAATAC780.12102404965089217No Hit
GTATGGGGGATACCTTCCTTAGAGAAATGTCAATTCATATAACTTGCACGTT780.12102404965089217No Hit
AGCAGGGGGATAGATCATCTTGAAGTATTATTTCACATTGAAAAGAAGAAAA770.11947245927075253No Hit
GGTCTAGGGGACATAGCAATTCTACTCCTGTATATGTGCCCAAGAAAGATGA770.11947245927075253No Hit
AAGAGTGGGGATGCTTGACTTAAAGCTCTACAGCTAGCAGTAATAATCATAT770.11947245927075253No Hit
CTCACGGGCTTATTTTACTTAATAATGACCCCAAAGTGCAAGAGTACTGATG770.11947245927075253No Hit
TACCTTGGGCTAGCATTGGGTACAACTACTTAAAAAATATAGTCAATAGGTT770.11947245927075253No Hit
CAGCAAGGGGCCTGTAATCCCTGCTGCTTGGGAGGCTGAAACAGGAGAATCG760.11792086889061287No Hit
CAAGTTGGGGTGCTAAGTTAAAATACAGTTTAGTTATTTGCTTTAAAATAAA760.11792086889061287No Hit
AGACCCGGGGACTCACAGTTCTGCAGAGCTGGGGAGGCCTCAGAAAACTTAC760.11792086889061287No Hit
GTCACAGGGCTATCGGGGGGGAAAATGAAATGGAAATGATGAGATGAGAAGA760.11792086889061287No Hit
GAATGATGGGAGTTCAGTGCTGACCATTAAAAACTATCATGCTTGATACGGT760.11792086889061287No Hit
GAATCAGGGAGTTTATTATCAGCAAAATTATTTCTAATATTATTGGCTTACA760.11792086889061287No Hit
ACGTGAGGGATGTGAACACATTGACTATTAAGCAAACTTAAGAGTGGTTGGA760.11792086889061287No Hit
TGATTTGGGAACGTCGAGATCTTCAACATCCTTTTCCATAAAAGGAAAGAAT750.11636927851047324No Hit
CTTATAGGGGATAACACATAATCTCCAACAGAAGTTACTGAATACATTCATA750.11636927851047324No Hit
TTCGCAGGGAGTCGCGAGCGAACGACCAAGAGGGTGTTCGACTGCTAGAGCC740.11481768813033359No Hit
TACCATGGGGAAGAAAAAAAACCTCATATAATTTCAGCTTTTTGGAAGAAAG740.11481768813033359No Hit
TAGCTTGGGGACCTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTG740.11481768813033359No Hit
TGTCGGGGAGCCGTTTTCCTTCAGGAACATAGATGCAAATTTCCTCAAAAAT740.11481768813033359No Hit
TTTGGCGGGGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTAT730.11326609775019396No Hit
AACAAAGGGATAGCACTCAATACGAAATATTCTAGGGATATTTTACTGTAGT730.11326609775019396No Hit
TATAGAGGGAAGATCACCTTTGTGGATTTCCTTGCCTATGATGTCCTTGACA730.11326609775019396No Hit
AGCAAAGGGATAGAAGATTTGGCCTTCTACTCTAGTACAGTAGTATCTCTAT730.11326609775019396No Hit
ACACGTGGGACTCATAGTTTATAAAATGGCAGAAAATGTATACAAACTAATT730.11326609775019396No Hit
TGAGTGGGGAGACGTTCAAATGTTTAATGGTATAGTCTGACCTAGTGAAACA720.1117145073700543No Hit
CGCTGTGGGCGATCTGATATAGGGACTTGTTTCTGAAATAAAATTCTGAAGG720.1117145073700543No Hit
CACGAGGGGAGGAGGAGAAGACAAGGAGGAAAGAGAAGGAGGAAAGAAGAAG720.1117145073700543No Hit
TGGCCAGGGACTATAATTTACTTTTGACTTAATGGGGCATAAATAAAACTTT720.1117145073700543No Hit
TTGCCAGGAAGTCCTTGTTCCTGGTGAACCACAGTTAGGGTGTTTTGTTTAT720.1117145073700543No Hit
ACAGCCGGGATCTTTTTTGTTTTTGAGACAGAGTTTCGCTCTTGTTGCCTAA720.1117145073700543No Hit
GCTTCTGGGATAGTGTCTCACTACATATTAATATTTATTGTTCAATAAATGA720.1117145073700543No Hit
TCATGCGGGATTCGATATCTCAGCTCTAGAATAGCCCTGTGCTAAGAATGAC710.11016291698991466No Hit
GGCTTAGGGGAAGCATTATTTTCCCAACACAAAAGAGCTTTTTTCTCGGTAT710.11016291698991466No Hit
AATACAGGGAAATTTTTAAGTCTGTAAATATATTTTAAAGTTATATAATAAT710.11016291698991466No Hit
TCACCCGGGAAAATGACCTCACTTTTTTGAGGTGAGTGTGAAGAAAAGGACA710.11016291698991466No Hit
GATCATGGGCAGCCAGAACGCCTGAACGCAGGCACATACTTCCTATTCTACA710.11016291698991466No Hit
TGCCTGGGGAAATCAACATTTCAAATTGGTAATAGAATGACCTTATTTATTA710.11016291698991466No Hit
GACTTTAGGGTCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAA710.11016291698991466No Hit
TTTAGAGGGTGTCTTACAATTGAATTCATTTTGCTGTTGAATGGGAAAGCAG700.10861132660977503No Hit
CCACTGGGGATTGTAGCTTATATAACATTTTCTAAATTTAGATATCCCATCA700.10861132660977503No Hit
GCTGAAGGGATCAACTCAGTAGCCCACACCCAGTTATCTGGGAGCTGATGGT700.10861132660977503No Hit
AATCTGGGACCTGACAAGTCCTAACCATGATTATTAAATCTAAGAGAGACTG700.10861132660977503No Hit
GCACAAGGGATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTC700.10861132660977503No Hit
AAAGTAGGGAATTGAGGTTAATATGCTTTCCTCAAAATCCATTGATTTAAAT690.10705973622963537No Hit
TAAGGAGGAGGTACCTGTTTGGCCCAAGAGTCCTAGCTTTTGGCCTATCTTG690.10705973622963537No Hit
ACCAAAGGGATTAAACTATTGGATAGAGGAAAATGGTCCAAGATAAGAACAT690.10705973622963537No Hit
TAAAGCAGGGTCTTTTAGTTTAAGAAATATATTTATCTGAACTTTTGCCAAT690.10705973622963537No Hit
ATAACAGGGAGCCTTATAAAAGGGAAGGTAAATGGCATAAATATGGTCGCAC690.10705973622963537No Hit
AGCGTGGGGCCCTCCAGCTTTTAGAGCCGAAGATCTTTTTTTCTGAGCCCCA690.10705973622963537No Hit
TCGACAGGGATATATATATTTGATAGTTTGTGGAATAATATCCCCCAGTATT690.10705973622963537No Hit
TGTAGCGGGCCGATCTGATAGAGGGCAGTAATCATACAGTTTTGCACTCAAT690.10705973622963537No Hit
AATGCAGGGGCAGGGCCTAACCTAAAAGGTTATTTATAAAATCAATAGCACT690.10705973622963537No Hit
TCTCAGGGGCCTGTGAACACTGAAAGAGAAAGCCTAATGTAAAGTAGTGATG680.10550814584949574No Hit
CACTTAGGGCCACTTCCAAAGATGCAAAAGGCCTAATTGAATCAAGTCTCCT680.10550814584949574No Hit
TTTTGTGGGGCCTCATGGAGAGCTGAGAAAGATAGGGAATCTCTCCGTCGTA680.10550814584949574No Hit
ACTCTCGGGGGTTGTAGCATACTCAGTACTTCATATCTGTTATGGCTGAATA680.10550814584949574No Hit
ATAGATGGGGGGAAACAAATCTGTCCAAAAAGAAAGTCTGTGAGAAGCTAAG680.10550814584949574No Hit
GGATCCGGGTATGGCGGGGATGTGTAATGGATGTTTTTAATTTTTTCTAGTT680.10550814584949574No Hit
TAACGAGGGAACAGTTAAGAAGCAAAGGCAATTATTAAAGATTTGGTGACTC670.10395655546935609No Hit
GCTGATGGGCATCCCAGATTTTCCTCTACATGCATGTGCCCTCTTTTAAGAA670.10395655546935609No Hit
GCTAGGGGGTTCTGCATCCACCGATTCAAACAACTGTGGAACAAAATTGTCA670.10395655546935609No Hit
CGTTATGGGAGTGGGAGAGGGAAAATGACTCGGGACGCCATTGTAACGGTTC670.10395655546935609No Hit
AGAGTTGGGATGAATCATCAATAAAAACAGTTGCTTCTGTTTGCTGTTGACA670.10395655546935609No Hit
TCGGTAAGGGACTCATTAGACTGCTGCCTTTAGTGTTATTGCTTCAAGTTTC670.10395655546935609No Hit
CGTCTCGGGCCGATCTGTCACTGGGACAGGAGTTACTAGAGTCACTATCCGG660.10240496508921645No Hit
TTAAGTGGGGCTGCACTGCATCAGTATCTTACTAAAAATGTGAAATGAAAGG660.10240496508921645No Hit
TGAACTGGGGGCACACAGCGAATAGGCTGGTTCTCCATGAGGATTGTATTTT660.10240496508921645No Hit
CATCACGGGACCCAAGTTTCATCCATGTTGAATGGTACAAAATATTTCTGTG650.10085337470907679No Hit
GTTAGGGGGCAATAGTACTTGCCGCAGTACTCTTAAAACTAGGCGGCTATGG650.10085337470907679No Hit
TGTGGCGGGTATTGCTACGGAAACTGAGAACCAAGGGGCAGAAAGATAACCT650.10085337470907679No Hit
CTTTAGGGGGAAGTCAATCATTTTTCCTATAGGGCTCTGTTTCCTTTTCTTT650.10085337470907679No Hit
GCTATAGGGCCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATG650.10085337470907679No Hit
ATTGTTGGGCAATTAATGCTGAACATGAAGAGTAAAGTATTTATCTGAAAGA650.10085337470907679No Hit
TTCGTGGGGCACGCCATTGCACTCCCACCCGGGCAACAGTGCGAGACCCCAT650.10085337470907679No Hit
AGACCAGGGACGGGTGTTTTCTAAACATTTGAAGTTTTACGATTAGCTTTAA650.10085337470907679No Hit
TACCATGGGGTACACTTGGTGTAACTGTATGGAAATATAGTTTCTAGCTTTT650.10085337470907679No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)